A cell membrane inspired multifunctional hydrogel loaded with ginsenoside Rh1 for S. aureus infected wound healing

Graphene oxide enhanced hydrogel enables tunable mechanics. • Sustained Rh1 release promotes angiogenesis via VEGF/CD31 upregulation. • Hydrogel boosts anti-inflammatory cytokines (IL-4, IL-10) for faster healing. • The water gel dressing has an antibacterial rate of up to 98.69% against S. aureus . During the healing process of skin wounds, there are challenges such as slow tissue regeneration and bacterial infections. This paper has developed a drug-loaded composite hydrogel dressing that can promote angiogenesis, inhibit bacteria, and reduce inflammation. The hydrogel is composed of gelatin (Gel) and acrylamide (AM), and is formed into a biomimetic cell membrane structure through cross-linking. By adding different concentrations of graphene oxide (GO), the tensile strength of the hydrogel dressing can be significantly increased (from 57.54 KPa to 95 KPa), and the adhesion strength (shear strength) can be effectively enhanced (16.44 KPa). Additionally, Panax ginsenoside Rh1 has been successfully encapsulated in the hydrogel, achieving local controlled release. In vivo evaluation shows that the enhanced matrix by graphene oxide and the sustained release of Panax ginsenoside Rh1 have an antibacterial rate of up to 98.69% against S. aureus . It also stimulates the proliferation, migration, and tube formation of endothelial cells, thereby achieving strong vascularization at the wound site. Moreover, it can enhance the secretion capacity of anti-inflammatory factors (IL-4 from 19.54 to 105.61 pg/mL, IL-10 from 151.94 to 339.98 pg/mL), thereby accelerating the wound healing process. This hydrogel dressing provides a promising strategy for achieving high-quality wound repair. Download: Download high-res image (223KB) Download: Download full-size image