NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 7
·3361-3367
·
IF 3.3
Shiqi Cheng,Jin Yang,Shangying Qin,Li Huang,Yuanjin Xu,Yilin Wang
Three-dimensional reduced graphene oxide (3DrGO) and a flower-like zinc oxide (ZnO) crystal were prepared using a hydrothermal method. A stable dispersion of the 3DrGO–ZnO composite was formed by sonication mixing, and was further applied to modify a glassy carbon electrode for direct trace detection of ascorbic acid (AA), dopamine (DA) and uric acid (UA). The structure, composition and morphology of the as-prepared 3DrGO–ZnO composite were characterized using X-ray diffraction, X-ray photoelectron spectroscopy and scanning electron microscopy, respectively. Compared with bare, 3DrGO and ZnO electrodes, the 3DrGO–ZnO electrode exhibited an obvious electrocatalytic enhancement effect. Furthermore, the 3DrGO–ZnO electrode could well distinguish and enhance the differential pulse voltammetry signals of AA, DA and UA. Under the selected conditions, the peak current responses to AA, DA and UA were linear in the range of 100–2500 μmol L−1, 2.00–35.0 μmol L−1, and 1.00–150 μmol L−1, respectively. To verify its possible application, the constructed modified electrode was successfully used for the analysis of real samples with satisfactory results.
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Frontiers in Pharmacology
·
2023
·
IF 5.6
Yang Yang,Cuiting Lin,Qiang Zheng,Leqi Zhang,Yongmei Li,Qinghua Huang,Ting Wu,Zean Zhao,Lu Li,Jian Luo,Yanqing Jiang,Qun Zhang,Xing Wang,Chenglai Xia,Jianxin Pang
Hyperuricemia (HUA) is associated with left ventricular remodeling (LVR) and thereby causes the initiation and development of a large number of cardiovascular diseases. LVR is typically accompanied by cardiomyocyte energy metabolic disorder. The energy supply of cardiomyocytes is provided by glucose and fatty acid (FA) metabolism. Currently, the effect of HUA on cardiomyocytic FA metabolism is unclear. In this study, we demonstrate that UA-induced cardiomyocyte injury is associated with cytoplasmic lipid deposition, which can be ameliorated by the FA metabolism-promoting drug L-carnitine (LC). UA suppresses carnitine palmitoyl transferase 1B (CPT1B), thereby inhibiting FA transport into the mitochondrial inner matrix for elimination. LC intervention can ameliorate HUA-associated left ventricular anterior wall thickening in mice. This study showed that FA transport dysfunction plays is a critical mechanism in both cardiomyocytic injury and HUA-associated LVR and promoting cytoplasmic FA transportation through pharmacological treatment by LC is a valid strategy to attenuate HUA-associated LVR.
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BIOSENSORS & BIOELECTRONICS
·
2023
·
IF 12.6
Wanru Li,Haiyan Wei,Nianlu Li,Shunyu Li,Yaru Liu,Ranran Liu,Wei Zou,Peishan Hu,Zhihu Zhang,Cuijuan Wang
Rapid detection of diquat (DQ) is essential in clinical diagnosis and rescue. Here, we developed a fast, simple-yet-practical detection strategy for the reliable identification and quantification of DQ in biological fluids. Based on surface-enhanced Raman spectroscopy (SERS), point-of-care detection was realized under the acidic condition with gold nanoparticles as the substrate. Under optimal experimental conditions, the detection limits of the strategy were 17.5 ppb and 1.99 ppm in human urine and gastric juice, respectively. High specificity and selectivity of the SERS strategy were demonstrated using common pesticides and coexisting biological substances. The method was also used to detect biofluids from 5 patients and urine samples from 10 healthy volunteers . The results were in high agreement with spectrophotometric and clinical liquid chromatography-mass spectrometry methods. The volume of urine samples required for this technique is merely 20 μL, and no preparation of the samples is required. Compared to traditional methods used in clinical settings, SERS-based methods are capable of real-time measurements that accurately provide rapid detection and response in non-laboratory settings, with great potential for on-site and point-of-care testing.
