TOXICOLOGY LETTERS
·
2024
·
IF 2.9
Yingxin Pang,Xiaoqi Tao,Zongmin Qin,Muran Jiang,Erqun Song,Yang Song
This work investigated the influence of surface chirality on cellular internalization, cytotoxicity, and tissue distribution of silver nanoparticles (AgNPs). D-cysteine and L-cysteine are chiral forms of the amino acid cysteine. These enantiomers exhibit distinct spatial arrangements, with D-cysteine having a different configuration from L-cysteine. This structural dissimilarity can lead to variations in how these forms interact with biological systems, potentially impacting their cytotoxic responses. Four distinct types of AgNPs were synthesized, each possessing a unique surface coating: pristine AgNPs (pAgNPs), L-cysteine coated AgNPs (AgNPs@L-Cys), D-cysteine coated AgNPs (AgNPs@D-Cys), and racemic AgNPs coated with both L-Cys and D-Cys (AgNPs@L/D-Cys). We found chiral-dependent cytotoxicity of AgNPs on J774A.1 cells. Specifically, AgNPs@L-Cys exhibited the highest toxicity, and AgNPs@D-Cys exhibited the lowest toxicity. Meanwhile, the cellular uptake of the AgNPs correlated nicely with their cytotoxicity, with AgNPs@L-Cys being internalized to the greatest extent while AgNPs@D-Cys displays the least internalization. Scavenger receptors and clathrin predominantly mediate the cellular internalization of these AgNPs. Strikingly, the dissimilar cellular internalization and cytotoxicity of AgNPs with different chirality were eliminated upon protein corona coverage. Notably, following intravenous injection in mice, these four types of AgNPs showed similar patterns among various organs due to the inevitable protein adsorption in the bloodstream. These findings underscored the pivotal role of surface chirality in governing the biological interactions and toxicity of AgNPs.
研究领域:
纳米技术
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毒理学
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生物材料科学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2025
·
IF 5.1
Jing Zhang,Ye Yang,Yi-Ping Liu,Guo-Cong Liu,Xi Zhu,Xin-Yu Jiang,Jin-Gang Yu
Electrochemical sensors have been considered as one of the most important methods to determine amino acid (AA) enantiomers. An interesting and highly-efficient electrochemical sensor, gold nanoparticles/D-sorbitol modified glass carbon electrode (Au NPs/D-sorbitol/GCE), was developed for the chiral recognition of Tyrosine (Tyr) enantiomers. Au NPs/D-sorbitol/GCE sensor exhibited excellent chiral recognition efficiency for Tyr enantiomers (I L /I D = 1.99, ∆Ep = 36 mV) by square wave voltammetry (SWV). The possible recognition mechanism was also proposed by cyclic voltammetry (CV) at different scanning rates, wettability measurements, and density-functional theory (DFT) calculations. The smaller steric hindrance and more suitable molecular distance are beneficial to the selective formation of hydrogen bonds between L-Tyr and the chiral interface of the sensor. The energy difference caused by the formation of hydrogen bonds between the chiral interface and Tyr enantiomers can be expressed as different peak currents to achieve chiral recognition. The redox peak current of Tyr enantiomers at Au NPs/D-sorbitol/GCE showed good linear relationship with their concentration, and the limits of detection (LOD) were calculated to be 4.12 μM and 8.07 μM for L-Tyr and D-Tyr, respectively. Importantly, Au NPs/D-sorbitol/GCE can also predict the mass ratios of L-Tyr in the enantiomeric mixtures. In addition, the sensor has shown good anti-interference ability, high stability, and excellent reproducibility.
研究领域:
分析化学
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电化学
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生物传感
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手性分析
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纳米材料
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分子识别
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氨基酸检测
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
·
IF 6.2
Yuxin Wang,Zijian Tan,Hongli Wei,Nan Zhang,Lifei Wang,Yifan Yang,Weidong Liu,Leilei Zhu
D-Tagatose, a promising sugar substitute with various functional properties and commercial applications, can be enzymatically converted from D-fructose by tagatose 4-epimerase. The development of an efficient tagatose 4-epimerase that catalyzes the conversion of D-fructose into D-tagatose is essential to make the production technology of D-tagatose applicable. In this study, tagatose 4-epimerase from Thermotogae (TsT4Ease) was engineered through semi-rational design and directed evolution, resulting in a 2.8-fold improvement in catalytic activity compared to the wild type (WT). The production of D-tagatose reached 42 g/L in 2 h. Crystal structure analysis determined the structural features with a common (α/β)8-TIM barrel and a Zn2+-binding architecture at the active center. Subsequent molecular dynamics (MD) simulations revealed that the substitutions improved substrate binding energy and stabilized the active pocket. This study offers new insights into the structure–function relationship of TsT4Ease and provides a candidate tagatose 4-epimerase.
