JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2025
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IF 9.7
Qian Zhang,Meiqi Bao,Ling Zhang,Cuicui Fu,Xiaozhe Zhang,Huiting Wang,Yaxian Chen,Chunfang Zhao,Xiaoyan Ma,Jiarui Xia
The rational design of colloidal interfaces with spatiotemporal control over electromagnetic coupling is essential for enhancing the sensitivity of surface-enhanced Raman scattering (SERS) sensitivity. In this study, we propose a tyramine-triggered Ag Au nanoassembly strategy, driven by enzymatic polymerization-mediated interfacial engineering. The horseradish peroxidase/H₂O₂ system induces oxidative crosslinking of tyramine-functionalized Ag nanoparticles (Ag NPs), facilitating precise assembly with antigen-immobilized Au substrates to generate plasmonic architectures. Finite-difference time-domain simulations show that the Au Ag bilayer configuration exhibits enhanced electromagnetic field intensity compared to monolayer Au NPs or Ag NPs. This enhancement is attributed to synergistic interfacial coupling at the Au Ag nanogaps and intra-layer plasmon resonance. The dynamic nanoassembly achieved ultrasensitive aflatoxin B₁ detection with a limit of detection of 0.11 pg/mL, high interference resistance, and a wide dynamic range (0.001–100 ng/mL). The quantitative detection of AFB₁ in real samples demonstrates excellent accuracy (95.7–109.7 %) and precision (RSD < 7.5 %). By integrating enzymatic colloidal control with interfacial electromagnetic engineering, this work establishes a new paradigm for field-deployable mycotoxin monitoring in complex food systems.
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高影响因子
ANALYST
·
2025
·Vol. 150
·期 5
·914-921
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IF 3.3
Xianchen Hu,Yali Xu,Yuexi Lin,Xinghe Chen,Junshan Lin
Methods based on enzyme labelling strategies have been widely developed for capacitance immunoassays, but most suffer from low sensitivity and are unfavorable for routine use in the early stages of diagnostics. Herein, we designed a highly efficient capacitance immunosensing method for the low-abundance neuroblastoma biomarker neuron-specific enolase (NSE) using an interdigitated micro-comb electrode. Initially, monoclonal mouse anti-human NSE capture antibodies were immobilized on the interdigitated gold electrodes using bovine serum albumin. Thereafter, a sandwich-type immunoreaction was carried out in the presence of target NSE using horseradish peroxidase (HRP)-labeled secondary antibodies. The labelled HRP subsequently triggered the formation of tyramine–enzyme conjugate repeats with the help of HRP–tyramine and H2O2. The concatenated HRP molecules catalyzed the oxidation of 4-chloro-1-naphthol to produce an insoluble precipitate on the interdigitated micro-comb electrode, resulting in a shift in capacitance. Two protocols, with and without tyramine–HRP repeats, were investigated for the detection of NSE, with improved analytical performance achieved through tyramine signal amplification. Under optimum conditions, the interdigitated capacitance immunosensors exhibited good responses to target NSE within a dynamic linear range of 1.0–10 000 pg mL−1, with a low detection limit of 0.78 pg mL−1. An intermediate reproducibility of ≤9.67% was accomplished with batch-to-batch consistency, and good anti-interference capacity against other proteins was acquired. No significant differences at the 0.05 significance level were encountered in the analysis of 12 human serum specimens between the developed capacitance immunosensor and the commercially available enzyme-linked immunosorbent assay (ELISA).
