Metal-Polyphenol Network-Stabilized Selenium/Prussian Blue Nanoplatform for Multienzyme Mimicking and Photothermal Wound Healing

Bacterial infection and oxidative stress represent significant clinical challenges in wound management. Upon tissue damage, bacterial colonization triggers excessive reactive oxygen species (ROS) production and persistent inflammation, forming a vicious cycle that impairs wound healing. The development of nanomedicines integrating antibacterial and antioxidant functionalities is a promising therapeutic strategy to concurrently combat pathogen invasion and oxidative damage. Herein, we fabricated a core–shell nanoplatform (PB@Se@TA-Fe) through a green and mild strategy, where tannic acid (TA) plays a dual role: reducing Na2SeO3 to generate an intermediate selenium layer on Prussian blue (PB) cores and coordinating with Fe2+ to form a stabilizing polyphenolic metal network as the outer shell. This TA-Fe shell firmly stabilizes the Se interlayer, yielding structurally robust nanoparticles with a sandwich-like architecture. The core-interlayer-shell configuration enables synergistic integration of all components, conferring superoxide dismutase (SOD)-like, catalase (CAT)-like, glutathione peroxidase (GPx)-like, and peroxidase (POD)-like enzymatic activities for ROS elimination. Under near-infrared (NIR) irradiation, the nanoplatform exhibits photothermal conversion ability and broad-spectrum antibacterial efficacy against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). In vitro, PB@Se@TA-Fe alleviates H2O2-induced oxidative damage. In a bacterial infection wound model, the nanoplatform significantly accelerates wound healing by eliminating pathogens and reducing oxidative stress, indicating its potential for oxidative stress-related disorders and infectious wounds.