Acta Biomaterialia
·
2023
·
IF 9.7
Yinghui Xu,Jiayi Bian,Xin Liu,Zhengzheng Qian,Minghao Sun,Cheng Zhang,Ruiyang Pan,Qitong Li,Changrui Sun,Bin Lin,Kun Peng,Nan Lu,Xikuang Yao,Wenpei Fan
Photothermal therapy (PTT) combined with chemodynamic therapy (CDT) presents an appealing complementary anti-tumor strategy, wherein PTT accelerates the production of reactive oxygen species (ROS) in CDT and CDT eliminates residual tumor tissues that survive from PTT treatment. However, nanomaterials utilized in PTT/CDT are limited by non-specific damage to the entire organism. Herein, a glucose-responsive enzymatic Fe@HRP-ABTS/GOx nanodot is judiciously designed for tumor-specific PTT/CDT via a simple and clean protein-templated biomimetic mineralization synthesis. By oxidizing glucose in tumor cells, glucose oxidase (GOx) activates glucose-responsive tumor therapy and increases the concentration of H 2 O 2 at the tumor site. More importantly, the self-supplied peroxide hydrogen (H 2 O 2 ) can convert ABTS (2,2′-Hydrazine-bis(3-ethylbenzothiazoline-6-sulfonic acid) diamine salt) into oxidized ABTS (oxABTS) through horseradish peroxidase (HRP) catalysis for PTT and photoacoustic (PA) imaging. Furthermore, the Fe 2+ arising from the reduction of Fe 3+ by overexpressed GSH reacts with H 2 O 2 to generate intensely reactive •OH through the Fenton reaction, concurrently depleting GSH and inducing efficient tumor CDT. The in vitro and in vivo experiments demonstrate superior cancer cell killing and tumor eradication effect of Fe@HRP-ABTS/GOx nanodot under near-infrared (NIR) laser irradiation. Collectively, the nanodots provide mutually reinforcing catalytic PTT/CDT anti-tumor strategies for treating liver cancer and potentially other malignancies. Statement of significance Combinatorial antitumor therapy with nanomedicines presents great prospects for development. However, the limitation of non-specific damage to normal tissues hinders its further clinical application. In this work, we fabricated tumor-selective biomimetic Fe@HRP-ABTS/GOx nanodots for H 2 O 2 self-supplied catalytic photothermal/chemodynamic therapy of tumors. The biomimetic synthesis strategy provides the nanodots with enzymatic activity in response to glucose to produce H 2 O 2 . The self-supplied H 2 O 2 initiates photothermal therapy with oxidized ABTS and enhances chemodynamic therapy through simultaneous •OH generation and GSH depletion. Our work provides a new paradigm for developing tumor-selective catalytic nanomedicines and will guide further clinical translation of the enzymatic biomimetic synthesis strategy.
研究领域:
纳米技术
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癌症治疗
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生物医学工程
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材料科学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Jiaxi Deng,Yiyao Luo,Jia Zheng,Zirui Yuan,Yi Ma,Huibo Luo,Xiaogang Luo,Danqun Huo,Changjun Hou
Baijiu represents the pinnacle of traditional Chinese culinary culture. The influence of Baijiu is evident in various literary works, rituals and banquets, dishes and healthcare, and numerous other areas. The reduction of substances plays a pivotal role in the flavor, aroma, and quality control of Baijiu. Therefore, the present work focuses on Baijiu and the reducing substances in it. In this study, CDs@ZrFe-MOF and three distinct catalase nanase substrates (ABTS, OPD, and TMB) were employed to create fluorescence and colorimetric dual-mode sensor arrays. The oxidation of the enzyme substrate into the appropriate oxidation substrate is facilitated by CDs@MOF in the presence of H₂O₂. The reducing substance effectively inhibits the formation of the oxidation substrate, resulting in alterations in fluorescence and UV intensity. The dual-mode sensor array was found to be effective in detecting 15 different types of reducing chemicals. Furthermore, the colorimetric and fluorescence sensor array demonstrated exceptional detection capability in detecting 19 different types of Luzhou-flavor liquor samples, achieving a 100% accuracy rate. This study will provide an accurate and rapid method for differentiating Baijiu.
