JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
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IF 5.7
Tong Zhou,Meigui Huang,Heping Cui,Shahzad Hussain,Khizar Hayat,Xiaoming Zhang,Chi-Tang Ho
The formation pathway and mechanism of various pyrazines were investigated during the thermal treatment of the alanine-xylose Amadori compound (Ala-ARP) and exogenous alanine (Ala). 15N-labeled Ala was used to coheated with Ala-ARP to clarify the nitrogen sources and the respective contributions of exogenous Ala and the regenerated Ala released from Ala-ARP to different pyrazine formation. It was found that exogenous Ala exhibited a priority in capturing glyoxal (GO) to form pyrazine during the thermal degradation of ARP. Compared to the Ala-methylglyoxal (MGO) model, a lower activation energy was required for the Ala-GO reaction, where the reaction dynamics of Ala-GO followed a zero-order model. In addition to forming pyrazine, the interaction between existing exogenous Ala and GO would accelerate the thermal degradation of Ala-ARP and retro-aldolization reaction of deoxyxylosones (DXs) to α-dicarbonyls. During this process, the release of regenerated Ala and MGO was promoted. Accordingly, as GO was expended by exogenous Ala during the initial stage of ARP-Ala degradation, the condensation between regenerated Ala and MGO became intensified, leading to the generation of methylpyrazine and 2,5-dimethylpyrazine. As a result, in the thermally treated mixture of Ala-ARP and exogenous Ala, 55% of the formed pyrazine originated from exogenous Ala, while 63% of the formed methylpyrazine and 57% of the formed 2,5-dimethylpyrazine were derived from regenerated Ala (120 °C, 30 min).
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2024
·Vol. 105
·期 1
·179-188
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IF 3.3
Linlu Zhang,Rong Bai,Shengqi Jiang,Ziwei Li,Ya Chen,Xiang Ye,Jiangtao Yu,Wu Ding
BACKGROUND Whey protein isolate (WPI) is a high-quality animal protein resource. The modification of WPI through physical, chemical and biological methods can substantially improve the functional properties of proteins. This study investigated the effect of electron beam irradiation (EBI) on the modification of WPI–xylose glycosylation. RESULTS The degree of grafting and browning revealed that EBI promoted WPI glycosylation. The maximum emission wavelength of intrinsic fluorescence was red-shifted and the fluorescence intensity was reduced, suggesting that irradiation induced the unfolding of the WPI structure, thereby promoting glycosylation. Fourier-transformed infrared spectroscopy revealed that the covalent binding of the conjugates occurred on the introduction of the hydrophilic groups, resulting in decreased surface hydrophobicity. When compared with conventional wet-heat glycosylation, irradiation-assisted glycosylation improved the emulsifying activity of WPI from 179.76 ± 0.83 to 277.83 ± 1.44 m 2 g −1 , and the emulsifying and rheological properties improved. CONCLUSION These results confirmed that EBI can increase the degree of WPI glycosylation and improve the functional properties of proteins, thereby laying a theoretical foundation for the further application of WPI. © 2024 Society of Chemical Industry.
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GREEN CHEMISTRY
·
2024
·Vol. 26
·期 4
·2170-2182
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IF 9.3
Zhuosen He,Yucui Hou,Jian Wei,Shuhang Ren,Weize Wu
Formic acid (FA) is a bulk chemical and a promising hydrogen energy carrier. The development of FA production from renewable biomass instead of multiple-step production from diminishing fossil resources is attractive. Formaldehyde (FAld) is another bulk chemical but industrial production requires high temperatures (250–600 °C). Herein, efficient oxidation of biomass to FA coupled with low-energy FAld production was developed using heteropoly acids as catalysts. Biomass and derivatives were oxidized to FA in yields of up to 88.6% while methanol was selectively oxidized to FAld at 90–190 °C with a markedly enhanced reaction rate. The synergistic mechanism has been unveiled that biomass activates methanol oxidation by boosting the catalytic redox cycle to form hydroxyl radicals and methanol scavenges the hydroxyl radicals to inhibit the overoxidation of biomass. This work opens up a promising direction for efficient FA production from biomass coupled with low-energy oxidation of alcohols to aldehydes.
