APPLIED SURFACE SCIENCE
·
2023
·
IF 6.7
Liming Dai,Chenchen Fang,Fanglei Yao,Xiaoyuan Zhang,Xuefeng Xu,Shangling Han,Jingyao Deng,Junwu Zhu,Jingwen Sun
In-situ generated hydroxides and oxyhydroxides have been gradually recognized as the real active sites of metal organic frameworks (MOFs) towards the oxygen evolution reaction (OER). Nevertheless, the fine phase evolution process is still less of concern and elusive. Herein, we successfully construct serious of Ni-MOFs with differing thickness ranging from 1 nm to 270 nm through a competitive coordination strategy. Utilizing the operando resonance spectroscopy, an accurate phase inversion behavior of the Ni-MOFs has been witnessed during the OER, and demonstrated as a thickness-dependent process. Ultrathin architecture is identified as a positive factor to promote the exchange between MOFs ligands and applied electrolyte during the electrochemical test. Hence, after removing most of the internal ligands, 2D MOFs favor to be transformed as β-NiOOH, which is more active than the generated γ-NiOOH in bulky system. The density function theory (DFT) calculation alongside with the in-situ electrochemistry tests further affirm the β/γ-NiOOH transformation behavior. This work offers a new understanding of the electrocatalytic mechanism from the view point of phase evolution in 2D MOFs.
研究领域:
材料科学
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
Oncology Letters
·
2024
·Vol. 28
·期 3
·1-11
·
IF 2.5
Weimin Hu,Ling Cheng,Jian Zhao,Yonghui Wu,Ying Lin,Jun Yin
Transforming growth factor‑β (TGF‑β) signaling pathway serves a pivotal role in the pathogenesis of colorectal cancer (CRC). However, the specific molecular mechanisms by which the TGF‑β signaling pathway regulates CRC are still not fully understood. In the present study, metabolomics and transcriptomics were used to screen for key metabolites and regulatory genes most related to the regulation of the TGF‑β signaling pathway in CRC. Additionally, reverse transcription‑quantitative PCR, western blotting and Transwell assays were performed to assess the process of epithelial‑mesenchymal transition (EMT). Metabolomics analysis indicated that TGF‑β1 has an impact on purine metabolism, leading to an increase in the purine metabolite inosine. The increase of inosine is essential for facilitating EMT and cell migration in CRC cells. Furthermore, the integrated analysis of metabolomics and transcriptomics data revealed that TGF‑β1 induces the expression of laccase domain‑containing 1 (LACC1), an enzyme involved in the regulation of inosine. Knockdown of LACC1 resulted in a reduction of TGF‑β1‑induced alterations in inosine levels, EMT and cell migration in CRC cells. The results of the present study suggest that the TGF‑β signaling pathway is involved in the regulation of purine metabolism in CRC through the modulation of LACC1 expression. Furthermore, LACC1 appears to influence EMT and cell migration by elevating the levels of the purine metabolite inosine.
研究领域:
肿瘤学
·
分子生物学
·
癌症研究
·
细胞生物学
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产品关联
DOI
PubMed
高影响因子
BMC PLANT BIOLOGY
·
2022
·Vol. 22
·期 1
·1-15
·
IF 5.26
Wang Haitang,Zhou Xuesong,Liu Chuchu,Li Weixi,Guo Wangzhen
Background In eudicots, germination begins with water uptake by the quiescent dry seed and is greatly related to the permeability of micropyle enriched callose layers. Once imbibition starts, seeds undergo a cascade of physiological, biochemical, and molecular events to initiate cellular activities. However, the effects of callose on water uptake and following seed metabolic events during germination are largely unknown. Cotton ( Gossypium hirsutum ) is a eudicot plant with natural fiber and edible oil production for humans. Here, we addressed this question by examining the role of GhGLU19 , a gene encoding β-1,3-glucanase, in cotton seed germination. Results GhGLU19 belongs to subfamily B and was expressed predominately in imbibed seeds and early seedlings. Compared to wild type, GhGLU19 -suppressing and GhGLU19 -overexpressing transgenic cotton lines showed the higher and lower seed germination percentage, respectively. Callose was enriched more at inner integument (ii) than that in embryo and seed coat in cotton seeds. In GhGLU19 -suppressing lines, callose at ii of cotton seeds was greatly increased and brought about a prolonged water uptake process during imbibition. Both proteomic and transcriptomic analysis revealed that contrary to GhGLU19 -overexpressing lines, the glycolysis and pyruvate metabolism was decreased, and abscisic acid (ABA) biosynthesis related genes were downregulated in imbibed seeds of GhGLU19 -suppressing lines. Also, endogenous ABA was significantly decreased in GhGLU19 -suppressing line while increased in GhGLU19 -overexpressing line. Conclusions Our results demonstrate that suppression of GhGLU19 improves cotton seed germination via accumulating callose of inner integument, modulating glycolysis and pyruvate metabolism, and decreasing ABA biosynthesis. This study provides a potential way for improving germination percentage in cotton seed production, and other eudicot crops.