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ACS Sensors
·
2023
·
IF 8.9
Zhuanzhuan Shi,Fang Shi,Yunpeng Li,Xiaoshuai Wu,Zhengyang Liu,Liang Liu,Qianqian Fu,Chang Ming Li,Chunxian Guo
Cell’s activities are highly dependent on signal molecules, of which reactive oxygen species of the superoxide anion (O2•–) and hydrogen peroxide (H2O2) are important ones that always work together to regulate biological processes such as apoptosis and oxidative stress. It is of significance to realize simultaneous qualification of O2•– and H2O2 but it still faces challenges particularly in live-cell assay with a complex environment. We report the design of a bifunctional sensing material by interfacially regulating ZIF-67 on bacteria Shewanella putrefaciens to generate cobalt nanoparticles/nitrogen-doped porous carbon nanorods (Co/N-doped CNRs) and its sensing chip for qualifying cell-released O2•– and H2O2. Co/N-doped CNRs exhibit unique properties including porous structure for significantly increased reaction surface area and coordinating Co nanoparticles for rich active sites. The bifunctional Co/N-doped CNRs is used to fabricate the electrochemical sensing chip, which achieves a fast response time (0.5 s for O2•–, 1.9 s for H2O2), a low detection limit (0.69 nM for O2•–, 2.25 μM for H2O2), and a remarkably high sensitivity (792.30 μA·μM–1·cm–2 for O2•–, 153.91 μA·mM–1·cm–2 for H2O2), among the best of reported bifunctional nanozymes.
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ANALYST
·
2022
·Vol. 148
·期 2
·344-353
·
IF 5.227
Yufeng Sun,Shengwen Wang,Fangfang Wang,Hao Zhang,Weiya Huang,Aiguo Wu,Yujie Zhang
Urinary potassium is an important parameter in clinical health diagnosis. Rapid and convenient detection of potassium ions (K+) in urine is essential for personal healthcare and health management. Here, crown ether (4-aminodibenzo-18-crown-6, ADC) modified silver nanoparticles (ADC-Ag NPs) were successfully prepared for one-step rapid colorimetric detection of urinary potassium. The detection mechanism is as follows: due to the matching sizes of the diameter of K+ and the cavity in crown ether 6, K+ is encapsulated between the cavities of two crown ethers, resulting in the clumping of ADC-Ag NPs and the color of the solution being altered. The colorimetric detection method has a fast response and is completed within 20 minutes. It also shows good selectivity and interference immunity. The lowest detectable concentration is 20 μM with the naked eye and 2.16 μM for UV-vis absorption spectroscopy. A good linear relationship (R2 = 0.9931) between the absorption intensity ratio and K+ concentration (0–100 μM) indicates that this colorimetric probe can be used to detect K+. The method was also applied for quantitative analysis of K+ in real urine samples with recovery between 116 and 120%.
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TALANTA
·
2022
·
IF 6.556
Chiyu Ma,Liubing Kong,Xianyou Sun,Yanchi Zhang,Xinyi Wang,Xinwei Wei,Hao Wan,Ping Wang
Uric acid (UA) is the final product of purine metabolism. A high concentration of UA in body fluid may lead to kidney stones, gout, and some cardiovascular diseases. Therefore, the non-invasive daily monitoring of UA is of great significance for both hyperuricemia patients and fit people. However, most of the current detection methods for UA are enzyme-dependent which limits the application scenarios and lacks portable instruments for on-site detection, including optics and electrochemistry . In this work, an enzyme-free and wide-range colorimetric sensor for UA and H 2 O 2 detection was developed based on a mercaptosuccinic acid (MSA)-modified Cu nanoparticles (CuNPs). Under the action of UA or H 2 O 2 , with the cleavage of MSAs on the CuNPs surface, small Cu particles are further aggregated into larger particles with a lightning violet color. With the employment of the multi-channel handheld automatic photometer (MHAP), the concentration of UA and H 2 O 2 can be determined on-site according to the absorbance measurement by the photodiodes. The linear range of UA was 5 μM-4.5 mM with the limit of detection (LOD) of 3.7 μM, while the linear range of H 2 O 2 was 5 mM–500 mM and 5 μM-5 mM with the LOD of 4.3 μM. This approach has been applied to the detection of UA in human urine, providing more possibilities for non-invasive home health monitoring, community medical diagnosis, and broader prospects of on-site disease detection.
