Advanced Healthcare Materials
·
2024
·
IF 10
Jiaying Zhang,Sifang Zhao,Yifan Zhou,Hongze Liang,Lingling Zhao,Hui Tan
Diabetic wound healing is hampered due to oxidative stress, exacerbated inflammation, and impaired angiogenesis in the wounds. A pH-sensitive antioxidant hydrogel based on carboxymethyl chitosan (CMCS), oligoprocyanidins (OPC), and oxide dextran (Oxd) is prepared to accelerate diabetic wound healing. The hydrogel network is formed via imine and hydrogen bonding interactions in the presence of hydroxyl, amino, and aldehyde groups, and deferoxamine (DFO) is incorporated into the hydrogel. The hydrogel shows pH-triggered drug release, a good antioxidant, and anti-inflammatory properties, and can promote tube formation and cell migration in vitro. Moreover, the hydrogel can accelerate wound healing in streptozotocin (STZ)-induced diabetic mice by regulating the inflammation environment with up-regulation of anti-inflammatory factors (IL-4 and IL-10) and down-regulation of pro-inflammatory factors (TNF-α and IL-6), and promoting angiogenesis with up-regulation of HIF-1α, VEGF, and CD31. Thus, the pH-sensitive antioxidant hydrogel provides a promising therapeutic strategy for diabetic wound healing.
研究领域:
生物材料学
·
医学
·
药理学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2024
·
IF 2.7
Junwei Yang,Si Zhou,Haijiao Zheng,Qiong Jia
One of the most crucial and prevalent post-translational modifications is the phosphorylation of proteins. The study and examination of protein phosphorylation hold immense importance in comprehending disease mechanisms and discovering novel biomarkers. However, the inherent low abundance, low ionization efficiency, and coexistence with non phosphopeptides seriously affect the direct analysis of phosphopeptides by mass spectrometry. In order to tackle these problems, it is necessary to carry out selective enrichment of phosphopeptides prior to conducting mass spectrometry analysis. Herein, magnetic chitosan nanoparticles were developed by incorporating arginine, and were then utilized for phosphopeptide enrichment. A tryptic digest of β-casein was chosen as the standard substance. After enrichment, combined with matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS), the detection limit of the method was 0.4 fmol. The synthesized magnetic material demonstrated great potential in the detection of phosphopeptides in complex samples, as proven by its successful application in detecting phosphopeptides in skim milk and human saliva samples.
研究领域:
生物化学
·
蛋白质组学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
NORDIC PULP & PAPER RESEARCH JOURNAL
·
2022
·Vol. 37
·期 2
·290-299
·
IF 1.595
Xuemei Wang,Yingping Qi,Yongfeng Shen,Hua Li
A new chitosan derivative (CS-CHO) was successfully synthesized based on chitosan (CS) and cyclohexene oxide (CHO). It proved that CHO was successfully grafted onto the amino group of CS by N-alkylation reaction through the characterization of FTIR, XRD and DSC-TGA. The mechanical properties (tensile strength, folding endurance, tearing strength), optical properties (whiteness, glossiness) of the CS-CHO coated paper sample were tested. The results showed that the mechanical properties of the paper treated with 10 % CS-CHO increased significantly without changing the appearance of the paper. The paper permanence (tensile strength, folding endurance, tearing strength and pH) of the treated paper samples tested by dry-heat aging test. The results showed that the permanence of the treated paper samples was significantly improved compared with the blank aged paper samples, which indicated that CS-CHO had the function of deacidification while reinforcing the paper samples, providing the possibility for deacidification and reinforcement in one-step method and playing a positive role in the paper preservation.
