摘要
A triple-functional mPDA-SNO@5 h nanoplatform integrating PTT, NO therapy, and c -di-GMP pathway intervention is developed. • The nanoplatform achieves on-demand release of NO and 5 h under NIR or bacterial microenvironment stimuli. • Synergistic action of NO and 5 h enables both mature biofilm dispersal and sustained biofilm inhibition. • Combination of PTT and high-concentration NO achieves efficient eradication of biofilm-embedded persister bacteria. Drug resistance and recurrent chronic infection of Pseudomonas aeruginosa caused by biofilm formation are becoming increasingly serious. Particularly for infections caused by the clinically common hyper-biofilm phenotype mutant strain PAO1-Δ wspF . Traditional antibiotics alone are often insufficient to overcome mature biofilms and eradicate the bacteria within them, making it difficult to achieve the desired antibacterial effect. In this study, we designed an antibacterial nanoplatform mPDA-SNO@5 h, which can gradually release nitric oxide (NO) and cyclic diguanylate ( c -di-GMP) G-quadruplex inducer 5 h at physiological temperature, thereby interfering with the c -di-GMP signal pathway to eradicate and persistently inhibit the stubborn biofilm of PAO1-Δ wspF . Furthermore, under 808 nm near-infrared (NIR) light irradiation, the potent photothermal therapy (PTT) effect of mPDA-SNO@5 h, combined with the rapid release of a substantial amount of NO under light exposure, synergistically exerted exceptional antibacterial effects. In a mouse model of skin wound infection, this nanoplatform showed excellent antibacterial and wound-healing properties without inducing obvious toxic and side effects. The findings of our work provide a potential strategy for eradicating clinical biofilm-associated drug-resistant infections caused by Pseudomonas aeruginosa . Download: Download high-res image (77KB) Download: Download full-size image
危险品化学品经营许可证（带存储）