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ACS Nano
·
2022
·
IF 18.027
Ruijia Huang,Xiaoqing Cai,Jihui Du,Jie Lian,Ping Hui,Minxuan Gu,Feng Li,Jidong Wang,Wenwen Chen
Delayed use of appropriate antibiotics for superbugs, particularly for extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pn), has caused extensive morbidity and mortality worldwide. Therefore, rapid and on-site antimicrobial susceptibility testing (AST) is urgently required. Unfortunately, currently, no phenotypic AST can realize a sample-to-answer result within 2 h directly from a clinical sample and without using laboratory equipment or customized devices. Inspired by observing that E. coli and K. pn can rapidly catalyze H2O2, we developed a plasmonic nanosensor that responds to the proliferation of bacteria for realizing rapid AST. The results can be determined with the naked eye, digitized using a smartphone, and validated using ultraviolet–visible spectrometry. Our assay achieved superb area under the curves of 0.9752 and 1 in a receiver operating characteristic analysis directly obtained from uncultured clinical urine samples infected by E. coli and K. pn, respectively. The entire process from sample collection to analysis takes 100 min for E. coli and 85 min for K. pn detection. Our platform provides a practical approach for performing on-site AST in clinics to improve the survival of patients. It releases the burden of superbugs and avoids the abuse of antibiotics.
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Materials Chemistry Frontiers
·
2023
·Vol. 7
·期 8
·1624-1632
·
IF 7
Kangkang Wang,Zhexue Chen,Yueqi Li,Yong Zhang
Semiconductor quantum dots (QDs) are of great interest for a wide range of applications. Herein, we report the top-down production of CdSe QDs with a super small size (about 3 nm) and high yield (14%). The as-produced QDs exhibit different optical properties from those of colloidal QDs. This is probably due to the generation of numerous bare edges during the process of silica-assisted ball-milling. The CdSe QD-based substrate could serve as a surface-enhanced Raman scattering (SERS) substrate for the ultrasensitive recognition of probe molecules at trace levels, which are mainly attributed to the special edge–edge interactions and charge transfer (CT) enhancement mechanism. The substrate exhibits good reproducibility, uniformity, and high enhancement factor (5.05 × 106) with an expansion of the ultra-low limit of detection (LOD) to 10−10 M. It has also been successfully used in the reliable, quantitative, and label-free detection of uric acid (UA) at a low concentration (1 μM), which facilitates the early prevention of gout disease. This QD-based platform not only confirms the feasibility of SERS analysis but also provides ideas for the early detection of residues.
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Materials
·
2023
·Vol. 16
·期 3
·1261
·
IF 3.4
Qian Wang,Yuzhe Wang,Guiyong Xiao,Xinde Zhu
In this work, the facile fabrication of Co3O4particles/reduced graphene oxide (Co3O4/rGO) composites on Indium tin oxide (ITO) slide was achieved by an electrophoretic deposition and annealing process. The deposition time and ratio of the precursors were optimized. Structural characterization and chemical composition investigation indicated successful loading of Co3O4particles on graphene sheets. When applied as a non-enzymatic H2O2sensor, Co3O4/rGO showed significant electrocatalytic activity, with a wide linear range (0.1–19.5 mM) and high sensitivity (0.2247 mA mM−1cm−2). The good anti-interference ability, reproducibility, and long-term stability of the constructed sensor were also presented. The application of Co3O4/rGO in real sample analysis was evaluated in human urine sample with satisfactory results, indicating the feasibility of the sensor in physiological and medical applications.
