Plasmonics
·
2024
·
IF 3.3
Lv Xianfa,Shi Tongrui,Bai Xia,Guan Zheng,Wang Huanran,Jiang Rujian,Zhou Lu,Chen Hongyu
Creatinine level is a crucial indicator in the clinical assessment and diagnosis of renal diseases, and achieving simple and accurate detection of urinary creatinine levels in resource-limited point-of-care settings is of great significance in the timely prevention and diagnosis of kidney diseases. As a popular zero-dimensional material, gold nanoparticles (AuNPs) exhibit intriguing optical properties and thus have become a promising material for many sensing detection applications. Here, we proposed a simple, efficient, and sensitive quantitative detection of creatinine by studying the relative absorbance (Δ A ) of AuNPs in absence and presence of creatinine. The method relies on the aggregation of AuNPs via ligand-exchanged of citrate ions and creatinine on the surface of AuNPs to achieve colorimetric detection. With this assay, the limit of detection for creatinine was as low as 0.16 mM, and the dynamic detection range was 0.5 to 20 mM under optimized conditions. In our experiments, the specificity of the proposed method was investigated and successfully applied to detect creatinine in urine sample. It reveals that the proposed colorimetric protocol has demonstrated a high sensitivity and selectivity for creatinine, and has a potential practicability in clinical diagnostics.
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CHROMATOGRAPHIA
·
2012
·Vol. 75
·期 21
·1287-1293
·
IF 1.195
Wang Shuping,Li Xinchun,Yang Jianping,Yang Xiujuan,Hou Fenghua,Chen Zuanguang
In clinical medicine, urine creatinine concentration is an important marker in the evaluation of renal function and muscular dysfunctions. Herein, we reported a novel method for rapid determination of creatinine in urine by microchip electrophoresis with light-emitting diode induced fluorescence detection. Creatinine was derivatized by fluorescein isothiocyanate, and then quantitatively detected by the developed microchip LED induced fluorescence detection system. The excitation and emission wavelengths were 490 and 523 nm, respectively. The urine samples were analyzed after centrifuge and filtration. A baseline separation was obtained in <30 s using 10 mM borate buffer (pH 9.0, containing 45 mM sodium dodecylsulfate), with separation voltage of 1.5 kV. Good linearity was obtained ( r
2 = 0.9978) in the concentration range of 10.0–2.00 × 10 3 μM, and the limit of detection was 2.87 μM ( S/N = 3). The recovery was 96.0–107 %, and the interday precision was <4.5 % ( n = 6). To validate assay results, we compared the present method with the Jaffe’s colorimetric assay by measuring real urine samples. The method was reliable, sensitive, high-speed, low-cost and suitable for the routine analysis of creatinine in biofluids.
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 4
·1-13
·
IF 5.3
Yang Ruo-Yu,Wang Jin-Hua,Yu Zi-Wei,Chen Yun-Ting,Wu Mei-Juan,Huang Pin-Fang,Liu Meng-Meng
A point-of-care testing platform, consisting of smartphone, miniature electrochemical workstation, and screen-printed carbon electrode (SPCE) modified by gold nanoparticle (AuNPs) and multi-walled carbon nanotubes (MWCNTs), is fabricated for the ultrasensitive detection of paclitaxel (PTX) in human serum and injection solution. To enhance conductivity of the sensing system, MWCNTs concentration and AuNPs electrodeposition time were optimized. The AuNPs/MWCNTs effectively increase the working electrode area of SPCE by a factor of 1.46, contributing to improved electrochemical performance. The steps of electrode surface modification and the characterization of AuNPs/MWCNTs/SPCE were investigated by differential pulse voltammetry, impedance spectroscopy, X-ray photoelectron spectroscopy, and scanning electron microscopy. The sensor shows good linearity between current response and PTX concentration in 0.2 M phosphate buffer at pH = 7.4 (0.05–10 μM, with a limit of detection (LOD) of 1.7 nM) and human serum (0.5–30 μM and a LOD of 3.6 nM). The recoveries range from 89.91 to 103.36% and 91.42 to 103.73% in human serum and injection solution, respectively, with satisfactory relative standard deviation. Moreover, the sensor has excellent stability during 8 weeks and exhibits outstanding specificity and reproducibility towards PTX detection, providing a possible option for PTX determination in practical application such as therapeutic drug monitoring and drug quality control. Graphical
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2024
·
IF 3.8
Li Yang,Hai-Yan Yang,Xiao-Jie Ju,Wei Wang,Zhuang Liu,Da-Wei Pan,Rui Xie,Liang-Yin Chu
Uric acid is a signal molecule that regulates the metabolic activities of the human body, and its abnormally high levels will result in multiple complications. However, the current methods for detecting uric acid generally require high-precision instruments and professional operation. Herein, a redox-responsive photonic crystal (PC) gel for specific detection of uric acid by visualized iridescent colors with high sensitivity is successfully developed by cross-linking N-isopropylacrylamide via both macromolecular poly(ethylene glycol) acrylamide (PEG) and N,N′-cystamine-bis-acrylamide (BAC). By adjusting the PEG and BAC content, the size and concentration of magnetite nanoparticles, and magnetic field strength, the PC gel prepared can specifically detect uric acid levels with the aid of uricase and achieve a color change from orange-red to blue. Such a PC gel has excellent repeatability, and the lowest relative error of uric acid in commercial rabbit serum between the results measured by the gel and enzyme-labeled instrument is merely 6%. This study provides an effective strategy for the visualizing of uric acid in actual urine and blood with high sensitivity and specificity.