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Journal of Cleaner Production
·
2024
·
IF 9.7
Haoran Wu,Xueli Chang,Yuli Wang,Xiaoyang Zheng,Chun Chang,Xiuli Han
This study provides an in-depth insight into the kinetics of corn cobs hydrolysis over formic acid to produce xylose and assesses the economic performance of the clean production process. The effects of reaction time, reaction temperature, and the solid-liquid ratio on the xylose yield were optimized by the response surface methodology. The results show that a high yield of xylose (29.9%) can be obtained under optimal conditions of a solid-liquid ratio of 1:10.15, 115.5 °C, and 66 min. The crystallization yield and purity of xylose reached 85% and 90%, respectively. Additionally, a mixed model consisting of a shrinking core model and a pseudo-first-order kinetics model was first proposed to elucidate the kinetics of corn cobs hydrolysis to xylose. Kinetic studies revealed that the depolymerization of lignocellulosic matrix to hemicellulose was the rate-determining step of the overall hydrolysis. Techno-economic analysis showed that the financial internal rate of return (FIRR) was 36.33% at the end of the project lifetime, which was much higher than the benchmark value of 15% for chemical enterprises. In addition, the investment profit margin was 52.11%, which was higher than the industry standard of 26%. The selling price of xylose was the main driving factor affecting FIRR.
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APPLIED BIOCHEMISTRY AND MICROBIOLOGY
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2024
·
IF 1
Hu J.,Wu Y.,Kang L.,Liu Y.,Wu Y.,Han D.,Wang J.
Abstract Shiga toxin-producing Escherichia coli (STEC) is an important foodborne pathogen that seriously threats the public and animal health. The major virulence of STEC is Shiga toxin (Stx) which is encoded by Stx prophage. Stx prophage induction leads to the production of Stx. Our recent research has shown that dietary functional sugars can differently regulate Shiga toxin type 2 (Stx2) prophage induction. However, high-throughput assays are required to find more dietary inhibitors for Stx prophage induction. This study aims to establish the recA dual-luciferase reporter system for rapidly screening the functional sugars that can inhibit Stx prophage induction. Results showed that the recA dual-luciferase reporter plasmid was successfully constructed as confirmed by DNA sequencing. Established recA dual-luciferase reporter system showed similar screening results when compared with traditional temperate phage determination method (i.e., qPCR) and suggested that L-arabinose is a dietary functional Stx prophage induction inhibitor. Supplementation with L-arabinose in culture medium reduces Stx production in STEC isolated from fecal samples of diarrhea piglets. Collectively, the recA dual-luciferase reporter system is a promising tool for convenient and rapid screening of dietary Stx prophage induction inhibitor, especially suitable for large-scale screening experiments.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Bin Ma,Jiafeng Niu,Hao Zhu,Huibing Chi,Zhaoxin Lu,Fengxia Lu,Ping Zhu
The oxidative reaction of Fusarium mycotoxin deoxynivalenol (DON) using the dehydrogenase is a desirable strategy and environmentally friendly to mitigate its toxicity. However, a critical issue for these dehydrogenases shows widespread substrate promiscuity. In this study, we conducted pocket reshaping of Devosia strain A6-243 pyrroloquinoline quinone (PQQ)-dependent dehydrogenase (DADH) on the basis of protein structure and kinetic analysis of substrate libraries to improve preference for particular substrate DON (10a). The variant presented an increased preference for substrate 10a and enhanced catalytic efficiency. A 4.7-fold increase in preference for substrate 10a was observed. Kinetic profiling and molecular dynamics (MD) simulations provided insights into the enhanced substrate specificity and activity. Moreover, the variant exhibited stronger conversion of substrate 10a to 3-keto-DON compared to the wild DADH. Overall, this study provides a feasible protocol for the redesign of PQQ-dependent dehydrogenases with favourable substrate specificity and catalytic activity, which is desperately needed for DON antidote development.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2024
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IF 8.1
Jiachang Shen,Maohua Yang,Jianmin Xing
