TECHNOLOGY IN CANCER RESEARCH & TREATMENT
·
2024
·
IF 2.7
Li-Hong Liu,Yu-Feng Liu,Hong-Bo Zhang,Xiao-Lei Liu,Han-Wen Zhang,Biao Huang,Fan Lin,Wei-Hua Li
PurposeDevelop an albumin nanoparticle-based nanoprobe for targeted glioblastoma (GBM) diagnosis and treatment, utilizing Angopep-2 for low-density lipoprotein receptor-related protein (LRP) targeting.MethodsCombined albumin-coated superparamagnetic iron oxide (SPIO), Carmustine (BCNU), and indocyanine green (ICG). Assessed morphology, size, Zeta potential, fluorescence, and drug encapsulation. Conducted in vitro fluorescence/MRI imaging and cell viability assays, and in vivo nanoprobe accumulation evaluation in brain tumors.ResultsANG-BSA/BCNU/ICG MNPs exhibited superior targeting and cytotoxicity against GBM cells in vitro. In vivo, enhanced brain tumor accumulation during imaging was observed.ConclusionThis targeted imaging and drug delivery system holds promise for efficient GBM therapy and intraoperative localization, addressing Blood-brain barrier (BBB) limitations with precise drug delivery and imaging capabilities.
研究领域:
纳米技术
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神经肿瘤学
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药物递送系统
·
医学成像
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DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
·
2013
·Vol. 24
·期 9
·2093-2100
·
IF 2.141
Guo Rui,Chen Lili,Cai Shushan,Liu Zonghua,Zhu Yi,Xue Wei,Zhang Yuanming
Polysaccharides based polyelectrolyte complex nanoparticles (PCNs) intended for use in the delivery of macromolecules were prepared by the self-assembly of deoxycholic acid hydrophobically modified chitosan (CS-DCA) core and then coated with sodium alginate (ALG) shell. The CS-DCA capable of forming nano-sized self-aggregates in medium was prepared by the grafting of DCA to CS. In order to increase the stability of nanoparticles and prevent burst release of drug in bloodstream, polyanionic ALG was coated on the surface of positively charged CS-DCA nanoparticles to form PCNs. Dynamic light scattering results revealed that the mean diameter of the PCNs was about 330 nm, larger than that of uncoated nanoparticles (~150 nm). The zeta potential was big enough to keep the stability of PCNs (−28 mV); no size change was found even upon 1 month storage. Bovine serum albumin could be easily incorporated into the PCNs with encapsulation efficiency (>44 %) and keep a sustained manner without burst release when exposed to PBS (pH 7.4) at 37 °C. These results suggested that PCNs may be a promising drug carrier for a prolonged and sustained delivery in the bloodstream.
研究领域:
生物材料学
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药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials
·
2020
·Vol. 13
·期 16
·3478
·
IF 3.057
Hongpu Huang,Yefeng Feng,Jia Qu
A series of tem-responsive and protein-resistance property silica-di-block polymers SiO2-g-PMMA-b-P(PEGMA) hybrids are synthesized with methyl methacrylate (MMA) and poly (ethylene glycol) methyl ether methacrylate (PEGMA) by the surface-initiated atom transfer radical polymerization (SI-ATRP). The morphology in tetrahydrofuran (THF) solution, lower critical solution temperature (LCST), surface morphology, bovine serum albumin (BSA)-resistance property, and thermal stability of nanoparticles were analyzed. The results of1H-NMR, GPC, and TEM prove that the silica-di-block hybrids have been obtained. The silica-di-block hybrids shows the LCST (52–64 °C) in aqueous solution. The hybrid films casted by THF present distributed uniform granular bulges and the film surface is relatively smooth (Ra = 15.4 nm ~ 10.5 nm). The results of QCM-D showed that only a small amount of BSA protein(△f= 18.6 ~ 11.8 Hz) was adsorbed on the surface of the films. The result of XPS also demonstrated that only a small amount of BSA protein was absorbed onto the surface of the film (N% = 1.86). The TGA analyses indicate that the thermal decomposition temperature of hybrids is 288 °C. Thus, it is suggested that the hybrids are served as a suitable coating with BSA resistance property and thermal stability.Keywords:silica-di-block hybrids;LCST;surface properties;BSA resistance;coating
研究领域:
材料科学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Ting Qi,Dan Yang,Xianfu Chen,Wei Ke,Minghui Qiu,Yiqun Fan
