摘要
An underutilized Pyracantha fortuneana resource was valorized into a bioactive pectin. • Pectin polysaccharide from P. fortuneana (PFP) is a highly methylated, galacturonic acid–rich pectin. • PFP demonstrated protective effects against DSS-induced colitis in vivo. • PFP improved epithelial barrier integrity and regulated inflammatory responses. Valorization of underutilized forestry resources into bioactive polymers is a sustainable strategy. In this study, a novel pectin polysaccharide was isolated from Pyracantha fortuneana (PFP) using optimized acid extraction combined with ultrafiltration, and its structural characteristics and intestinal protective effects were systematically investigated. Structural analysis revealed PFP to be an acidic, highly methylated polysaccharide predominantly composed of galacturonic acid, with a molar ratio of rhamnose, galactose, arabinose and galacturonic acid of 2.79:3.95:4.31:88.09. FT-IR, methylation and NMR data indicated that the PFP backbone consists of a repeating unit of →2)-α- L -Rha p -(1→4)-α- D -Gal p A-(1→4)-α-Gal p A-OMe-(1→4)-α-Gal p A-(1→ forming a HG-type structure, while its side chains are mainly constituted of →5)-α- L -Ara f -(1→, →4)-β-Gal p -(1→4)-β-Glc p -(1→, and →6)-β-Gal p -(1→4)-β-Gal p -(1→ branched linkages. In vivo studies showed that PFP significantly ameliorated DSS-induced colitis in mice, as evidenced by improved intestinal barrier function, reduced weight loss and disease activity index, and preserved colonic architecture. Furthermore, PFP modulated gut microbiota by enriching the phylum Bacteroidetes and Bacteroides genus, reducing Proteobacteria phylum, suppressing oxidative stress and inflammatory cytokine production. These findings highlight the potential of PFP as a natural functional ingredient for intestinal health applications in food and nutraceutical formulations. Download: Download high-res image (179KB) Download: Download full-size image
危险品化学品经营许可证（带存储）