EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
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2020
·
IF 3.616
Dongqin Lei,Quan Wang,Yue Kong,Yuanwei Chen,Xianglin Luo
It is important to reduce side effects and to explore novel usage for hydrophobic broad-spectrum antibacterial agent triclosan (TCS). In this study, a new amphiphilic copolymer with tertiary amine groups, monomethyl ether poly(ethylene glycol)-b-poly{α-[4-(diethylamino)methyl-1,2,3-triazol]-caprolactone-co-caprolactone} (mPEG-PDCL) was designed and synthesized, and its micelles were applied as carries of TCS to enhance antimicrobial and bacteriostatic action. mPEG-PDCL and its contrastive copolymer mPEG-PCL could form uniform spherical micelles with sizes 50-110 nm . The zeta potential of mPEG-PDCL micelles was positive and changed from 7.00 ± 0.67 mV at pH 7.5 to 24.67 ± 1.23 mV at pH 5.5. Both TCS-loaded micelles displayed quite high drug loading content (approx. 15%) and drug loading efficiency (more than 85%). In comparison with pH 7.4, TCS released faster in acidic environment which was induced by bacteria metabolism. MIC values of both TCS-loaded micelles against S. aureus and E. coli were as low as free TCS. TCS-loaded micelles showed much better antibacterial activity than free TCS, especially, mPEG-PDCL/TCS micelles displayed long bacteriostatic efficacy in 60 h against S. aureus and in 54 h against E. coli . mPEG-PDCL micelles preferred targeting to both S. aureus and E. coli due to positive zeta potential. In in vivo experiment, the purulence of the infected wound almost disappeared for SD rats treated with mPEG-PDCL/TCS micelles. Therefore, mPEG-PDCL micelles may be used as good carriers for antimicrobial agents, and the TCS-loaded micelles possess long antimicrobial/bacteriostatic efficacy.
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Journal of Materials Chemistry C
·
2020
·Vol. 8
·期 9
·3029-3039
·
IF 7.059
Peitao Xie,Yifan Li,Qing Hou,Kunyan Sui,Chunzhao Liu,Xueyan Fu,Jiaoxia Zhang,Vignesh Murugadoss,Jincheng Fan,Yanpeng Wang,Runhua Fan,Zhanhu Guo
One-dimensional wires are the most common building blocks in metamaterials. In this study, zero-dimensional nanoparticles connected by tunneling networks were used to construct metamaterials, thus providing a more flexible alternative for designing the geometrical configuration of metamaterials, particularly in nanodevices. The composites with nickel nanoparticles@MnO were prepared by a bio-gel derived strategy. Nickel nanoparticles were not connected geometrically, but the conductive network had been already formed, which was a tunneling-dominated percolative phenomenon demonstrated by the first-principles calculation. Negative permittivity was achieved in the composites, as the low-frequency plasmonic state could be generated in the tunneling nickel-networks. At the same time, negative susceptibility was observed due to the diamagnetism of the tunneling current loops. Electromagnetic simulations indicate that the composites have the potential for electromagnetic shielding (only 0.25 mm in thickness). It is believed that this study not only fills up the research gap in the influence of the tunneling effect on negative electromagnetic parameters but also opens up another way of preparing metamaterials by using zero-dimensional nanoparticles instead of one-dimensional wires.
