JOURNAL OF APPLIED ENTOMOLOGY
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2020
·Vol. 144
·期 8
·732-742
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IF 2.211
Xiaoyun Ren,Shengyong Wu,Zhenlong Xing,Yulin Gao,Wanzhi Cai,Zhongren Lei
Western flower thrips (WFTs), Frankliniella occidentalis Pergrande, and onion thrips (OTs), Thrips tabaci Lindeman, are two cosmopolitan insect pests of agricultural and horticultural plants. Understanding the occurrence and development of thrips on plants is crucial for identifying suitable plants that can be used for developing a “push-pull” strategy against thrips. In this study, the dynamics of WFTs and OTs on plants ( Allium fistulosum L., Medicago sativa L., Luffa cylindrica (L.) Roem., Ocimum basilicum L., and Schizonepeta tenuifolia (Benth.) Briq.) were investigated for two consecutive years (2018–2019). Throughout the survey, the abundances of both thrips species were strongly associated with plant species and plant phenology; both thrips species were present at relatively high densities on M. sativa but very low densities on O. basilicum and S. tenuifolia . Populations of both thrips species greatly increased during plant flowering. A Y-tube olfactory test was used to study the effects of plant volatiles in mediating thrips behaviour and showed that volatiles of M. sativa were attractive to both thrips species whether emitted by the plant in the vegetative or flowering stage, while volatiles of O. basilicum and S. tenuifolia were repellent to thrips. Additionally, because of the presence of a high number of floral chemicals, both thrips species exhibited a greater preference for volatiles emitted by plants in the flowering period over those emitted by plants in the vegetative period. Our observations indicate that plant species and flowering status play an important role in the abundance dynamics of thrips and that the volatiles of flowering plants attract thrips more strongly than volatiles emitted by vegetative plants. These findings can facilitate the screening of attractive/unattractive plants for developing push-pull strategies to control thrips.
研究领域:
昆虫学
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植物生理学
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害虫管理
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DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2018
·
IF 5.091
Kui Yin,Shanshan Zhang,Yuyang Sun,Hui Huang,Yafei Cheng,Fan Liao,Mingwang Shao
Hydrogen is considered as a promising energy source because of its high combustion heat and clean products. Among the various hydrogen evolution methods, electrocatalysis is the most convenient and effective way. However it is still problematic to find low-cost and high-efficiency electrocatalysts. In this work, Os-Ag-Si and Os-Au-Si ternary electrocatalysts were investigated. It is interesting that Os-Ag-Si exhibits higher hydrogen evolution performance than that of Os-Au-Si, even though Ag is a worse HER catalyst than Au. The optimal ternary catalyst Os-Ag-Si with mass ratio of 15:15:70 (Os:Ag:Si) was synthesized by a facile Si H bond reduction method, which shows superior hydrogen evolution reaction performance with a low Tafel slope of 40 mV·dec −1 . In addition, the current density of Os-Ag-Si-15 catalyst surpasses that of 20% Pt/C when the overpotential is larger than 160 mV. The introduction of Ag in the Os-Ag-Si ternary catalyst could enhance the electrocatalytic activity, reduce the cost of catalysts, and even be better than Os-Au-Si.
