FOOD CHEMISTRY
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2022
·
IF 9.231
Zhengzheng Zhao,Zhifang Wu,Xueling Lin,Fangjie Han,Zhishan Liang,Likun Huang,Mengjiao Dai,Dongxue Han,Lipeng Han,Li Niu
A simple low-cost label-free photoelectrochemical (PEC) biosensing platform based on red blood cell shaped BiVO 4 modified g-C 3 N 4 was designed for tetracycline detection under room temperature. The prepared g-C 3 N 4 /BiVO 4 heterojunction not only demonstrated a high surface area, excellent physicochemical stability and favorable PEC activity, but also can be employed as nanostructure support for aptamers to construct a visible-light-driven PEC aptasensor due to rich π-π accumulation sites. More importantly, the proposed PEC aptasensor showed a favorable linear toward tetracycline in the range from 5 × 10 -9 to 2 × 10 -7 M with a detection limit of 1.6 nM, which well covered the Food Standards Testing requirements. Practical food sample analysis further revealed the accuracy and feasibility of the g-C 3 N 4 /BiVO 4 heterostructure based PEC platform. It is expected that such a label-free and cost-effective PEC strategy should act as a promising candidate for tetracycline determination in food quality control and supervision.
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JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
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2022
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IF 5.141
Xiufeng Wang,Yunyun Zhang,Yao Jin,Siqi Wang,Zhiqing Zhang,Ting Zhou,Guodong Zhang,Fang Wang
Quantum dots (QDs) are excellent fluorescent indicators for chemical and biological analysis. In this paper, an Off-Off fluorescence sensor based on ZnS quantum dots was designed. It was found that Co 2+ can quench the fluorescence of ZnS QDs, moreover glutathione (GSH) can further quench the fluorescence. Therefore, this method can be used for indirect detection of GSH. However, if change Co 2+ into other metal ions, the system cannot be used to detect GSH indirectly. For direct detection GSH, that is adding GSH without Co 2+ , the fluorescence intensity changes little, while only amino acid or monosaccharide has no effect. When indirect detection of GSH based on this Off-Off fluorescent probe, the fluorescence quenching was linearly related to the concentration range of 1–300 μM GSH, and the detection limit could reach 0.48 μM, which can rival most Off-On and On-Off fluorescent probe. The relative standard deviation for parallel measurement of 300 μmol·L -1 GSH solution (for n = 10) was 1.43 %.
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MOLECULES
·
2022
·Vol. 27
·期 18
·5985
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IF 4.927
Yuqin Zhang,Shiquan Bian,Jing Hu,Gang Liu,Shouhua Peng,Hongjiang Chen,Zhenying Jiang,Tongyong Wang,Quan Ye,Haibo Zhu
Natural deep eutectic solvents (NADESs) coupled with microwave-assisted extraction (MAE) were applied to extract total flavonoid compounds from spent sweet potato (Ipomoea batatasL.) leaves. In this study, ten different NADESs were successfully synthesized for the MAE. Based on single-factor experiments, the response surface methodology (RSM) was applied, and the microwave power, extraction temperature, extraction time, and solid–liquid ratio were further evaluated in order to optimize the yields of total flavonoid compounds. Besides, the extracts were recovered by macroporous resin for the biological activity detection of flavonoid compounds. As a result, NADES-2, synthesized by choline chloride and malic acid (molar ratio 1:2), exhibited the highest extraction yield. After that, the NADES-2-based MAE process was optimized and the optimal conditions were as follows: microwave power of 470 W, extraction temperature of 54 °C, extraction time of 21 min, and solid–liquid ratio of 70 mg/mL. The extraction yield (40.21 ± 0.23 mg rutin equivalents/g sweet potato leaves) of the model validation experiment was demonstrated to be in accordance with the predicted value (40.49 mg rutin equivalents/g sweet potato leaves). In addition, flavonoid compounds were efficiently recovered from NADES-extracts with a high recovery yield (>85%) using AB-8 macroporous resin. The bioactivity experiments in vitro confirmed that total flavonoid compounds had good DPPH and O2−· radical-scavenging activity, as well as inhibitory effects onE. coli,S. aureus,E. carotovora, andB. subtilis. In conclusion, this study provides a green and efficient method to extract flavonoid compounds from spent sweet potato leaves, providing technical support for the development and utilization of sweet potato leaves’ waste.Keywords:natural deep eutectic solvents;response surface;spent sweet potato leaves;antioxidant activity
