CHEMOSPHERE
·
2021
·
IF 7.086
Xiaoning Li,Hailong Liu,Weiben Yang,Hongjie Sheng,Fang Wang,Jean Damascene Harindintwali,H.M.S.K. Herath,Yinping Zhang
The search for nature-based tools to enhance bioremediation is essential for the sustainable restoration of contaminated ecosystems. Humic acid (HA) is an important component of organic matter in soil and water, but its effect on the microbial degradation of organic pollutants remains unclear. In this study, the biodegradation of pyrene by Mycobacterium sp. NJS-1 with and without HA was investigated. Only around 10.5% of pyrene was biodegraded in the pyrene treatment alone, whereas the addition of HA significantly enhanced biodegradation to the point where over 90% of pyrene was biodegraded. The production of 4,5-dihydropyrene-4,5-diol and phenanthrene-3,4-diol indicated the metabolic pathway via attacking of 4,5-positions of pyrene. Interestingly, 1,2-dimethoxypyrene was detected with the addition of HA, suggesting that HA induced a new ring-opening pathway involving the attack on the 1,2-positions of pyrene. The addition of HA first induced protein self-cleavage behavior with a significant increase in phenylalanine , tyrosine, and tryptophan containing large numbers of COO − groups. Furthermore, it altered the intracellular and extracellular ultrastructure of bacterial cells, promoting their growth in size and number as well as reducing the space between them. Overall, HA increased the ring-opening positions of pyrene and facilitated its interaction with bacterial cells, thus improving its biodegradability . Building upon the findings of this study to further research is conducive to the sustainable solution of environmental pollution .
研究领域:
环境科学
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微生物学
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DOI
PubMed
高影响因子
INFLAMMATION
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2021
·Vol. 44
·期 5
·1993-2005
·
IF 4.092
Wu Yuqin,Fan Lin,Wang Yun,Ding Jing,Wang Rongfu
This study is aimed at exploring the potential of isorhamnetin in protection against diabetes-exacerbated ischemia/reperfusion-induced brain injury and elucidating its action mechanism. After establishment of the model of high glucose (HG)-aggravated oxygen-glucose deprivation and reoxygenation (OGD/R), HT22 cell viability was detected by CCK-8. Lactate dehydrogenase (LDH) activity, casapase-3 activity, and oxidative stress-related markers in HT22 cells were detected by corresponding commercial kits. The apoptosis of HG-treated HT22 cells following OGD/R was observed with TUNEL staining. The level of pro-inflammatory cytokines was examined by ELISA. The expression of Akt/SIRT1/Nrf2/HO-1 signaling-related proteins was assayed by Western blot. The results showed that HG noticeably worsened the OGD/R-induced apoptosis of HT22 cells. Isorhamnetin relieved the HG-aggravated OGD/R-induced apoptosis, inflammatory response, and oxidative stress of HT22 cells. Isorhamnetin alleviated the HG-aggravated OGD/R injury in HT22 cells through Akt/SIRT1/Nrf2/HO-1 signaling pathway. Meanwhile, treatment with Akt inhibitor LY294002 reversed the protective effects of isorhamnetin against HG-aggravated OGD/R injury in HT22 cells. In a conclusion, Isorhamnetin alleviates HG-aggravated OGD/R in HT22 hippocampal neurons through Akt/SIRT1/Nrf2/HO-1 signaling pathway.
研究领域:
神经科学
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药理学
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细胞生物学
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分子生物学
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DOI
PubMed
高影响因子
CHEMICAL PAPERS
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2016
·Vol. 70
·期 5
·585-593
·
IF 1.326
Lin Zhu,Fang-Fang Li,Ji-Qin Zhu,Zhi-Gang Lei
Olefins and paraffins are important basic chemical raw materials with so similar molecular structure and volatility that their separation is a difficult and energy-consuming process. Liquid–liquid equilibrium (LLE) data were determined for ternary systems of: 1-hexene + hexane + solvent at 25°C, 30°C, and 35°C, and N-formylmorpholine (NFM), N-methyl-2-pyrrolidone (NMP), 7-butyrolactone (GBL), and 1-methylimidazole (1-MEI) as the solvents studied. Liquid–liquid equilibrium data for each system were correlated to the NRTL (Non-Random Two Liquid) equation and the interaction parameters presented. The calculated results agree well with the experimental data.