研究领域:
植物学
·
遗传学
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
DALTON TRANSACTIONS
·
2024
·Vol. 53
·期 29
·12090-12097
·
IF 3.5
Yingying Kong,Hongshan Wang,Wang Zhao,Qi Sun,Junjie Li,Shilie Pan
Hg-based compounds show abundant structural diversity and distinguished properties. Herein, a new phase transition compound CsHg2I5 was reported. The high-temperature phase β-CsHg2I5 with rare [Hg2I5] dimers was synthesized by the flux method at 573 K, and it shows a reversible phase transition at a low temperature of ∼100 K to form the low-temperature phase α-CsHg2I5. The two phases crystallize in the same P21/c space group, with different crystal structures. β-CsHg2I5 is composed of rare [Hg2I5] dimers and [CsI11] polyhedral units, while α-CsHg2I5 is composed of [Hg4I11] and [CsI10] units. The experimental band gap of β-CsHg2I5 was found to be 2.58 eV. Owing to the presence of [Hg2I5]∞ pseudo-layers, β-CsHg2I5 exhibits large optical anisotropy with a calculated birefringence of 0.132@1064 nm. Meanwhile, β-CsHg2I5 is a congruent compound and the congruent point is ∼481 K. Theoretical calculations indicate that the rare [Hg2I5] dimer is a nonlinear active unit, which can be used as a new fundamental building block for the design of advanced nonlinear optical materials. Moreover, a CsI–HgI2 pseudo-binary diagram was drawn. The results enrich the structural diversity of Hg-based halides and give some insights into the development of new functional materials based on rare [Hg2I5] dimers.
研究领域:
材料科学
·
晶体学
·
光学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Cells
·
2025
·Vol. 14
·期 6
·394
·
IF 5.2
Min Jiang,Jiaqi Hou,Qianqian Chai,Shihao Yin,Qian Liu
Pulmonary fibrosis due to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is the leading cause of death in patients with COVID-19. β-catenin, a key molecule in the Wnt/β-catenin signaling pathway, has been shown to be involved in the development of pulmonary fibrosis (e.g., idiopathic pulmonary fibrosis, silicosis). In this study, we developed a SARS-CoV-2-infected A549-hACE2 cell model to evaluate the efficacy of the A549-hACE2 monoclonal cell line against SARS-CoV-2 infection. The A549-hACE2 cells were then subjected to either knockdown or overexpression of the effector β-catenin, and the modified cells were subsequently infected with SARS-CoV-2. Additionally, we employed transcriptomics and raw letter analysis approaches to investigate other potential effects of β-catenin on SARS-CoV-2 infection. We successfully established a model of cellular fibrosis induced by SARS-CoV-2 infection in lung-derived cells. This model can be utilized to investigate the molecular biological mechanisms and cellular signaling pathways associated with virus-induced lung fibrosis. The results of our mechanistic studies indicate that β-catenin plays a significant role in lung fibrosis resulting from SARS-CoV-2 infection. Furthermore, the inhibition of β-catenin mitigated the accumulation of mesenchymal stroma in A549-hACE2 cells. Additionally, β-catenin knockdown was found to facilitate multi-pathway crosstalk following SARS-CoV-2 infection. The fact that β-catenin overexpression did not exacerbate cellular fibrosis may be attributed to the activation of PPP2R2B.
研究领域:
分子生物学
·
病毒学
·
医学
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产品关联
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2025
·
IF 5.6
Zhongya Zhang,Lijuan Zhang,Guangwu Wen,Yongzhao Hou,Nannan Zhu,Cheng Zhong,Jing Xue,Chuncheng Wei,Shuang Li
In this paper, polysilazane photocurable slurry with low viscosity and excellent rheological properties was prepared with β-Si 3 N 4 filler. Digital light processing (DLP) technique was utilised to form green bodies through optimized printing parameters. It was found that the introduction of β-Si 3 N 4 filler reduced the shrinkage and improved the mechanical properties of the polysilazane derived SiCN ceramics. The flexural strength of the resulted ceramics reached a maximum of 253.0 ± 15.1 MPa while 20 wt% of β-Si 3 N 4 powder was added, and the fracture toughness increased to 3.1 ± 0.3 MPa m 1/2 at 30 wt% of the β-Si 3 N 4 powder addition. The shape of the introduced β-Si 3 N 4 particles were believed to be faveriable for uniform dispersion in the printing slurry without agglomeration. In the following pyrolysis treament the β-Si 3 N 4 powders promoted the release of gaseous products so that maintained the structure integrety of the printed parts and the rod-like β-Si 3 N 4 particles enhanced the energy dissipation during ceramic fracture. In this study, silicon-based composite of β-Si 3 N 4 /SiCN ceramics with excellent mechanical properties were prepared by DLP additive manufacturing technology, which confirmed the feasibility and the great potential of photo-curing additive manufacturing technology in the manufacturing of SiCN ceramic matrix composites with complex structures.
