INDUSTRIAL CROPS AND PRODUCTS
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2023
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IF 5.9
Jingwen Sun,Yu Xie,Zhiyuan Chen,Yipin Fan,Yunfeng Liu,Qi Gao,Jianqiang Li,Jingwen Bai,Yu Yang
Magnolia officinalis root extract was obtained by high-pressure-assisted extraction, and the yield of honokiol (HNK) was 86.97 ± 1.05 mg/g dry power. The purified extracts and HNK inhibited the growth of methicillin-resistant Staphylococcus aureus (MRSA). Assays for alkaline phosphatases , proteins, nucleic acids leakage, and electrical conductivity, along with scanning and transmission electron microscopy measurements, confirmed the damaging effects of HNK on the cell wall and membrane of MRSA. Intake of sugar, protein production, molecular docking , and dynamic simulations revealed that HNK interfered with the transport of mannose-based carbohydrates by binding to VAL196, ILE291, ALA293, PRO294, and MET214 on the mannose transporter (ManP) in the phosphotransferase system, and thereby inhibited the carbohydrate metabolism of MRSA. These results indicated that HNK prevented MRSA from using carbohydrates by cutting off its nutritional process, and ultimately caused bacterial rupture and lysis. Therefore, ManP may be a potential target for HNK to combat MRSA infections.
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CARBOHYDRATE POLYMERS
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2023
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IF 11.2
Shuwei Yuan,Jiahui Wang,Xiang Li,Xun Zhu,Zhenqing Zhang,Duxin Li
The lotus ( Nelumbo nucifera Gaertn.) is the largest aquatic vegetable in Asia. The lotus seedpod (LS) is an inedible part of the mature flower receptacle of the lotus plant. However, the polysaccharide isolated from the receptacle has been less studied. The purification of LS resulted in two polysaccharides (LSP-1 and LSP-2). Both polysaccharides were found to be medium-sized HG pectin, with a Mw of 74 kDa. Their structures were elucidated via GC–MS and NMR spectrum and proposed as the repeating sugar units of GalA connected via α-1,4-glycosidic linkage, with LSP-1 having a higher degree of esterification . They have certain content of antioxidant and immunomodulatory activities. The esterification of HG pectin would have an adverse effect on these activities. Furthermore, the degradation pattern and kinetics of LSPs by pectinase conformed to the Michaelis-Menten model. There is a large amount of LS, resulting from the by-product of locus seed production, and thus a promising source for the isolation of the polysaccharide. The findings of the structure, bioactivities, and degradation property provide the chemical basis for their applications in the food and pharmaceutical industries .
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PubMed
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JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
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2023
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IF 10.9
Na Liang,Zixu Yin,Jingyun Guo,Wanying Fang,Qi Wang,Ge Tian,Dong Zhang,Huijuan Yue,Shouhua Feng
Exploring new strategies to incorporate the concepts of green chemistry and defect engineering into new architectures to address the problem of severe electromagnetic wave (EMW) pollution is challenging. In this work, by using a by-product of catalytic conversion of biomass as a carbon source, we prepare two-dimensional (2D) Co 9 S 8 -coated humins-derived carbon nanomaterials with a yolk-shell structure. The EMW attenuation ability is finally optimized by controlling the morphology and defects of the composite material through the pyrolysis temperature. As expected, with the synergy of conductive loss/interfacial polarization and defects regulating, the composite material achieves a reflection loss (RL) value of −51.4 dB in Ku-band and the effective absorption bandwidth (EAB) can be as high as 5.92 GHz (12.08–18.00 GHz) at 1.8 mm ultra-thin coating thickness. A strong absorption capacity in the low-frequency C-band (6.4 GHz) reaches RL value of −49.9 dB and a thickness of 3.50 mm, which grants it the characteristic of convertible microwave absorption from high frequency to low frequency. Taking account of the facile synthesis and tunable absorption properties, the encouraging findings shed light on exploring waste-turned-microwave absorbents with promising practical applications.