Utilization of an in vitro multi-enzyme catalytic system for the synthesis of fructose from carbon dioxide is a promising approach towards achieving carbon neutrality. However, the lack of effective separation methods for the buffer HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) and the product fructose within the system has hindered the industrialization of in vitro multi-enzyme catalytic synthesis systems. This study aimed to separate and recover HEPES and fructose using nanofiltration technology. We investigated the influence of various parameters, including total solution concentration, solute concentration ratio, pH, and temperature, on the nanofiltration separation performance. Experimental results revealed that increasing the pH to 9.0 significantly decreased the fructose retention rate while maintaining a nearly complete HEPES retention rate. The HEPES-fructose mixed solution was further subjected to diafiltration using a spiral-wound membrane, ultimately yielding fructose and HEPES solutions with purities of 90.22 % and 92.93 %, respectively. Combining separation test results with models such as DSPM-DE, and using characterization methods such as ATR-FTIR and pore size distribution analysis, we observed pore swelling during nanofiltration of the HEPES-fructose solution at pH 9.0. A mechanism for this pore swelling, induced by the dissociation of HEPES under the influence of pH, was proposed: Dissociated HEPES in the solution interacts with the membrane surface at the liquid-membrane interface, causing reversible swelling of the membrane pores at the membrane surface, thereby reducing the retention rate of fructose. The nanofiltration process developed in this study effectively separates HEPES and fructose, enabling resource recycling and cost reduction. Furthermore, this study provides theoretical foundations and practical guidance for the design of nanofiltration separation strategies for similar systems using HEPES as a buffer, holding significant scientific and practical value.
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ChemistrySelect
·
2024
·Vol. 9
·期 11
·e202400114
·
IF 1.9
Jin Liang,Xiaoxin Wang,Dongsheng Yu,XiTao Luo,Xiangfeng Liang,Zhongliang Su,Qinghui Shou
Graphical Self-supported osmotic membranes were prepared by blending PIM-1-mPEG, which was obtained by modifying PIM-1 with polyethylene glycol monomethyl ether (mPEG), and hydrophilic Tröger's base polymer (DMBP-TB). The membranes utilized microporous molecular sieves and the differences in solute-membrane interactions to achieve high pentose/hexose selectivity in aqueous solutions. Monosaccharides are vital building blocks in bioengineering applications; however, their extraction from intricate mixtures is challenging and uses substantial amounts of energy. Polymers of intrinsic microporosity (PIMs) offer an innovative avenue for separating monosaccharides. We modified PIM-1membranes to improve the glucose/xylose separation by incorporating polyethylene glycol monomethyl ether (mPEG). The optimal mPEG (molecular weight: 1000 Da; mass fraction: 2.5 %; solvent: methanol) delivered a xylose separation coefficient of 2.62. With the hybrid membrane of PIM-1-mPEG (50 w.t.%) and hydrophilic Tröger's base polymerer (DMBP-TB, 50 w.t.%), the separation factor for xylose/glucose in an aqueous solution was 2.51 for single-stage running and 11.32 after five-stage running. There are large fractions of micropores for PIM-1-mPEG, and there is difference on solute-membrane interactions for pentose/hexose, which are regarded to be the main driving force for the high pentose/hexose selectivity in methanol. The blending of PIM-1-mPEG and DMBP-TB, integrates the microporosity and hydrophilicity, finally endues the high pentose/hexose selectivity in aqueous solution. These microporous membranes are promising materials for efficiently separating monosaccharides and jnl> small organic molecules while minimizing energy consumption. We established a solid foundation for further exploring microporous membranes for various applications, notably in bioengineering.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2024
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IF 9.4
Sijun Huang,Henglong Xiang,Jiachen Lv,Dongwei Zhu,Liqiang Yu,Yi Guo,Li Xu
As different monosaccharides exhibit different redox characteristics, this paper presented a novel colorimetric sensor array based on the glucose oxidase-like (GOx-like) activity of Au nanoparticles (NPs) for monosaccharides identification. AuNPs can use O 2 , ABTS +• , or [Ag(NH 3 ) 2 ] + as an electron acceptor to catalyze the oxidation of monosaccharides in different velocity, resulting in cross-responsive signals. The current sensor array can distinguish between different monosaccharides or their mixtures through linear discriminant analysis (LDA) and hierarchical clustering analysis (HCA). Moreover, the glucose and fructose concentrations can be estimated simultaneously using a neural network regression model based on the sensor array. This method shows potential for monosaccharide detection in industrial, medical, and biological applications.
