Current Research in Food Science
·
2024
·
IF 6.2
Ouyang Zheng,Xiaojie Cao,Yanqing Teng,Qinxiu Sun,Shucheng Liu
The effects of dense phase carbon dioxide (DPCD) pressure and temperature on the activity of cathepsin L in white shrimp ( Litopenaeus vannamei ) were studied. Meanwhile the effects of DPCD and heat treatment on the activity of cathepsin L were compared. The results of inactivation kinetics showed that under the same treatment temperature, the inactivation effect of DPCD on cathepsin L increased gradually with the increase of pressure and time. The effect of DPCD pressure on the activity of cathepsin L accorded with the first-order kinetic model. Under the same treatment pressure, with the increase of temperature, the inactivation effect of cathepsin L was significant at the initial stage of DPCD treatment (rapid inactivation period), and decreased with the extension of time (stable inactivation period). The effect of DPCD treatment temperature on cathepsin L activity accorded with the first-order kinetic model at 35 °C, and two-stage kinetic model at 40–60 °C. The difference of relative enzyme activity between the two treatments showed that the dependence of DPCD on temperature was greater than that of heat. E a, F and E a, S of DPCD were higher than that of heat, which indicated that cathepsin L was more easily inactivated under DPCD treatment.
研究领域:
食品科学
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生物化学
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DOI
PubMed
高影响因子
Food & Function
·
2024
·
IF 5.1
Pengfei Ren,Biqian Wei,Wanting Qin,Qingjuan Tang,Yuming Wang,Changhu Xue
This study utilized in vitro colonic fermentation to examine the impact of astaxanthin on the microbial catabolism of tryptophan. Astaxanthin significantly altered the gut microbiota and raised the tryptophan catabolism metabolite levels in an in vitro human colonic fermentation system. To eliminate the influence of substrate availability, we conducted in vitro colonic fermentation of the gut microbiota of astaxanthin-domesticated mice. We observed that the capacity of astaxanthin-domesticated gut microbiota to catalyze the conversion of tryptophan to indole and derivatives was considerably augmented. Astaxanthin significantly increased the relative abundance of Akkermansia, Ruminococcus, Bacteroides and Lactobacillus and elevated the levels of indole-3-lactic acid and indole-3-propionic acid. These results demonstrated that astaxanthin regulates tryptophan metabolism by modifying gut microbiota and increasing the levels of indole metabolites, such as indole-3-lactic acid and indole-3-propionic acid. This study provides insights into the physiological activity of astaxanthin and sheds light on the potential for enhancing tryptophan metabolism through dietary manipulation of the gut microbiota.
研究领域:
营养学
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微生物学
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代谢组学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
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IF 8.5
Xinru Wen,Yixiang Zhen,Ruizhi Yang,Huimin Gao,Nasra Seif Juma,Liang Bai,Yan Chen,Yadong Zhao
In this study, an ultra-light porous aerogel was synthesized utilizing Blueberry anthocyanin (BA) and cellulose nanofibrils (CNF), and the freshness of shrimp was assessed through the colorimetric response of the aerogel. Through modification by Vinyltrimethoxysilane (VTMO), the aerogel's surface properties were altered from hydrophilic to hydrophobic, thereby enhancing its practicality in wet environments. By adjusting the concentrations and ratios of BA and CNF, key physical characteristics such as density, porosity, and specific surface area of the aerogel can be precisely controlled. These properties influence the aerogel's capacity to adsorb gases, thereby affecting its color change reversibility, sensitivity, and stability. Among all aerogels with varying proportions of BA/CNF (1:2, 1:3 and 1:4), the one prepared with a ratio of 1:3 (BA/CNF 1:3 aerogel) exhibited a high porosity and specific surface area, demonstrating exceptional sensitivity to alkaline gases and remarkable stability under light conditions. The detection limits of total volatile basic nitrogen (TVB-N) in BA/CNF 1:3 aerogel for detecting shrimp meat spoilage were 20.02 mg/100 g at 4 °C and 29.87 mg/100 g at 25 °C respectively. This kind of composite aerogel could be used for sensitive and visual monitoring of shrimp freshness, thereby reducing food waste and offering a novel approach to intelligent development within the food packaging industry.