研究领域:
生物化学
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蛋白质工程
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结构生物学
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DOI
PubMed
高影响因子
Cell Death & Disease
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2025
·Vol. 16
·期 1
·1-11
·
IF 9.6
Chu Xiufeng,Zhang Ting,Bukhari Ihtisham,Hu Mei,Xu Jixuan,Xing Yamin,Liang Xinfeng,Zhang Zisen,Zheng Pengyuan
Gasdermin D (GSDMD) is a critical pyroptosis mediator, consisting of one N-terminal pore-forming domain and one C-terminal auto-inhibitory domain. The free N-terminal domain (GD-NT), which is released through caspase-1/11 cleavage, exhibits distinct features from the full-length GSDMD (GD-FL), including oligomerization, membrane translocation, and pore-formation. However, the underlying mechanisms are not well elucidated. Here, we found that GD-NT, but not GD-FL, was massively ubiquitinated in cells. The K63-linked polyubiquitination of GD-NT at Lys236/237 (human/mouse), catalyzed by TRAF1, directly prompted its membrane translocation and pore-formation during pyroptosis. Inhibition of GD-NT ubiquitination via site-directed mutations or the UBA1 inhibitor PYR-41 suppressed cell death in several pyroptosis cell models. Additionally, applying PYR-41 in septic mice efficiently suppressed the release of IL-18 and TNFα. Thus, GD-NT ubiquitination is a key regulatory mechanism controlling its membrane localization and activation, which may provide a novel target for modulating immune activity in pyroptosis-related diseases.
研究领域:
免疫学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
Food & Function
·
2021
·Vol. 12
·期 5
·2148-2160
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IF 5.396
Yaojie Liu,Ying Liu,Yatu Guo,Lin Xu,Hao Wang
Phlorizin is the main active ingredient of apple peel and has potential utilization value. Some recent studies have suggested that phlorizin may have antioxidant capacity and protect the liver. The injection of a low dose of D-galactose can cause some changes that resemble accelerated aging in mice. This study explored the protective effects of phlorizin on D-galactose-induced mice and PC12 cells. In this study, ICR mice were divided into a normal group (NOR), a D-galactose model group (D-gal) and phlorizin treatment groups (100 mg kg−1, 200 mg kg−1 and 400 mg kg−1). In addition to the NOR group, four other groups were injected with D-galactose (120 mg kg−1) for 12 weeks. The results showed that phlorizin reduced the decline of strength, coordination and spatial memory caused by aging, increased the activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px), increased total antioxidant capacity (T-AOC), and reduced the content of malondialdehyde (MDA). On the other hand, phlorizin increased the levels of interleukin-2 (IL-2) and acetylcholine (ACh), reduced the release of interleukin-6 (IL-6), aspartate aminotransferase (AST) and alanine aminotransferase (ALT), and decreased the activity of acetylcholinesterase (AChE) in the brain, improved the expression of antioxidant genes related to the nuclear factor E2-related factor 2 (Nrf2) pathway, and reduced the occurrence of morphological lesions in the hippocampus and liver. In addition, phlorizin improved cell viability and reduced the cytotoxicity of D-galactose-induced oxidative stress in PC12 cells. Meanwhile, the protective effect of phlorizin was abolished in Nrf2 gene knockdown PC12 cells. Furthermore, molecular docking showed that phlorizin could bind Keap1 protein, which can interact with Nrf2 protein. Therefore, these results suggest that phlorizin may delay senescence and enhance antioxidant capacity through the Nrf2 pathway.
研究领域:
老年学
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药理学
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分子生物学
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DOI
PubMed
高影响因子
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
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2017
·
IF 2.962
Ee Li,Jiajia Wu,Dun Zhang,Yan Sun,Juna Chen
D-phenylalanine (D-Phe) was tested for its effect on the corrosion of Q235 carbon steel caused by Desulfovibrio sp. which belongs to sulfate reducing bacteria (SRB). Weight loss results indicated that the corrosion rate of Q235 coupons exposed to D-Phe was reduced. Much less pits were found on the surface of these coupons. It was found that pH and dissolved oxygen content of bacteria cultures as well as the growth of bacteria were not affected by D-Phe. However, the cell shape of D . sp. was changed to a more filamentous or spherical shape by treatment with D-Phe, and the structure of biofilms formed by bacteria with transformed shapes was looser and thinner. Moreover, the hydrogen sulfide production of D . sp. was inhibited by D-Phe. These results suggest that D-Phe may inhibit the corrosion process through two ways, i, D-Phe alters the bacteria shape, which inhibits the formation of biofilm and changes the structure of it consequently; ii, D-Phe reduces the production of hydrogen sulfide .
