Journal of Biomedical Nanotechnology
·
2016
·Vol. 12
·期 1
·69-78
·
IF 3.929
Yu Haiyang,Tang Zhaohui,Li Mingqiang,Song Wantong,Zhang Dawei,Zhang Ying,Yang Yan,Sun Hai,Deng Mingxiao,Chen Xuesi
A series of novel polypeptide-based graft copolymer poly(L-glutamic acid)-graft-methoxy poly(ethylene glycol) (PLG-g-mPEG) was synthesized through a Steglich esterification reaction of PLG with mPEG. The structure of the copolymers was confirmed by nuclear magnetic resonance
spectra (NMR) and gel permeation chromatography (GPC). MTT assay demonstrated that the PLG-g-mPEGs had good cell compatibility. The unreacted carboxyl groups of the PLG-g-mPEGs were used to complex cisplatin to form polymer-metal complex nanoparticles (CDDP/PLG-g-mPEG)
for cancer therapy. The average hydrodynamic radius of the CDDP/PLG-g-mPEG nanoparticles was in the range of 14–25 nm, which was beneficial for solid tumor targeting delivery. A sustained release without initial burst was achieved for the CDDP/PLG-g-mPEG nanoparticles,
indicating that the CDDP-loaded nanoparticles had great potential to suppress the drug release in blood circulation before the nanoparticles had arrived at targeting tumors. The CDDP/PLG-g-mPEG nanoparticles showed a much longer blood retention profile as compared with the free CDDP.
This indicated that the CDDP-loaded nanoparticles had much more opportunity to accumulate in tumor tissue by exerting the EPR effect. In vitro tests demonstrated that the CDDP/PLG-g-mPEG nanoparticles could inhibit the proliferation of HeLa, MCF-7 and A549 cancer cells. At equal
dose (4 mg kg–1), the CDDP/PLG-g-mPEG nanoparticles showed comparable in vivo antitumor efficacy and significantly lower systemic toxicity as compared with free cis-Diaminedichloroplatinum (cisplatin, CDDP) in MCF-7 tumor bearing mice. These suggested that the
CDDP/PLG-g-mPEG nanoparticle drug delivery system had a great potential to be used for cancer therapy.
研究领域:
药学
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肿瘤学
·
生物材料
✓ 已验证
DOI
PubMed
高影响因子
BIOPHYSICAL JOURNAL
·
2021
·
IF 4.033
Erika Aloi,Jin-Hua Guo,Rita Guzzi,Ren-Wang Jiang,Lucy Kate Ladefoged,Derek Marsh,Mikael Esmann,Rosa Bartucci,Natalya U. Fedosova
Spin labels based on cinobufagin, a specific inhibitor of the Na,K-ATPase, have proved valuable tools to characterize the binding site of cardiotonic steroids (CTSs), which also constitutes the extracellular cation pathway. Because existing literature suggests variations in the physiological responses caused by binding of different CTSs, we extended the original set of spin-labeled inhibitors to the more potent bufalin derivatives. Positioning of the spin labels within the Na,K-ATPase site was defined and visualized by molecular docking. Although the original cinobufagin labels exhibited lower affinity, continuous-wave electron paramagnetic resonance spectra of spin-labeled bufalins and cinobufagins revealed a high degree of pairwise similarity, implying that these two types of CTS bind in the same way. Further analysis of the spectral lineshapes of bound spin labels was performed with emphasis on their structure (PROXYL vs. TEMPO), as well as length and rigidity of the linkers. For comparable structures, the dynamic flexibility increased in parallel with linker length, with the longest linker placing the spin label at the entrance to the binding site. Temperature-related changes in spectral lineshapes indicate that six-membered nitroxide rings undergo boat-chair transitions, showing that the binding-site cross section can accommodate the accompanying changes in methyl-group orientation. D 2 O-electron spin echo envelope modulation in pulse-electron paramagnetic resonance measurements revealed high water accessibilities and similar polarity profiles for all bound spin labels, implying that the vestibule leading to steroid-binding site and cation-binding sites is relatively wide and water-filled.
