ENVIRONMENTAL GEOCHEMISTRY AND HEALTH
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2025
·Vol. 47
·期 12
·568
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IF 3.8
Xie Hongguan,Gong Lian,Lei Shiwei,Li Shida,Feng Yuxin,Liao Lin,Wang Yue,Wang Kongxi
Polycyclic aromatic hydrocarbons (PAHs) pose a significant threat to the ecological environment, and plant–microbe remediation represents a promising strategy for the treatment of contaminated soils. This study aimed to evaluate the effects of foliar application of selenite or selenium nanoparticles (SeNPs) on the remediation efficiency of pyrene by Alfalfa ( Medicago sativa L.) and Bacillus subtilis PSL-1. A pot experiment was conducted with foliar selenium treatments at different concentrations (5–15 mg kg −1 ), and the effects were comparatively analyzed in terms of plant physiological parameters (chlorophyll content, biomass, and antioxidant enzyme activity), soil enzyme activities (dehydrogenase and catalase), soil pyrene speciation, and microbial community structure. The results showed that low-concentration foliar selenium application (5 mg L −1 ) enhanced antioxidant defense, alleviated oxidative stress, and promoted chlorophyll synthesis and growth of Alfalfa, thereby stimulating soil enzyme activity and enriching pyrene-degrading microorganisms. Consequently, the pyrene degradation rate increased from 62.07% to 67.83–71.91%. In contrast, high selenite concentration (15 mg L −1 ) induced phytotoxicity and weakened the stimulation of soil enzyme and microbial activities. Compared with selenite, SeNPs consistently enhanced the remediation efficiency across all tested concentrations, demonstrating a broader safe concentration range. Overall, foliar selenium application effectively promoted plant–microbe remediation of pyrene-contaminated soil, and SeNPs exhibiting a superior promoting effect and higher application potential than selenite.
研究领域:
环境科学
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植物生理学
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土壤微生物学
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纳米技术
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植物修复
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JOURNAL OF HAZARDOUS MATERIALS
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2023
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IF 13.6
Li-Hui Liu,Jia-Yan Zhang,Guang-Xuan Tang,Yu-Hong Huang,Xiang-Qing Xie,Jun Geng,Hui-Xiong Lü,Hui Li,Yan-Wen Li,Ce-Hui Mo,Hai-Ming Zhao,Quan-Ying Cai
Endophytic bacteria can degrade toxic phthalate (PAEs). Nevertheless, the colonization and function of endophytic PAE-degrader in soil-crop system and their association mechanism with indigenous bacteria in PAE removal remain unknown. Here, endophytic PAE-degrader Bacillus subtilis N-1 was marked with green fluorescent protein gene. Inoculated strain N-1- gfp could well colonize in soil and rice plant exposed to di-n-butyl phthalate (DBP) as directly confirmed by confocal laser scanning microscopy and realtime PCR. Illumina high-throughput sequencing demonstrated that inoculated N-1- gfp shifted indigenous bacterial community in rhizosphere and endosphere of rice plants with significant increasing relative abundance of its affiliating genus Bacillus than non-inoculation. Strain N-1- gfp exhibited efficient DBP degradation with 99.7% removal in culture solutions, and significantly promoted DBP removal in soil-plant system. Strain N-1- gfp colonization help plant enrich specific functional bacteria (e.g., pollutant-degrading bacteria) with significant higher relative abundances and stimulated bacterial activities (e.g., pollutant degradation) compared with non-inoculation. Furthermore, strain N-1- gfp displayed strong interaction with indigenous bacteria for accelerating DBP degradation in soil, decreasing DBP accumulation in plants and promoting plant growth. This is the first report on well colonization of endophytic DBP-degrader Bacillus subtilis in soil-plant system and its bioaugmentation with indigenous bacteria for promoting DBP removal.