研究领域:
化学工程
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物理化学
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DOI
高影响因子
JOURNAL OF SOLID STATE CHEMISTRY
·
2025
·
IF 3.5
Yuan Li,Shengkai Sun,Hanfang Liu,Wenna Wang,Dehong Chen,Yu Yang,Feng Liu,Bin Yu,Jia Liu,Binjie Li,Lei Wang,Bin Li
Epoxidation of olefin catalyzed by titanium silicalite-1 (TS-1) can obtain important intermediate epoxides of industrial production in a green and pollution-free manner. However, due the problem of macromolecules transport path restraint, which cause the catalyst to fail in lifetime and activity. Strategically, to increase the transport channel of macromolecules and shorten the transport path, it is necessary to introduce mesoporous structures into zeolite. Here, we report a solid-state synthesis of hierarchical TS-1 zeolite by optimizing carbon black sequence. The texture property of hierarchical TS-1 can be optimized by carbon black. Most importantly, the addition sequence of carbon black can impact the porosity of hierarchical TS-1. The hierarchical zeolite is synthesized by incorporating 0.2 g carbon black into the seeds. When carbon black is firstly combined with seeds, the enhanced interaction facilitates the subsequent growth process, leading to large surface area from mesopores (121 m 2 /g) and abundant pores size (8–40 nm) and enhanced exposure of Ti active sites. The optimized hierarchical TS-1 possesses excellent catalytic performance with high conversion rate (24.4 %) and similar selectivity (93.8 %), which are 1.6 times higher than that of traditional TS-1 zeolites.
研究领域:
材料科学
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催化化学
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DOI
高影响因子
Cell Metabolism
·
2020
·
IF 21.567
Wanfeng Xu,Yuan Che,Quan Zhang,Hai Huang,Chujie Ding,Yun Wang,Guangji Wang,Lijuan Cao,Haiping Hao
Summary Caspase-4 is an intracellular sensor for cytosolic bacterial lipopolysaccharide (LPS) and underlies infection-elicited pyroptosis. It is unclear whether and how caspase-4 detects host-derived factors to trigger pyroptosis. Here we show that mitochondrial permeability transition (MPT) activates caspase-4 by promoting the assembly of a protein complex, which we term the Apaf-1 pyroptosome, for the execution of facilitated pyroptosis. MPT, when induced by bile acids, calcium overload, or an adenine nucleotide translocator 1 (ANT1) activator, triggers assembly of the pyroptosome comprised of Apaf-1 and caspase-4 with a stoichiometry ratio of 7:2. Unlike the direct cleavage of gasdermin D (GSDMD) by caspase-4 upon LPS ligation, caspase-4 activated in the Apaf-1 pyroptosome proceeds to cleave caspase-3 and thereby GSDME to induce pyroptosis. Caspase-4-initiated and GSDME-executed pyroptosis underlies cholestatic liver failure. These findings identify Apaf-1 pyroptosome as a pivotal machinery for cells sensing MPT signals and may shed light on understanding how cells execute intrinsic pyroptosis under sterile conditions.
研究领域:
免疫学
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细胞生物学
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分子生物学
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DOI
PubMed
高影响因子
Cardiovascular Diabetology
·
2024
·Vol. 23
·期 1
·1-19
·
IF 8.5
Tang Yuan-juan,Zhang Zhen,Yan Tong,Chen Ken,Xu Guo-fan,Xiong Shi-qiang,Wu Dai-qian,Chen Jie,Jose Pedro A.,Zeng Chun-yu,Fu Jin-juan
Background Diabetic cardiomyopathy (DCM) is a serious complication in patients with type 1 diabetes mellitus (T1DM), which still lacks adequate therapy. Irisin, a cleavage peptide off fibronectin type III domain-containing 5, has been shown to preserve cardiac function in cardiac ischemia–reperfusion injury. Whether or not irisin plays a cardioprotective role in DCM is not known. Methods and results T1DM was induced by multiple low-dose intraperitoneal injections of streptozotocin (STZ). Our current study showed that irisin expression/level was lower in the heart and serum of mice with STZ-induced TIDM. Irisin supplementation by intraperitoneal injection improved the impaired cardiac function in mice with DCM, which was ascribed to the inhibition of ferroptosis, because the increased ferroptosis, associated with increased cardiac malondialdehyde (MDA), decreased reduced glutathione (GSH) and protein expressions of solute carrier family 7 member 11 (SLC7A11) and glutathione peroxidase 4 (GPX4), was ameliorated by irisin. In the presence of erastin, a ferroptosis inducer, the irisin-mediated protective effects were blocked. Mechanistically, irisin treatment increased Sirtuin 1 (SIRT1) and decreased p53 K382 acetylation, which decreased p53 protein expression by increasing its degradation, consequently upregulated SLC7A11 and GPX4 expressions. Thus, irisin-mediated reduction in p53 decreases ferroptosis and protects cardiomyocytes against injury due to high glucose. Conclusion This study demonstrated that irisin could improve cardiac function by suppressing ferroptosis in T1DM via the SIRT1-p53-SLC7A11/GPX4 pathway. Irisin may be a therapeutic approach in the management of T1DM-induced cardiomyopathy.