研究领域:
生物物理学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Li Tang,Chunyu Wang,Sizhu Tian,Zhimin Zhang,Yong Yu,Daqian Song,Ziwei Zhang
Mn(II)-based electron spin resonance (ESR) spectroscopy was used for detecting butyrylcholinesterase (BChE) and organophosphorus pesticides (OPs). MnO2 nanosheets were synthesized with manganese chloride and hydrogen peroxide. With the catalysis of BChE, S-butyrylthiocholine iodide (BTCh) was hydrolyzed into thiocholine which has a reducing −SH group. In the presence of thiocholine, MnO2 nanosheets were broken down and Mn(IV) in MnO2 nanosheets was reduced into Mn(II). Mn2+ is a paramagnetic ion and gives a good ESR signal. In contrast, MnO2 nanosheets have no ESR signal and need not be separated from Mn2+. Mn2+ can be determined directly by ESR spectroscopy, and no further sensing probe is needed. ESR spectroscopy based on directly detecting Mn2+ is much simpler than those using other probes besides MnO2. The ESR signal of Mn2+ is proportional to the catalytic activity of BChE. OPs which inhibit the activity of BChE can also be detected by probing the ESR signal of Mn2+. Since there is no ESR signal of MnO2 nanosheets, the background signal in the absence of BChE was close to zero. The limit of detection (LOD) of BChE was as low as 0.042 U L–1. The standard curve for determining the OP paraoxon was established by measuring the inhibition of BChE by paraoxon, and the LOD of paraoxon was found to be 0.076 ng mL–1. The spiked Chinese cabbage extract samples were analyzed, and the experimental results indicated that the recoveries were from 96.5 to 102.8%. The planted Chinese cabbage was sprayed with the paraoxon solution, and the residue amount of paraoxon in the extract was estimated by the method. The result obtained by the present method was consistent with that obtained by HPLC, which proved the practicability of this new method.
研究领域:
分析化学
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环境科学
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生物化学
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DOI
高影响因子
Nano-Micro Letters
·
2025
·Vol. 17
·期 1
·1-19
·
IF 36.3
Wang Yufei,Gao Chuanlin,Lei Wen,Yang Tao,Liang Zezhou,Sun Kangbo,Zhao Chaoyue,Chen Lu,Zhu Liangxiang,Zeng Haoxuan,Sun Xiaokang,He Bin,Hu Hanlin,Tang Zeguo,Qiu Mingxia,Li Shunpu,Han Peigang,Zhang Guangye
Sequential processing (SqP) of the active layer offers independent optimization of the donor and acceptor with more targeted solvent design, which is considered the most promising strategy for achieving efficient organic solar cells (OSCs). In the SqP method, the favorable interpenetrating network seriously depends on the fine control of the bottom layer swelling. However, the choice of solvent(s) for both the donor and acceptor have been mostly based on a trial-and-error manner. A single solvent often cannot achieve sufficient yet not excessive swelling, which has long been a difficulty in the high efficient SqP OSCs. Herein, two new isomeric molecules are introduced to fine-tune the nucleation and crystallization dynamics that allows judicious control over the swelling of the bottom layer. The strong non-covalent interaction between the isomeric molecule and active materials provides an excellent driving force for optimize the swelling-process. Among them, the molecule with high dipole moment promotes earlier nucleation of the PM6 and provides extended time for crystallization during SqP, improving bulk morphology and vertical phase segregation. As a result, champion efficiencies of 17.38% and 20.00% (certified 19.70%) are achieved based on PM6/PYF-T- o (all-polymer) and PM6/BTP-eC9 devices casted by toluene solvent.