Membranes are widely used for protein separation and purification in biotechnology and food processing. However, fouling remains challenges in membrane filtration processes and limits further industrial application. In this study, a facile method for constructing the antifouling alumina membrane via sulfonation modification was comprehensively investigated. FTIR, XPS, FESEM, EDS and zeta potential were used to characterize the sulfonated alumina membrane (Al 2 O 3 -SO 3 H membrane) and confirm that organosilanes grafted on the membrane surface and −SH groups were successfully oxidized to −SO 3 H. The Al 2 O 3 -SO 3 H membrane showed a strong negative charge in the neutral solution. In the antifouling test, the Al 2 O 3 -SO 3 H membrane exhibited a high permeate flux during the filtration of bovine serum protein (BSA) solution, and its stable permeate flux was approximately-eight times that of the original Al 2 O 3 membrane. This was attributed to the sulfonation of Al 2 O 3 membrane and enhanced electrostatic repulsion. The Al 2 O 3 -SO 3 H membrane also exhibited good antifouling performance in the filtration of bacteria-containing BSA solution. This was attributed to the electrostatic repulsion of the Al 2 O 3 -SO 3 H membrane against BSA molecules, which reduced the adsorption of BSA on the membrane surface and pore walls. Finally, the structural stability and repeatability of the Al 2 O 3 -SO 3 H membrane were investigated. The sulfonated alumina membranes exhibit excellent antifouling performance and are promising for the separation and purification of BSA-containing systems.
研究领域:
生物技术
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食品加工
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 33
·27485-27493
·
IF 3.289
Yunze Wang,Haibo Lin,Zhu Xiong,Ziyang Wu,Xuemin Yu,Yi Wang,Fu Liu
An interesting PVDF membrane with unusual thermoresponsive behavior was prepared by the incorporation of P(OEGMA-co-VTMOS). The P(OEGMA-co-VTMOS) copolymer was first in situ synthesized in a PVDF/triethyl phosphate (TEP) casting solution. A P(OEGMA-co-VTMOS) network was assembled in the PVDF membrane through hydrolysis and condensation during phase inversion. PVDF/P(OEGMA-co-VTMOS) in organic solvent demonstrated a typical LCST around 35 °C, reflecting the reversible transition of the coil-to-globule conformation. Microphase separation was responsible for the appearance of turbidity of the casting solution. FTIR, XPS, TGA and SEM confirmed the surface enrichment of the copolymer, especially in the membrane bottom. The hydrophilicity and protein anti adsorption were improved despite the temperature variation. In particular, AFM in aqueous media was conducted to determine the reversible morphology and water channel variation under heating and cooling. Different from common thermoresponsive membranes, the so-modified PVDF membranes exhibit a reversible abnormal change of pure water flux and BSA rejection with temperature. The intriguing thermoresponsive behavior and mechanism of the modified PVDF membrane was thoroughly investigated from the aspects of PVDF/TEP casting solution, membrane morphology in aqueous media and water/BSA filtration performance.
研究领域:
材料科学
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膜技术
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高分子化学
✓ 已验证
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2021
·
IF 8.128
Minjia Meng,Binrong Li,Yu Zhu,Yongsheng Yan,Yonghai Feng
Photocatalytic materials can be used as self-cleaning functional materials to alleviate the irreversible fouling of ultrafiltration membranes. In this work, the small size g-C 3 N 4 /Bi 2 MoO 6 (SCB) blended polysulfone (PSF) ultrafiltration membranes was fabricated by hydrothermal and phase inversion methods. As a functional filler of ultrafiltration membranes, the small size g-C 3 N 4 nanosheet decorated on the surface of Bi 2 MoO 6 can enhance the photocatalytic performance for bovine serum albumin (BSA) degradation, and remove irreversible fouling under visible light irradiation . In addition, the introduction of SCB microspheres into PSF matrix obviously increased the porosity of ultrafiltration membranes. Therefore, the SCB-PSF ultrafiltration membranes displayed excellent antifouling performance (flux recovery ratio is 82.53%) and BSA rejection rates (94.77%). SCB-PSF also had high photocatalytic self-cleaning activity, indicating excellent application prospects in organic wastewater treatment .