研究领域:
材料科学
·
化学
·
纸张保护
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DOI
高影响因子
CARBOHYDRATE RESEARCH
·
2025
·
IF 2.5
Peixuan Wu,Hao Zhuang,Yang Zhou,Gaobo Yu,Zhendong Zhao,Tawfik Khattab,Xianghui Wang,Yuanyuan Liu
Chitosan (CS) exhibits pH- and concentration-dependent antimicrobial activity, underscoring the need to develop CS-based materials with more stable and robust antibacterial performance. In this work, dimethyloctadecyl-(3-trimethoxysilylpropyl) ammonium (TSA) was chemically grafted onto the hydroxyl and amino groups of CS to synthesize TSA-CS derivatives. Two forms of microspheres—designated as GA-TSA-CS-12 and STPP-TSA-CS-12—were subsequently fabricated via emulsion cross-linking and ionic cross-linking methods, respectively. The resulting TSA-CS materials were thoroughly characterized by FT-IR, TGA, GPC, SEM, and XPS, confirming successful grafting, modified thermal properties, and altered molecular weight. Antibacterial evaluation revealed that TSA-CS-12 achieved high inhibition rates of 97.7% against E. coli and 98.7% against S. aureus , while the corresponding microsphere formulations maintained up to 93% antibacterial activity. These findings demonstrate that TSA-functionalized chitosan materials exhibit enhanced and more stable antibacterial performance, showing promising potential for biomedical applications.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 63
·36331-36337
·
IF 2.936
Huishang Yang,Shenggui Chen,Lei Liu,Chen Lai,Xuetao Shi
Phosphorylated biopolymers can induce mineralization, mimic the process of natural bone formation, and have the potential as scaffolds for bone tissue engineering. 2-Methacryloyloxyethyl phosphorylcholine (MPC), a low cytotoxicity phosphorus source, is mainly applied in vascularization and promoting blood compatibility and has been less researched for bone repair. In this study, phosphorylated methacrylamide chitosan (PMAC) hydrogel was prepared by mixing methacrylamide chitosan (MAC) and different mass of MPC with photoinitiator under UV irradiation. A series characterization tests showed that PMAC hydrogels were successful prepared and had a pretty good mineralization ability. Moreover, human fetal osteoblastic (hFOB) cells cultured on PMAC hydrogels exhibited not only highly viability but also the enhanced ALP activity and calcium deposition. The PMAC hydrogels have great potential in bone tissue engineering applications.
研究领域:
材料科学
·
生物医学工程
·
组织工程
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DOI
PubMed
高影响因子
POLYMER
·
2020
·
IF 4.231
Shuang Sun,Yunming Wang,Longhui Li,Zhigao Huang,Huamin Zhou
Traditional fabrication methods for chitosan products limited in melt forming temperature higher than degradation temperature are hard to be directly applied to the manufacture of green transparent electronic substrates. Here, we proposed an innovative principle of all-green manufacture that surface molecular chains of chitosan nanospheres were melted at 120 °C and entangled under 8 MPa according to high molecules activity of Nano-effect on chitosan nanospheres surface, result in applicable mechanical performances (Hardness, 0.27 GPa; Modulus, 3.83 GPa). The optimum optical performance of chitosan substrate was 58% at visible light (400 nm ~ 800 nm). Additionally, chitosan nanospheres had been successfully fabricated as substrates of interdigital electrode sensors. Therefore, our innovative strategy provides a new processing concept of fundamental scientific principle, and directly promote the industrialization of chitosan in biosensors, thin film transistors , and all solid state supercapacitors , etc.
研究领域:
材料科学
·
生物医学工程
·
纳米技术
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DOI
高影响因子
CELLULOSE
·
2021
·Vol. 29
·期 1
·233-243
·
IF 5.044
He Meng,Lu Tong,Jia Zhengtao,Tian Huafeng,Feng Mengna,Zhang Xinjiang,Zhang Meng,Wang Chengshuang,Zhao Yanteng,Qiu Jun
The development of novel biodegradable and ultraviolet (UV) shielding packaging materials has become a research hotspot, which could avoid “white pollution”. Chitosan is an ideal raw material for food packaging but lacks sufficient UV shielding property. In this work, we used an alkali/urea/zincate aqueous solvent to dissolve chitosan, and the resulting chitosan solution was heated with the existence of chemical crosslinker to convert Zn(OH) 4 2− into ZnO particles directly. Then, the chitosan/ZnO composite hydrogel sheets were obtained, which were converted to chitosan/ZnO composite bioplastic (CCZP) by a fixing and drying process. The structure and properties of CCZP were investigated with X-ray diffraction (XRD), scanning electron microscopy, XPS, water uptake and tensile testing etc. The results indicated that the in-situ synthesized ZnO particles had distributed evenly in the chitosan matrix. The optical transmittance of the obtained CCZP reached 0.01% at 300 nm and 51.6% at 800 nm, respectively, showing excellent UV shielding performance and relatively good visible light transmittance. Meanwhile, the mechanical properties of the chitosan bioplastic were improved by the incorporation of ZnO, and the tensile strength of CCZP reached up to 49.8 MPa, showing potential application as food packaging materials. Graphic abstract