研究领域:
食品科学
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材料科学
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生物化学
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DOI
PubMed
高影响因子
AQUACULTURE
·
2025
·
IF 3.9
Shuai Zhang,Siying Li,Huili Tian,Ming'ao Wang,Mingsheng Lyu,Shujun Wang
Decapod Iridescent Virus 1 (DIV1) leads to high mortality rates in shrimp farming, significantly impacting the aquaculture industry economically. Currently, there are no effective treatments for controlling DIV1 infections, and management primarily relies on preventative measures. Rapid and precise diagnosis of DIV1 is crucial. In this study, we identified two DNA enzymes, MCP-1 and MCP-8, that can specifically recognize DIV1 via in vitro selection. The detection limit of MCP-1 was 6.89 copies/μL of DIV1. We developed a DNAzyme-based “turn off” biosensor for quick detection of DIV1, which is both highly specific and sensitive, by integrating SYBR Green I to enhance the signal. The detection limit of biosensor could reach 2.01 copies/μL of DIV1. Compared to conventional detection methods, this sensor delivers results in 5 min without the need for complex procedures, greatly streamlining the detection process.
研究领域:
分子生物学
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水产养殖学
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病毒学
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DOI
高影响因子
CELL BIOCHEMISTRY AND FUNCTION
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2024
·Vol. 42
·期 8
·e70027
·
IF 2.8
Jia-Xin Yu,Miao Lin,Wen-Xuan Zhang,Feng-Xue Lao,Han-Chang Huang
Oxidative stress (OS) is one of the factors that result in cell damage and the development of neurological diseases such as Alzheimer's disease (AD). Astaxanthin (ASTA), a natural compound known for its potent antioxidant properties, shows the biological activities in anti-apoptosis and antitumor. However, its specific mechanism on mitochondrial function remains unclear. This study investigated the effects of ASTA on regulation in mitochondrial function and cell apoptosis under OS induced by hydrogen peroxide (H 2 O 2 ). The results demonstrated that ASTA (0.1, 1, 10 μmol/L) protected cells form H 2 O 2 -induced cell damage and apoptosis through mitochondrial pathway. ASTA significantly reduced H 2 O 2 -induced mitochondrial dysfunctions and restored the intracellular reactive oxygen species (ROS), mitochondrial membrane potential, and respiratory capacity. These findings suggest that ASTA's antioxidant properties can benefit neurons by maintaining mitochondrial function and alleviating oxidative damage and cell apoptosis induced by H 2 O 2 . Summary Astaxanthin attenuates the cytotoxicity and oxidative damage induced by oxidative stress. Astaxanthin depresses oxidative stress-induced depolarization of mitochondrial membrane potential and restores the mitochondrial respiratory capacity. Astaxanthin inhibits the mitochondrial pathway of apoptosis induced by oxidative stress.
研究领域:
神经生物学
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药理学
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细胞生物学
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DOI
PubMed
高影响因子
CyTA-Journal of Food
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2018
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IF 1.371
Li Pan,Shouwen Zhang,Keren Gu,Na Zhang
Astaxanthin-loaded liposomes were prepared by employing a thin-film ultrasound method with the utilization of soy phosphatidylcholine. The preparation conditions of astaxanthin-loaded liposomes were optimized through response surface methodology. Under the optimum preparation conditions, the observed experimental results showed the vesicle size of astaxanthin-LL was 80.62 ± 4.52 nm with the polydispersity index of 0.20 ± 0.03, while the zeta potential was −31.80 ± 1.85 mV with the highest encapsulation efficiency of 97.68 ± 0.34%. The successful encapsulation of astaxanthin in liposomes was demonstrated by Fourier-transform infrared spectroscopy. Retention rate of 82.29% was obtained after 15 days of storage at 4°C in the stability study, which confirmed that the liposome membrane could retain astaxanthin effectively, especially at low storage temperature. Encapsulation could enhance significantly the antioxidant activity of astaxanthin. Additionally, the antioxidant activity of astaxanthin after encapsulation in liposomes was correlated with the incorporation efficiency of astaxanthin.