研究领域:
材料科学
·
机械工程
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE RESEARCH
·
2014
·
IF 1.966
Yong-Fu Li,Yi-Ming Ha,Qin Guo,Qing-Peng Li
β-CD maleate (CDM) and β-CD itaconate (CDI) were prepared by esterification of β-cyclodextrin (β-CD) with maleic acid and itaconic acid using phosphate as a catalyst in a semi-dry process. The esterification of β-CD was carried out using [Itaconic acid ] or [Maleic acid ] 4 mol/mol of CD; M / L ratio 1:0.6; temperature 110 °C; [4-methoxyphenol] 2.5% amount of acid; reaction time 3.5 h. The esterification rates of CDM and CDI are 70.38% and 21.02%, respectively. We found that CDM and CDI were both monoesters. Here, we also established a new evaluation method for the rate of esterification.
研究领域:
有机化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2025
·
IF 5.6
Renping Cao,Zhiyuan Li,Jingheng Nie,Xiaochun Li,Lei Zhang,Ruirui Yang,Hui Ao,Jingjing Duan,Chenxing Liao
Near-infrared (NIR) luminescent materials based on Fe 3+ are growing interest in recent years because of the low-toxicity, high-efficiency, and adjustable emission. In this work, a broadband NIR emitting β-Mg 3 Ga 2 GeO 8 : Fe 3+ with [Ga/GeO 4 ] tetrahedron for the replacement of Fe 3+ ion is synthesized in air. X-ray diffraction patterns are used to confirm the crystal structure and phase purity. The excitation spectrum is observed in the region from 210 to 550 nm due to the O 2− - Fe 3+ charge transfer and the d – d electron transitions in 3 d 5 orbit of Fe 3+ . A broadband NIR emission of β-Mg 3 Ga 2 GeO 8 : Fe 3+ with full width at half maximum (∼108 nm) in the region from 610 to 880 nm is assigned to the transition 4 T 1 ( 4 G) → 6 A 1 ( 6 S) of Fe 3+ ion. Photoluminescence (PL) spectra under different excitation wavelengths, concentration dependent PL spectra, time-resolved PL spectra, temperature dependent PL spectra, and lifetimes of β-Mg 3 Ga 2 GeO 8 : Fe 3+ are investigated. We confirm the luminous center (Fe 3+ ), the optimal Fe 3+ concentration (0.8 mol%), and the good thermal stability of β-Mg 3 Ga 2 GeO 8 : Fe 3+ . The luminescence mechanism is analyzed theoretically by the Tanabe-Sugano and energy level diagrams of Fe 3+ in β-Mg 3 Ga 2 GeO 8 : Fe 3+ , and the concentration and thermal quenching mechanisms are explained. The research results will be conducive to the development of new Fe 3+ -doped luminescence materials.
研究领域:
材料科学
·
光学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2016
·Vol. 134
·期 2
·
IF 1.866
Zhi Chen,Ting Wang,Xiufeng Yan
Current cyclodextrin (CD) hydrogels have disadvantages, including a limited CD content, long reaction time, and complicated chemical reactions. To overcome these, a one-pot reaction was adopted, and a concept of a β-CD hydrogel cage was proposed. Hydroxyethyl cellulose (HEC) was used as the raw material with the adjustment of the stoichiometric relationship among the crosslinking agent of epichlorohydrin, alkali environment, and β-CD amount, and an elastic β-CD hydrogel cage system was established. After the β-CD was caged, the contents of active β-CD in hydrogel products reached 74.45 and 69.14 wt % by weight measurement and photometric titration, respectively. Furthermore, compared to the pure HEC hydrogel, the hydrogel mechanical strength improved obviously after the β-CD was caged, and we obtained a faster water swelling rate at the same time. Finally, a hydrophobic molecule of phenolphthalein (PP) was used to evaluated the activity of caged β-CD in the hydrogel. In addition, the absorption and release of PP by an elastic β-CD hydrogel cage at certain time intervals were also studied. The results demonstrate that the β-CD hydrogel cage was used as a functional molecule carrier to quickly absorb and sustain the release of PP. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017 , 134 , 44388.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
FASEB JOURNAL
·
2020
·Vol. 34
·期 3
·3583-3593
·
IF 4.966
Bu Yang,Shangfu Li,Zheng Chen,Feng Feng,Lei He,Bin Liu,Tianwei He,Xuan Wang,Ruiqiang Chen,Zihao Chen,Peigen Xie,Limin Rong