研究领域:
环境科学
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材料科学
✓ 已验证
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Yifei Zhu,Lili Yao,Zongguang Liu,Wenjian Weng,Kui Cheng
A facile strategy is needed for accurate time–space supply of suitable growth factors or drugs. Polypyrrole (PPy) was able to carry almost all kinds of negatively charged biomolecules through anodizing method, which made it an appropriate way for codeposition of multiple molecules. The difference in the conjugation between different molecules and PPy makes it possible for selective release when the redox state of PPy changes. In this work, bovine serum albumin (BSA) and heparin (Hep) were chosen to be the model molecules in view of their differences in the level of electronegativity and molecular weight. Double-layer deposition method was used to improve the biocompatibility of PPy/BSA/Hep film. It was found the content of BSA and Hep in the film can be controlled by regulating deposition current and time. BSA release was facilitated under positive voltage and then promote the proliferation of preosteoblasts, while Hep release was promoted under negative voltage and enhance cell differentiation. Our work provides a dual-molecule model in PPy for selective release and further explores the mechanism of release selectivity, this discovery has potential applications in tissue engineering and regenerative medicine.
研究领域:
生物材料学
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组织工程学
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再生医学
✓ 已验证
DOI
PubMed
高影响因子
RSC Advances
·
2022
·Vol. 12
·期 20
·12600-12606
·
IF 4.036
Shuxin Zhang,Hai Fan,Chunrong Yi,Ying Li,Kunmei Yang,Shenglong Liu,Ziqiang Cheng,Jianchao Sun
Zinc finger antiviral proteins (ZAP) can significantly inhibit the replication of avian leukosis virus subgroup J (ALV-J), but the traditional method of ZAP administration is by injection, which can easily cause stress effects in chickens. In this work, we established a sodium alginate/atractylodis macrocephalae system for the encapsulation of CCCH-type zinc finger antiviral protein (CCCH-ZAP). Because of the high cost of ZAP, we first chose bovine serum albumin (BSA) as a model protein to investigate the encapsulation performance. The SEM images clearly confirmed that BSA and the sodium alginate/atractylodis macrocephalae system can assemble easily to form relatively stable nanostructures, and the encapsulation amount of BSA can reach 68%. Subsequently, the encapsulation of ZAP was studied. The SEM and the encapsulation experiments confirmed that ZAP can also be assembly encapsulated in the sodium alginate/atractylodis macrocephalae system with the encapsulation amount of 80%. Release studies showed that the SA/AM-ZAP nanocomposite was able to achieve a release rate of 32% of ZAP. This work successfully confirms the assembly encapsulation of ZAP, which will be beneficial for the usage of ZAP-based animal drugs.
研究领域:
兽医学
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药学
·
纳米技术
✓ 已验证
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
·
IF 5.4
Yuansheng Lin,Zhilong Guo,Suling Dong,Yixin Li,Bohong Kan,Kongyin Zhao,Sidi Li,Zhengchun Yang
Hydrogels showed superiorities in molecularly imprinting sensors for detecting biomolecules owing to their stimulus-responsive nature. However, the excessive swelling of hydrogel during the elution process damages the imprinting cavities, leading to a reduction in sensitivity. In this study, a molecularly imprinted electrochemical sensor was designed for rapid protein recognition. The sensor utilized a composite hydrogel based on hybrid cross-linked calcium alginate/calcium silicate (CaAlg/CaSiO 3 ) to mitigate the excessive swelling of the CaAlg hydrogel. The preparation involved coating a bare carbon electrode with a sodium silicate-sodium alginate (Na 2 SiO 3 -NaAlg) mixed solution, cross-linking with CaCl 2 solution, and eluting bovine serum albumin (BSA) with Tris-HCl solution. The incorporation of CaSiO 3 nanoparticles stabilized the organic-inorganic hybrid structure, enhancing the hydrogel's resistance to swelling and maintaining cavity integrity. The molecularly imprinted polymer (MIP) sensor demonstrated high selectivity for BSA, excellent repeatability, long-term stability, and reproducibility, showing the potential for improved sensitivity in biomolecule detection.