研究领域:
材料科学
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能源化学
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DOI
PubMed
高影响因子
ACS Chemical Neuroscience
·
2019
·
IF 3.861
Hanbo Pan,Hongda Qiu,Ke Zhang,Panpan Zhang,Weida Liang,Mengxiang Yang,Chenye Mou,Miaoman Lin,Ming He,Xiao Xiao,Difan Zhang,Haixing Wang,Fufeng Liu,Yongmei Li,Haixiao Jin,Xiaojun Yan,Hongze Liang,Wei Cui
Alzheimer’s disease (AD) is characterized by progressive neurodegeneration and impaired cognitive functions. Fascaplysin is a β-carboline alkaloid isolated from marine sponge Fascaplysinopsis bergquist in 1988. Previous studies have shown that fascaplysin might act on acetylcholinesterase and β-amyloid (Aβ) to produce anti-AD properties. In this study, a series of fascaplysin derivatives were synthesized. The cholinesterase inhibition activities, the neuronal protective effects, and the toxicities of these compounds were evaluated in vitro. Compounds 2a and 2b, the two most powerful compounds in vitro, were further selected to evaluate their cognitive-enhancing effects in animals. Both 2a and 2b could ameliorate cognitive dysfunction induced by scopolamine or Aβ oligomers without affecting locomotor functions in mice. We also found that 2a and 2b could prevent cholinergic dysfunctions, decrease pro-inflammatory cytokine expression, and inhibit Aβ-induced tau hyperphosphorylation in vivo. Most importantly, pharmacodynamics studies suggested that 2b could penetrate the blood–brain barrier and be retained in the central nervous system. All these results suggested that fascaplysin derivatives are potent multitarget agents against AD and might be clinical useful for AD treatment.
研究领域:
神经科学
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药理学
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药物化学
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DOI
PubMed
高影响因子
NEUROCHEMICAL RESEARCH
·
2018
·Vol. 43
·期 4
·796-805
·
IF 2.772
Song Xiaoyu,Liu Bo,Cui Lingyu,Zhou Biao,Liu Lu,Liu Weiwei,Yao Guodong,Xia Mingyu,Hayashi Toshihiko,Hattori Shunji,Ushiki-Kaku Yuko,Tashiro Shin-ichi,Ikejima Takashi
Alzheimer’s disease (AD) is a progressive neurodegenerative disease that is characterized by a cascade of pathologic changes. A widely discussed theory indicates that amyloid β (Aβ) peptides are the causative agents of AD. Silibinin, a flavonoid derived from milk thistle, is well known for its hepato-protective activities and we have reported the neuroprotective effects of silibinin. In this study, we investigated the role of estrogen receptors (ERs) in silibinin’s neuroprotective effect on Aβ 1−42 -injected rats. Results of Morris water maze and novel object-recognition tests demonstrated that silibinin significantly attenuated Aβ 1−42 -induced memory impairment. Silibinin attenuated ERs and PI3K-Akt pathways, as well as modulated mitogen-activated protein kinases in the hippocampus of Aβ 1−42 -injected rats. Taken together, silibinin is a potential candidate in the treatment of Alzheimer’s disease.
研究领域:
神经科学
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药理学
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DOI
PubMed
高影响因子
NEUROSCIENCE
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2018
·
IF 3.382
Lingqi Gao,Junde Han,Jie Bai,Jing Dong,Sen Zhang,Mazhong Zhang,Jijian Zheng
Early synchronized spontaneous network activity is a hallmark of the brain growth spurt period, during which general anesthetics cause widespread neuronal apoptosis and subsequent cognitive dysfunction. However, the relationship of such activity to anesthetic-induced neuronal apoptosis remains to be determined. In this study, we utilized patch-clamp electrophysiological recording, immunohistochemistry, and TUNEL assays to investigate the potential roles of spontaneous network activity in ketamine-induced neuronal apoptosis during early development. All experiments were performed using acutely dissected whole-mount Sprague–Dawley rat retinas (0–14 postnatal days [P0–P14]). Ketamine reversibly blocked spontaneous network activity in the rat retina from P0 to P9 and irreversibly blocked such activity from P10 to P12. The peak of physiological and ketamine-induced neuronal apoptosis mainly occurred from P7 to P9. Blockade of nicotinic acetylcholine receptors (nAChRs) also induced reversible inhibition of spontaneous network activity from P0 to P7 and extensive neuronal apoptosis in the P7 rat retina, while activation of nAChRs or increases in endogenous ACh levels attenuated ketamine-induced apoptotic responses. Furthermore, blockade of α7-nAChR and ß2-nAChR subtypes induced neuronal apoptosis in the developing retina, while activation of the α7-nAChR subtype attenuated ketamine-induced apoptotic responses. These results demonstrate that ketamine may inhibit early synchronized spontaneous network activity by blocking nAChRs, and that such inhibition may contribute to ketamine-induced neuronal apoptosis in the developing rat retina.
