TALANTA
·
2018
·
IF 4.244
Xiu-Fang Yan,Zeng-Ping Chen,Ying Huang,Chao Kang,Ru-Qin Yu
Probe encapsulated by biologically localized embedding (PEBBLE) has emerged as a new type of sensing technique for complex systems. Generalized ratiometric PEBBLE nanosensors prepared by encapsulating an intensity-based probe and an inert reference dye inside the pores of stable matrix possess advantages of easy synthesis, immunity to interference, lower toxicity, and robustness to variations in probe loading. However, the selection of appropriate reference dyes used in generalized ratiometric PEBBLE nanosensors is a rather difficult task since they should satisfy some stringent requirements. In this contribution, the feasibility of using carbon dots (C-dots) as generic inert references in synthesizing PEBBLE nanosensors was first investigated in detail. And a dual-wavelength monitoring strategy and the quantitative fluorescence model for generalized ratiometric probes (QFM GRP ) were adopted to solve the problems brought by the use of carbon dots as inert references. C-dots doped PEBBLE nanosensors (C-PEBBLE nanosensors) for the quantification of NO 2 − and free Ca 2+ were synthesized by encapsulating C-dots and intensity based fluorescence probes (i.e., acriflavine for NO 2 − , and Rhod-2 for Ca 2+ , respectively) inside the pores of stable matrix. Experimental results showed that the combination of C-PEBBLEs, the QFM GRP model and the dual-wavelength monitoring strategy achieved accurate quantification of NO 2 − and the free Ca 2+ in real-world samples. Their quantitative results were in good consistence with those determined by HPLC and atomic absorption spectrophotometer, respectively. The strategies proposed in this contribution have generic applicability in the synthesis of PEBBLE nanosensors and their quantitative applications.
研究领域:
生物化学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
·
2023
·
IF 6.1
Fuqing Bai,Guijie Chen,Huiliang Niu,Hongliang Zhu,Ying Huang,Mingming Zhao,Ruyan Hou,Chuanyi Peng,Hongfang Li,Xiaochun Wan,Huimei Cai
The effects of different brewing water samples, including natural drinking water (NDW), pure water (PW), mineral water (MW), distilled water (DW), and tap water (TW) on flavor and quality of green tea infusion were investigated. The results showed the dissolution rate of mineral substances varied greatly depend on the type of water used. Notably, the tea infusion brewed with MW showed the highest taste response and darker but higher brightness in color. Furthermore, the content of volatile compounds was highest in tea infusion brewed with NDW and lowest in tea infusion brewed with MW. The mineral substances content and pH were the main factors affecting volatile compounds in green tea infusion. Thereinto, Ca 2+ and Fe 3+ remarkably affected the content of alcohols and aldehydes in volatile compounds. These results suggested that water with a neutral pH value and lower mineral substance content is more conducive for brewing green tea.
研究领域:
食品科学
·
化学
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产品关联
DOI
PubMed
高影响因子
Materials Today Communications
·
2025
·
IF 4.5
Suwanan Thammarong,Xiaogang Yu,Peinan Yin,Renpeng Yang,Chengwei Wu,Wei Zhang
Magnetic induction hyperthermia has great potential in cancer treatment. However, the low heating efficiency of the magnetic nanoparticles (MNPs) with self-regulated temperature capability limits its clinical application. Here, the self-regulated temperature MNPs of Ca-doped Co-Zn ferrite MNPs (Ca x Co 0.5-x Zn 0.5 Fe 2 O 4 , 0 ≤ x ≤ 0.20), with high heating efficiency, were successfully synthesized by co-precipitation method followed by heat treatment. Noticeably, the x = 0.1 suspension sample can self-stabilize the temperature for high-temperature hyperthermia, whereas x = 0.2 suspension sample can self-stabilize the temperature suitable for conventional hyperthermia under an alternating magnetic field of 32 kA/m and 100 kHz. In vitro cell experiments indicate that the MNPs have good biocompatibility. The results demonstrate that optimized saturation magnetization ( σ S ) and tunable Curie temperature (Tc) enhance both the heating efficiency and thermal safety of the nanoparticles during magnetic induction hyperthermia treatment.