研究领域:
材料科学
·
食品科学
·
高分子化学
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DOI
高影响因子
IRANIAN POLYMER JOURNAL
·
2016
·Vol. 25
·期 3
·277-284
·
IF 1.684
Li He-ping,Li Hui,Liu Yi
The development of new environment-friendly and efficient adsorbents has attracted a great interest in recent years. In this study, ethylene diamine-grafted chitosan copolymer (CS–MAA–EN) and triethylene tetramine-grafted chitosan copolymer (CS–MAA–TN) were synthesized to remove heavy metal ions from water. The influence of pH, adsorbents dosage and initial metal concentration were investigated to study the adsorbing effect of CS–MAA–EN and CS–MAA–TN for the removal of Cu 2+ from aqueous solutions. The equilibrium adsorption capacities of CS–MAA–EN and CS–MAA–TN were 85.91 and 102.67 mg/g, respectively. The adsorption process was fitted better by the Langmuir isotherm model ( R
2 = 0.9993, 0.9991) than the Freundlich isotherm model ( R
2 = 0.8781, 0.8775). The adsorption kinetics confirmed that the adsorption mechanism could be better described by the pseudo-second-order equation. Two adsorbents showed excellent desorption efficiency ( D
e ) and reuse ratio ( R
u ). D
e and R
u of CS–MAA–EN were evaluated as 95.2 and 89.35 %, respectively, and those values of CS–MAA–TN were 92.73 and 83.25 %. The competitive adsorption results of the two adsorbents indicated that the rate sequence was Fe 3+ > Cu 2+ > Cr 6+ > Ni 2+ > Zn 2+ .
研究领域:
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
MATERIALS & DESIGN
·
2024
·
IF 7.6
Yifan Lu,Ze-Yu Wang,Jianhong Xiao,Zhengyi Zhao,Xiaoyu Liao,Huai-Ling Gao,Duohong Zou
Treatment of osteochondral (OC) defects is still an immense challenge in clinic due to lack of satisfactory strategy to simultaneously regenerate both cartilage and subchondral bone. Herein, we propose an effective bottom-up strategy by developing a type of multifunctional chitosan-based nanocomposite microgels as the building blocks to repair OC defects. The microgels are biocompatible, and biodegradable, and have a hierarchical nanofibrous structure, mimicking the structural feature of extracellular matrix (ECM) to facilitate cell attachment and proliferation. Additionally, β-tricalcium phosphate (β-TCP) nanoparticles are incorporated in the microgels not only to promote the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), but also to facilitate the loading of transforming growth factor beta 1 (TGF-β1). For reconstructing OC defects, pure microgels are implanted at the bottom layer of the OC defects in rat knee joints to promote osteogenesis, while the microgels loaded with TGF-β1 are implanted at the top layer of the OC defects to promote cartilage regeneration. This modular microgel system results in simultaneous regeneration of cartilage and subchondral bone in OC defects, which is expected as a promising way for repairing other complex tissue defects in the field of tissue engineering.
研究领域:
组织工程
·
生物材料
·
再生医学
✓ 已验证
DOI
高影响因子
CURRENT PHARMACEUTICAL BIOTECHNOLOGY
·
2023
·
IF 2.8
Chang Chun-hui,Han De-en,Ji Yu-ying,Wang Meng-yan,Li Dong-hong,Xu Zhi-ling,Li Jia-hao,Huang Sheng-nan,Zhu Xia-li,Jia Yong-yan
Background: Although quercetin exhibits promising anti-tumor properties, its clinical
application is limited due to inherent defects and a lack of tumor targeting.
Objective: This study aimed to prepare and characterize active targeting folate-chitosan modified
quercetin liposomes (FA-CS-QUE-Lip), and its antitumor activity in vitro and in vivo was also
studied.
Methods: Box-Behnken Design (BBD) response surface method was used to select the optimal
formulation of quercetin liposomes (QUE-LP). On this basis, FA-CS-QUE-LP was obtained by
connecting folic acid chitosan complex (FA-CS) and QUE-LP. The release characteristics in vitro
of QUE-LP and FA-CS-QUE-LP were studied. Its inhibitory effects on HepG2 cells were studied
by the MTT method. The pharmacokinetics and pharmacodynamics in vivo were studied in
healthy Wistar mice and S180 tumor-bearing mice, respectively.