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ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Dechan Lu,Zufang Huang,Jingbo Chen,Yudong Lu
Surface-enhanced Raman spectroscopy (SERS) provides a rapid and nondestructive method for biological plasma analysis, offering unparalleled sensitivity and specificity. However, most current studies predominantly employ the drop-cast method, where liquid samples are dried on the SERS substrate for spectral recording. While effective, this method is both time-consuming and inconsistent. Additionally, the Raman spectral signal of uric acid in plasma often dominates, overshadowing other crucial biomarkers necessary for disease differentiation. In this study, we present the application of label-free SERS detection for distinguishing early stage lung cancer (LC) from healthy controls (HC) using plasma samples. Plasma was diluted, adjusted to a weakly acidic pH, and combined with silver nanoparticles (Ag NPs) for SERS analysis. To maintain the plasma in a liquid state and prevent evaporation, liquid paraffin was employed. Our findings indicate that a 10-fold dilution of plasma under weakly acidic conditions significantly enhanced diagnostic performance, achieving an area under the receiver operating characteristic curve (AUC) of 0.975. This method is featured by its simplicity and high efficiency, presenting a promising strategy for the minimally invasive diagnosis of early stage LC using SERS technology.
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ANALYST
·
2024
·Vol. 149
·期 10
·2905-2914
·
IF 3.6
Hongming Hou,Yifan Liu,Xianglong Li,Wenbo Liu,Xiaoli Gong
High cost, inherent destabilization, and intricate fixing of enzyme molecules are the main drawbacks of enzyme-based creatinine sensors. The design of a low-cost, stabilizable, and enzyme-free creatinine sensing probe is essential to address these limitations. In this work, an integrated three-dimensional (3D) free-standing electrode was designed to serve as a non-enzymatic creatinine sensing platform and was fabricated by rapid electrodeposition of a dense copper nanoparticle film on nickel foam (Cu NP film/NF). This low-cost, stable, easy-to-fabricate, and binder-free Cu NP film/NF electrode has abundant active sites and excellent electrochemical performance. Cyclic voltammetry measurements show a wide linear range (0.25–24 mM), low detection limit (0.17 mM), and high sensitivity (306 μA mM−1 cm−2). The developed sensor shows high recovery of creatinine concentration in real urine. Besides, it has better specificity, reproducibility, and robustness in detecting creatinine. These excellent results suggest that a non-enzymatic creatinine sensor based on an integrated 3D free-standing Cu NP film/NF electrode has good potential for non-invasive detection of urinary creatinine.