研究领域:
生物医学工程
·
材料科学
·
肿瘤治疗
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Jie Wu,Jinhua Li,Mingming Cheng,Li Li,Ruhong Yan,Juan Yue
Calcium ions (Ca 2+ ) are key players in intracellular signaling as second messengers and play a pivotal role in various physiological processes. In this study, near-infrared water-soluble AgInS 2 quantum dots (AIS QDs) for Ca 2+ detection were synthesized by a one-step hydrothermal method. The fluorescence quantum yield (PL QY) of the quantum dots was as high as 23.99 %. With low cytotoxicity and good fluorescence properties, as well as short reaction time, the ternary AIS QDs have excellent synthesis efficiency and quantum yield, which are advantageous for Ca 2+ detection and bioimaging applications. The fluorescence quenching of the quantum dots showed a clear linear relationship with calcium ion concentration in the range of 0–250 μM (detection limit: 0.65 μM). Confocal imaging experiments demonstrate the excellent biofluorescence imaging capability of AIS QDs. By tuning the Ag/In molar ratio, AIS QDs can achieve fluorescence emission in the near-infrared wavelength band (620–700 nm), and the near-infrared fluorescence imaging has deeper tissue penetration, less tissue absorption and photodamage, and lower interference of spontaneous fluorescence, which further expands the potential of QDs for bioimaging applications.
研究领域:
生物化学
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医学影像
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材料科学
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产品关联
DOI
PubMed
高影响因子
PLoS One
·
2013
·Vol. 8
·期 10
·e76264
·
IF 3.73
Liangbin Hu,Dede Wang,Li Liu,Jian Chen,Yanfeng Xue,Zhiqi Shi
As a destructive fungus-like plant pathogen, the oomycete Phytophthora capsici is unable to synthesize its own ergosterol as the potential target of fungicide cinnamaldehyde (CA). In this study, CA exerted efficient inhibitory effects on both mycelial growth (EC50=0.75 mM) and zoospore germination (MIC=0.4 mM) of P . capsici . CA-induced immediate Ca 2+ efflux from zoospores could be confirmed by the rapid decrease in intracellular Ca 2+ content determined by using Fluo-3 AM and the increase in extracellular Ca 2+ concentration determined by using ICP-AES (inductively coupled plasma atomic emission spectrometry). Blocking Ca 2+ influx with ruthenium red and verapamil led to a higher level of CA-induced Ca 2+ efflux, suggesting the simultaneous occurrence of Ca 2+ influx along with the Ca 2+ efflux under CA exposure. Further results showed that EGTA-induced decrease in intracellular Ca 2+ gave rise to the impaired vitality of P . capsici while the addition of exogenous Ca 2+ could suppress the growth inhibitory effect of CA. These results suggested that Ca 2+ efflux played an important role in CA-induced growth inhibition of P . capsici . The application of 3-phenyl-1-propanal, a CA analog without α,β- unsaturated bond, resulted in a marked Ca 2+ influx in zoospores but did not show any growth inhibitory effects. In addition, exogenous cysteine, an antagonist against the Michael addition (the nucleophilic addition of a carbanion or another nucleophile) between CA and its targets, could attenuate CA-induced growth inhibition of P . capsici by suppressing Ca 2+ efflux. Our results suggest that CA inhibits the growth of P . capsici by stimulating a transient Ca 2+ efflux via Michael addition, which provides important new insights into the antimicrobial action of CA.
研究领域:
植物病理学
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微生物学
·
农药科学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF RARE EARTHS
·
2021
·
IF 3.712
Zhao Cao,Zeyu Cheng,Jieliang Wang,Yongdan Cao
Bastnaesite is an important rare earth mineral and is usually beneficiated by flotation . Sodium silicate is commonly used to depress calcium-bearing gangue minerals, however it can also depress bastnaesite when Ca 2+ ions exist in the pulp. In this study, the effect of Ca 2+ ions and sodium silicate individually or in combination on bastnaesite flotation was studied through micro-flotation, zeta potential , fluorescence spectroscopy and X-ray photoelectron spectroscopy (XPS) measurements. Micro-flotation results show that the combination of Ca 2+ ions and sodium silicate depresses bastnaesite more severely due to their synergistic effect. Zeta potential results show that the combination renders the surface potential of bastnaesite negatively shifted more significantly. Fluorescence spectroscopy shows that the combination decreases the surface hydrophobicity of bastnaesite more severely. XPS shows that the combination increases the adsorption of sodium silicate on bastnaesite by forming hydrophilic Ca-SiO 3 precipitate, which causes more serious depression on bastnaesite flotation.
