Innovative Food Science & Emerging Technologies
·
2024
·
IF 6.3
Li-Hao Wang,Wen-Hui Qu,Hui Jiang,Song-Gang Xia,Xue Feng,Chang-Hu Xue,Yun-Qi Wen
Gluten is widely used in food process, but rarely used in the fermentation of umami condiment. The extrusion treatment could modify the microstructure of gluten, which may further influence fermentation effectiveness. This study applied gluten and modified gluten obtained by extrusion as raw materials to koji and moromi fermentations. During the koji fermentation, water and Aspergillus oryzae applied to modified gluten penetrated into the inner layer of raw material more efficiently when compared with their application to gluten. After the fermentation, the amino acid nitrogen contents of prepared four group samples ranged from 0.80 g/100 mL to 1.05 g/100 mL, while the contents of glutamic acid, adenosine monophosphate (AMP), guanosine monophosphate (GMP), acetic acid and lactic acid etc. had significant increases, indicating the utilization of gluten in fermentation could produce large amounts of flavor compounds. Additionally, the modification of gluten accelerated the fermentation of A. oryzae and improved the utilization and efficiency of fermentation raw materials, while the soybean protein isolate could partially replace gluten to produce high amounts of flavor compounds during the fermentation. This study broadened the utilization scope of gluten in food industry and provided a reference for the fermentation of umami condiment.
研究领域:
食品科学
·
微生物学
✓ 已验证
DOI
高影响因子
JOURNAL OF ECONOMIC ENTOMOLOGY
·
2019
·Vol. 112
·期 5
·2142-2148
·
IF 1.779
Tian Jun-Ce,Chen Yang,Shelton Anthony M,Zheng Xu-Song,Xu Hong-Xing,Lu Zhong-Xian
Most adult parasitoids depend on carbohydrate-rich food as an energy source for longevity, fecundity, and mobility. Thus, providing sugars has been proposed as a technique to maximize the biological control efficacy of parasitoids. However, the sugars provided for parasitoids need to be carefully selected because herbivore hosts might also benefit. Here we explore the effects of 12 naturally occurring sugars on the longevity and fecundity of the rice pest, Chilo suppressalis, and the longevity of its parasitoid, Cotesia chilonis, as well as the effect of sugars on sugar consumption of C. chilonis. Results showed that none of the tested sugars significantly impacted the longevity of C. suppressalis, but fructose, glucose, maltose, and sucrose significantly increased its fecundity. The longevity of C. chilonis was significantly increased when fed fructose, glucose, sucrose, maltose, trehalose, and melezitose. Our data suggest that C. chilonis consumed larger quantities of glucose, fructose, and sucrose followed by maltose, melezitose, and trehalose and the longevity of C. chilonis was positively correlated to sugar consumption. Our data also suggest that the herbivore C. suppressalis and its parasitoid C. chilonis responded differently to trehalose and melezitose. Although additional studies are needed, our data suggest that these sugars could be considered as candidate components for sugar sprays to enhance the activity and efficacy of C. chilonis, but without benefiting its pest host.
研究领域:
昆虫学
·
生态学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 5
·2693
·
IF 4.9
Xiaofan Liu,Huihui Zhao,Jiatao Xie,Yanping Fu,Bo Li,Xiao Yu,Tao Chen,Yang Lin,Daohong Jiang,Jiasen Cheng
Phytopathogenic fungi normally secrete large amounts of CWDEs to enhance infection of plants. In this study, we identified and characterized a secreted glycosyl hydrolase 5 family member inSclerotinia sclerotiorum(SsGH5,Sclerotinia sclerotiorumGlycosyl Hydrolase 5).SsGH5was significantly upregulated during the early stages of infection. Knocking outSsGH5did not affect the growth and acid production ofS. sclerotiorumbut resulted in decreased glucan utilization and significantly reduced virulence. In addition,Arabidopsis thalianaexpressing SsGH5 became more susceptible to necrotrophic pathogens and basal immune responses were inhibited in these plants. Remarkably, the lost virulence of theΔSsGH5mutants was restored after inoculating ontoSsGH5transgenicArabidopsis. In summary, these results highlight thatS. sclerotiorumsuppresses the immune responses ofArabidopsisthrough secreting SsGH5, and thus exerts full virulence for successful infection.
研究领域:
植物病理学
·
分子生物学
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF EXPERIMENTAL BIOLOGY
·
2018
·Vol. 221
·期 5
·
IF 3.179
Zhang Junjun,Wang Zhengwei,Wen Ping,Qu Yufeng,Tan Ken,Nieh James C.