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Reaction Chemistry & Engineering
·
2020
·Vol. 5
·期 8
·1397-1404
·
IF 3.441
Xin Pan,Sihua Wu,Deshan Yao,Lian Liu,Lina Zhang,Zixuan Yao,Yan Pan,Siyuan Chang,Bingfeng Li
5-Hydroxymethyl-2-furancarboxylic acid (HMFCA) is a significant added-value intermediate derivatized from the bio-based platform chemical 5-hydroxymethylfurfuraldehyde (HMF). In the HMFCA biosynthesis process, the catalytic performance of the biocatalyst always decreases due to the presence of HMF toxicity, which is not conducive to achieving high product accumulation. Herein, we successfully obtained a new whole-cell biocatalyst from Pseudomonas aeruginosa PC-1, which was first reported to produce HMFCA from HMF. After optimization, 90.1% yield of HMFCA was attained within 6 h using a 100 mM substrate. Importantly, a much lower cell dosage was needed in the process. In order to achieve higher HMFCA accumulation, a fed-batch strategy was developed by using the growing cells from P. aeruginosa PC-1. Consequently, a total concentration of 721 mM HMFCA was obtained from 800 mM HMF within 58 h after 8 cycles of fed-batch, which is the highest concentration to date. The efficient HMFCA production made P. aeruginosa PC-1 a promising whole-cell biocatalyst for selective, large scale biotransformation of HMF.
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JOURNAL OF HAZARDOUS MATERIALS
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2020
·
IF 9.038
Yuwei Wang,Jingfeng Gao,Wanjun Duan,Wenzhi Zhang,Yifan Zhao,Jie Liu
The inactivation of a gram-negative sulfonamide antibiotic resistant bacteria (ARB) HLS.6 and removal of intracellular antibiotic resistance gene (ARG, sul1 ) and class I integrase gene ( intI1 ) by nanoscale zero-valent iron (nZVI) and sulfide-modified nZVI (S-nZVI) with different S/Fe molar ratios were investigated in this study. The S-nZVI with high sulfur content (S/Fe = 0.05, 0.1, 0.2) was superior to nZVI and the treatment effect was best when S/Fe was 0.1. The ARB (2 × 10 7 CFU/mL) could be completely inactivated by 1.12 g/L of S-nZVI (S/Fe = 0.1) within 15 min, and the removal rates of intracellular sul1 and intI1 reached up to 4.39 log and 4.67 log at 60 min, respectively. Quenching experiments and flow cytometry proved that reactive oxygen species and adsorption were involved in the ARB inactivation and target genes removal. Bacterial death and live staining experiments and transmission electron microscopy showed that the ARB cell structure and intracellular DNA were severely damaged after S-nZVI treatment. This study provided a potential alternative method for controlling the antibiotic resistance in aquatic environment.
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JOURNAL OF MOLECULAR LIQUIDS
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2020
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IF 5.065
Kishore Chand,Dianxue Cao,Diaa Eldin Fouad,Ahmer Hussain Shah,Muhammad Nazim Lakhan,Abdul Qadeer Dayo,Hemant J. Sagar,Kai Zhu,Abdalla Mohamed Ali Mohamed
In this study, Acacia catechu (AC) extract was used as a stabilizing and reducing agent to synthesize silver (Ag) and titanium dioxide (TiO 2 ) nanoparticles (NPs). By using AC extract, the synthesis process becomes more environmental friendly, economical, and advantageous. The morphology and size of synthesized Ag and TiO 2 NPs were determined by X-ray diffraction (XRD), Transmission electron microscopy (TEM), Dynamic light scattering (DLS), Atomic force microscopy (AFM), and Scanning electron microscopy (SEM). Elemental composition was determined by using Fourier transform infrared spectroscopy (FTIR) and Energy dispersive X-ray spectrometer (EDX), EDS mapping and X-ray photoelectron spectroscopy (XPS). The observations from TEM, SEM, AFM, and DLS revealed that synthesized products are spherical, hexagonal, polydispersed, and