研究领域:
材料科学
·
能源技术
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Mohamed Eid,Jingsong Zhu,Muhammad Asif Ismail,Bin Li
Chemically cross-linked hydrogel nanoparticles (HGNPs) offer enhanced properties over their physical counterparts, particularly in drug delivery and cell encapsulation. This study applied pH-thermal dual responsive bio-adhesive HGNPs for dual complexation and enhanced the controlled release and bioavailability of cisplatin (CDDP) and Vitamin E (VE) drugs. The CDDP was loaded into the HGNPs via chemical conjugation with the carboxyl groups in the HGNPs surface by soy polysaccharides (SSPS). At the same time, the host-guest interaction complexed the VE through the β-cyclodextrin (β-CD). The HGNPs showed a uniform HGNPs size distribution of 90.77 ± 14.77 nm and 81.425 ± 13.21 nm before and after complexation, respectively. The FTIR, XRD , XPS , and zeta potential confirmed the conjugation. The cumulative release percent of CDDP reached 98 % at pH 1.2, while <45 % was released at pH 7.4. Our HGNPs enhance the incorporation of CDDP by substituting its chlorides with carboxyl groups of the SSPS; the loading of CDDP and VE was 15 ± 0.33 and 11.32 ± 0.25 wt%, respectively. Moreover, the CDDP and VE also released slower from the HGNPs at 25 °C than at 37 °C and 42 °C. The (VE/CDDP)-loaded HGNPs exhibited longer circulation time in vivo than free CDDP and free VE suspension.
研究领域:
药学
·
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2024
·
IF 10
Qi Ding,Yun Zhao,Enpeng Xi,Kangning Liu,Nan Gao,Guangshan Zhu
Due to the unclear pathogenesis of Alzheimer's disease (AD) and the lack of a completely cured medication, AD patients need to take medication in order and on time every day all one's life, which is difficult for severe memory impairment patients to strictly follow on time. Traditional AD drug carriers, such as sugar coating and capsules, rely on dissolution or fragmentation to achieve drug release, which lacks the interaction between drug molecules and carriers, thus they cannot achieve sufficient long-acting and sequential drug release. Herein, Mn-MOF-74, which ligand structure is similar to two antioxidants dihydroquercetin (DHQ) and resveratrol (Res) is chosen as the carrier. Due to the differences in adsorption energy between DHQ/MOF and Res/MOF, the release speed of DHQ is much faster than Res. Therefore, Mn-MOF-74 loaded with DHQ and Res (DR@MOF) showed sequential drug release and a long-term antioxidant effect for ≈72 h, with an efficacy time six times longer than that of vitamin E. In 5×FAD transgenic mice, DR@MOF exhibited excellent capacity in maintaining oxidative balance in the brain, ameliorating spatial learning and memory deficits, and showed the potential of an AD agent for long-acting and sequential treatment.
研究领域:
药学
·
神经科学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Ovarian Research
·
2024
·Vol. 17
·期 1
·1-17
·
IF 3.8
Sui Xue,Gao Bingqing,Zhang Liu,Wang Yanmin,Ma Junnan,Wu Xingchen,Zhou Chenyu,Liu Min,Zhang Lin
Background Cisplatin (DDP) is one of the most effective anticancer drugs, commonly used to treat advanced ovarian cancer (OC). However, DDP has significant limitations of platinum-based drugs, including chemical resistance and high-dose toxic side effects. Traditional Chinese medicines (TCMs) often presented in the form of formula, in which the herb pair was the basic unit. Scutellaria barbata D.Don and Hedyotis diffusa Willd (SB-HD) are famous TCMs herb pair that have been shown to help treat multiple types of cancers. However, the synergistic effects and mechanism of combination of SB-HD and DDP to enhance DDP chemosensitivity in OC are still unknown.Results In vitro, we found that the optimal proportion of SB-HD to inhibit the proliferation of OC cells was 2:1, SB-HD and DDP were shown to synergistically reduce the viability of OC cells, inhibit the colony formation, promote cell cycle arrest and apoptosis, as well as inhibit cell migration and invasion. In vivo, combination treatment significantly inhibited the growth of subcutaneous tumors in BALB/c nude mice and reduced the toxic side effects of DDP. Mechanistically, SB-HD and DDP combination treatment significantly promoted oxidative stress response, decreased MMP, inhibited ATP production, decreased ROS levels and increased SOD activity, increased the expression of NRF2, HO-1, ATG5 and LC3, decreased the expression of p62 and FTH1 both in OC cells and tumor tissue of mice. Inhibitor 3-MA (Methyladenine, autophagy inhibitor) and Fer-1 (Ferrostatin-1, iron ion inhibitor) can effectively reverse the expression changes of the key target proteins, but not ZnPP (Zinc protoporphyrin, HO-1 inhibitor). Through bioinformatics analysis, it was found that the abnormal expression level of NRF2 and FTH1 mRNA has a high prognostic value, at the same time, the other four key proteins respectively or interacting with NRF2 and FTH1, also play important roles in the occurrence and development of OC.Conclusion Our findings uncover a synergistic effect of SB-HD and DDP against OC through modulating oxidative stress via NRF2-FTH1 autophagic degradation pathway, which may provide an important theoretical foundation for the use of SB-HD and a new strategy for enhancing DDP chemosensitivity as well as reducing toxic side effects.