研究领域:
分析化学
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纳米技术
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传感器技术
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食品科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Hao Wu,Yaling Liu,Hongyong Wang,Jun Wu,Feifan Zhu,Pei Zou
In this study, a simple, label-free, and enzyme-free colorimetric biosensor has been developed for amplified detection of let-7a microRNA (miRNA) on the basis of dual signal amplification strategy. The sensing system mainly consists of four unlabeled hairpin probes termed H1, H2, H3, and H4. Upon sensing of the target miRNA, hairpin H1 is opened. Then hairpin H2 hybridizes with H1 forming H1–H2 duplex and frees the target miRNA that can be recycled to trigger another reaction cycle. In addition, the newly formed H1–H2 duplex hybridizes with hairpin H3, and this triggers the autonomous cross-opening of the two hairpins H3 and H4 through hybridization chain reaction. During this process, numerous split G-quadruplex structures are generated and further associate with cofactor hemin to form massive peroxidase-mimicking DNAzymes. The resulting DNAzymes catalyze the H 2 O 2 -mediated oxidation of colorless 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS 2− ) to the green-colored ABTS •− , inducing a remarkably amplified colorimetric signal. This newly developed sensing system exhibits high sensitivity toward miRNA with a detection limit of 7.4 fM and a large dynamic range of 6 orders of magnitude from 10 fM to 10 nM. Furthermore, it exhibits a good performance to discriminate single-base difference among the miRNA family members and holds a great potential for early diagnosis in gene-related diseases.
研究领域:
生物化学
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分子生物学
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分析化学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
·
2025
·
IF 8.2
Xiaoming Song,Dan Feng,Jiamin Li,Liyan Zang,Hongmei Li,Jing Sun
This study integrates HPLC fingerprinting and NIR spectroscopy to evaluate the antioxidant capacity of Rheum tanguticum . HPLC fingerprinting comprehensively characterized the complex chemical composition, identifying 13 compounds. Meanwhile, NIR spectroscopy provided a rapid, non-destructive approach to predict antioxidant capacity. The combination of these techniques offers a holistic and efficient strategy for evaluating antioxidant activity. The spectrum-effect relationships were analyzed by correlating the results of three antioxidant assays (ABTS, DPPH, and FRAP) with the peak areas of the common peaks identified by HPLC. Glycosylated anthraquinones, particularly conjugated forms like rhein-8-O-glucoside, were found to synergistically enhance antioxidant activity with free aglycones. NIR models optimized using the PLS method with Python-based algorithms demonstrated strong predictive abilities, achieving RPD values of 2.43 for ABTS, 2.63 for DPPH, and 2.43 for FRAP. This research provides a rapid method to evaluate the antioxidant capacity of Rh. tanguticum , offering a reference for future applications in other fields.
研究领域:
药物分析
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光谱学
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色谱学
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药物化学
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计算生物学
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植物科学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Shang Gao,Tiantian Li,Zhao-Rong Li,Bingwu Liao,Mouming Zhao,Chunxia Zhou,Donghui Luo,Rui-Bo Jia
Coreopsis tinctoria buds grow at high altitudes and have unique medicinal and health effects. This study focused on the chemical components of gradient ethanol extracts of Coreopsis tinctoria buds and their impact on the gut microbiota and its metabolites. UPLC-Q-Exactive-MS results disclosed the presence of 24 distinct chemicals in the extracts, marein, and quercetin-3- β -D-glucoside as the predominant antioxidants. Furthermore, the anhydrous ethanol extract (EtOH-EX) and 80 % ( w /w) ethanol extract (80 % EtOH-EX) showed the highest 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis-(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) free radical scavenging capacity, and the water extract (Water-Ex) showed excellent bile acid binding capacity. Besides, simulated fermentation of the extracts increased short-chain fatty acids and the abundance of gut-friendly bacteria like Bifidobacteria , especially in the Water-EX group. Correlation analysis revealed that the key microbiota on metabolites production. In conclusion, the extracts derived from Coreopsis tinctoria buds exhibit a spectrum of bioactivities, viewing them as flexible options for applications across food and pharmaceutical products.
研究领域:
药理学
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食品科学
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微生物学
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分析化学
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产品关联
DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
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2018
·
IF 2.616
You Luo,Bin Peng,Yan Liu,Yanan Wu,Zhenqiang Wu
Plant polysaccharides possess multiple physiological activities. Box-Behnken design (BBD) was employed to optimize the ultrasound-assisted extraction of polysaccharides from guava leaves (GLP). It was found that ultrasonic time, temperature and power has a significant effect on the extraction efficiency. The yield of GLP was 1.00 ± 0.04% under the optimal condition of extraction time of 20 min, temperature of 62 ℃ and ultrasound power of 404 W. The DPPH and ABTS + radical scavenging rate of GLP was 56.38% and 51.73% at 100 μg/mL, respectively. The rate of inhibition of α-amylase by GLP was only 14.06% at 1 mg/mL; however, its α-glucosidase inhibition rate reached 99.54% at 100 μg/mL. These results suggested that the ultrasound-assisted extraction for GLP is effective and environmentally friendly and GLP has wide application potentials as natural antioxidants and α-glucosidase inhibitors.