研究领域:
矿物学
·
化学工程
✓ 已验证
DOI
高影响因子
JOURNAL OF LUMINESCENCE
·
2023
·
IF 3.6
Licheng Yu,Youchao Wei,Yuanchao Lei,Yongsheng Liu,Maochun Hong
Inorganic cesium lead halide (CsPbX 3 , X = Cl, Br, or I) perovskite nanowires (NWs) are promising materials for investigating charge transfer and carrier dynamics at the nanoscale level. However, the synthesis of CsPbX 3 NWs with well-defined cubic phases and the desirable properties remains challenging. In this study, we successfully synthesized uniform-diameter cubic-phase CsPbBr 3 NWs with favorable properties via trace Ca 2+ doping. By combining experimental results with first-principles calculations, we confirmed that Ca 2+ doping not only induces lattice distortion in cubic-phase CsPbBr 3 nanocubes, triggering dipole moments that drive self-assembly into single-crystalline NWs via oriented attachment but also inhibits the formation of surface defects and significantly modulates the exciton relaxation kinetics by reducing nonradiative recombination, thus enhancing the photoluminescence efficiency and stability of perovskite NWs with high crystallinity . Furthermore, by exploiting the high plasticity of the CsPbX 3 lattice, we demonstrated the ability to tune the composition and emission color of the NWs through anion exchange reactions while preserving their morphology and crystal structure. These findings provide a stable platform for preparing highly efficient perovskite materials with satisfactory optoelectronic properties for use in prospective photovoltaic and optoelectronic devices.
研究领域:
材料科学
·
光学
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产品关联
DOI
高影响因子
JOURNAL OF POLYMERS AND THE ENVIRONMENT
·
2022
·Vol. 31
·期 4
·1628-1641
·
IF 4.705
Zhou Qichang,Zhang Siqi,Peng Yang,Fang Xiuqin,Zhao Xinyu,Yu Gaobo,Xie Yanli,Li Jiacheng,Feng Yuhong
Agrochemicals provide huge agronomic and economic benefits. However, excessive agrochemicals cause serious environmental pollution. The use of surfactants can effectively improve the utilization rate of agrochemicals in the soil, and an amphiphilic alginate derivative (Ugi-alg) was prepared in this work. This paper mainly discussed the affinity between Ugi-alg and soil colloids and the influence of Ugi-alg on the adsorption behavior of pesticides on colloidal particles. In the presence of 2.4 mM Ca2+, the affinity between soil colloids and Ugi-alg (30 mg/L) was enhanced (~ − 12.33 mV) compared with pure soil colloids (~ − 30 mV). The strong affinity between Ugi-alg and the colloids reduced the migration of acetamiprid. Transmission electron microscopy showed that soil colloids were wrapped by the network structure of Ugi-alg, indicating that Ugi-alg easily adhered to the surface of soil colloids. Studies of the adsorption showed the adsorption capacity of acetamiprid on soil colloids was 556 mg/g in the presence of Ugi-alg (30 mg/L) and Ca2+ (2.4 mM). And the adsorption behavior of acetamiprid in soil colloids was also not greatly affected by changes in pH and temperature. Ugi-alg is a degradable polysaccharide-based surfactant which provides a new concept and a practical method for the remediation of soil pollutants.Graphical abstract
研究领域:
环境科学
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农业化学
·
土壤学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Lihui Tan,Xiang Zhou,Keke Wu,Deguang Yang,Yanpeng Jiao,Changren Zhou
Massive blood loss and bacterial infection are major challenges for global public health. However, traditional wound dressings cannot fully meet the current clinical needs in controlling bleeding and avoiding infection. In this study, we prepared a chitin nanofiber suspension by green mechanical grinding and homogenizing, which could be used for developing a porous chitin nanofiber sponge by freeze drying. Then tannic acid/Ca II was anchored on the surface of chitin nanofiber sponge by layer-by-layer deposition based on the coordination complex of tannic acid and Ca 2+ . These porous chitin nanofiber sponges with meso-macroporous structure could accelerate platelet aggregation for hemostasis. Hemostasis tests also demonstrated that the Ca 2+ and TA in the TA/Ca II coating on chitin nanofiber sponge could accelerate platelet activity and shorten the hemostatic time both in vitro and in vivo . Besides, the TA in the TA/Ca II coating enhanced the antibacterial properties of the sponge, which depended the content of TA/Ca II . Based on these results, it could be inferred that the chitin nanofiber sponges anchored TA/Ca II coating had rapid hemostatic and antibacterial properties, which would have great potential for designing hemostatic product in clinical application.
