摘要
Probiotic therapy offers a promising strategy for modulating oral microbial balance and modulating host immunity, while its therapeutic efficacy may be hindered by poor mucosal retention and insufficient local persistence. Herein, a Lactobacillus rhamnosus GG (LGG) oral colonization tablet incorporating thiolated xanthan gum (L-XG) was developed to enhance mucosal adhesion and sustain localized probiotic delivery. The bilayer tablet consisted of an outer adhesive matrix and an inner probiotic reservoir, providing prolonged mucosal retention and sustained release of viable LGG. Comprehensive evaluation of physicochemical properties, critical quality attributes, and microbiological performance demonstrated satisfactory mucoadhesion and sustained release behavior. In a rat model of periodontitis, the LGG colonization tablet produced greater improvements in periodontal clinical indices, histological architecture, and local inflammatory responses than conventional LGG formulations. Importantly, 16S rRNA gene sequencing revealed treatment-associated modulation of the oral microbiota, characterized by increased microbial richness and diversity, reduced Porphyromonas abundance, and enhanced Lactobacillus abundance, with more pronounced changes observed in the colonization tablet group. Stability studies further demonstrated that the tablets retained their evaluated pharmaceutical and microbiological performance for up to 9 months under refrigerated storage (5 ± 3 °C, 60% ± 5% RH) when packaged in high-density polyethylene (HDPE) containers. Overall, the LGG oral colonization tablet provides a localized and sustained probiotic delivery platform that promotes favorable oral microbiota modulation and alleviates periodontal inflammation, supporting its further investigation as a localized probiotic delivery strategy for periodontal disease management. Download: Download high-res image (421KB) Download: Download full-size image
危险品化学品经营许可证（带存储）