研究领域:
内分泌学
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心脏病学
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分子生物学
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药理学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2021
·Vol. 57
·期 1
·516-524
·
IF 3.713
Wangshu Zhang,Luyang Jing,Huiyun Chen,Sheng Zhang
Summary The effect of carnauba wax-based nanoemulsion coating-1 (NC-1) combined with 1-methylcyclopropene (1-MCP) treatment on the storage of honey peach fruit ‘Yulupantao’ for 28 days under 0 ± 0.5 °C, and 90% RH conditions was studied. The results showed that NC-1 or 1-MCP treatment alone and in combination were proved to be more effective in maintaining fruit qualities compared to the control group with no treatment. However, the application with the combination of NC-1 and 1-MCP treatment had the most significant effect on reducing mass loss and decay rate, maintaining firmness, total soluble solids (TSS), ascorbic acid (AsA) and titratable acid (TA) contents, suppressing malondialdehyde (MDA) accumulation and conductivity ascend. Moreover, the activities of peroxidase (POD) were maintained, and polyphenol oxidase (PPO) and phenylalanine ammonialyase (PAL) were effectively suppressed in NC-1, 1-MCP and combined treatment than that of control. Therefore, the combination of NC-1 and 1-MCP treatment could be an efficient way to maintain the qualities of peach fruit during low-temperature storage after harvest.
研究领域:
食品科学
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植物生理学
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产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2021
·
IF 6.165
Yuwei Zuo,Yue Sun,Wenzhong Yang,Kegui Zhang,Yun Chen,Xiaoshuang Yin,Ying Liu
The performance and mechanism of typical scaling inhibitors, i.e., 1-hydroxy ethylidene-1,1-diphosphonic acid (HEDP) and 2-phosphonobutane-1,2,4-tricarboxylic acid (PBTCA), in inhibiting calcium carbonate scaling were investigated. Results of the static experiment at 30 °C and 80 °C revealed that at the optimal concentration, the scaling inhibition efficiency of PBTCA was superior to that of HEDP. Additionally, X-ray powder diffraction evaluation demonstrated that in comparison with HEDP, PBTCA inhibited the growth of the (1 0 4) crystal plane of calcite more evidently. Scanning electron microscopy analysis indicated that the addition of scaling inhibitors induced the crystal transformation of CaCO 3 from calcite to aragonite. Molecular dynamics simulation also confirmed that the interaction of PBTCA with the (1 0 4) crystal surface of calcite is more apparent than that of HEDP. This observation was a consequence of the PBTCA being more negatively charged, as determined via a quantum chemical calculation. The calculation further indicated that the spatial distance of PBTCA to the (1 0 4) face of calcite was less due to stronger chelating ability and better suitability of the structure. As a result, in the tested temperature range, PBTCA exhibited the more effective inhibitory effect against calcium carbonate scaling.
研究领域:
化学工程
·
材料科学
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DOI
高影响因子
FREE RADICAL BIOLOGY AND MEDICINE
·
2014
·
IF 5.71
Simin Yan,Xu Zhang,Haili Zheng,Danhong Hu,Yongtian Zhang,Qinghua Guan,Lifang Liu,Qilong Ding,Yunman Li
Proinflammatory cytokine TNF-α-induced adhesion of leukocytes to endothelial cells plays a critical role in the early stage of atherosclerosis . Oxidative stress and redox-sensitive transcription factors are implicated in the process. Thus, compounds that mediate intracellular redox status and regulate transcription factors are of great therapeutic interest. Clematichinenoside (AR), a triterpene saponin isolated from the root of Clematis chinensis Osbeck , was previously demonstrated to have anti-inflammatory and antioxidative properties. However, little is known about the exact mechanism underlying these actions. Thus we performed a detailed study on its effect on leukocytes–endothelial cells adhesion with TNF-α-stimulated human umbilical vein endothelial cells (HUVECs) and cell-free systems. First, we found that AR reduced TNF-α-induced VCAM-1 and ICAM-1 expression and their promoter activity, inhibited translocation of p65 and phosphorylation of IκBα , suppressed IκB kinase-β (IKK-β) activity, lowered O 2 ∙− and H 2 O 2 levels, tackled p47 phox translocation, and decreased NOX4 NADPH oxidase expression. Second, we showed that AR exhibited no direct free radical scavenging ability in cell-free systems at concentrations that were used in intact cells. Besides, AR had no direct effect on the activity of IKK-β that was extracted from TNF-α-stimulated HUVECs. We also found that p47 translocation, NOX4 expression, and reactive oxygen species (ROS) levels were up-regulated before IκB phosphorylation in TNF-α-induced HUVECs. Moreover, TNF-α-enhanced IKK-β activity was also inhibited by (polyethylene glycol) PEG–catalase, N -acetylcysteine (NAC), and vitamin E. In conclusion, these results suggest that AR reduces VCAM-1 and ICAM-1 expression through NADPH oxidase-dependent IKK/NF-κB pathways in TNF-α-induced HUVECs, which finally suppress monocyte–HUVECs adhesion. This compound is potentially beneficial for early-stage atherosclerosis.
