A multifunctional hybrid delivery system of sodium alginate-based nanoparticles-hydrogel beads “double fence” effect for pea peptide

FA-SA conjugate with excellent antioxidant activity was prepared. • FA-SA-PP NPs exhibited good antioxidant and DPP-IV inhibit activity. • SA-based HBs achieved the intestinal-targeted release of FA-SA-PP NPs. • NPs provide I-level defense system and HBs provide II-level defense system for PP. Pea peptides (PP) with hypoglycemic activity were prone to decrease activity due to their sensitivities to pH, temperature and enzymes. Herein, ferulic acid modified sodium alginate conjugate was prepared and employed as the carrier for fabricating FA-SA-PP NPs with the PP encapsulation efficiency (EE) of 99.69%, which formed the I-level fence for protecting PP. Further, SA-based hydrogel beads loaded with FA-SA-PP NPs (EE: 99.87%) were prepared for forming the II-level fence to protect PP. Results showed that FA-SA-PP NPs@SA HBs had good stability in SGF, and gradually swelled and released FA-SA-PP NPs in SIF. Besides, the digested products of FA-SA-PP NPs@SA HBs still showed good antioxidant and DPP-IV inhibitory activities. Overall, the “double fence” effect protection mechanism can achieve the combination of antioxidant and hypoglycemic functions, and realize the intestinal-targeted release of PP, which shows great application potential in reducing oxidative stress damage and nutrition intervention to diabetes. Download: Download high-res image (162KB) Download: Download full-size image