研究领域:
药理学
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食品科学
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植物科学
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产品关联
DOI
高影响因子
JOURNAL OF FOOD PROCESSING AND PRESERVATION
·
2019
·Vol. 43
·期 12
·e14277
·
IF 1.288
Xiao Gong,Xiaobing Huang,Taohua Yang,Jing Wen,Wei Zhou,Jihua Li
The effects of hot air drying (HD), freeze-drying (FD), microwave drying (MWD), natural convective drying (NCD), and vacuum drying (VD) on the physicochemical, nutritional, and antioxidant properties of okra pods were investigated. Changes in the surface morphology, color, rehydration ratio, protein, pectin, ascorbic acid, and phenolics, as well as antioxidant activities of okra pods measured by 2,2′-azinobis (3-ethylbenzo thiazoline-6-sulphonic acid) diammonium salt (ABTS), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and Ferric Ion Reducing Antioxidant Power (FRAP) assays were evaluated. In general, FD products exhibited a better retention of the texture characteristics, nutritional, and antioxidant activities, and was suitable for okra pods drying, although FD was the longer time-consuming method among the five drying methods. Furthermore, NCD products were poorer in color, texture, and nutrition. Future studies should continuously focus on various combinations of different types of drying methods to supply okra pods products with the most affordable cost and the highest quality. Practical applications Physicochemical properties and antioxidant capacities of okra pods are affected by the drying process. The drying process with nonthermal, low temperature, and low content of oxygen is beneficial to the retention of nutrients and functional components. In general, freeze-drying exhibited a better texture characteristics and good antioxidant activities, although higher energy and longer time consumption was the current challenge. How to combine the different types of drying methods has become an inevitable trend and research focus.
研究领域:
食品科学
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农业工程
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营养学
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产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2015
·
IF 2.837
Dong-Yang Zhang,Xiao-Hui Yao,Ming-Hui Duan,Fu-Yao Wei,Guo-Hua Wu,Long Li
Flos Lonicerae (FL) was widely planted in China as a kind of pharmaceutical resource. In our research, the variations in content and antioxidant activity of essential oil of FL from five different sites of China were investigated. The results revealed that the essential oil content of samples varied over the range of 0.288 ± 0.015% to 0.387 ± 0.016%. The essential oil antioxidant activity of samples were also determined by scavenging activity against 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid ammonium salt) (ABTS), ferric reducing/antioxidant power (FRAP) test and β-carotene-linoleic acid bleaching test. The results indicated that the sample from Shaoyang showed better antioxidant activity than the others. And the β-carotene-linoleic acid bleaching activity could reach 92.31%, when the concentration of the sample from Shaoyang was 4 mg/mL, indicating that the sample from Shaoyang possessed excellent antioxidant activity. The chemical compositions of the essential oil were finally identified by GC–MS, and more than 20 compounds were identified. Principal component analysis was also performed to investigate the difference of the essential oil samples.
研究领域:
药理学
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植物学
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分析化学
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2013
·
IF 5.437
Yanli Hao,Qingquan Guo,Hanyin Wu,Liangqia Guo,Liangshuang Zhong,Jing Wang,Tianran Lin,FengFu Fu,Guonan Chen
An amplified colorimetric detection of Hg 2+ is proposed by combining T–Hg 2+ –T base pairs and hybridization chain reaction (HCR). Two hairpins consisting of three-fourths and one-fourth of the horseradish peroxidase (HRP)-mimicking DNAzyme in inactive configuration are used as functional elements. In the presence of Hg 2+ , one of the hairpins is opened by an assistant probe with the help of T–Hg 2+ –T base pairs and this triggers an autonomous cross-opening of the two hairpins using the strand displacement principle, resulting in the formation of DNA nanowires consisting of numerous reunited Q-quadruplex DNAzyme units. The resulting catalytically active hemin/G-quadruplex HRP-mimicking DNAzymes catalyze the oxidation of ABTS 2− by H 2 O 2 into a green-colored cationic radical ABTS •+ for the colorimetric readout. This “turn-on” sensing system enables the high sensitive and selective detection of aqueous Hg 2+ with a detection limit of 9.7 pM.
