Amino-Functionalized Mesoporous SiO2 Hollow Nano-Doughnuts for Enhanced Gas Adsorption and Specific Foodborne Pathogen Recognition in Point-of-Care Detection

en This link goes to a English section zh This link goes to a Chinese section Developing rapid, precise gas sensors for Listeria monocytogenes ( LM ) biomarker detection faces challenges due to suboptimal gas-solid interactions. Herein, we report the rational synthesis of amino-functionalized mesoporous SiO 2 hollow nano-doughnuts (MS-2-NH 2 -2) via modification with (3-aminopropyl) trimethoxysilane (AAPTMS), which enables exceptional detection performance for the LM biomarker 3-hydroxy-2-butanone (3H2B). The MS-2-NH 2 -2-based quartz crystal microbalance (QCM) sensors exhibited high sensitivity (1980 Hz@50 ppm 3H2B), effective selectivity, low detection limit (100 ppb), rapid response/recovery kinetics (9/10 s), good repeatability, and outstanding humidity resistance. Furthermore, to enhance the selectivity performance of MS-2-NH 2 -2 in practical applications, the K-nearest neighbor algorithm is implemented to identify 3H2B, single interfering gases, and mixed gases. These performances stem from AAPTMS-derived amino groups, mesoporous structure, and doughnut-like hollow morphology, which enhance gas-material interactions. Temperature-programmed experiments revealed a −45.18 kJ mol −1 enthalpy change, confirming reversible chemisorption between MS-2-NH 2 -2 and 3H2B. In situ DRIFTS confirmed selective detection driven by –NH 2 –carbonyl interactions. Additionally, practical validation in complex food matrices demonstrates the sensor's capability for real-time 3H2B monitoring with high accuracy and reliability, suggesting its potential as a rapid, field-deployable screening tool for LM prioritization in food safety applications. 摘要 en This link goes to a English section zh This link goes to a Chinese section 在开发针对单增李斯特菌( Listeria monocytogenes , LM )生物标志物检测的快速响应与精准识别气体传感器过程中,因气体分子与固体传感器界面间的相互作用效率低下,导致检测灵敏度与特异性提升面临显著技术挑战。本研究通过(3-氨丙基)三甲氧基硅烷(AAPTMS)改性,成功合成了氨基功能化空心介孔SiO 2 纳米甜甜圈(MS-2-NH 2 -2),实现了对 LM 生物标志物3-羟基-2-丁酮(3H2B)的高效检测。基于MS-2-NH 2 -2的石英晶体微天平(QCM)传感器表现出高灵敏度(1980 Hz@50 ppm 3H2B)、高选择性、低检测限(100 ppb)、快速响应/恢复动力学(9/10秒)、良好重复性和优异抗湿性。为提升MS-2-NH 2 -2在实际应用中的选择性表现,引入K近邻算法实现了对3H2B、单一干扰气体及混合气体的精准识别。这些优异性能源于AAPTMS衍生的氨基基团、介孔结构及甜甜圈状空心形貌的协同作用,增强了气体-材料相互作用。程序升温实验计算得出的焓变值(−45.18 kJ mol −1 ),证实了MS-2-NH 2 -2与3H2B之间存在可逆化学吸附。原位漫反射红外傅里叶变换光谱(DRIFTS)验证了氨基-羰基相互作用驱动的特异性检测机制。在复杂食品基质中的实际应用验证表明,该传感器可实现3H2B的即时检测,展现出高精度与可靠性,彰显了其作为快速、精准且可现场部署的 LM 检测工具在食品安全领域的巨大应用潜力。