研究领域:
神经科学
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药理学
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发育生物学
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
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2021
·
IF 4.24
Yao Pan,Hongyan Li,Xuan Chen,Yawei Fan,Bing Zhang,Rong Liu,Ze-yuan Deng
Lipophilic (lycopene, β-carotene) and hydrophilic (caffeic acid, p -coumaric acid) phytochemicals were mixed in molar mass at different concentrations/ratios to explore the antioxidant interactions on H 2 O 2 -treated H9c2 cells. Reactive oxide species (ROS), expression of antioxidant enzymes , and oxidation products were analyzed and evaluated by synergistic rate (SR) and combination index (CI). At constant concentrations, antioxidant interactions were influenced by ratios. Group contained more hydrophilic compounds (eg., p -coumaric acid:β-carotene=9:1) showed antioxidant synergism (SR=22.33±1.23, SR>0) at 3.0 μM, while groups that contained more lipophilic compounds (eg., p -coumaric acid:β-carotene=1:9) showed antioxidant antagonism (SR=-11.68±1.25, SR<0) at 3.0 μM. Antioxidant interactions were also influenced by concentrations. From results, ROS generation of group ( p -coumaric acid:β-carotene=1:9) changed from synergism (SR=12.27±2.34, SR>0) to antagonism (SR=-11.68±1.25, SR<0) as their total concentrations increased from 1.5 μM to 3.0 μM. Therefore, this study discussed the possible mechanism of ratio and concentration on the antioxidant interaction among hydrophilic and lipophilic phytochemicals. It also suggested that proper concentrations/ratios of hydrophilic and lipophilic phytochemicals should be considered in diet and formulation of functional foods.
研究领域:
药理学
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营养学
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生物化学
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DOI
高影响因子
Insects
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2021
·Vol. 12
·期 7
·651
·
IF 2.769
Xiaorong Xing,Mengwen Yan,Huilin Pang,Fu’an Wu,Jun Wang,Sheng Sheng
Simple SummaryP450s are involved in the detoxification process in insects; however, less attention has been paid for their roles in parasitoid wasps. Here, we identified 28 P450s from the previous constructed transcriptome dataset ofMeteorus pulchricornisand validated their expression patterns under insecticides exposure. Furthermore, we silencedCYP369B3in adult wasps and demonstrated that the knocking downCYP369B3significantly increased of the mortality of adult wasps when they were exposed to each chemical insecticide, phoxim, cypermethrin, and chlorfenapyr, respectively. The present study provides a theoretical base for functional research on P450 genes in parasitoid wasps.AbstractWith the widespread application of insecticides, parasitoid wasps may also be under risk when exposed to insecticides directly at their free-living stages. The endoparasitoid waspMeteorus pulchricornisis the predominant natural enemy of many lepidopteran pests, such asSpodoptera lituraandHelicoverpa armigera. The cytochrome P450 monooxygenases constitute a ubiquitous and complex superfamily of hydrophobic, haem-containing enzymes. P450s are involved in the detoxification of many xenobiotics. However, their exact roles in the tolerance mechanism in parasitoids toward insecticides has received less attention. Here, 28 P450 genes inM. pulchricorniswere identified from a previously constructed transcriptome dataset. These P450 genes belonged to CYP2, -3, and -4, and mitochondrial clans. Subsequently, eight candidateMpulCYPswere selected from four CYP clans to validate their expression patterns