摘要
Surfactant-free tocotrienols (T3) self-assemble into nanoparticles (179–236 nm). • T3 nanoparticles with BLG or CMS exhibit high encapsulation efficiency (82–99%). • BTNPs and CTNPs showed distinct interfacial assembly mechanisms. • BTNPs demonstrate superior colloidal stability and bioaccessibility. • BTNPs and CTNPs enhance antioxidant activity of T3 via improved dispersibility. Tocotrienols (T3) are bioactive compounds with diverse biological activities, but their lipophilicity and poor bioavailability limit their application in the food field. In this study, surfactant-free self-assembled T3 nanoparticles (STNPs), β-lactoglobulin (BLG)-T3 co-assembled nanoparticles (BTNPs) and carboxymethyl chitosan (CMS)-T3 co-assembled nanoparticles (CTNPs) were comparatively evaluated. Both BTNPs and CTNPs could transform oily T3 into a solid-like lyophilized powder with excellent reconstitution properties. BTNPs exhibited higher encapsulation efficiency (EE, 82%–99%) and loading capacity (LC, 9–25 g/100 g), along with smaller particle size (143–198 nm) than STNPs (179–236 nm). Isothermal titration calorimetry revealed that BLG exhibited significantly higher apparent affinity toward T3 compared with CMS. Spectroscopic investigations suggested intermolecular interactions between T3 and carriers. Importantly, BTNPs exhibited enhanced colloidal stability under varying pH, ionic strength, and storage conditions compared with STNPs and CTNPs. The bioaccessibility of T3 was largely enhanced in all nanoparticles systems. Furthermore, compared with STNPs, BTNPs and CTNPs exhibited stronger hydrophilic-radical scavenging capacities and more effectively alleviated H 2 O 2 -induced ROS accumulation in HepG2 cells. These findings highlight the role of carrier-mediated colloidal properties in modulating the stability and antioxidant efficacy of encapsulated T3. Download: Download high-res image (163KB) Download: Download full-size image
危险品化学品经营许可证（带存储）