The nectar of the thunder god vine, Tripterygium hypoglaucum , contains a terpenoid, triptolide (TRP), that may be toxic to the sympatric Asian honey bee, Apis cerana , because honey produced from this nectar is toxic to bees. However, these bees will forage on, recruit for, and pollinate this plant during a seasonal dearth of preferred food sources. Olfactory learning plays a key role in forager constancy and pollination, and we therefore tested the effects of acute and chronic TRP feeding on forager olfactory learning, using proboscis extension reflex conditioning. At concentrations of 0.5–10 µg TRP ml −1 , there were no learning effects of acute exposure. However, memory retention (1 h after the last learning trial) significantly decreased by 56% following acute consumption of 0.5 µg TRP ml −1 . Chronic exposure did not alter learning or memory, except at high concentrations (5 and 10 µg TRP ml −1 ). TRP concentrations in nectar may therefore not significantly harm plant pollination. Surprisingly, TRP slightly increased bee survival, and thus other components in T. hypoglaucum honey may be toxic. Long-term exposure to TRP could have colony effects but these may be ameliorated by the bees’ aversion to T. hypoglaucum nectar when other food sources are available and, perhaps, by detoxification mechanisms. The co-evolution of this plant and its reluctant visitor may therefore likely illustrate a classic compromise between the interests of both actors.
研究领域:
生态学
·
昆虫学
·
植物化学
✓ 已验证
DOI
高影响因子
Biomaterials Science
·
2020
·Vol. 8
·期 17
·4757-4766
·
IF 6.183
Jing Zhang,Dan Liu,Jiale Liu,Yanfeng Han,Haiyan Xu,Xigang Leng,Deling Kong,Lanxia Liu
Combined blockades of CTLA-4 and PD-1 can yield better overall complementary clinical outcomes than individual blockades, but the response rates are still relatively low. To investigate the anti-tumor effects of various combined strategies, we designed various spherical nucleotide nanoparticles (SNPs) loaded with CTLA-4 aptamer (cSNPs), PD-1 siRNA (pSNPs) or both (hybrid SNPs, or hSNPs). The results demonstrated that hSNPs could promote significantly stronger anti-tumor immune responses in a nonredundant fashion than the mixture of pSNPs and cSNPs (pSNPs & cSNPs). We reasoned that this is because all individual immune cells could receive both CTLA-4 and PD-1 blockades when they engulfed hSNPs, but it is much less likely that individual immune cells could receive both CTLA-4 and PD-1 blockades as many of them may not take both pSNPs and cSNPS from pSNPs & cSNPs. Further results revealed that the synergistic immune stimulatory effects of CTLA-4 and PD-1 blockades in the form of hSNPs were at least partly through regulating the immune suppressive function of both Tregs and TIM3+ exhausted-like CD8 T cells and allowing effector T cells to expand. This mechanism is not identical to earlier reported mechanisms of CTLA-4 and PD-1 blockades.
研究领域:
癌症免疫治疗
·
纳米技术
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2025
·
IF 9
Yiting Liu,Yibo Wei,Xurui Li,Ning Xie,Chenhe Yao,Qiang Weng,Xingming Ning,Zhongwei An,Pei Chen,Xinbing Chen
Developing anion exchange membranes (AEMs) with simultaneous high ion conductivity and durability remains critical prerequisites for high performance fuel cell commercialization. Rational design of hydrophobic side chains has recognized as an effective approach to enhance ion transport in the AEMs through obvious microphase separation. Herein, we systematically design a series of AEMs with structurally tailored hydrophobic side-chain engineering strategy to boost both conductivity and stability. Moreover, the effects of hydrophobic side chain types on the AEM performance were thoroughly contrasted through a comprehensive investigation. Among them, the membrane 6BHC-FPhe-TMA incorporating mono-fluorine substituted alkoxy-phenyl hydrophobic side chains exhibit well-defined microphase separation, enabling exceptional high hydroxide conductivity of 161.06 mS cm −1 at 80 °C with low ion exchange capacity (1.37 mequiv. g −1 ) while maintaining controlled swelling ratio (14.08 %). Remarkable alkaline stability is evidenced by 93.24 % conductivity retention after 1344 h alkaline treatment (2 M NaOH, 80 °C). When integrated into H 2 /O 2 fuel cell, the membrane delivers a peak power density of 522 mW cm −2 and reveals outstanding durability with negligible voltage decay (0.16 mV h −1 ) during 72 h operating at 100 mA cm −2 . This work provides a novel materials design paradigm combing fluorine substituted alkoxy-phenyl side chain, offering significant progress toward practical AEMFCs applications.