under phoxim, cypermethrin, and chlorfenapyr exposure by qRT-PCR. The results showed that all three insecticides had significant effects on the expression ofMpulCYPs. To further study the function of P450s,CYP369B3was silenced, and its expression levels ofCYP369B3were significantly decreased. Survival analysis indicated that after dsRNA injection, the mortality rate of wasps was significantly increased whenM. pulchricornisfemales were exposed to insecticides compared to control groups. Our findings provide a theoretical base for elucidating the mechanism of insecticide tolerance and promote functional research on P450 genes in parasitoid wasps.Keywords:Meteorus pulchricornis;cytochrome P450s;detoxification;insecticide tolerance
研究领域:
昆虫学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
TOXICOLOGY LETTERS
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2021
·
IF 4.374
Jinwen Yuan,Xiaoming Sun,Siyan Che,Li Zhang,Zheng Ruan,Xiaomin Li,Junhua Yang
Polybrominated diphenyl ethers (PBDEs) are persistent organic pollutants. They are constantly detected in terrestrial, ocean, and atmospheric systems, and it is of particular concern that these fat-soluble xenobiotics may have a negative impact on human health. This study aimed to evaluate the toxic effect and underlying mechanism of decabromodiphenyl ether (BDE-209) on human liver in a HepG2 cell model. The results showed that BDE-209 significantly induced HepG2 cells apoptosis, increased intracellular reactive oxygen species (ROS), disturbed [Ca 2+ ] homeostasis and mitochondrial membrane potential (MMP), and caused nuclear shrinkage and DNA double-strand breaks. BDE-209 also significantly decreased the activities of antioxidant parameters, superoxide dismutase (SOD), total antioxygenic capacity (T-AOC), glutathione (GSH), and total glutathione (T-GSH). The up-regulation of the Aryl hydrocarbon receptor (AhR)/cytochrome P4501A1 (CYP1A1) signaling pathway indicates that after long-term and high-dose exposure, BDE-209 may be a liver carcinogen. Interestingly, HepG2 cells attempt to metabolize BDE-209 through the Nrf2-mediated antioxidant pathway. These findings help elucidate the mechanisms of BDE-209-induced hepatotoxicity in humans.
研究领域:
毒理学
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环境科学
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细胞生物学
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分子生物学
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DOI
PubMed
高影响因子
Food Chemistry-X
·
2023
·
IF 6.1
Chang He,Jingtao Zhou,Yuchuan Li,De zhang,Bernard Ntezimana,Junyu Zhu,Xiaoyong Wang,Wenluan Xu,Xiaoju Wen,Yuqiong Chen,Zhi Yu,Yu Wang,Dejiang Ni
This study delved into the aroma characteristics of “Qingxiang” oolong tea, analyzing six different cultivars and their processing modes. The findings showed that both cultivars and processing modes have a significant impact on the oolong tea aroma system. The study identified 18 terpenoid volatiles (VTs), 11 amino-acid-derived volatiles (AADVs), 15 fatty-acid-derived volatiles (FADVs), 3 carotenoid-derived volatiles (CDVs), and 10 other compounds in oolong tea that differentiate it from green and black tea. The turn-over stage was found to be the primary processing stage for oolong tea aroma formation. Molecular sensory analysis revealed that the “fresh” odor attribute is the basis for its aroma, while “floral and fruity” fragrances are its aroma characteristics. The perception of oolong tea as “fresh” and “floral and fruity” is influenced by the interactions of its aroma components. These findings provide a new basis for breed improvement and process enhancement in oolong tea production.