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JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Dan Li,Liangchen Long,Wei Xia,Weixin Zhao,Likui Feng,Xinhui Xia,Shufei He,Yu Liu,Shijie You,Liangliang Wei
Human body fluids related pollutants (BFPs) are primary precursors to disinfection by-products (DBPs) in swimming pool water (SPW). This study evaluated the degradation efficiency of ultraviolet/vacuum ultraviolet (UV/VUV) technology for the removal of three typical BFPs: urea, creatinine, and hippuric acid. The results showed that UV/VUV irradiation significantly enhanced the removal of these pollutants compared to UV alone. In addition, the observed rate constant (k obs ) of the UV/VUV system was 1.9–8.0 times higher than that of the UV/H 2 O 2 system, accompanied by a substantial 89.6 % reduction in the electrical energy per order (EEO). Urea degradation primarily involved the cleavage of C-N and C-H bonds within the urea molecule induced by VUV photolysis, whereas the degradation of creatinine and hippuric acid was mainly driven by a series of reactions (including decarboxylation, demethylation, hydroxylation, and ring opening) initiated by •OH. pH variations within the range of 6.8–8.2 exerted minimal impact on pollutant removal. However, NO 3 , humic acid, and cyanuric acid obviously inhibited the removal of BFPs. Employing UV/VUV system as a pretreatment step prior to chlorination disinfection led to a noteworthy reduction of 63.6 %-69.1 % of the adsorbable chlorine in actual SPW. Results of this study presented a green, chemical-free, and operationally simple method to mitigate DBPs formation in SPW.
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Surfaces and Interfaces
·
2025
·
IF 6.3
Shuang Yang,Meiyan Guo,Jiani Yang,Gang Xiong,Baoyi Ren,Fu Ding,Lixin You,Yaguang Sun
In this study, by hydrolysis of the phosphonate esters in these hypercrosslinked polymers (HCPs) made from two bis(α-hydroxyphosphonate) linkers (1, 4-bis[(diethoxyphosphoryl)hydroxymethyl]benzene, 1 ;4,4′-bis[(diethoxyphosphoryl)hydroxymethyl]biphenyl, 2 ) and polyaromatic compounds (9, 9′-spirobi[fluorene], SF; 1, 1′, 2, 2′-tetraphenylethene, TPE; tetraphenylmethane, TPM), respectively, two porous ( SF-1-H and SF-2-H ) and four non-porous ( TPE-1-H, TPE-2-H, TPM-1-H , and TPM-2-H ) hydrophilic HCPs were prepared and characterized in detail. In pure water systems, high adsorption capacities of creatinine and p -cresol on HCPs are exhibited, with saturated adsorption capacities ranging from 96 to 208 mg/g for creatinine and from 82 to 100 mg/g for p -cresol, respectively, with saturation adsorption being attained within 15 minutes. While in the simulated blood system, the porous polymers SF-1-H and SF-2-H show also significant adsorption capacities of creatinine (21 mg/g on SF-2-H ) and p-cresol (21 mg/g on SF-1-H and 24 mg/g on SF-2-H ). The kinetics, thermodynamics and adsorption mechanism of the adsorption process are investigated. This study not only investigated the different adsorption behaviour of creatinine and p -cresol on non-porous and porous materials under different solution conditions, but also provided a method for the preparation of adsorbents with fast and high adsorption capacity, offering new insights for future efficient perfusors.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Hui Dong,Zuying Wang,Aixin Chang,Miaomiao Zhao,Siyu Wang,Yifan Li,Chenxi Su,Jiaxin Zhou,Zhiqin Zhang,Yanli Zhou,Zhaohui Li,Maotian Xu
Homovanillic acid (HVA), the principal metabolite of dopamine, serves as a critical biomarker for central nervous system function. Its concentration fluctuations hold significant diagnostic and therapeutic monitoring value for neurological disorders including Parkinson's disease and schizophrenia. This study developed a highly selective molecularly imprinted electrochemical sensor fabricated through electropolymerization of pyrrole and 3-aminophenylboronic acid (APBA) on carbon fiber electrodes to construct HVA-templated molecularly imprinted polymer (MIP) membranes. Following template removal by electrochemical cycling in sodium tetraphenylborate buffer (pH 9.18), HVA-specific recognition cavities were formed. Significantly, methylene blue (MB) was incorporated as an internal reference signal, enabling quantitative HVA detection through ratiometric measurement of MB and HVA oxidation currents, thereby effectively eliminating environmental interference and electrode variability. Experimental results demonstrated excellent linear response in the 0.5–290 μM range with a low detection limit of 0.173 μM. Compared to conventional methods, the sensor exhibits distinct advantages such as facile fabrication, cost-effectiveness, and superior selectivity. The developed system provides a reliable approach for clinical HVA detection with significant potential for neurological disease diagnosis and treatment monitoring.