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FITOTERAPIA
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2023
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IF 3.4
Saijuan Li,Fangge Wu,Pengcheng Gao,Can Jin,Yuyong Wang,Wenfeng Liao,Kan Ding
The accumulation of amyloid β (Aβ) containing senile plaques is one of the key histopathological hallmarks of Alzheimer's disease (AD). Increasing evidences demonstrated the important role of autophagy in Aβ clearance. Recent studies implied that extracts from Semiaquilegia adoxoides (DC.) Makino could ameliorate the memory of D-galactose induced aging mice. However, the bioactive substance and underlying mechanism remains unknown. Thus, the present study sought to explore the effects of a novel homogenous peptidoglycane on Aβ 42 secretion and the underlying mechanism. Briefly, we extracted a novel peptidoglycan named SA02C using hot water extraction and alcohol precipitation with the Mw of 13.72 kDa. SA02C contains 73.33% carbohydrate and 27.83% protein. The structure characterization revealed that its glycan part might mainly composed of galacturonic acid with minor rhamnose in backbone, and branched with glucose, galactose, arabinose, xylose and galacturonic acid. The protein or peptide moiety in SA02C was bonded to the polysaccharide via threonine. Bioactivities test showed that SA02C could reduce Aβ 42 production in a dose dependent manner with no obvious cytotoxicity. Mechanism study demonstrated that SA02C could modulate APP processing by upregulating the expression of ADAM10, sAPPα and downregulating BACE1, sAPPβ. Furthermore, SA02C also could stimulate autophagy by promoting the expression of the markers of autophagy such as LC3B and ATG5, resulting in the promotion of Aβ 42 phagocytosis.
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CATALYSIS COMMUNICATIONS
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2023
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IF 3.7
Dan Luo,Qin Wang,Jiansu Ran,Ruixue Yangcheng,Yuntong Cui,Shuang Luo,Jianjian Wang
A series of Zr-based catalysts were prepared using various commercial and cheap organic acids as the coordination agents, and these as-synthesized samples exhibited superior activities to produce lactic acid (LaA) from carbohydrates. Using humic acid (HA) as a typical coordination agent, LaA yield was up to 76% over the resultant Zr-HA, outperforming the most recent reported works. Deeper studies revealed that Zr-HA possessed both acid sites and base sites, and these dual active sites can simultaneously activate xylose, resulting in high yield production of LaA. Besides, Zr-HA also showed excellent reusability and can be reused at least five times.
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CATALYSIS LETTERS
·
2023
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IF 2.8
Zhu Peng,Han Chunyang,Xia Haian
Catalytic hydrogenolysis of xylose to ethylene glycol (EG) and 1,2-propanediol (1,2-PG) is an important way for the high-value valorization of biomass. Herein, Ni-W/CeO 2 catalysts prepared by incipient-wetness impregnation method were used for the hydrogenolysis of xylose to EG and 1,2-PG. Several characterizations such as X-ray diffraction (XRD), Transmission electron microscopy (TEM) and X-ray photoelectronic spectroscopy (XPS) were used to analyze the physicochemical properties of the catalysts. The effects of different Ni/W ratios and reaction conditions on the hydrogenolysis performance of the catalyst were investigated. The doping of W promoted the selective cleavage of C–C and C-O bonds of xylose, thus improved the yield of EG 1,2-PG. The doping of W can form interactions with Ni and increase the oxygen vacancy concentration of CeO 2 , which have a significant impact on the catalyst activity. When the Ni: W ratio is around 1:1, the catalyst has the best activity, and the total yield of diols can reach about 43.2%.. When xylan was used as the substrate, the total yield of diols can reach to 58.7%. The possible reaction mechanism of xylose hydrogenolysis was also discussed. This work developed a new catalyst system for the directional conversion of xylose to EG and 1,2-PG, which provided a new idea for the design of catalyst to converting lignocellulose, especially carbohydrate into high-value chemicals. Graphical Abstract Ni-W/CeO 2 with different Ni/W ratio are efficient catalysts for hydrogenolysis of xylose to EG and 1,2-PG.