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2022
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IF 5.895
Xinnan Ma,Wenrui Huang,Yongqing Song,Juan Han,Jiacong Wu,Lei Wang,Yun Wang
The development of a suitable immobilization strategy to improve the performance of immobilized glucose isomerase for the isomerization of glucose to fructose is crucial to promoting the industrial production of high-fructose syrup. In this work, a novel recyclable upper critical solution temperature (UCST)-type mVBA-b-P(AAm-co-AN)@glucose isomerase biocatalyst (PVAA@GI) was prepared, and the immobilized glucose isomerase could capture the glucose substrate through the affinity of 4-vinylbenzeneboronic acid (4-VBA) and the glucose substrate, which led to the enhanced substrate affinity and catalytic efficiency of the PVAA@GI. The biocatalyst exhibited excellent stability in pH, thermal, storage, and recycling compared to the free enzyme. The mVBA-b-P(AAm-co-AN)@glucose isomerase biocatalyst displayed reversibly soluble–insoluble characteristics with temperature change, which was in the soluble state during the enzyme reaction process but could be recovered in an insoluble form by lowering the temperature after the reaction. The highest fructose production rate reached 62.79%, which would have potential application in the industrial production of high-fructose syrup.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2022
·Vol. 103
·期 6
·3006-3016
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IF 4.125
Xiaoxiang Han,Jianghui Huang,Jinwang Cai,Chen Yu,Shengchou Jiang,Qing Ye,Shuifen Yang,Weichun Pan,Qiaozhi Zhang,Yanbo Wang
Background As potent antioxidants, anthocyanins can protect the body from free radicals. However, the traditional solvent extraction method has the disadvantages of requiring a high extraction temperature and long extraction time, so it is necessary to develop an efficient extraction method for anthocyanins. Results In this study, the technique of natural deep eutectic solvents (DESs) was applied to extract anthocyanins from purple perilla leaves with the aid of microwave-ultrasonic assisted extraction (MUAE). The response surface methodology (RSM), based on the Box–Behnken design (BBD), predicted the maximum extraction yield of anthocyanins to be 619.62 mg (100 g) −1 under the following conditions: x 1 (ultrasonic extraction power) = 357.25 W, x 2 (time) = 25.62 min, and x 3 (temperature) = 57.80 °C. The biological activity of the extract obtained was evaluated by examining its radical-scavenging effect on 1,1-diphenyl-2-picrylhydrazyl, hydroxyl radical, and superoxide anion radicals. Its bacteriostatic impact was investigated on four typical bacteria: Shewanella putrefaciens ( S. putrefaciens ), Pseudomonas fluorescens ( P. fluorescens ), Escherichia coli ( E. coli ), and Staphylococcus aureus ( S. aureus ). Conclusion The integrated extraction method of DESs with MUAE was efficient, energy-saving, green, and sustainable. © 2022 Society of Chemical Industry.
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ELECTROCHIMICA ACTA
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2022
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IF 7.336
Weijie Fu,Kai Zhang,Xiaohua Zhang,Guorong Fan,Zongde Wang,Shangxing Chen,Yangping Wen,Peng Wang
Transition metal sulfide functionalized hierarchically porous carbons as colorimetric-electrochemical dual-mode nanozyme sensing platform for the visual qualitative colorimetric and quantitative electrochemical detection of dopamine (DA) are synthesized by hydrothermal method using Chinese fevervine herb rich in sulfur as the carbon source and sulfur source, and different transition metal chlorides as the metal sources. The introduction of FeCl 3 is more favorable to the generation of micropores, while the introduction of other transition metal chlorides is beneficial to the generation of mesopores and macropores. FeS 2 functionalized hierarchically porous carbon (FSPC) with large specific surface area shows better conductivity, more excellent electrocatalytic ability, and higher peroxidase-like and oxidase-like activities. The inhibitory colorimetric assay for DA designed by the oxidase-like properties of FSPC without the intervention of H 2 O 2 , making the reaction system more stable. FSPC displays excellent electrochemical responses for DA with about 50-fold peak current compared with bare electrode, and possesses a low detection limit of 0.015 μM (S/ N = 3) in a wide linear range of 0.05–1000 μM. This work will provide a new idea for the nanozyme sensing of DA with colorimetric-electrochemical dual-mode and a new possibility for application in clinical medicine.