研究领域:
分析化学
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纳米技术
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环境科学
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Chengyang Ban,Haimeng Liu,Yaoyu Zhang,Qiong Ding,Changzhi Xu,Jibin Miao,Ru Xia,Jiasheng Qian,Ming Cao
Photothermal therapy (PTT) holds great promise for the treatment of bacterial infections, but the traditional “always-on” photothermal agents severely restrict further clinical applications. Here, a pH-activated off/on PTT/CDT nanosystem for synergistic antibacterial/antibiofilm application was reported. Using mesoporous silica as a nanocarrier, such activatable photoagent was achieved via specific redox reactions between 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and Fe(III), which could switch PTT/CDT from “off” to “on” in response to pH. Under neutral conditions, the nanosystem was “off state” due to the isolation of Fe(III) and ABTS. Whereas under acidic conditions, the nanosystem selectively activated to “on state”. The released Fe(III) can oxidize ABTS to photothermal reagent of ABTS •+ , and itself was reduced to Fe(III)/Fe(II) pairs. The Fe(II)/Fe(III) pairs can catalyze the H 2 O 2 to hydroxyl radicals (•OH) via Fenton reactions for chemodynamic therapy (CDT). The in vitro results showed that the pH-activated off/on PTT/CDT nanosystem have an excellent antibacterial efficiency (˃90 %) and enhanced antibiofilm activity for Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ).
研究领域:
纳米技术
·
材料科学
·
生物医学工程
·
微生物学
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产品关联
DOI
高影响因子
Foods
·
2024
·Vol. 13
·期 8
·1158
·
IF 4.7
Yuzhu Zhao,Doudou Liu,Jiaxuan Zhang,Jiaxin Shen,Jiamin Cao,Huawei Gu,Mengqing Cui,Ling He,Gong Chen,Shuwen Liu,Kan Shi
Grape pomace seeds contain abundant phenolic compounds, which are also present in both soluble and insoluble forms, similar to many other plant matrices. To further increase the extractable soluble phenolics and their antioxidant activities, grape pomace seeds were fermented with different fungi. Results showed that solid-state fermentation (SSF) withAspergillus niger,Monascus anka, andEurotium cristatumat 28 °C and 65% humidity had a significantly positive impact on the release of soluble phenolics in grape pomace seeds. Specifically, SSF withM. ankaincreased the soluble phenolic contents by 6.42 times (calculated as total phenolic content) and 6.68 times (calculated as total flavonoid content), leading to an overall improvement of antioxidant activities, including DPPH (increased by 2.14 times) and ABTS (increased by 3.64 times) radical scavenging activity. Furthermore, substantial changes were observed in the composition and content of individual phenolic compounds in the soluble fraction, with significantly heightened levels of specific phenolics such as chlorogenic acid, syringic acid, ferulic acid, epicatechin gallate, and resveratrol. Notably, duringM. ankaSSF, positive correlations were identified between the soluble phenolic content and hydrolase activities. In particular, there is a strong positive correlation between glycosidase and soluble phenols (r = 0.900). The findings present an effective strategy for improving the soluble phenolic profiles and bioactivities of grape pomace seeds through fungal SSF, thereby facilitating the valorization of winemaking by-products.
研究领域:
食品科学
·
生物技术
·
化学
·
农业
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2020
·
IF 4.389
Ping Xue,Xueping Liu,Yaohua Gu,Weiwei Zhang,Lan Ma,Rui Li
A heterogeneous bead-shaped biocatalyst was prepared by assembling co-immobilization of encapsulating mediator 2,2′-Azino-bis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) into the functionalized calcium alginate composite beads by grafting glycidyl methacrylate and dopamine and then immobilizing laccase covalently onto beads. The enzyme-catalyzed degradation for acridine in water was systematically studied for the first time, and it was found that the co-immobilized laccase-mediator system has excellent capability to degrade acridine in water. Treating 30 mg/L acridine aqueous solution for 8 h with the biocatalyst, the degradation rate of acridine reached 100 %. In contrast, the degradation of acridine by free laccase under the same treatment conditions was low and difficult to observe. Its outstanding catalytic activity could be attributed to the effective assembling co-immobilization of laccase and the mediator so that the catalytic activity of the enzyme and the synergistic promoting action of the mediator have been given full play in the process of acridine degradation. The beaded biocatalyst about 3 mm in diameter could be easily recovered and reused several times without obvious deterioration in catalytic activity. Furthermore, according to the test results of electron paramagnetic resonance and the identified intermediates, the possible biocatalytic mechanism and reaction pathways for acridine degradation have been proposed.
研究领域:
环境科学
·
生物技术
·
催化化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）