ranged within 6–20 nm size. XRD result demonstrated well crystallized and smaller size NPs are formed in both cases. FTIR and XPS analysis depicted the presence of flavonoids , terpenoids , and amino groups in addition to benzene ring agents; which act as stabilizing agents as well as possible aromatic primary amine groups may also be attached with the surface of Ag and TiO 2 NPs. Furthermore, the synthesized AgNPs exhibited strong antibacterial activity against S-aureus and catalytic application over Rose Bengal, Rhodamine B , and 4 nitrophenol dyes as compared to TiO 2 under sunlight (solar irradiation). Overall, Ag and TiO 2 NPs exhibit high potential in the development of antibacterial materials for both Gram-positive as well as Gram-negative bacteria and catalytic material for photocatalytic degradation of a certain type of hazardous dyes or chemicals, thereby paving the way for the treatment and bioremediations. The reason could be high energy and smaller size particles were active in the photo-catalysis and antibacterial performance of AgNPs compared to TiO 2 NPs.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2019
·
IF 6.361
Haosong Liu,Xuyang Liu,Fangbo Zhao,Yang Liu,Lijia Liu,Lihong Wang,Chengbao Geng,Pei Huang
In order to improve the anti-biofouling performance of ultrafiltration membrane, quaternized graphene oxide (QGO) was synthesized as a membrane modifier by in-situ growth of quaternary ammonium salt on the graphene oxide surface. A hydrophilic and antibacterial dual functional membrane was prepared with this kind of modifiers by immersion phase inversion method. The modified membrane obviously exhibits enhanced hydrophilicity, antibacterial and mechanical properties than the unmodified Polyvinylidene fluoride (PVDF) membranes. At the same time, it is proved that graphene oxide, as a carrier of quaternary ammonium salt, can significantly reduce the loss of antibacterial substances from the membranes.
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CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Ying Liu,Jingfeng Gao,Mingyan Zhao,Xiaoyu Fu,Yi Zhang,Haoran Zhang
As the emerging contaminants , antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) have caught considerable research attention, horizontal gene transfer is one of the most primary contributors to antibiotic resistance dissemination. The application of the oxidant peroxymonosulfate (PMS) as a disinfection alternative has been systemically explored. Also, the effects of various process parameters on ARB inactivation and plasmid-mediated horizontal transfer inhibition, initial bacterial concentration, pH value and temperature, were systemically investigated. The changes in cell substances (cell membrane integrity and intracellular reactive oxygen species) were determined by flow cytometry. The results suggested that the inactivation efficiency of Escherichia coli DH5α (donor) and Pseudomonas sp. HLS-6 (recipient) by 0.8 mM PMS treatment reached 4.45 log and 4.15 log in 30 min, respectively. The conjugative transfer didn’t arise after treatment for only 1 min when the dosage of PMS exceeded 0.4 mM. PMS could work in a wide pH and ambient temperature range. It also showed significant removal efficiency on intracellular ARGs and extracellular ARGs. To identify the underlying mechanisms of restraining conjugative transfer, the mRNA expression of conjugative genes, oxidative stress genes, save our souls (SOS) response-related gene, cell outer membrane proteins genes and global regulatory genes were evaluated. The results demonstrated that the expressions of these genes were altered by 0.8 mM PMS treatment. PMS has the advantages of low cost, easy operation and technical applicability. This study illustrated that PMS treatment showed great potential for controlling the antibiotic resistance for wastewater treatment .