研究领域:
药理学
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免疫学
·
心血管研究
·
分子生物学
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DOI
PubMed
高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
·
2021
·
IF 5.455
Mengyao Li,Yi Zhai,Xubin Zhang,Fumin Wang,Guojun Lv,Ahishakiye Rosine,Mengyue Li,Qing Zhang,Yongkui Liu
The preparation of hollow zeolite with a large cavity structure to improve the accessibility to the active sites of catalyst is of great significance. A thin-walled Ti-rich hollow Titanium silicalite-1 (TS-1) Nanobox was synthesized by dissolution-recrystallization using Silicalite-1 (S-1) as the parent zeolite and (NH 4 ) 2 SO 4 as the dual-function crystallization-mediating agent. The wall thickness of the zeolite is about 30 nm, the hollow percentage (the ratio of the cavity area in the middle of the zeolite to the overall area of the zeolite) can reach 0.57, and the Si/Ti ratio is 39.63. The formation mechanism of hollow TS-1 was studied. As the parent zeolite, S-1 is easier to dissolve than TS-1, making it easier for the hollow zeolite to get a complete cavity. When the molar ratio of (NH 4 ) 2 SO 4 /TEOS reaches 0.25, the dissolution rate matches the crystallization rate best, and a perfect hollow structure is obtained. The pH of the initial gel is the key factor for the hollow structure. The catalytic activity and stability were tested by 1-hexene epoxidation. The conversion of 1-hexene using HTS-1#S0.25 as the catalyst is much higher than that using hollow TS-1 prepared by the conventional method as the catalyst. HTS-1#S0.25 remains a high oxidation activity and high selectivity after five recycle experiments. In addition, the type of anion also has a great influence on the reactivity. This method can provide a new idea for the preparation of hollow TS-1.
研究领域:
材料化学
·
催化科学
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产品关联
DOI
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2014
·
IF 2.045
Guo-Min Yu,Li-Sheng Wang,Chao-Jun Du
A phosphorus flame retardant of 4-(hydroxymethyl)-1-oxido-2,6,7-trioxa-1-phosphabicyclo[2.2.2]octane (PEPA) was synthesized by pentaerythritol and phosphorus oxychloride at a certain condition. The structural features of PEPA were investigated with Fourier transform infrared spectroscopy and 1H NMR methods. The thermal behavior of PEPA was carried out with differential scanning calorimetry and thermogravimetric analysis instruments. A static analytic method was used to measure the solubilities of the PEPA in several organic solvents. The measurements were accurately acted in ethyl acetate, acetonitrile, isopropyl alcohol, ethanol, n-propyl alcohol, acetone, methanol, and chloroform at the temperature range from 292.75 K to 333.45 K. Using the Scatchard–Hildebrand methodology, the activity coefficients of the solute have been calculated, and the partial molar excess enthalpies at infinite dilution were also predicted.
研究领域:
化学
·
材料科学
·
物理化学
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产品关联
DOI
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2021
·
IF 2.694
Lixia Yang,Yuexia Han,Zhen Jiao
To obtain the basic data for the recovery of 1-methylimidazole from wastewater by extraction, the ternary liquid–liquid phase equilibrium data of the water (1) + 1-methylimidazole (2) + 1-butyl-3-methylimidazolium hexafluorophosphate ([Bmim][PF6]) (3) system were determined using an equilibrium autoclave at the temperatures of 303.15 to 323.15 K and 101.32 kPa. The solute distribution coefficient (D) and the selectivity (S) of the ternary system were calculated in accordance with the experimental data. Their values were up to 1.39 and 41.17, respectively, which showed that the ionic liquid can extract 1-methylimidazole effectively. The experimental results showed that the temperature had a slight effect on the liquid–liquid equilibrium behavior. Meanwhile, the calculated results revealed that the selectivity coefficient decreased with the enhancing concentration of 1-methylimidazole in the ternary system and the increasing temperature. In addition, the nonrandom two liquid (NRTL) model was used for correlation of the ternary systems. The root-mean-square deviation value of 0.0218 indicated that the calculated results using the model were in good agreement with the experimental data and were reliable to apply to practice.
研究领域:
化学工程
·
环境科学
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DOI
高影响因子
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