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FUEL
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2022
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IF 8.035
Jiayu Hao,Yanqiu Wang,Kuang Sheng,Yahui Yang,Zhongliang Tian,Yang Liu,Wenzhang Li,Jie Li
Oxygen reduction reaction (ORR) is the core process of metal-air battery. Glucose and fructose are the primary monomers in biomass. In this work, the molecular configuration transformation between pyranose (glucose) and furanose (fructose) is selected as an entry point to construct thinner micropore-riched carbon skeleton with high amount of active sites. The molecular configuration transformation from furanose to pyranose increases the activation barrier associated with the molecular conversion, self-assembly, and aromatization reaction. The higher activation barrier slows the dynamic pyrolysis process, which favors the pore-forming and graphitization of precursors. The Glu-SSC-950–1 (derived from pyranose) exhibits a larger electrochemical active surface area (ECSA) and faster electron transfer, concretely, double-layer capacitance increases from 7.4 to 14.9 mF cm −2 . And the Glu-SSC-950–1 exhibits a higher half-wave potential (0.847 V) and kinetic current density at half-wave potential (3.91 mA cm −2 ) than Fru-SSC-950–1 (derived from furanose, 0.801 V, 2.92 mA cm −2 ). In practical application, the Al-air battery with Glu-SSC-950–1 exhibits a superior peak power density (467.8 mW cm −2 ) than Fru-SSC-950–1 (400.7 mW cm −2 ). In general, we present an approach for the preparation of highly active ORR biomass-derived carbon.
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JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
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2022
·Vol. 26
·期 14
·3995-4006
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IF 5.295
Cong Zhang,Yuzhen Lu,Yingying Song,Liang Chen,Junjie Hu,Yan Meng,Xin Chen,Shan Li,Guohua Zheng,Zhenpeng Qiu
Mounting evidence indicates that hepatic de novo lipogenesis is a common abnormality in non-alcoholic fatty liver disease (NAFLD) patients. We investigated whether a selective COX-2 inhibitor, celecoxib, alleviates hepatic steatosis by targeting an Akt-driven lipogenic pathway. We estimated the efficacy of celecoxib in a novel Akt-driven NAFLD mouse model established via hydrodynamic transfection of activated forms of AKT and in fructose-fed NAFLD mice that exhibited increased insulin-independent hepatic lipogenesis. AKT-transfected and insulin-stimulated human hepatoma cells were used for the in vitro experiments. Haematoxylin and eosin staining, immunohistochemistry and immunoblotting were performed for mechanistic studies. The results revealed that celecoxib ameliorated hepatic steatosis in the AKT-triggered NAFLD mice. Mechanistically, celecoxib effectively suppressed AKT/mTORC1 signalling and its downstream lipogenic cascade in the Akt-driven NAFLD mice and in vitro. Furthermore, celecoxib had limited efficacy in alleviating hepatic lipid accumulation and showed no influence on lipogenic proteins associated with hepatic lipogenesis in fructose-administered mice. This study suggests that celecoxib may be favourable for the treatment of NAFLD, especially in the subset with Akt-triggered hepatic lipogenesis.
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Frontiers in Pharmacology
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2022
·
IF 5.988
Zhang Cong,Song Yingying,Chen Liang,Chen Peng,Yuan Ming,Meng Yan,Wang Qi,Zheng Guohua,Qiu Zhenpeng
Urolithin A (UroA) is one of the primary intestinal metabolites of ellagitannins, showing translational potential as a nutritional intervention in humans. Mounting evidence suggests that fructose consumption contributes to the progression of chronic kidney disease (CKD) that manifests in hyperuricemic nephropathy, renal inflammation, and tubulointerstitial injury. Here, we investigated the efficacy of UroA in alleviating fructose-induced hyperuricemic nephropathy in mice. Uric acid-exposed human kidney-2 (HK-2) cells were utilized for in vitro mechanism validation. Histopathological staining, immunoblotting, and transmission electron microscope were performed for the mechanistic investigations. Our results revealed that UroA ameliorated fructose-induced hyperuricemic nephropathy in mice. The histopathologic assessment showed that UroA attenuated tubular hypertrophy and dilation, glomerular basement membrane thickening, and collagen deposition in the kidney of fructose-fed mice. Mechanistically, UroA treatment impaired STING-NLRP3 activation, resulting in reduced production of proinflammatory cytokines IL-1β, IL-6, and TNF-α. Notably, UroA exhibited a scavenging effect against reactive oxygen species (ROS) and restored fructose-impaired PINK1/Parkin-mediated mitophagy in nephropathic mice. Furthermore, the inhibitory effect of UroA in STING-NLRP3 activation was impaired after Parkin gene silencing in HK-2 cells. Together, this study suggests that UroA alleviates fructose-induced hyperuricemic nephropathy by promoting Parkin-dependent mitophagy, thereby suppressing STING-NLRP3 axis-mediated inflammatory response. Thus, dietary supplementation with UroA or ellagitannins-rich foods may serve as a promising intervention to prevent CKD progression.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2022