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CHEMICAL PAPERS
·
2023
·Vol. 77
·期 10
·5999-6006
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IF 2.2
Yuan Yifan,He Fuduo,Li Xue,Xu Dehua,Yan Zhengjuan,Wang Xinlong
Corncob has a high yield and contains a large number of cellulose substances. Wet phosphoric acid hydrolysis of corncob is of great significance to the utilization of organic waste, and provides theoretical support for the development of sugar-containing synergistic phosphorus fertilizers. In this study, the kinetics of hydrolysis of corncob by wet process phosphoric acid was investigated. The time evolution of total sugar, xylose and furfural formation during a 12-h hydrolysis was simulated by Saeman and biphasic models within differential acid concentrations and temperatures. The formations of both total sugar and furfural coincide a first-order irreversible reaction, which was not applicable to the Saeman model nor the biphasic model. Meanwhile, xylose formation was more suitable for the Saeman model. The optimal conditions for xylose are: a temperature of 100 °C, a reaction time of 720 min, an acid concentration of 55.6% (w/w). The xylose yield of corncobs was 0.15 g/g in the optimal conditions. Graphical abstract
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FOOD RESEARCH INTERNATIONAL
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2023
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IF 8.1
Yujiao Sun,Xushuang Yuan,Zhaojun Luo,Yungang Cao,Shuai Liu,Yang Liu
Liquid-state fermentation (LSF) of tea leaves is a promising way to obtain tea-based nutraceutical products rich in various bioactive compounds. In the study, the changes of bioactive compounds, tea pigments and complex metabolites from LSF of primary dark tea, green tea and white tea infusions with Aspergillus cristatus were determined. Chemical analyses revealed that soluble sugars, monosaccharide composition, total polyphenols, total flavonoids, free amino acids, soluble proteins and tea pigments were changed in different ways. An untargeted metabolomic analysis and ribonucleic acid sequencing (RNA-seq) based transcriptomic analysis were performed to investigate the metabolic differentiation and clarify the key differentially expressed genes (DEGs, fold change >2 and p < 0.05), showing that amino acid metabolism, carbohydrate metabolism and lipid metabolism were the most enriched pathways during A. cristatus fermentation of primary dark tea, green tea and white tea infusions. In addition, glycerophospholipid metabolism, linoleic acid metabolism and phenylalanine metabolism were greatly accumulated in the fermentation of primary dark tea and white tea infusions; Pyruvate metabolism, glycolysis/gluconeogenesis, fatty acid degradation, tyrosine metabolism, phenylalanine, tyrosine and tryptophan biosynthesis and valine and leucine, isoleucine degradation were greatly accumulated in the fermentation of primary dark tea and green tea infusions; Starch and sucrose metabolism was greatly accumulated in the fermentation of green tea and white tea infusions; Galactose metabolism was significantly enhanced in the fermentation of primary dark tea infusion; Amino sugar and nucleotide sugar metabolism, sphingolipid metabolism and alanine, aspartate and glutamate metabolism were significantly enhanced in the fermentation of green tea infusion. Besides, some other pathways involving aminobenzoate degradation, biosynthesis of cofactors, pyrimidine metabolism, benzoxazinoid biosynthesis and phenazine biosynthesis, tropane, piperidine and pyridine alkaloid biosynthesis and flavone and flavonol biosynthesis also differed from each other. These findings support that A. cristatus plays a vital role in the biochemical and genetic regulation of metabolite profile, and could be considered a potential prospect for better use of A. cristatus on different kinds of tea materials.
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JOURNAL OF MOLECULAR LIQUIDS
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2023
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IF 6
Ruoyu Du,Weiwei Guo,Yu Shen,Jinhang Dai,Haidong Zhang,Ming Fu,Xingmin Wang
The DNS assay has been already a universally method for the in situ determination of reducing sugars (RS) in aqueous phase solvents. Today, “greener biorefineries” require in situ measurement of reducing sugars in non-aqueous solvents such as natural deep eutectic solvents (NADESs). Therefore, that DNS assay cannot be performed successfully in such eutectic phase solvent becomes a puzzle that must be solved. In this work, an improved DNS method, which we call the DNS'' method, was established and implemented successfully in hydrophilic eutectic phase solvent systems ( hi NADES). The tickets to the success of DNS'' method are the inserted two essential steps, water-adding disintegration and pH alkalization. Then we tested two excellent RS solvents (ChCl_Gly and ChCl_LA) from six synthetic ChCl-based hi NADESs. The optimal disintegration conditions for assaying RS in ChCl_LA/NaOH (ChCl_Gly/H 2 O) were volume ratio 3: 1 (3: 1), molar concentration 2.0 mol/L (0 mol/L). Thereafter, we drew the standard curves of seven RSs in ChCl_Gly and ChCl_LA, which are glucose, xylose, fructose, mannose, fucose, rhamnose, and maltose. We determined that HMF interferes with DNS assay in hi NADES. The principles and standard procedures of DNS'' method were also investigated. Finally, the enzymatic saccharification of microcrystalline cellulose (MCC) by two commercial Trichoderma reesei cellulases (Celluclast® 1.5 L and Cellic® CTec2) in neat hi NADES was tracked using the DNS'' method.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2023
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IF 8.6
Yuxiao Hu,Xueying Chen,Zhijian Tan
Ammonium sulfate and t -butanol are widely used in conventional three-phase partitioning (TPP); however, their use is not eco-friendly. In this study, a novel TPP was developed using pH-responsive deep eutectic solvents (DESs) and sugars as substitutes for t -butanol and ammonium sulfate, respectively. This TPP was further used for the separation and purification of radish ( Raphanus sativus L.) peroxidase (POD). DES-3 (dodecanoic acid: decanoic acid = 1:1) and sucrose were selected as optimal phase-forming components. Single-factor experiments were performed to investigate the factors influencing the TPP process, and three major factors (sucrose concentration, extraction time, and extraction temperature) were optimized by response surface methodology (RSM). Under the optimized conditions, the POD recovery and purification fold reached 101.66% and 11.56, respectively. Cyclic experiments confirmed the retrievability of DESs. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) confirmed that the novel TPP had better purification efficiency than the traditional TPP. The method proposed in this study is more efficient than conventional TPP, and it does not require the use of organic solvents and high concentration salt solutions. Therefore, this method is promising in the application of TPP.