研究领域:
食品科学
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生物化学
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DOI
高影响因子
AQUACULTURE
·
2021
·
IF 4.242
Feichao Du,Chaoyang Hu,Xue Sun,Liuquan Zhang,Nianjun Xu
Some organic carbons were able to further promote astaxanthin accumulation in Haematococcus pluvialis under stress conditions. In this study, we demonstrate that adding 0.6 mM trisodium citrate (TSC) into the culture media increased astaxanthin content in the single cell by 2.4 folds and increased astaxanthin yield by 11.9% in H. pluvialis at day 7 under high light stress. In addition, the ROS level, which was highly associated with astaxanthin accumulation, was also increased by TSC. Subsequent comparative transcriptomic analysis revealed the mechanism underlying the promoting effect of trisodium citrate on astaxanthin accumulation. TSC treatment up-regulated significantly the expression of many enzyme encoding genes involved in TCA cycle, glucose metabolism, astaxanthin biosynthesis and fatty acids metabolism . In addition, trisodium citrate treatment significantly changed the expression of genes involved in ROS scavenging and cell wall biosynthesis. Our results indicated that TSC can not only be used as a carbon source but also further increase the ROS level in H. pluvialis under high light stress. Collectively, this study provides a new method that can increase astaxanthin yield, but also insights into the mechanism of trisodium citrate-induced astaxanthin biosynthesis in H. pluvialis under high light.
研究领域:
生物化学
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植物生理学
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DOI
高影响因子
JOURNAL OF FOOD PROCESSING AND PRESERVATION
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2021
·Vol. 45
·期 9
·e15754
·
IF 2.19
Jia-Liang Wang,Zhuo-Liang Yu,Fa-Wen Yin,De-Yang Li,Hui-Lin Liu,Liang Song,Da-Yong Zhou
The effects of different extraction solvents on the extraction yield and chemical composition of oils from by-products of marine shrimp Penaeus vannamei ( Pv ) and freshwater shrimp Procambarus clarkia ( Pc ) were investigated. Our results indicated that the ethanol and n-hexane mixture (4:1, v/v) was suitable solvent for simultaneous extraction of phospholipid and nonpolar lipids. By contrast, the ethanol and n-hexane mixture (4:1, v/v) and single ethanol were suitable solvents for the extraction of polyunsaturated fatty acids-rich (36.10%–42.78% of total fatty acids for Pv and 23.57%–25.58% of total fatty acids for Pc ) and phosphatidylcholine-rich (66.22–67.09 mol% of total phospholipids for Pv and 55.01–58.68 mol% of total phospholipids for Pc ) oils, but acetone was suitable solvent for the extraction of astaxanthin-rich oil (436.19 μg/g oil for Pv and 799.27 μg/g oil for Pc ). The findings will provide relevant information that can be used to improve the production of nutritional oils from shrimp by-products. Practical applications This study provides a theoretical basis for creating proper extraction processing to recover nutritional oils from shrimp by-products. For example, the ethanol and n-hexane mixture (4:1, v/v) was a suitable solvent for simultaneous extraction of phospholipid and non-polar lipids. By contrast, both of the ethanol and n-hexane mixture (4:1, v/v) and single ethanol were suitable solvents for the extraction of polyunsaturated fatty acids (PUFA)-rich and phosphatidylcholine-rich oils, but acetone was a suitable solvent for the extraction of astaxanthin-rich oil. Therefore, the oils extracted from shrimp by-products are rich in phospholipid, PUFA, and astaxanthin, which may be used as new functional food ingredients.
研究领域:
食品科学
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营养学
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海洋生物学
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DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2013
·
IF 2.591
Jiangmei Li,Haiyun Zhai,Zuanguang Chen,Qing Zhou,Zhenping Liu,Zihao Su
A novel method combining molecular imprinting and SPE was developed in a capillary column for the determination of auramine O in shrimp. The capillary monolithic column was prepared by UV-initiated in situ polymerization, using auramine O as template and methacrylic acid and ethylene dimethacrylate as functional monomer and cross-linker, respectively. The properties of the prepared capillary monolithic column were investigated under the optimized conditions coupled with HPLC, and then the morphologies of the inner polymers were characterized by SEM. The calibration curve was expressed as A = 103 C + 19.8 ( r = 0.9992) with a linear range of 0.25–25.0 μg/mL, and the recoveries of auramine O at different concentrations in shrimp ranged from 90.5 to 92.4% with RSDs ranging from 2.1 to 4.4%. The capacities of the molecularly imprinted polymer and nonimprinted polymer columns were 0.722 and 0.147 μg/mg, respectively, and the LOD (S/N = 3) of auramine O in shrimp was 17.85 μg/kg. Under the selected conditions, the enrichment factors obtained were higher than 70-fold. The results indicate that the prepared molecularly imprinted capillary monolithic column was reliable and applicable to the analysis of auramine O in shrimp.