研究领域:
生物传感技术
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材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF PHARMACY AND PHARMACOLOGY
·
2024
·
IF 2.8
Tang Hongping,Qiu Xinyu,Chen Yue,Yan Li,Zhao Jie,Cao Bing tao,He Yujing,Hao Jifu
ObjectivesOrientation to specific cells is an important topic in active targeting strategy for nanoparticle-based drug delivery systems. While these administered nanoparticles will be sequestrated within the liver, their cellular distribution behaviors in the liver are not clear. The aim of this study was to fabricate glycyrrhizic acid (GL) modified BSA nanoparticles and evaluate their hepatic cellular distribution.MethodsGL-modified BSA (GL-BSA) was tailored according to the periodate oxidation method, then GL-BSA nanoparticles loaded with paclitaxel (PTX@GL-BSA NPs) were prepared through self-assembly approach. In vitro cellular uptake was assessed by FITC-labeled BSA nanoparticles and immunofluorescent analysis was performed to track their relative distribution in the liver.Key findingsThe fabricated PTX@GL-BSA NPs were spherical structure with the particle size of 179 nm and a negative potential (−17.3 mV). Flow cytometry (FCM) studies exhibited that the accumulation of GL-BSA nanoparticles was 5.3-fold compared with BSA nanoparticles in HepG2 cells. The Nanoparticles were preferentially accumulated in the sinusoidal endothelial cells rather than the Kupffer cells.ConclusionsThis study provides useful information to understand the distribution of hepatic targeting nanoparticles when using GL-modified BSA nanoparticles, which helps to further use for effective treatment of liver disease.
研究领域:
药剂学
·
肝脏病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Research and Technology-JMR&T
·
2026
·
IF 7.3
Lei Wang,Zekun Liu,Han Zhang,Pengfei Xu,Zhihao Liu,Bo Wang,Fei Ding,Jiadai Xue,Langping Wang,Yanji yang,Yong Chen,Yupeng Xu,Dianlong Wang,Bo Wang
Achieving near-zero internal stress without sacrificing strength remains a key challenge in Ni-based electroforming. Here, a Co/BSA co-regulation strategy is developed using benzenesulfonamide (BSA) as a dual-functional additive. Theoretical calculations and electrochemical measurements show that the benzene ring promotes interfacial adsorption, while activation of the sulfonamide group facilitates S incorporation, shifting the internal stress from tensile to compressive. Dynamic BSA adsorption suppresses lateral and through-thickness grain growth, promotes nucleation, and drives the transition from columnar grains to refined equiaxed grains. Meanwhile, facet-dependent BSA adsorption drives a concentration-dependent texture transition by shifting the deposition mode from grain-growth-dominated at low BSA concentrations to nucleation-dominated at high concentrations, favoring (200)- and (111)-oriented grains, respectively. The resulting texture evolution and S segregation cause a mismatch between the low-stress and high-hardness windows. To overcome this trade-off, Co is introduced for synergistic regulation. Co incorporation provides tensile-stress compensation and solid-solution strengthening, shifting the near-zero-stress state toward the high-strength region. Consequently, Co/BSA co-regulation enables the fabrication of near-zero-stress Ni-Co deposits with a hardness of 549.3 HV 0.1 and a tensile strength of 1598.2 MPa. This work provides a strategy for designing low-stress and high-strength electroformed Ni-based materials.
✓ 已验证
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2017
·
IF 4.811
Yang Liu,Haitao Huang,Pengfei Huo,Jiyou Gu
This study focused on the preparation of a new kind of membrane material, zwitterionic cellulose acetate (ZCA), via a three-step procedure consist of oxidization, Schiff base and quaternary amination reaction, and the fabrication of antifouling ZCA ultrafiltration membrane by the non-solvent-induced phase separation method (NIPS). The morphologies, surface chemical structures and compositions of the obtained CA and ZCA membranes were thoroughly characterized by field emission scanning electron microscopy (FE-SEM) with energy dispersive X-ray (EDX) spectroscopy, Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS), respectively. Meanwhile, the thermal stability, porosity and average pore size of two investigated membranes were also studied. As a result, the ZCA membrane displayed significantly improved hydrophilicity and water permeability compared with those of the reference CA membrane, despite a slight decrease in the protein rejection ratio. According to the cycle ultrafiltration performance of bovine serum albumin (BSA) solution and protein adsorption experiment, ZCA membrane exhibited better flux recovery property and fouling resistant ability, especially irreversible fouling resistant ability, suggesting superior antifouling performance. This new approach gives polymer-based membrane a long time life and excellent ultrafiltration performance, and seems promising for potential applications in the protein separation.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
DOI
高影响因子
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