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SENSORS
·
2023
·Vol. 23
·期 2
·695
·
IF 3.9
Zhipeng Yu,Huan Wu,Zhongshuang Xu,Zhimao Yang,Jian Lv,Chuncai Kong
Designing highly active material to fabricate a high-performance noninvasive wearable glucose sensor was of great importance for diabetes monitoring. In this work, we developed CuxO nanoflakes (NFs)/Cu nanoparticles (NPs) nanocomposites to serve as the sensing materials for noninvasive sweat-based wearable glucose sensors. We involve CuCl2to enhance the oxidation of Cu NPs to generate Cu2O/CuO NFs on the surface. Due to more active sites endowed by the CuxO NFs, the as-prepared sample exhibited high sensitivity (779 μA mM−1cm−2) for noninvasive wearable sweat sensing. Combined with a low detection limit (79.1 nM), high selectivity and the durability of bending and twisting, the CuxO NFs/Cu NPs-based sensor can detect the glucose level change of sweat in daily life. Such a high-performance wearable sensor fabricated by a convenient method provides a facile way to design copper oxide nanomaterials for noninvasive wearable glucose sensors.Keywords:noninvasive wearable sensing;metal oxide;sweat glucose detection
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Nanomaterials
·
2022
·Vol. 12
·期 23
·4306
·
IF 5.719
Huifang Wu,Mengqi Xu,Yubing Chen,Haoliang Zhang,Yongjun Shen,Yanfeng Tang
The specific detection of oxytetracycline (OTC) residues is significant for food safety and environmental monitoring. However, rapid specific determination of OTC from various tetracyclines is still challenging due to their similar chemical structures. Here, nitrogen-doped carbon dots (NCDs) with excitation and pH-dependent optical properties and a high-fluorescence quantum yield were successfully synthesized, which were directly employed to fabricate a dual-response fluorescence probe by self-assembly with Eu3+(NCDs/Eu3+) for the ratiometric determination of OTC. The addition of OTC into the probe greatly enhances the characteristic emission of Eu3+due to the “antenna effect”, and the incorporation of NCDs into the probe further improves the Eu3+fluorescence by remarkably weakening the quenching effect caused by H2O molecules and efficiently shortening the distance of energy transfer from OTC to Eu3+. Meanwhile, the fluorescence of NCDs apparently decreases due to aggregation-caused quenching. The results demonstrate that a ratiometric detection of OTC (0.1–25 µM) with a detection limit of 29 nM based on the double response signals is achieved. Additionally, visual semi-quantitative assay of OTC can be realized with the naked eye under a 365 nm UV lamp according to the fluorescence color change of the as-fabricated probe. This probe exhibits acceptable specificity and anti-interference for OTC assay, holding promise for the fast detection of OTC in real water and milk samples.Keywords:oxytetracycline;nitrogen-doped carbon dots;lanthanide;ratiometric;visual analysis
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MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Yong Zhao,Yixuan Xia,Juerui Zhang,Hongde Liu,Yinhui Yi,Gangbing Zhu
In this work, based on the unique self-adsorption and reduction capacities of Ti 3 C 2 T x MXenes toward silver ion, silver nanoparticles (Ag NPs) as reference probe are synthesized and immobilized on the surface of Ti 3 C 2 T x nanoribbons (Ti 3 C 2 T x R) to form Ag-Ti 3 C 2 T x R nanohybrid with a simple stirring-method, and carbon nanotubes (CNTs) are introduced to conjugate Ag-Ti 3 C 2 T x R for inhibiting their aggregation and improving further the sensing performance. Via selecting paracetamol (PA) as a model analyte, a ratiometric voltammetric sensing strategy is proposed by using Ag-Ti 3 C 2 T x R/CNTs as electrode materials on the basic of the collaborative advantages of Ag NPs, Ti 3 C 2 T x R and CNTs: in the absence of PA, Ag-Ti 3 C 2 T x R/CNTs modified electrode displays only an electrochemical signal from the oxidation of Ag 0 ; while in the presence of PA, a varied signal of PA along with its concentration can be observed coupled with a quite stable oxidation signal of Ag 0 . Based on this principle, a highly sensitive and reliable ratiometric sensing platform is designed for PA detection by recording the ratio of signals between PA and Ag 0 . It’s expected that this work exploits a new research field in electrochemical sensors by combining the synthetic advantages of MXenes and metal elements.
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