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Chemical and Biological Technologies in Agriculture
·
2026
·
IF 5.8
Tian Shu,Cheng Feng,Wu Pan,Jia Xuewei,Qu Lili,Xu Chunping
Background Microbial exopolysaccharides (EPS) are renewable biopolymers of increasing importance in the agri-food sector, and producing them from low-cost substrates offers a route to valorize agro-industrial resources. Aureobasidium pullulans is an industrially important fungus that secretes the α-glucan pullulan; however, the structure of the EPS it produces under lactose fermentation—a means of upgrading a low-value, dairy-derived carbon source—has not been clearly defined, which limits its rational use as an agri-food bioproduct. This study aimed to produce, structurally characterize, and preliminarily evaluate the physiological activity of this EPS. Results A homogeneous EPS was obtained by ethanol precipitation, Sevag deproteinization, and gel-filtration chromatography. Fourier-transform infrared spectroscopy indicated an α-configured, neutral polysaccharide, and gas chromatography identified glucose as the sole monosaccharide. Size-exclusion chromatography coupled with multi-angle laser light scattering showed a high-molecular-weight polymer. One- and two-dimensional NMR analyses established that the EPS is a linear pullulan-type α-D-glucan with the repeating unit [→6)-α-D-Glcp-(1→4)-α-D-Glcp-(1→4)-α-D-Glcp-(1→] n, in which two α-(1→4)-linked glucose residues alternate with an α-(1→6) linkage. In a high-fat-diet-induced obese rat model, dietary supplementation with the glucan attenuated body-weight gain, improved serum lipid profiles (TG, TC, HDL-C/TC), and lowered serum endotoxin and tumour necrosis factor-α, together with enhanced colonic short-chain fatty acid production, consistent with fermentation of the glucan by the gut microbiota. Conclusions Lactose fermentation by A. pullulans yields a structurally defined, linear pullulan-type α-D-glucan, demonstrating a route to convert a low-value dairy-derived substrate into a value-added biopolymer. Its prebiotic-associated metabolic effects indicate potential as a functional ingredient for the agri-food chain and warrant further mechanistic and safety studies. Graphical abstract
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Environmental Technology & Innovation
·
2026
·
IF 7.3
Yuhan Zheng,Shuohan Feng,Tianxiang Liang,Ruxin Fang,Bolun Zhang,Siji Chen,Qiyun Li
Biochars with aerogel-like structures were prepared by incorporating mycelium. • Different methods of microbial modification result in different properties of biochars. • Biochars have excellent adsorption performance and regeneration potential for Cr(Ⅵ). • The adsorption capacities of biochars ares higher than that of most adsorbents. In this study, the microbial modification of the culture medium containing agar and potato extract was carried out by incorporating fungal mycelia. Then, the medium was freeze-dried to obtain a biomass carbon precursor with an aerogel structure. Finally, it was carbonized and activated to prepare biochar with a structure similar to “hamburger” (ALB-H), “pizza” (ALB-P), and “sandwich” (ALB-S). During this process, the differences in the physicochemical properties and adsorption performance of the biochar caused by different coating treatment methods were investigated. Among them, the specific surface area and total pore volume of ALB-P and ALB-S reached 2817.14 and 3149.46 m 2 /g, 1.51 and 1.64 cm 3 /g, respectively, which were 11.88–25.08% and 11.85–21.48% higher than that of the control group ALB (2517.97 m 2 /g and 1.35 cm 3 /g). In the adsorption test using hexavalent chromium as a model, the adsorption capacity of ALB, ALB-H, ALB-P, and ALB-S reached 415.10–552.75 mg/g at pH value of 2.0, which was superior to most adsorption materials. After 10 consecutive regeneration cycles, all adsorbents maintained more than 50% of their original adsorption capacity, demonstrating moderate recyclability that necessitates further structural optimization to boost cycling stability for real wastewater treatment applications. This study verified the effectiveness of incorporating fungal mycelia as a microbial modification method for further improving the performance of biochar, and established a new strategy for developing other biochars. Download: Download high-res image (159KB) Download: Download full-size image
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Journal of Chemical Information and Modeling
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2026
·
IF 6.4
Jiaxiong Kang,Lei Zhu,Haibo Li,Xiangyu Xie,Cong Liu,Zhiwei Feng,Yi Wang,Ouyang Mo,Chengsu Wang,Xinzi Lin,Ying Xue,Haibin Liu,Qin Ouyang
Emerging infectious diseases (EIDs) pose a critical threat to global biosecurity. Integrating bio/chemical information and artificial intelligence would bring new strategies for antimicrobial drug discovery. Herein, the Human Pathogenic Microorganisms Chemogenomics Knowledgebase (HPM-CKB) is presented as the largest domain-specific resource, consolidating chemical, genetic, and proteomic data on human-transmissible pathogens, together with multiple computational functional modules. The current release covers 7876 pathogenic proteins from 267 microorganisms (including 13,914 protein 3D structures) and 234,287 associated with bioactive molecules. HPM-CKB enables large-scale virtual screening, target identification, and drug repurposing, and integrates a large language model (LLM) for interactive queries. The computational prediction performance of HPM-CKB is corroborated by known inhibitors targeting SARS-CoV-2 replicase polyprotein 1ab. In wet-lab validations, four approved drugs (cefixime, ceftazidime, saquinavir, and rilapladib) identified via virtual screening show binding activity to SARS-CoV-2 nucleoprotein in affinity assays and inhibit SARS-CoV-2 replication in Vero E6 cells, demonstrating HPM-CKB's potential in drug repurposing. Meanwhile, two anti-Staphylococcus aureus lead compounds with novel scaffolds (CYC-HXL-9124 and CYC-HXL-9126) are identified via deep learning, and the potential target protein, cell division protein FtsZ, is subsequently prioritized using HPM-CKB (http://cgai.asia/g/pathogenDB) and experimentally validated by affinity assays. Collectively, these findings establish HPM-CKB as both a chemogenomic knowledgebase and a systematic drug development platform against EIDs.