研究领域:
分析化学
·
食品安全
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DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2021
·
IF 7.963
Yu Han,Wei Shi,Yu Tang,Weishang Zhou,Hongxiang Sun,Jiongming Zhang,Maocang Yan,Lihua Hu,Guangxu Liu
Gonadal development is a prerequisite for the reproductive success of an organism, and might be affected by environmental factors such as emergent pollutants. Although marine crustaceans are threatened by ubiquitous emergent pollutants such as microplastics (MPs) and bisphenol A (BPA) under realistic scenarios, studies about the impacts of these pollutants on the gonadal development of crustacean species are rare. In this study, the effects of MPs and BPA, alone or in combination, on gonadal development were investigated in whiteleg shrimp ( Litopenaeus vannamei ). The results obtained demonstrated that whiteleg shrimp exposed to MPs and BPA had significantly smaller gonad-somatic index (GSI) and an obvious delay in the gonad developmental stage. In addition, exposure of whiteleg shrimp to pollutants tested resulted in a reduction in the oxygen consumption rate, elevation in the ammonia excretion rate, decline in the O: N ratio, and downregulation in the expression of metabolism-related genes, indicating significant disruptions of shrimp metabolism by the pollutants. Furthermore, in addition to a few exceptions, both the in vivo contents of gonadal development-related hormones (GIH and MIH) and the expression of genes encoding regulatory hormones ( GIH , MIH , and CHH ) were upregulated by the exposure of whiteleg shrimp to the pollutants investigated, suggesting a significant obstruction of endocrine regulation. Moreover, MP-BPA coexposure was shown to be more toxic to whiteleg shrimp than the corresponding single exposures and significantly greater amount of BPA accumulated in the gonads (both testis and ovaries) of shrimp with the coexistence of MPs, which may be caused by the Trojan horse effect and summation of the toxic impacts on common targets. In general, the data obtained in this study demonstrated that MPs and BPA at environmentally realistic concentrations significantly inhibited the gonadal development of whiteleg shrimp probably by interfering with metabolism and disrupting endocrine regulation.
研究领域:
环境科学
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水生毒理学
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海洋生物学
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DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2017
·
IF 4.811
Guoguang Wei,Alei Zhang,Kequan Chen,Pingkai Ouyang
This study presents an efficient pretreatment of crayfish shell using high pressure homogenization that enables N -acetyl- d -glucosamine (GlcNAc) production by chitinase. Firstly, the chitinase from Serratia proteamaculans NJ303 was screened for its ability to degrade crayfish shell and produce GlcNAc as the sole product. Secondly, high pressure homogenization, which caused the crayfish shell to adopt a fluffy netted structure that was characterized by Scanning electron microscope (SEM), Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD), was evaluated as the best pretreatment method. In addition, the optimal conditions of high pressure homogenization of crayfish shell were determined to be five cycles at a pressure of 400 bar, which achieved a yield of 3.9 g/L of GlcNAc from 25 g/L of crayfish shell in a batch enzymatic reaction over 1.5 h. The results showed high pressure homogenization might be an efficient method for direct utilization of crayfish shell for enzymatic production of GlcNAc.
研究领域:
生物化学
·
废弃物利用
·
酶工程
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2014
·
IF 3.096
Zhi-Jiang Wang,Jinhyuk Lee,Yue-Xiu Si,Wei Wang,Jun-Mo Yang,Shang-Jun Yin,Guo-Ying Qian,Yong-Doo Park
To gain insight into the structural and folding mechanisms of Antarctic krill alkaline phosphatase (ALP), the enzyme was properly purified by (NH 4 ) 2 SO 4 fractionation and by both Sephadex G-75 and DEAE anion exchange chromatography . The purified enzyme (62.6 kDa; 2.62 unit/mg) was unstable at temperatures exceeding 30 °C. Denaturants, such as sodium dodecyl sulfate (SDS), guanidine HCl, and urea, were applied to evaluate the folding mechanism, including kinetics and thermodynamics, of krill ALP. Sodium dodecyl sulfate elicited no significant effect on ALP activity even at excessively high concentrations (300 mM), whereas guanidine HCl and urea effectively inactivated the enzyme at concentrations of 2 and 3.5 M, respectively. Kinetic studies showed that the enzymatic inhibition by guanidine HCl and urea represented a first-order reaction that was a monophasic unfolding process. This process was found to be associated with conformational changes without significant transient free-energy changes. Additionally, the overall structural changes occurred proximally to the active site pocket. Our study provides new insight into ALP of the Antarctic krill, which lives in extreme environmental conditions.
研究领域:
生物化学
·
海洋生物学
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产品关联
DOI
PubMed
高影响因子
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