Results: The average particle size, zeta potential and encapsulation efficiency of FA-CS-QUELP
were 261.6±8.5 nm, 22.3±1.7 mV, and 98.63±1.28 %, respectively. FA-CS-QUE-LP had a
sustained release effect and conformed to the Maloid-Banakar release model (R2=0.9967). The
results showed that FA-CS-QUE-LP had higher inhibition rates on HepG2 cells than QUE-Sol
(P<0.01). There was a significant difference in AUC, t1/2, CL and other pharmacokinetic parameters
among QUE-LP, FA-CS-QUE-LP, and QUE-Sol (P<0.05). In in vivo antitumor activity
study, the weight inhibition rate and volume inhibition rate of FA-CS-QUE-LP were 30.26% and
37.35%, respectively.
Conclusion: FA-CS-QUE-LP exhibited a significant inhibitory effect on HepG2 cells, influenced
the pharmacokinetics of quercetin in mice, and demonstrated a certain inhibitory effect on S180
tumor-bearing mice, thus offering novel avenues for cancer treatment.
研究领域:
药学
·
肿瘤学
·
药物递送系统
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2021
·
IF 8.742
Qingqing Guo,Xin Wu,Yanhong Ji,Yufan Hao,Shuyu Liao,Zhenyu Cui,Jianxin Li,Mohammad Younas,Benqiao He
Nanofiltration (NF) membranes with adjustable permeability and selectivity are highly desired for the separation of small molecules. A novel pH-responsive NF membrane was synthesized via interfacial polymerization with a mixture of piperazine (PIP) and chitosan (CS) as the aqueous phase and trimesoyl chloride (TMC) in n-hexane as the organic phase. The effects of pH on the physicochemical structure and performance of the PIP-CS/TMC NF membrane obtained were studied in detail. The results showed that the surface physicochemical structure and performance of the membrane could be changed by adjusting the pH of feed solution. Specifically, the surface roughness, hydrophilicity and pore size of the NF membrane were increased at high pH values. The pure water flux through the NF membrane remained stable in the pH range 2–8 but increased exponentially from 248.2 to 643.8 L m −2 ·h −1 as the pH was increased from 8 to 12. Furthermore, the PIP-CS/TMC NF membrane demonstrated more than 99.0% rejection for Congo red, methyl blue, coomassie brilliant blue G250 in the pH range 2–12, while low rejection was observed for methyl orange and inorganic salts (Na 2 SO 4 , MgCl 2 ) at pH 12. The NF membrane exhibited excellent separation performance of the mixtures of anionic dye/salt or dye/dye with different molecular weights at pH 12. The reversibility of pH response with a high responsive coefficient of the NF membrane was also proved. This work provides a new route for NF membrane in the separation of some dye/salt and mixed dyes systems.
研究领域:
材料科学
·
环境工程
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Jie Ren,Zengyang Wu,Bai Wang,Liuping Zheng,Siyu Han,Jianshe Hu
Chitosan-based hydrogels, as natural high-molecular-weight flexible materials, are widely utilized due to their outstanding properties. In this research, we developed a one-pot method for synthesizing a novel PVA/CS@PPy-PDA x% conductive hydrogel and explored the internal bonding patterns through molecular dynamics simulations. By adding PPy-PDA nanoparticles into a hydrogel matrix, an interpenetrating conductive network established successfully. The uniform distribution of PPy-PDA nanoparticles endowed the hydrogel with good electrical conductivity (0.171 S/m), significantly enhanced mechanical properties, and strain sensing (S = 5.04), as well as near-infrared photothermal responsiveness (temperature increase of 41.9 °C within 30 s). Additionally, due to the hydrogel's significant photothermal conversion efficiency under near-infrared radiation, it exhibits rapid elimination of Escherichia coli with an antibacterial efficiency exceeding 90 %. The unique hydrogen-bonded crosslinked structure provides the hydrogel with excellent re-healing properties, allowing for restoration through a freeze-thaw process after damage. The conductivity remains nearly unchanged after re-healing, maintaining the material's integrity and functionality. The flexible sensor based on this hydrogel has a response time of 100 ms and can sensitively detect large-scale deformations (e.g., joint bending at various angles), different gravitational forces, and recognize human handwriting. These characteristics make this hydrogel a promising candidate for advancing intelligent wearable technologies and human-machine interaction systems.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）