研究领域:
环境微生物学
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土壤学
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植物-微生物互作
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PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2026
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IF 4.2
Yang Yang,Yu-Xin Zhou,Le-Yao Jia,Xin-Yang Song,Ya-Jie Lai,Si-Yuan Liu,Su-Yan Wang,Pedro Laborda,Xin-Chi Shi
BACKGROUND The spoVF operon, encoding the dipicolinic acid (DPA) synthase, is critical for sporulation in Bacillus subtilis . A recent study suggested that spoVF expression in vegetative cells promotes biofilm formation, yet the detailed regulatory pathways and the underlying metabolic connections remain to be fully elucidated. RESULTS In this study, deletion of spoVF subunits spoVFA and spoVFB severely reduced DPA synthesis, downregulated mreB gene expression, and impaired biofilm formation, with mutant ΔA showing stronger effects than mutant ΔB. Although wild type B. subtilis 168 cells exhibited rod shape, double mutant ΔAΔB cells were spherical. In the MT strain, engineered for enhanced spoVF expression during vegetative growth, the upregulation of key TCA cycle genes (e.g., citC ) and glutamate synthesis genes (e.g., gltA ) was observed. This metabolic reprogramming was accompanied by the promotion of Spo0A phosphorylation. The activated Spo0A~P then inhibited the SinR and AbrB repressors, which led to the activation of the eps and tasA biofilm operons. The MT strain showed superior stress tolerance to high temperature (42 °C), salt, acidic and alkaline pH, and UV-A and UV-B radiation. Additionally, the MT strain exhibited higher nutrient competition ability, and in vitro and in vivo antifungal activities against the sweet potato ( Ipomoea batatas L. Lam) storage root pathogen Ceratocystis fimbriata compared to mutant ΔA, ΔB, and ΔAΔB strains. CONCLUSIONS This study elucidates how the spoVF operon links the central metabolic pathways with B. subtilis cell morphology and biofilm formation, offering a novel target for engineering advanced Bacillus -based biocontrol agents. © 2026 Society of Chemical Industry.
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Food Bioscience
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2025
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IF 5.9
Lan Zhang,Yiming Zhou,Minglong Wang,Zhaofeng Li,Caiming Li
Diabetes mellitus is a chronic metabolic disorder. Although natural components such as polyphenols and polysaccharides have demonstrated anti-diabetic potential, the in vivo synergistic effects of their combinations remain poorly understood. This study explored the hypoglycemic effects and molecular mechanisms of Bacillus subtilis exopolysaccharides (BS-EPS) and their combination with epigallocatechin gallate (EGCG) in type 2 diabetes mellitus (T2DM) mice. Monotherapy with BS-EPS (200 mg/kg·bw/day) or EGCG (100 mg/kg·bw/day) significantly reduced fasting blood glucose levels by 15.3 % and 15.7 %, respectively, while combination therapy showed a superior reduction of 25.4 %. Moreover, the combined treatment significantly improved insulin sensitivity and alleviated hepatic injury. The combined treatment modulated the gut microbiota composition by enriching beneficial bacteria (including Faecalibaculum , Akkermansia , and Parabacteroides ) while enhancing short-chain fatty acid production, particularly acetate. Metabolomic analysis revealed that the combined treatment altered bile acids and glycerophospholipids profiles, while modulating key metabolic pathways including choline metabolism, glycerophospholipid metabolism, and unsaturated fatty acid biosynthesis. These findings suggest that the combined intervention of BS-EPS and EGCG may synergistically ameliorate hyperglycemia through gut microbiota-metabolite-pathway interactions, supporting their potential as a dietary intervention for T2DM.
