A Superparamagnetic Platform Enhanced with Metal-Modified Carbon Dots for Rapid Dual-Modal Detection of Staphylococcus aureus

Staphylococcus aureus(S. aureus) is a significant pathogen that causes foodborne diseases. Meat, with its abundant nutrients and high water activity, constitutes an ideal niche forS. aureuscolonization. Numerous studies have shown that human digestive tract diseases caused by consuming meat products contaminated withS. aureusoccur frequently. It is of great significance to strictly monitorS. aureusin meat matrices. A novel biosensor employing dual-signal output was developed through the combination of efficient magnetic separation and dual-modal precise detection. Designed for the rapid enrichment ofS. aureus, it significantly boosts detection sensitivity and accuracy, thereby enabling the earlier identification of potential contamination sources. This method uses vancomycin-modified magnetic beads as the capture element, and aptamer-modified nanozymes as the signal element. After magnetic separation, the 3,3′,5,5′-tetramethylbenzidine color reaction can be used to quickly and sensitively detectS. aureus. It achieved a detection limit as low as 10 cfu/mL. Moreover, this dual-signal sensor based on efficient magnetic separation can sensitively detectS. aureusin meat products, thus showing good application prospects in food matrices and further improving the reliability and specificity of detection.