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Advanced Healthcare Materials
·
2023
·
IF 10
Shunlan Chen,Dapeng Li,Ying Wen,Gege Peng,Kexin Ye,Yiwan Huang,Shijun Long,Xuefeng Li
Hydrogels usually are fabricated by using monomers or preexisting polymers in precursor solutions. Here, a polyelectrolyte complex biohydrogel (Bio-PEC hydrogel) made from a precursor dough, by kneading, annealing, and crosslinking the dough of two oppositely charged polysaccharides, cationic chitosan quaternary ammonium salt (HACC) and anionic sodium hyaluronate (HA), photoinitiator (α-ketoglutaric acid), crosslinker glycidyl methacrylate (GMA), and water of very small quantity is reported. Controlled kneading and annealing homogenized the dough with respect to transforming randomly distributed, individual polymer chains into tightly wound double-stranded structures, which, upon UV irradiation, covalently sparsely crosslinked into a highly entangled network and subsequently, upon fully swollen in water, results in Bio-PEC hydrogel, HACC/HA, exhibiting near-perfect elasticity, high tensile strength, and high swelling resistance. Via the same kneading and annealing, tetracarboxyphenylporphyrin iron (Fe-TCPP) metal nanoclusters are incorporated into HACC/HA to obtain photocatalytic, antibacterial, and biocompatible Bio-PEC hydrogel composite, Fe-TCPP@HACC/HA. Using SD rat models, the efficacy of Fe-TCPP@HACC/HA in inhibiting Escherichia coli ( E. coli ) growth in vitro and the ability to promote wound healing and scar-free skin regeneration in vivo, or its high potential as a wound dressing material for biomedical applications are demonstrated.
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Foods
·
2023
·Vol. 12
·期 20
·3814
·
IF 5.2
Jiahang Yu,Mingyuan Huang,Huixin Tian,Xinglian Xu
The series of biochemical reactions, metabolic pathways, and regulatory interactions that occur during the storage of meat are the main causes of meat loss and waste. The volatile compounds produced by these reactions, such as hydrogen sulfide, acids, and amines, can directly indicate changes in the freshness of meat during storage and sales. In this study, a one-pot hydrothermal method based on a surface control strategy was used to develop nanoparticles of silver with different reactivities, which were further immobilized in agar powder to develop a colorimetric sensor array. Due to the different chemical interactions with various volatile compounds, the colorimetric sensor array exhibited distinct color changes. The study demonstrates significant differences between 12 different volatile compounds and provides a quantitative and visual method to reveal rich detection indicators. The colorimetric sensor array is an economical and practical multi-analyte identification method. It has many potential applications such as food packaging, anti-counterfeiting, health monitoring, environmental monitoring, and optical filters.Keywords:colorimetric sensor array;Ag NPs;VOCs;nanosensors;chilled broiler meat
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危险品化学品经营许可证（带存储）