研究领域:
材料科学
·
生物材料
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FEMS MICROBIOLOGY LETTERS
·
2014
·Vol. 357
·期 1
·99-103
·
IF 2.046
Wang Xian-Kun,Jin Jian-Ling
Streptomyces netropsis SD-07, the producer of novel polyene macrolide antifungal antibiotics, was isolated from soil. For the investigation of the functions of its biosynthesis genes and regulation mechanisms, a genetic operating system is necessary. In this study, we successfully transferred the plasmid DNA of pSET152 from the methylation deficient donor, Escherichia coli ET12567/pSET152/pUZ8002, to S. netropsis SD-07 by conjugation and evaluated the crucial factors influencing the conjugation frequency. Ca2+ ions in presence the conjugation media may increase the conjugation frequency by 1000–10 000 times than Ca2+ ions absence in the same conjugation media, and 10–100 time higher than Mg2+ ions. Similar results (increasing the conjugation frequency by 10–100 times when media containing 60 mM CaCl2) were also obtained from the conjugation between E. coli ET12567 and Streptomyces coelicolor, S. lavendulae, S. venezuelae, despite their conjugation media were different (MS, CM, GS). So, CaCl2 concentration is a crucial factor for increasing the conjugation frequency, and the suitable concentration may probably be 60 mM. In addition, synthetic medium containing a small amount of organic nitrogen source may benefit increasing the conjugation frequency. These findings could be valuable for the development of a practical method for achieving conjugation in other Streptomyces spp.
研究领域:
微生物学
·
遗传学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2021
·
IF 6.707
Zexiang He,Deshun Huang,Guozong Yue,Jing Zhu,Pengxiang Zhao
Three-dimensional (3D) porous MXene gel (MXene-Ca) was synthesized through a fast gelation of Ti 3 C 2 T x MXene nanosheets initiated by calcium ions. The MXene-Ca showed efficient phosphate adsorption ability through the strong chemical reaction between Ca 2+ and phosphate with a fast adsorption equilibrium (within 2 min). Furthermore, the recovered P/MXene-Ca gel after phosphate sorption could be used for adsorption of uranium in aqueous solution. Various analysis including SEM, TEM, XRD, XPS, FT-IR, BET surface area and zeta potential were carried out to fully characterize the materials. Increased specific surface area of the 3D porous structure and the formation of newly active adsorption sites of hydroxyapatite in P/MXene-Ca gel, are two main factors promoting the removal of U(VI). The maximum adsorption capacity was 823.6 mg/g at 298 K by Langmuir isotherm model. Kinetic studies disclosed that the of U(VI) followed the major chemical adsorption process. Overall results from batch experiments, leaching experiments, and characterizations demonstrated that the main mechanisms of the U(VI) removal by P/MXene-Ca including chemical adsorption on hydroxyapatite via dissolution precipitation and surface complexation with the oxygen-containing functional groups of MXene.
研究领域:
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
BIOMACROMOLECULES
·
2023
·
IF 6.2
Xiaopeng Fan,Renjian Xie,Wenjing Song,Kunfu Ouyang,Li Ren
Bionic mimics using natural cartilage matrix molecules can modulate the corresponding metabolic activity by improving the microenvironment of chondrocytes. A bionic brush polymer, HA/PX, has been found to reverse the loss of cartilage extracellular matrix (ECM) and has promising applications in the clinical treatment of osteoarthritis (OA). However, the unknown bioremediation mechanism of HA/PX severely hinders its clinical translation. In OA, the massive loss of the ECM may be attributed to a decrease in transient receptor potential vanilloid 4 (TRPV4) activity, which affects reactive oxygen species (ROS) clearance and [Ca2+]i signaling, initiating downstream catabolic pathways. In this study, we investigated the bioremediation mechanism of HA/PX in a model of interleukin 1β (IL-1β)-induced inflammation. Through TRPV4, HA/PX reduced ROS accumulation in chondrocytes and enhanced [Ca2+]i signaling, reflecting a short-term protection capacity for chondrocytes. In addition, HA/PX balanced the metabolic homeostasis of chondrocytes via TRPV4, including promoting the secretion of type II collagen (Col-II) and aggrecan, the major components of the ECM, and reducing the expression of matrix metal-degrading enzyme (MMP-13), exerting long-term protective effects on chondrocytes. Molecular dynamics (MD) simulations showed that HA/PX could act as a TRPV4 activator. Our results suggest that HA/PX can regulate chondrocyte homeostasis via ROS/Ca2+/TRPV4, thereby improving cartilage regeneration. Because the ECM is a prevalent feature of various cell types, HA/PX holds promising potential for improving regeneration and disease modification for not only cartilage-related healthcare but many other tissues and diseases.
研究领域:
生物材料
·
生物力学
·
组织工程
·
再生医学
·
骨科
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）