Background Amyloid β peptide (Aβ) is involved in osteoporosis, but the effects of Aβ on osteoblast and bone formation remain unclear. In this study, we investigated the effect of Aβ on bone formation. Methods An animal model of osteoporosis was established by ovariectomy in C57BL/6 mice. The mice received intraperitoneal injection of Aβ. The effect of Aβ on the osteogenic differentiation of human bone marrow stromal stem cells (hBMSCs) and differentiation of both pre-osteoblasts and pre-osteoclasts in a co-culture system were investigated. Results In the animal study, intraperitoneal injection of Aβ for 8 weeks promoted early and late osteogenic differentiation of hBMSCs. Aβ treatment significantly elevated osterix + (osteoblastic) cells but decreased TRAP + cells (osteoclasts) in the distal femur bone. In vitro study showed that Aβ treatment significantly enhanced matrix mineralization and osteogenic markers (Runx2 and osteocalcin). Aβ treatment activated Wnt/β-catenin signaling in hBMSCs. The effect of Aβ was blocked by DKK1 (a Wnt/β-catenin inhibitor) treatment. In the co-culture system, Aβ treatment significantly increased the ALP activities of MC3T3-E1 cells (pre-osteoblasts) but reduced the TRAP + RAW264.7 cells (pre-osteoclasts). Aβ treatment upregulated TCF1 and OPG proteins in MC3T3-E1 cells. Aβ treatment upregulated IκB-α but downregulated NFATc1protein in RAW264.7 cells. These effects were blocked by XAV-939 (a Wnt signaling antagonist), and then rescued by additional Wnt3a (a Wnt agonist). Conclusion Aβ treatment simultaneously promoted osteogenic differentiation via Wnt/β-catenin signaling, and inhibited osteoclasts differentiation via the OPG/RANKL/RANK system, suggesting Aβ is a positive regulator of osteoblast differentiation and bone formation.
研究领域:
生物学
·
医学
·
细胞生物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2021
·
IF 9.147
Yu-Xiao Wang,Ting Zhang,Jun-Yi Yin,Xiao-Jun Huang,Jun-Qiao Wang,Jie-Lun Hu,Shao-Ping Nie
A homogeneous β-glucan (JHMP-70) was obtained from Hypsizygus marmoreus by alkali extraction and ethanol precipitation, and had an apparent molecular weight of 394 kDa. Its fine structure was investigated by FT-IR, methylation combined with GC-MS and 1D and 2D NMR analysis. Results indicated JHMP-70 was a branched β-(1 → 6)-glucan substituted at O -3 by the non-reducing β-Glc p and/or β-(1 → 3)-linked β-Glc p side chains at a ratio of 1:1. SEM observation revealed that there were micro-spherical shapes intertwined with randomly distributed fibrous filaments. Rheological measurements showed that its flow behavior was highly dependent on the concentration. Newtonian behavior was evident at low concentration, whereas pseudoplastic behavior was observed at high concentration. Besides, the addition of salt ions (Na + or Ca 2+ ) significantly decreased the apparent viscosity of JHMP-70 solution. The low viscosity of JHMP-70 was probably attributed to its structural features, including main chain structure, branching degree and side chain length. This work can provide a basis theory to expand the application of the polysaccharides from H. marmoreus in food industry, because their lower viscosity and superior fluidity are desired in beverage products to impart stability against phase separation without compromising other sensorial properties.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
Catalysts
·
2023
·Vol. 13
·期 2
·267
·
IF 3.9
Jia Wu,Bao-Di Ma,Yi Xu
A novel and efficient one-pot three-enzyme cascade method for the synthesis of β-alanine from maleic acid was developed. Two recombinantE. colistrains were constructed. TheE. coli(MaiA-AspA) co-expressing maleiccis-trans isomerase (MaiA) and L-aspartase (AspA) catalyzed the biotransformation of maleic acid to L-aspartate via fumaric acid, andE. coli(ADC) expressing L-aspartate-α-decarboxylase (ADC) catalyzed the bioconversion of L-aspartate to β-alanine. After systematic optimization of reaction conditions for each strain, the whole cells of two strains were combined for one-pot synthesis of β-alanine. It was found that the ratio of the two kinds of cells as well as the cell amount play critical roles in the reaction rate and yield of β-alanine. Adding two kinds of cells in one-pot at the beginning of the reaction was better than adding step by step. Under optimal conditions, the concentration of β-alanine reached 751 mM after a 9 h reaction, corresponding to a 93.9% yield and 178 g/L/d space-time yield. The developed new route showed application potential for green and efficient biosynthesis of β-alanine from a cheap substrate by tandem biocatalysts.Keywords:β-alanine;cascade reaction;biotransformation;one-pot
研究领域:
生物化学
·
合成生物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）