研究领域:
微生物腐蚀
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材料科学
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环境微生物学
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金属腐蚀与防护
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生物膜研究
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DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2024
·
IF 5.3
Jiancheng Zhao,Zhaohui Guo,Christian Marcus Pedersen,Lingyu Jia,Shiyu Jia,Xianglin Hou,Yingxiong Wang,Yan Qiao
Organonitrogen chemicals are widely used and in great demand in the food, pharmaceutical and chemical industries. Hence, it is important to develop green and efficient synthesis paths to organonitrogen chemicals for their use as new resources. Herein, we propose the preparation of organonitrogen chemicals from chitin biomass monomer N -acetyl- d -glucosamine (GlcNAc) promoted by natural deep eutectic solvents (NADESs). The selective generation of 3-acetamido-5-(1′,2′-dihydroxyethyl) furan (Chromogen III) and 3-acetamido-5-acetylfuran (3A5AF) from GlcNAc was achieved by the design of NADESs components. GlcNAc reacts in binary NADES proline-glycerol (Pro-Gly) to generate 26 % of Chromogen III and 39 % of 3A5AF in ternary NADES proline-glycerol-lactic acid (Pro-Gly-LA). In addition, the intermolecular hydrogen bonds in NADESs were confirmed through 1 H diffusion-ordered spectroscopy (DOSY) NMR technology. And the results of 13 C NMR chemical shift titration indicated interaction between NADESs and the C3 and C6 position of GlcNAc. Based on the NMR experiments a mechanism is proposed.
研究领域:
生物化学
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绿色化学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Xiaoying Gao,Jiarui Zhu,Jiahui Zhao,Lei Zhao,Ying Sun,Jiazhen Lin,Mengli Hu,Yunjia Liu,Shenghong Yang,Jian Liu
A cascade colorimetric detection of salivary D-amino acids (DAAs) holds promise for the preliminary screening diagnosis of gastric cancer. Pursuing metal-organic complexes with high peroxidase-mimic (POD-mimic) activity is crucial to enhance diagnosis efficiency. In this work, we developed a straightforward strategy in a “one-pot” process to modulate the POD-mimic activity of CuX-trithiocyanuric acid (CuX-TTCA) complexes. By adjusting the molar ratios of CuX (Cl, Br, and I) and TTCA during synthesis to modify the coordination configuration of Cu(I) in CuX-TTCA, we easily tuned their POD-mimic activities. Among them, CuCl-TTCA-2 with a feeding molar ratio of 2:1 for CuCl:TTCA exhibited remarkable POD-mimic activity attributed to its exposed catalytic active sites and efficient mass transfer ability during catalysis. Long-lived 1 O 2 was identified as the primary reactive oxygen intermediate. A highly sensitive and selective cascade colorimetric detection platform was developed for two typical DAAs associated with gastric cancer, namely D-proline and D-alanine, achieving limit of detection values of 1.1 and 0.8 μM, respectively. As a proof-of-concept application, our cascade detection platform demonstrated excellent specificity in distinguishing saliva samples between gastric cancer patients and healthy individuals. The outstanding selectivity and reliable outcomes from DAAs assay make our detection platform highly promising for preliminary screening diagnosis of gastric cancer and patient self-detection applications. Our straightforward strategy for tuning POD-mimic activity provides an in-depth understanding on structure-activity relationship in nanozymes, offering valuable opportunities to advance enzyme-mimic optimization for other potential applications.
研究领域:
生物化学
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医学诊断
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纳米酶研究
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产品关联
DOI
高影响因子
ENVIRONMENTAL TOXICOLOGY
·
2025
·
IF 3.2
Yang Li,Keyuan Zhong,Sijie Zhang,Shiyi Duan,Kaijie Huang,Xiaofang Che,Xinchun Guo,Huiqiang Lu
D-tetramethrin is a common insecticide that is important for the control of mosquito-transmitted diseases such as malaria and dengue and, due to its widespread use, it is frequently detected in environmental systems. However, there is currently very little information on the influence of D-tetramethrin on non-target organisms. In this study, zebrafish embryos were exposed to various concentrations of D-tetramethrin (0, 3, 6, and 9 mg/L) from 6 to 72 h past fertilization (hpf) to ascertain the influence of D-tetramethrin on the zebrafish immune system and development. We found that D-tetramethrin exposure led to a significant decrease in heart rate, an increase in the yolk area and tail flick frequency, and a shortening of body length in zebrafish larvae, compared with the control group. The number of macrophages and neutrophils in the experimental group of zebrafish larvae decreased significantly and the oxidative stress levels increased compared with the control group. The malondialdehyde (MDA) content and reactive oxygen species (ROS) content increased significantly, while catalase (CAT) activity increased and superoxide dismutase (SOD) activity decreased in the experimental group. At the same time, apoptosis increased in the zebrafish embryo cells, and the expression of apoptosis-related genes such as p53 , Bax , and Bcl-2 was abnormal. Moreover, the pro-inflammatory genes IL-8 , IFN-γ , IL-6 , and TNF-α were up-regulated, while the anti-inflammatory gene, IL-10 , was down-regulated. Therefore, D-tetramethrin significantly affects the immune system and oxidative stress levels in zebrafish, inducing cell apoptosis and negatively affecting embryo development. These results provide novel data for the toxicity of D-tetramethrin and the potential adverse effects of environment residues.