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ChemistrySelect
·
2023
·Vol. 8
·期 16
·e202300902
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IF 2.1
Panli Liu,Shengbin Shi,Ruiping Wei,Lijing Gao,Jin Zhang,Guomin Xiao
Graphical P-Zr-SBA-15 catalyst with bifunctional acidity was synthesized by introducing Zr 4+ into the mesoporous material SBA-15 structure for intra skeleton modification, and then impregnated with phosphoric acid solution to modify the outer skeleton. The catalyst exhibits excellent catalytic activity, good thermal stability, and recoverability in the preparation of furfural. A series of bifunctional P-Zr-SBA-15 catalysts with Lewis acid and Brønsted acid sites were prepared by modifying mesoporous material SBA-15 with Zr 4+ and phosphate group, which were used in the conversion of xylose and xylan into furfural. The structure and acidic characteristics of the catalyst were analyzed by X-ray diffraction, Fourier transform infrared spectroscopy, N 2 adsorption desorption, scanning/transmission electron microscopy, X-ray photoelectron spectroscopy, thermogravimetric analysis, NH 3 temperature programmed desorption, and pyridine adsorption infrared spectroscopy. The effects of different process parameters on the catalytic performance of the catalyst were studied. when using the xylose and xylan as substrates, the furfural yield were 80.3 % and 68.8 % under the optimal conditions in the H 2 O (NaCl) /toluene (3/3 mL) biphasic solvent system, respectively, and 52.5 % furfural yield was obtained using xylose as substrate in aqueous system. In addition, the catalyst has good thermal stability and easy recovery in the recycling experiment.
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Journal of Environmental Chemical Engineering
·
2023
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IF 7.7
Yanting Lv,Weiyong Shao,Yuling Kong,Nanhua Li,Xinrui Huang,Zhihao Tang,Murong Gong,Lizi Li,Weiqi Wei
Photocatalytic reforming of biomass has a broad prospects in the green and efficient production of high-value chemicals, which was in line with the sustainable development. Herein, we proposed a new photocatalytic system to produce lactic acid from xylose by using boron doping carbon nitride supported cuprous oxide (g-C 3 N 4 -B-Cu 2 O) under a weak alkaline system. The UV–vis and photoluminescence (PL) characterization showed that the introduction of boron and Cu 2 O into C 3 N 4 not only improved the catalyst UV–visible light absorption range, but promote the separation ability of photogenerated electron holes , which was beneficial for lactic acid forming. The poisoning experiments indicated that the free radicals such as 1 O 2 ,·OH,·O 2- and h + were the key active substances in the process of xylose photocatalytic reforming into lactic acid, and with the sacrificial agents such as benzoquinone (BQ), ethylene diamine tetraacetic acid (EDTA), isopropyl (IPA), and tryptophan (Trp) use, the yield of lactic acid significantly reduced. Remarkably, the maximum lactic acid yield of this work was 91.2 %, and it was obtained at 60 ℃ for 120 min with 99.3 % xylose conversion. More importantly, the g-C 3 N 4 -B-Cu 2 O photocatalyst exhibited the satisfied stability, and can be reused at least for nine times with the lactic acid yield loss less than 2.3 %. Finally, the plausible reaction pathway for xylose photocatalytic reforming to lactic acid over g-C 3 N 4 -B-Cu 2 O were also investigated in this work.
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