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ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Jiancong Ni,Xiaochun Xu,Bifang Yang,Liyang Liu,Weiqiang Yang,Xiaoping Chen,Guiping Chen,Qingxiang Wang,Zhenyu Lin
Hyaluronidase (HAase) detection plays a critical role in cancer diagnosis and treatment. Most HAase biosensors require the incorporation of exogenous indicators into the sensing reaction for signal transduction, which might involve complex synthesis and modification procedures or potentially interfere with enzymatic activity. Herein, based on the fact that the electrochemiluminescence (ECL) can be regulated via a bipolar electrode (BPE) through the alteration of conductivity in the enzymatic reaction solution, we develop an indicator-free HAase biosensor based on the bipolar-based ECL (BP-ECL) platform. HAase degrades negatively charged macromolecular hyaluronic acid (HA) into small anionic fragments, thereby increasing the conductivity of the sensing solution. As a result, the variation of conductivity adjusts the voltage drop across the BPE and promotes electron transfer reactions at the anodic pole, thereby enhancing the ECL emission in the reporting cell. The HAase activity can be quantified by monitoring the ECL response modulation in the BP-ECL sensing platform. Given the optimal experimental conditions, the proposed BP-ECL biosensor showed a good linear relationship with the concentration of HAase in the range of 1∼60 U/mL and a detection limit of 0.13 U/mL. The developed HAase biosensor eliminates the need for exogenous indicators in bioreaction solutions and has been successfully applied for target detection in real samples with satisfactory performance.
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Science Advances
·
2025
·Vol. 11
·期 34
·
IF 12.5
Yuwei Xu,Zhen Xiong,Peikang Zhang,Runyuan Wu,Cunzhen Li,Hui Guo,Ying Du,Xiaoxiao Zhu,Dongdong Fan,Hongzhe Fan,Yong Tian,Yun Chen,Zusen Fan
Innate lymphoid cells (ILCs) play critical roles in innate immunity, epithelial barrier protection, and tissue homeostasis. However, the maintenance machinery of intestinal tissue residency of ILCs remains elusive. Here, we show that gut microbiota is necessary for the maintenance of intestinal tissue residency of ILCs. Microbiota metabolite taurodeoxycholic acid (TDCA) binds to P2Y10 receptor on ILCs to initiate downstream Ca 2+ and RhoA signaling pathways. TDCA-P2Y10 engagement induces Zfp414 transcription to prime expression of CD69 and integrin αE on ILCs, leading to intestinal residency of ILCs. Moreover, decreased levels of TDCA or P2Y10 deficiency abrogates the intestinal residency of ILCs, resulting in severer intestinal inflammation. Of note, TDCA administration can enhance intestinal tissue residency of ILCs and promote protection against intestinal inflammation. Thus, TDCA might be used as a potential drug to treat patients with inflammatory bowel disease.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Shurui Chen,Haonan Wang,Panting Ren,Ruiyun You,Qingqiang Lin,Yudong Lu
In this study, a surface-enhanced Raman scattering (SERS) substrate based on oxidized sodium alginate (OSA)-modified silver nanoparticles (Ag NPs) was developed for the highly sensitive detection of urine biomarkers. Compared to conventional citrate-reduced Ag NPs, the OSA-Ag NPs eliminate the need for additional reducing agents, offering cost-effectiveness and alignment with green chemistry principles. OSA acts as both a stabilizer and a binding site for Ag NPs, enabling uniform nanoparticle distribution and enhanced substrate robustness. This modification significantly improves the substrate's high-salinity stability, sensitivity, and reproducibility. Furthermore, the OSA-Ag NPs substrate exhibited remarkable storage stability, maintaining 92.49 % SERS intensity after 50 days under ambient conditions, which outperforms traditional Ag colloid substrates. The OSA-Ag NPs exhibited detection limits as low as 1.9 × 10 −4 M for urea, 2.4 × 10 −7 M for creatinine, and 2.7 × 10 −7 M for bilirubin, demonstrating high sensitivity and a wide linear range. In artificial urine, the OSA-Ag NPs with high accuracy and repeatability, confirming their practical utility. These results highlight the OSA-Ag NPs substrate as a promising tool for urine biomarker detection, particularly in high-salinity environments. Its ability to maintain performance in complex biological matrices suggests potential for rapid and precise diagnostics, advancing the field of biosensing.
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