研究领域:
材料科学
·
电化学
·
高分子化学
·
能源技术
✓ 已验证
产品关联
DOI
高影响因子
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
·
2026
·
IF 2.2
Ying Zhu,Longtao Zhu,Yue Lan,Chuan Sun,Guangdi Chen
Radiofrequency electromagnetic fields (RF-EMF) are widely present in the modern environment and have been classified by the International Agency for Research on Cancer as possibly carcinogenic to humans (Group 2B). However, their potential role as co-carcinogens remains unclear. The present study aimed to evaluate whether 1800 MHz RF-EMF exposure can modulate chemically induced DNA damage. Mouse embryonic fibroblasts (MEF) were exposed to RF-EMF alone or in combination with hydrogen peroxide (H 2 O 2 ), 4-nitroquinoline-1-oxide (4NQO), cadmium (Cd 2+ ), or hexavalent chromium [Cr(VI)]. RF-EMF exposure was performed using a waveguide system under standardized, non-thermal conditions, and DNA damage was assessed using the alkaline comet assay. RF-EMF exposure alone did not induce detectable DNA damage, nor did it significantly enhance DNA damage caused by H 2 O 2 , 4NQO, or Cd 2+ . In contrast, co-exposure to RF-EMF and Cr(VI) resulted in a significant synergistic increase in DNA damage in MEF cells. These findings suggest that RF-EMF may selectively exacerbate Cr(VI)-induced genotoxicity, highlighting the need for further investigation into the underlying co-toxic mechanisms.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOID JOURNAL
·
2011
·Vol. 73
·期 1
·12-17
·
IF 0.622
Wang Yuhui,Ma Qianqian,Liu Li,Miao Jigen,Miao Yuqing
A novel technique of enzyme immobilization is developed for making the immobilized enzymes capable of further releasing for homogeneous reaction. Water-soluble poly(vinyl alcohol) was used to prepare enzyme-loaded membranes with immobilized acetylcholinesterase (AChE). When used, a piece of enzymecontaining membrane is put into the solution and dissolves quickly. The released AChE is mixed and interacts with substrates and carbaryl inhibitors. The catalytical activity and inhibition sensitivity of released enzyme are comparable to those of free AChE. The values of Michaelis constant and maximum reaction rate for released AChE are also very close to those for free AChE. The experimental conditions such as the concentrations of PVA and acetone, the time of enzymatic reaction and that of AChE inhibition by carbaryl pesticide were optimized. The relative inhibition of AChE activity increased with the carbaryl concentration ranging from 0.1 μg/L to 100 mg/L. When compared to free AChE in solution or solid powder, the prepared PVA-AChE membranes are advantageous with respect to storage and handling. The suggested technique of enzyme immobilization is suitable for the variety of applications, when the enzyme catalysed reactions allows for single-using of the active material and does not require further enzyme recovering.
✓ 已验证
DOI
高影响因子
International Journal of Pharmaceutics-X
·
2025
·
IF 6.4
Ye Tao,Xiaohui Cai,Zhongping Chen
Cancer nanomedicines have shown great potential in fighting against cancer. While the development of cancer nanomedicines is advancing rapidly, preclinical assessment approaches for their therapeutic potency have stagnated. In view of high prevalence of cancer pain in cancer patients, we aim to determine whether therapeutic potency of a cancer nanomedicine can be predicted by pain-related behavioral test in subcutaneous tumor model, the simplest and most widely used tumor model in oncology. Behavioral profiles reveal that subcutaneous tumor, probably irrespective of tumor type, presents with spontaneous pain (open field test) and evoked pain (von Frey test for mechanical allodynia; Hargreaves test, hot plate test, and tail flick test for thermal hyperalgesia; cold plate test and acetone drop test for thermal allodynia). Using doxorubicin (DOX)-loaded lipid nanoparticles (LNPs) (LNPs/DOX) as a representative cancer nanomedicine and ropivacaine (ROP)-loaded LNPs (LNPs/ROP) as a pain nanomedicine, it is validated that inhibiting subcutaneous tumor growth can relieve cancer pain, while delaying the growth cannot, despite a significant difference found compared with non-treatment group. Moreover, behavioral results in all the tests are consistent and von Frey test is suggested the most sensitive among them. It is strongly suggested that pain-related behavioral test can serve as a powerful tool to predict therapeutic potency of a cancer nanomedicine in vivo in treating subcutaneous tumor.