研究领域:
食品科学
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农学
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化学
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感官科学
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产品关联
DOI
PubMed
高影响因子
Journal of Environmental Sciences
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2022
·
IF 6.796
Zhihui Lv,Guangzhi He,Wenshuo Zhang,Jingjing Liu,Zhihua Lian,Yang Yang,Zidi Yan,Guangyan Xu,Wenpo Shan,Yunbo Yu,Hong He
TiO 2 -supported V 2 O 5 catalysts are commonly used in NO x reduction with ammonia due to their robust catalytic performance. Over these catalysts, it is generally considered that the active species are mainly derived from the vanadia species rather than the intrinsic structure of V-O-Ti entities, namely the interface sites. To reveal the role of V-O-Ti entities in NH 3 -SCR, herein, we prepared TiO 2 /V 2 O 5 catalysts and demonstrated that V-O-Ti entities were more active for NO x reduction under wet conditions than the V sites (V=O) working alone. On the V-O-Ti entities, kinetic measurements and first principles calculations revealed that NH 3 activation exhibited a much lower energy barrier than that on V=O sites. Under wet conditions, the V-O-Ti interface significantly inhibited the transformation of V=O to V-OH sites thus benefiting NH 3 activation. Under wet conditions, meanwhile, the migration of NH 4 + from Ti site neighboring the V-O-Ti interface to Ti site of the V-O-Ti interface was exothermic; thus, V-O-Ti entities together with neighboring Ti sites could serve as channels linking NH 3 pool and active centers for activation of NH 4 + . This finding reveals that the V-O-Ti interface sites on V-based catalysts play a crucial role in NO x removal under realistic conditions, providing a new perspective on NH 3 -SCR mechanism.
研究领域:
催化化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2023
·Vol. 79
·期 8
·2713-2724
·
IF 4.1
Lang Ming,Yuan-Wen Du,Ge-Ge Yuan,Qi Su,Xiao-Bing Shi,Huan Yu,Gong Chen
Background Herbivore-induced plant volatiles (HIPVs) are important self-defense outputs of pepper plants to resist insect pests. Ascoviruses are pathogenic to the larvae of most lepidopteran vegetable pests. However, whether Heliothis virescens ascovirus 3h (HvAV-3h)-infected Spodoptera litura larvae can change pepper leaf HIPVs is not well understood. RESULTS Spodoptera litura larvae preferred S. litura -infested leaves, and this preference was stronger with longer duration of S. litura infestation. In addition, S. litura larvae significantly chose pepper leaves damaged by HvAV-3h-infected S. litura over the healthy pepper leaves. Results also showed that S. litura larvae preferred leaves mechanically damaged and treated with oral secretions from HvAV-3h infected- S. litura larvae in a simulation test. We captured the volatiles emitted by leaves under six treatments. Results showed that the volatile profile changed with the different treatments. Testing of volatile blends, prepared to the proportion released showed that the blend from simulated HvAV-3h-infected S. litura larvae-damaged plants was the most attractive to S. litura larvae. Further, we also found that some of the compounds significantly attracted S. litura larvae at specific concentrations. CONCLUSION HvAV-3h-infected S. litura can alter the release of HIPVs in pepper plants and thus become more attractive to S. litura larvae. We speculate that this may be due to alterations in the concentration of some compounds (such as geranylacetone and prohydrojasmon) affecting the behavior of S. litura larvae. © 2023 Society of Chemical Industry.
研究领域:
植物学
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昆虫学
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生态学
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DOI
PubMed
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
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2023
·
IF 5
Lixue Ma,Zhaohua Li,Jiancheng Fei,Mo Li,Bingyang Liu,Baocheng Tian,Yanhua Liu,Qiang Fu
Nanocrystals (NCs) are considered as an effective strategy for the formulation of intravenous injections for poorly water-soluble drugs, despite they are metastable dispersions. Typically, stabilizers are essential for the maintenance of physical stability, and they are important compositions for NCs. However, whether the stabilizers have impacts on the in vivo behaviors of NCs after intravenous injection are unknown. In this study, three meloxicam NCs (MLXNCs) were each formulated with a different steric stabilizer (PEG 400, lecithin and PVP-K17). The developed MLXNC PEG , MLXNC Lec and MLXNC PVP all had particle size of about 250 nm and shared the same crystal form. All three formulations had similar pharmacokinetic profiles and therapeutic efficacy, with no statistically significant differences. Overall results provided evidence that stabilizers have negligible effects on the in vivo behaviors of intravenous NCs and stress the importance of physical stability for formulation development.
研究领域:
药剂学
·
药物代谢动力学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）