研究领域:
营养生物化学
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微生物组研究
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代谢组学
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糖尿病研究
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功能食品
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高影响因子
Journal of Environmental Chemical Engineering
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2025
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IF 7.2
Qiwen An,Jie Liu,Yitong Liu,Fuxuan Bi,Zonghao Dong,Xu Liu,Zhao Jiang,Libo Fu,Hongli Fan,Jianhua Qu,Ying Zhang
Herein, an innovative coupling remediation system integrating a functionalized bacterial agent (BBC-BS) derived from bone char (BC) and Bacillus subtilis (BS) combined with smooth vetch ( Vicia villosa var.) (SV) was developed and applied to actual Cd-contaminated farmland soil. Results revealed that mechanical ball milling significantly enhanced the S BET of BBC-BS, refined its particle size, and modified its crystalline structure, enabling effective BS immobilization while providing a stable microenvironment. This optimization elevated the phosphate solubilization capacity, representing a 26.9% increase compared to free BS. The coupled BBC-BS+SV system exhibited dual synergistic effects during plant growth: (1) BBC-BS promoted SV growth, while (2) markedly suppressing Cd accumulation in SV. Furthermore, after 28 d of SV pressed into soil, the DTPA-extractable Cd content treated by BBC-BS+SV decreased by 53.1% compared to the control (CK), surpassing the combined remediation efficiencies of individual BBC-BS application and SV incorporation alone (37.3%). The application of BBC-BS not only stimulated SV biomass accumulation but also accelerated SV residue decomposition via functional microbiota activation, generating more low-molecular-weight organic acids (LMWOAs) and humic acid (HA). Reciprocally, these products enhanced active sites on the BBC-BS surface, promoted Ca and P release from BBC-BS, and further strengthened Cd passivation. The coupled system ensured compliance with Cd residue standards in pepper while significantly improving fruit nutritional quality, achieving simultaneous soil remediation and safe crop production. This strategy provided a practical solution for Cd-contaminated farmland rehabilitation and sustainable enhancement of subsequent crop yield and quality.
研究领域:
环境科学
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土壤修复
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农业生态学
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微生物生物技术
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植物-微生物互作
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
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IF 6.2
Xinrui Tang,Abdullah Arsalan,Guoyan Zhang,Junhua Yun,Cunsheng Zhang,Xianghui Qi
D-Allose, a rare sugar, has gained significant attention not only as a low-calorie sweetener but also for its anticancer, antitumor, anti-inflammatory, antioxidant, and other pharmaceutical properties. Despite its potential, achieving high-level biosynthesis of D-allose remains challenging due to inefficient biocatalysts, low conversion rates, and the high cost of substrates. Here, we explored the food-grade coexpression of Blautia produca D-allulose 3-epimerase (Bp-DAE) and Bacillus subtilis L-rhamnose isomerase (BsL-RI) within a single cell using B. subtilis WB800N as the host. Using this system, D-allose was synthesized via a simple, cost-effective, one-pot enzymatic process, employing whole cells as catalysts and D-fructose as the substrate. The system exhibited optimal activity at 65 °C, pH 8.5, with 1 mM Mn2+ and 20 g/L of whole-cell dry weight. Initial production reached 12.5 g/L D-allose with a 12.5% yield from 100 g/L D-fructose. Optimization of dual promoter combinations enhanced production, achieving 15.0, 29.1, and 43.2 g/L D-allose from 100, 200, and 300 g/L D-fructose, with yields of 15.00, 14.55, and 14.40%, respectively. This D-allose production biocatalyst offers a scalable and economically viable platform for the industrial production of rare sugar.
研究领域:
生物化学
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食品科学
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工业微生物学
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PubMed
高影响因子
MICROBIOLOGY
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2017
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IF 0.856
Faheem Uddin Rajer,Huijun Wu,Yongli Xie,Shanshan Xie,Waseem Raza,Hafiz Abdul Samad Tahir,Xuewen Gao
Rhizobacterial volatile organic compounds (VOCs) play an important role in the suppression of soil-borne phytopathogens. In this study, the VOCs produced by a soil-isolate,Bacillus subtilisFA26, were evaluatedin vitrofor their antibacterial activity againstClavibacter michiganensisssp.sepedonicus(Cms), the causal agent of bacterial ring rot of potato. The VOCs emitted by FA26 inhibited the growth of Cms significantly compared with the control. Scanning and transmission electron microscopy analyses revealed distorted colony morphology and a wide range of abnormalities in Cms cells exposed to the VOCs of FA26. Varying the inoculation strategy and inoculum size showed that the production and activity of the antibacterial VOCs of FA26 were dependent on the culture conditions. Headspace solid-phase microextraction/gas chromatography–mass spectrometry analyses revealed that FA26 produced 11 VOCs. Four VOCs (benzaldehyde, nonanal, benzothiazole and acetophenone) were associated with the antibacterial activity against Cms. The results suggested that the VOCs produced by FA26 could control the causal agent of bacterial ring rot of potato. This information will increase our understanding of the microbial interactions mediated by VOCs in nature and aid the development of safer strategies for controlling plant disease.
研究领域:
植物病理学
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微生物学
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农业科学
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DOI
PubMed
高影响因子
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