摘要
SP0–1, a neutral polysaccharide fraction isolated from sea buckthorn berries ( Hippophae rhamnoides L.), was purified and structurally characterized, and its antioxidant-related intestinal protective effects were evaluated in Drosophila melanogaster . HPGPC analysis showed a single symmetrical peak and indicated a relatively homogeneous fraction with a moderate molecular weight (Mw, 5.59 × 10 4 Da; ≈55.9 kDa). PMP-HPLC analysis revealed that SP0–1 was mainly composed of galactose, xylose, and arabinose (Gal/Xyl/Ara-rich; Gal:Xyl:Ara = 1:0.38:0.93). Methylation analysis combined with 1D/2D NMR spectroscopy supported the presence of major →4)- and →4,6)-linked Galp motifs, →5)- and →3,5)-linked Araf motifs, and terminal Xylp residues. The consecutive Gal–Gal arrangement, the residue adjacent to Gal O-6, the overall connection of the Ara/Xyl-containing region to the Gal-containing region, and the O-3 substituent of residue D remain unresolved. Functionally, SP0–1 exhibited concentration-dependent radical-scavenging activities against DPPH, ABTS• + , and hydroxyl radicals in vitro. In a Drosophila oxidative-stress model, dietary SP0–1 supplementation significantly improved survival and climbing performance, enhanced endogenous antioxidant enzyme activities, and reduced malondialdehyde levels. Moreover, SP0–1 markedly alleviated H 2 O 2 -induced intestinal oxidative injury, as evidenced by decreased reactive oxygen species accumulation and reduced intestinal epithelial cell death. These findings suggest that the structural characteristics of SP0–1, including its proposed Gal-rich linkage features and Ara/Xyl-containing residue motifs, may be associated with the observed antioxidant-related intestinal protection. Collectively, this study provides a structural and functional basis for further investigation of SP0–1 as a potential bioactive polysaccharide fraction.
危险品化学品经营许可证（带存储）