An interface-confined ultrabright AIE nanoparticle-enhanced lateral flow immunoassay platform for full-range and accurate CRP detection

C-reactive protein (CRP) is a key biomarker for both infectious diseases and cardiovascular and cerebrovascular disorders. Thus, accurate full-range quantification is critical for early screening and timely intervention. However, limited sensitivity in conventional lateral flow immunoassays (LFIAs) and aggregation-induced quenching in traditional fluorescent probes collectively restrict analytical performance. In this work, a fluorescent LFIA platform based on P(St-AA)@AIENPs is established via an interfacial confinement–assisted loading strategy, enabling effective incorporation of aggregation-induced emission (AIE) luminogens into a poly(styrene-acrylic acid) matrix and delivering a detection limit of 0.66 ng/mL, a broad working range of 0–2000 ng/mL, high specificity, and strong correlation with ELISA (R 2  = 0.995) within a 15 min assay. Overall, this platform offers a rapid, accurate, and portable solution for full-range CRP detection. It is particularly suitable for community-level screening and home-based testing, supporting improved disease management and a reduced public healthcare burden.