·Vol. 103
·期 2
·666-679
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IF 4.125
Jun Tian,Fengyun Cheng,Yurou Yun,Junjie Yi,Shengbao Cai,Linyan Zhou
BACKGROUND Recently, cloudy pomegranate juice (PJ) has become popular due to its rich phenolic and health-promoting effects. The aim of the present work was to evaluate the application of high hydrostatic pressure processing (HPP), pasteurization (PT) and high-temperature short-time sterilization (HTST) on physicochemical properties (color, flow behavior, turbidity, sugars, organic acids, aroma and sensory evaluation) and in vitro bioaccessibility of total phenolics content (TPC), total flavonoids content (TFC) and phenolics of cloudy PJ. RESULTS Compared to HPP, thermal sterilization significantly increased the brightness ( L *), redness ( a *), total color difference (Δ E ) and turbidity, and decreased the TPC and TFC. HPP maintained the volatile profile of cloudy PJ better, while thermal sterilization significantly changed the profile by decreasing alcohols 23.8–32.7% and increasing acids by 33.6%–182.8%. The bioaccessibility of flavonoids, phenolic acids and tannins in the control cloudy PJ after in vitro oral-gastric-intestinal digestion were 1.5%, 4.9%, and 9.0%, respectively, which were not significantly changed by different treatments. CONCLUSION These results contributed to promoting the color quality and health benefits of cloudy PJ rich in phenolics by optimizing the processing conditions in the food industry. © 2022 Society of Chemical Industry.
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APPLIED SURFACE SCIENCE
·
2022
·
IF 7.392
Jing Feng,Gang Lang,Tingting Li,Jing Zhang,Jun Zhao,Wei Li,Weiqing Yang,Zhenju Jiang
Coordination-driven self-assembly has emerged as a smart strategy to prepare advanced materials for various applications. Here we coat Ni(II)-tannic acid complexes on the lab-made C-Cr 2 O 3 nanoparticles by this strategy, and demonstrate that this novel coating dramatically accelerate the charge transfer and thus enhance the amperometric responses for electrochemical detection of glucose and hydrazine. The charge-transfer rate ( k s ) of the obtained C-Cr 2 O 3 @Ni-MPN is much higher than that of C-Cr 2 O 3 substrate, evaluated as 1.00 and 0.82 s −1 respectively. The physicochemical and electrochemical characterizations demonstrate that the enhanced k s of C-Cr 2 O 3 @Ni-MPN is attributed to the electronegativity and the network structure of its surface. Correspondingly, C-Cr 2 O 3 @Ni-MPN exhibits superior sensitivity (5043.3 μA mM −1 cm −2 ) and ultra-low detection limit (0.02 μM) for the glucose detection, as well as high sensitivity (918.5 μA mM −1 cm −2 ) and ultra-low detection limit (0.04 μM) for the hydrazine detection. Furthermore, C-Cr 2 O 3 @Ni-MPN exhibits the excellent selectivity, stability and reproducibility, and the powerful reliability for analyzing the plasma samples. This study constructs an appropriate hierarchical structure towards catalyzing the electrochemical oxidation of targets, which achieves the amplification of the respond current and thus an ultra-sensitive electrochemical detection for the targets.
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Analytical Methods
·
2022
·Vol. 14
·期 37
·3632-3637
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IF 3.532
Zhi-Ang Liu,Ya-Nan Zuo,Yinghui Xia,Jing Sun,Shuyun Zhu
An innovative strategy with target-triggered cascade fluorescence recovery of a dual-nanoquencher system was developed to detect ascorbic acid (AA). Herein, manganese dioxide (MnO2) nanosheets and gold nanoparticles (AuNPs) were used as nanoquenchers simultaneously. Owing to their synergistic effects, the fluorescence of 2,3-diaminophenazine (DAP) was decreased efficiently, thus minimizing the background fluorescence. The introduction of AA triggered the decomposition of MnO2 into Mn2+, which induced the aggregation of AuNPs. Both the decomposed MnO2 and aggregated AuNPs possess weak quenching abilities towards DAP. Such a cascade amplification strategy enhanced the detection sensitivity for AA with a LOD as low as 6.7 nM, which was two orders of magnitude lower than that of MnO2-based fluorescence assay. Furthermore, this amplification strategy was successfully applied to detect AA in food samples.
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危险品化学品经营许可证（带存储）