研究领域:
环境毒理学
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发育生物学
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免疫学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 15
·8823-8831
·
IF 3.108
Tsogzolmaa Ganbold,Gerile Gerile,Hai Xiao,Huricha Baigude
RNA interference (RNAi) has shown great potential for clinical treatment of a variety of diseases. As an effective post-transcriptional regulation, RNAi induces degradation of specific target mRNA, leading to a decreased level of disease related gene product, which could be resistant to conventional small molecule therapeutics. Because of the relatively small size of short interfering RNA (siRNA) as well as it's robustness in the design of efficient 20–22 nucleotide sequences targeting any known gene, delivery of siRNA in vitro and in vivo has achieved tremendous success by the design and fabrication of various carrier materials including peptides, lipids, and polymers. While siRNA delivery efficiency of these materials proved to be appreciable, their tissue specificity, biocompatibility and cytotoxicity still need much improvement. We report a novel siRNA delivery material designed by optimization of our previously reported peptidomimetic based lipoplex. The novel lipoplex, which bears a D-amino acid based dipeptide head group, possesses an ideal balance of increased stability in a tissue environment, and enhanced efficiency of tissue specific delivery as well as minimized cytotoxicity. The novel lipoplex carrying siRNA efficiently targeted mouse liver, initiated RNAi and knocked down the apoB dose dependently, which is confirmed by both RT-qPCR and Western blotting. Moreover, the novel lipoplex does not induce any apparent cytotoxicity, as confirmed by the measurement of liver enzyme levels in serum.
研究领域:
分子生物学
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药学
·
生物技术
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产品关联
DOI
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2024
·
IF 5.8
Liwei Liang,Songlin Cai,Lan Zhang,Ke Sun,Ziying He,Libin Zhang,Ciyuan Huang,Min Long,Hongxiang Zhu,Bingsuo Zou,Tao Liu,Shuangfei Wang
Phenol has various applications in industry, due to its hazardousness, solubility, and volatility, it persists in water and poses a risk to human health. People are excited by the progress of photodegradation technology that facilitates water pollution treatment. The effect of side-chain engineering is proven to enhance photodegradation performance by improving optoelectronic properties. Therefore, here is a strategy that introduces functional groups to change the electron cloud density, achieving excellent optoelectronic properties evidenced by efficient exciton dissociation, separation, and fine-tuned energy level. Subsequently, by coupling of photocatalysis and adsorption, the donor/acceptor (D/A) heterojunction photocatalysts (PBDB-T: BTTIC-TT, PBDB-T: BTTIC-Ph) are designed via loading on coconut-shell carbon to process phenol. The results indicate that the champion photocatalyst PBDB-T: BTTIC-TT possesses superior optoelectronic properties, which can be attributed to the stronger electron-withdrawing ability of thieno[3,2- b ]thiophene than benzene. Consequently, the synergistic efficiency of adsorption and photocatalysis of phenol (10, 20 mg/L) are removed within 10 mins over 90 % which is still valid after twenty cycles. Surprisingly, a high concentration of phenol (100 mg/L) also exhibited excellent removal efficiency of over 80 % within 60 min.
研究领域:
环境科学
·
材料科学
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产品关联
DOI
高影响因子
CHEMICAL PHYSICS LETTERS
·
2021
·
IF 2.328
Dongxin He,Wei Cao,Deshun Huang,Haimin Li,Jing Zhu,Pengxiang Zhao
Safety use of hydrogen leads to the development of highly sensitive materials for hydrogen sensing. Herein, Pd(II)@alkyne-PVA/ d -Ti 3 C 2 T x composite was prepared by spin-coating Pd(II)@alkyne-PVA solution onto the filter membrane supported with delaminated Ti 3 C 2 T x ( d -Ti 3 C 2 T x ). The properties of the composite were characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray (EDX) spectroscopy, and X-ray photoelectron spectroscopy (XPS). The results demonstrate that the composite shows high sensitivity and stability for hydrogen sensing.
研究领域:
材料科学
·
化学工程
·
纳米技术
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）