研究领域:
肿瘤学
·
纳米医学
·
疼痛研究
·
药理学（临床前）
·
行为神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2024
·
IF 3.7
Hao Xu,Ning Ning Guo,Chen Ying Zhu,Lin Yan Ye,Xing Yi Yan,Yong Qin Liu,Ze Yan Zhang,Guangji Zhang,Liaqat Hussain
Diterpenoid tanshinones (DTs) are a bioactive fraction extracted from Salvia miltiorrhiza. High-performance liquid chromatography analysis revealed the presence of four compounds, namely, tanshinone IIA, tanshinone I, cryptotanshinone, and dihydrotanshinone. In this study, we aimed to propose a possible mechanism for the anti-lung cancer effect of DT. To do so, we utilized a lung cancer nude mice model and a lung cancer cell line (PC9) to investigate the effect of DT on lung cancer. We employed immunohistochemistry, enzyme-linked immunosorbent assay, hematoxylin and eosin staining, and immunofluorescence to analyze the pharmacological role of DT in the inhibition of lung cancer growth. The results showed that DT inhibited tumor growth, induced apoptosis in the nude mice model, and reduced inflammatory cell infiltration. Additionally, DT inhibited PC9 lung cancer cells, growth, proliferation, and migration. The mechanism of action of DT involves not only directly inhibiting cell proliferation and migration but also improving the tumor microenvironment. DT significantly increased the expression of important intestinal gap junction proteins, such as zonula occludens 1 (ZO-1) and occludin I. This upregulation contributes to the reinforcement of the intestinal mucosal barrier, thereby reducing the paracellular transport of lipopolysaccharides (LPS) through the intestine. Consequently, the decreased LPS levels lead to the inhibition of NF-κB expression and downregulation of macrophage polarization, as indicated by the decreased expression of CD68. In conclusion, this study has confirmed that DT has anti-lung cancer properties by improving the inflammatory tumor microenvironment via regulating macrophage polarization and inhibiting LPS-associated immune response. These results provide new insights into the mechanism of DT action against lung cancer.
研究领域:
肿瘤学
·
药理学
·
免疫学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Yuan Yang,Yao Wang,Xiaoling Hou,Yao Lin,Lu Yang,Xiandeng Hou,Chengbin Zheng
Despite of increased interest in the application of miniature microplasma atomic spectrometry for environmental analytical chemistry , the amenable element detection range is limited to some metal elements and carbon due to it low power consumption. In this work, the generation of silicon atomic emission (251.6 nm and 288.2 nm) from the organosiloxanes was found possible in a low-temperature, low-power, and compact point discharge. Consequence, a tiny point discharge silicon optical emission spectrometer (μPD-OES) was exploited, and used as a novel GC detector for the determination of various cyclic volatile methyl siloxanes (cVMSs). Under the optimized conditions, the developed system provided limits of detection (LODs) of 0.2 mg L −1 , 0.04 mg L −1 , 0.03 mg L −1 and 0.02 mg L −1 of Si for hexamethylcyclotrisiloxane, octamethylcyclotetrasiloxane , decamethylcyclopentasiloxane and dodecamethylcyclohexasiloxane, respectively. Meanwhile, relative standard deviations (RSDs) of better than 2.3% were obtained. In contrast to gas chromatography mass spectrometer , GC-μPD-OES significantly simplifies the experimental setup with low power consumption and a miniature configuration. As far as we know, this work reports for the first time that silicon atomic emission can be generated in such low temperature microplasma. The accuracy of this system was validated by determining cVMSs in five daily-used shampoo samples collected from retail store, providing satisfactory recoveries (84%–114%) and excellent agreement with values determined by GC-MS at the 95% confidence level.
研究领域:
环境分析化学
·
光谱学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Theranostics
·
2021
·
IF 11.556
Han Wu,Hao Xing,Meng-Chao Wu,Feng Shen,Yu Chen,Tian Yang
Background: Conventional therapeutic strategies for advanced hepatocellular carcinoma (HCC) remains a great challenge, therefore the alternative therapeutic modality for specific and efficient HCC suppression is urgently needed. Methods: In this work, HCC-derived extracellular vesicles (EVs) were applied as surface nanocarrier for sequential nanocatalysts GOD-ESIONs@EVs (GE@EVs) of tumor-specific and cascade nanocatalytic therapy against HCC. By enhancing the intracellular endocytosis through arginine-glycine-aspartic acid (RGD)-targeting effect and membrane fusion, sequential nanocatalysts led to more efficient treatment in the HCC tumor region in a shorter period of time. Results: Through glucose consumption as catalyzed by the loaded glucose oxidase (GOD) to overproduce hydrogen peroxide (H 2 O 2 ), highly toxic hydroxyl radicals were generated by Fenton-like reaction as catalyzed by ESIONs, which was achieved under the mildly acidic tumor microenvironment, enabling the stimuli of the apoptosis and necrosis of HCC cells. This strategy demonstrated the high active-targeting capability of GE@EVs into HCC, achieving highly efficient tumor suppression both in vitro and in vivo . In addition, the as-synthesized nanoreactor could act as a desirable nanoscale contrast agent for magnetic resonance imaging, which exhibited desirable imaging capability during the sequential nanocatalytic treatment. Conclusion: This application of surface-engineering EVs not only proves the high-performance catalytic therapeutic modality of GE@EVs for HCC, but also broadens the versatile bio-applications of EVs.
研究领域:
肿瘤学
·
纳米医学
·
分子生物学
·
癌症治疗
·
医学影像
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）