A novel 2D Co-MOF@MnO2 composite material with enhanced oxidase-like activity for rapid uric acid detection and portable sensor device development

A novel two-dimensional metal-organic framework (2D MOF), 2D Co-MOF@MnO 2 , with oxidase-like activity was successfully prepared via a solvothermal method, and its mimetic oxidase-like activity was investigated in detail. Its kinetic parameters K m and V max were determined to be 0.048 mM and 5.897 × 10 − 7 Ms − 1 , respectively. Taking advantage of the material’s ability to catalyze the oxidation of 3,3’,5,5’-tetramethylbenzidine (TMB) and the inhibitory effect of uric acid (UA) on this oxidase-like activity, we designed and constructed a portable colorimetric sensing device for the rapid detection of UA. This device enabled UA detection within 3 min at room temperature. The proposed system showed a linear response in the concentration range 5-200 µM with a detection limit of 4.98 µM, which was comparable to or even better than several previously reported colorimetric methods. This proof-of-concept study presented a novel and efficient approach that had potential for future point-of-care (POCT) applications for uric acid detection, and may offer new insights into early disease diagnosis, health monitoring, and biomedical research. Graphical abstract The alternative text for this image may have been generated using AI.