研究领域:
肿瘤学
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药理学
·
传统中药学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHYSICS D-APPLIED PHYSICS
·
2025
·Vol. 58
·期 13
·135207
·
IF 3.2
Liu Jinren,Wang Xiangni,Cui Yixin,Xu Guimin,Lu Jiajia,He Zhirou,Xu Yulin,Li Rongrong,Gao Lingge,Wang Hong,Gao Junhong,Wu Xili,Shi Xingmin,Zhang Guanjun
Cisplatin resistance coupled with adverse effects presents substantial hurdles in the treatment of ovarian cancer. Both cold atmospheric plasma and plasma-activated liquid (PAL) hold the ability to induce DNA damage, which is a primary target of cisplatin. In this study, we investigate the potential of PAL in augmenting cisplatin sensitivity or diminishing the necessary drug dosage by impairing the DNA of cisplatin-resistant cancer cells. We found that plasma irradiated medium exceeding 40 s could decrease the viability, induce DNA damage, trigger cell cycle arrest, and augment apoptosis and autophagy in two types of cisplatin-resistant ovarian cancer cells (A2780/DDP and SKOV3/DDP) after a 24 h incubation period. Notably, pre-treatment with 40 s plasma-activated medium (PAM) for 2 h significantly enhanced the anti-cancer effect of cisplatin administered at lower doses on these cell types. RNA-seq, quantitative real-time PCR and Western blotting analysis revealed that PAM treatment could downregulate the expression of several pivotal proteins involved in nucleotide excision repair (NER) and homologous recombination repair (HRR). The mouse xenograft tumor model demonstrated that plasma-activated saline and cisplatin synergistically inhibited tumor growth and significantly suppressed the expression of proteins related to HRR and NER pathways. We identified plasma-derived peroxide generation in PAM as crucial in these processes. These results pave the way for considering PAM as an adjuvant treatment for cisplatin-resistant ovarian cancer and other abdominal cancers. Additionally, the findings indicate the feasibility of reducing cisplatin dosage to alleviate side effects through sensitization.
研究领域:
肿瘤学
·
分子生物学
·
医学
✓ 已验证
产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Shuting Liu,Guorui Liu,Mingxuan Wang,Linjun Qin,Qingyu Guo,Da Li,Lili Yang,Minghui Zheng
Organic free radicals are critical intermediates for the generation and inhibition of organic pollutants during industrial processes. Clarifying the free radical mechanism of pollutant inhibition is significant for their efficient control. Ammonium sulfate is intensively used in industrial materials to suppress organic pollutants. In this study, organic free radical intermediate species in metal-catalyzed reactions inhibited by ammonium sulfate were identified using continuous-wave electron paramagnetic resonance (EPR) spectroscopy, providing direct evidence for the free radical mechanisms of organic pollutants inhibition. The transverse (T2) and longitudinal (T1) relaxation time variations catalyzed by different metal catalysts in the presence of ammonium sulfate were compared using pulsed-wave EPR. Consequently, after the addition of ammonium sulfate, the observed increase in T2 suggests that ammonium sulfate leads to radical concentration reduction. A decrease in the T1 relaxation time suggests the enhanced interaction between organic radicals and metals, which is an obstacle to subsequent radical reactions. Therefore, ammonium sulfate dominantly changed the free radical intermediates species, concentrations, and their reactivity, and then inhibited the organic pollutants formations. The inhibition mechanisms of ammonium sulfate on metal-catalyzed pollutants were then proposed combining EPR analysis, X-ray characterization, and high-resolution mass spectrometry screening. As a result, (1) occupying the active sites of metal catalysis and (2) inhibiting free radical intermediates are the two main intrinsic inhibition mechanisms of ammonium sulfate. The findings provide new perspectives on the efficient inhibition of organic pollutants in industrial processes involving various metal catalysts.