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ACS Sustainable Chemistry & Engineering
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2024
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IF 7.1
Yuqi Liu,Li Yuan,Guozheng Liang,Aijuan Gu
Developing biomass resins with high thermal resistance and mechanical performance through a green strategy is an interesting challenge. Increasing the functionality of oxazine ring is favorable to improve the thermal resistance and mechanical strength of resins; however, no tri- or more functional benzoxazine monomers synthesized using the green strategy have been reported. Herein, two novel trifunctional benzoxazine monomers (coded as DPTA-f and DPTR-f) with very low melting points (61, 49 °C) were synthesized using biomass raw materials (diphenolic acid, tyramine, tyrosol, and furfurylamine) and green solvents (ethanol and water). The cured resins of DPTA-f and DPTR-f, designed as P(DPTA-f) and P(DPTR-f), not only exhibit high storage moduli (3600 and 3080 MPa) and high tensile strength (65.0 and 55.1 MPa) but also have high glass transition temperature (Tg = 328, 302 °C) and initial degradation temperature (351 and 350 °C), especially P(DPTA-f) exhibits bigger Tg than all benzoxazine resins prepared using the green strategy (SCI database). They also have good flame retardancy. The above excellent processing nature of DPTA-f and DPTR-f together with competitive thermal resistance and mechanical strength of two cured resins benefits from the unique chemical and aggregate state structures of the biobased benzoxazines.
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Biomaterials Science
·
2025
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IF 5.7
Hehua Xiong,Yiling Ruan,Huihui Liu,Xuan Liu,Xiaolian Sun
Cerenkov radiation induced photodynamic therapy (CR-PDT) offers a promising approach to overcome the dependency on external light sources and associated tissue penetration limitations. However, the therapeutic efficacy of CR-PDT is constrained by tumor hypoxia and the intrinsically short half-life and limited diffusion distance of reactive oxygen species (ROS). Herein, we propose a tumor acidity-triggered, mitochondria-targeted CR-PDT strategy to amplify the ROS generation for enhanced therapeutic efficacy. The mitochondria-targeted photosensitizers (TTCPP) are encapsulated within amphiphilic polymers, functionalized with acidity-responsive moiety and 131I labeling group, forming 131I-TTCPP nanoparticles (131I-TTCPP NPs). Under physiological conditions, 131I-TTCPP NPs exhibit minimal phototoxicity due to aggregation-caused quenching (ACQ). Upon encountering the acidic tumor microenvironment, 131I-TTCPP NPs disintegrate, restoring the photodynamic activity of TTCPP. Compared to non-targeted photosensitizers TCPP, the released mitochondria-targeted TTCPP effectively localizes to mitochondria and undergoes self-activation by 131I, generating significantly higher levels of ROS, which results in more severe mitochondrial dysfunction and enhanced apoptosis. Our findings demonstrate that coupling mitochondria targeting with self-activated CR-PDT provides a more effective and safer option for cancer treatment.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Xiaochun Wu,Yan Xu,Dandan Zhu,Weiye Zhang,Yanjun Yang,Liang Feng,Bing Yang,Xiaobin Jia
Understanding the absorption of natural polysaccharides is crucial for advancing their development and application. However, their oral absorption mechanisms and potential applications remain unclear. In this study, Astragalus homogeneous polysaccharide (APS3a) was isolated and characterized. Based on the structural analysis, in vitro and in vivo tracking of APS3a was conducted using FITC- and Cy7 amine labeling to investigate its transport and absorption mechanisms. The results indicated that APS3a can traverse the intestinal mucus layer and is mainly absorbed in the jejunum, subsequently distributing gradually to the liver and kidneys. Further investigation of its transport mechanism revealed that APS3a undergoes energy-dependent active transport, utilizing transcellular transport pathways such as caveolin-mediated endocytosis, macropinocytosis, and sodium-glucose cotransporter 1 (SGLT1)-mediated endocytosis. Additionally, APS3a has the capacity to reversibly open tight junction proteins, facilitating transport through the paracellular transport pathway. Moreover, APS3a can function as an excipient, self-assembling with flavonoids from Astragalus Radix to form complexes, thereby enhancing the permeability and absorption of these flavonoids. These findings provide valuable insights into the oral absorption of APS3a and its potential applications, which are significant for the development and application of polysaccharides.
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