研究领域:
环境科学
·
化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2024
·
IF 7.1
Li Ren,Kaixin Guo,Ruirui Cui,Tingyu Zhao,Xu Wang,Min Zhang,Xin Zhang,Chaoyong Deng
Superparaelectric (SPE) relaxor ferroelectrics are emerging as the primary candidates for electrostatic dielectrics due to their superior energy storage capabilities. However, there is a lack of systematic studies on the intrinsic mechanisms that enhance energy storage performance. Here, by controlling the annealing temperature (Tan), we comprehensively analyzed the evolution of domain structures in (Ba0.985,Na0.015)(Ni0.05,Ti0.95)O3 (BNTN) dielectric films to explore the internal mechanisms enhancing the energy storage performance. Our results demonstrate that lowering Tan reduces the film’s crystallinity, transforming internal domain structures from microdomains to large polar nanoregions (PNRs) with some nanodomains and then to small PNRs and finally to the nonpolar state, thereby nearly eliminating polarization switching hysteresis. Simultaneously, the specific Tan for the SPE state enables the presence of hexagonal BNTN, endowing the films with high polarization. Additionally, the unique nanocrystalline grains and disordered amorphous-like phase lead to a high breakdown strength of 6.4 MV cm–1 for the BNTN films annealed at 650 °C. These factors synergistically contribute to an excellent energy storage density (92.8 J cm–3) and ultrahigh efficiency (90%) in SPE films. Furthermore, the SPE films also exhibit excellent fatigue reliability (107 cycles) and temperature stability (30 to 200 °C), as well as outstanding discharge properties. This study offers new insights for achieving high energy storage performance in monolithic thin films.
研究领域:
材料科学
·
电子工程
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
·
IF 4.9
Shan Wang,Youmei Wang,Lu Xiang,Jinqiu Qi,Yongze Jiang,Jiulong Xie
Alkaline lignin and sodium lignosulfonate were chemically modified by using maleic acid for potential application on the production of ultra-flexible gelatin films. The comparison results in morphology and chemical structures of the two types of lignin before and after modification revealed that carboxyl and C C groups were successfully grafted onto the lignin, resulting in the changes in physical-chemical properties. Compared to modified sodium lignosulfonate, modified alkaline lignin (MAL) was much more appropriate in the preparation of gelatin (GEL)/lignin films by endowing the films with transparency and excellent UV light shielding ability. Elongation at break of GEL/MAL films were more than two times that of the pure gelatin film, which greatly addressed the brittleness issues of the pure gelatin films during use, indicating that the modified alkaline lignin could be used as renewable additive with replacement of glycerol. The results in this study also gave evidence that the sources and structure of lignin affect its compatibility with gelatin solution. The higher weight loss rate and lower pyrolysis degradation temperature indicated that the gelatin/lignin films were much more easily thermal degradable at the end of service life. This research provided a potential and efficient approach on the production of renewable and biological films from gelatin and lignin.
研究领域:
材料科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Polymer Materials
·
2024
·
IF 4.4
Zhendong Ma,Jianshuo Zheng,Chenyue Wang,Pengli Lei,Qiu Zhuang,Weijin Li,Aming Xie
Dielectric loss and magnetic loss are two necessary factors in electromagnetic wave absorption, but there are few works that can study magnetic factors alone because of the limitations of the material itself. This work achieved the transformation from a nonmagnetic polymer (PFc-1) to a superparamagnetic polymer (PFc-3) by introducing alkyne units to regulate the valence state of iron atoms in the ferrocene unit. It also accomplished the transition of wave-absorbing properties from nonexistent to present. The extremely low dielectric loss of the polymer itself also provides a good background for studying magnetic losses. After characterization, the maximum reflection loss of PFc-3 under the action of exchange resonance reaches −31.7 dB. Through density functional theory (DFT) calculations, we can speculate on the specific mechanism of exchange resonance in PFc-3: under the influence of specific external sources, the single-electron energy level structure of Fe changes. The frequency jumps, creating a resonance. This work opens up a perspective for the application of magnetic regulation in the field of electromagnetic absorption.
研究领域:
材料科学
·
电磁学
·
计算化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）