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Pharmacognosy Magazine
·
2010
·
IF 0
Minxia Zheng,Jie Shen,Kai Yang,Songxiang Qian,Sujuan Feng
A simple approach for the rapid determination of polysaccharides in BianTi Soft Extract using spectrophotometry coupled with gas chromatography-mass spectrometry (GC-MS) was developed. The mixed standard solution composed of D-glucose, D-mannose, galactose and D-xylose in different proportions (1.00: 1.01: 0.12: 0.05) was prepared according to the monosaccharide composition analysis of the polysaccharides by GC-MS. The determination of polysaccharides by UV-Vis spectrophotometer was performed after 35-min color reaction, in which 1 ml 5% phenol and 4 ml sulfate was used. The assay of the method validation has shown that the method was stable, reliable and feasible. Furthermore, the proposed method was successfully applied in the preparation procedure of BianTi Soft Extract, selecting out optimal decoction conditions and suitable decoction container. It suggests that the convenient method could be useful for the quality control of BianTi Soft Extract. Meanwhile, it may be an alternative for polysaccharides determination of other formulations.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2012
·
IF 2.237
Shimin Kang,Xianglan Li,Juan Fan,Jie Chang
Hydrothermal carbonization of cellulose, lignin, d-xylose (substitute for hemicellulose), and wood meal (WM) was experimentally conducted between 225 and 265 °C, and the chemical and structural properties of the hydrochars were investigated. The hydrochar yield is between 45 and 60%, and the yield trend of the feedstock is lignin > WM > cellulose > d-xylose. The hydrochars seem stable below 300 °C, and aromatic structure is formed in all of these hydrochars. The C content, C recovery, energy recovery, ratio of C/O, and ratio of C/H in all of these hydrochars are among 63–75%, 80–87%, 78–89%, 2.3–4.1, and 12–15, respectively. The higher heating value (HHV) of the hydrochars is among 24–30 MJ/kg, with an increase of 45–91% compared with the corresponding feedstock. The carbonization mechanism is proposed, and furfural is found to be an important intermediate product during d-xylose hydrochar production, while lignin hydrothermal carbonization products are made of polyaromatic hydrochar and phenolic hydrochar. The formation of microspheres on the surface of cellulose and WM hydrochars is discussed, and transformation of the hemicellulose should be the reaction for WM microsphere production.
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Molecular Catalysis
·
2025
·
IF 4.9
Si Long,Zhi Chen,Xin Ju,Liangzhi Li
Isomerization cascade is a promising strategy to prepare valuable rare sugars from biomass xylose. However current conversions are not satisfactory due to factors of enzyme efficiency and reaction equilibrium. An isomerase/isomerase cascade has been newly developed employing an isomerase of xylose and a tagatose-3-epimerase (DTE) to prepare xylulose and ribulose from xylose, with a total xylose conversion of 43.2 %. In this study, two microbial glucose isomerases (GI) were tested as alternatives to isomerize xylose for their high activity towards xylose and thermal stability, aiming to explore the adaption effect of enzyme in the isomerization type of cascade. GIs from Streptomyces rubiginosus (SrGI) and Piromyces sp. (PsGI) both showed higher xylose conversions than the previously used isomerase of xylose, ribose-5-phosphate isomerase. The optimal reaction conditions of GIs for xylose conversion were compared in the single transformation of xylose and the cascade reactions catalyzed by GI/DTE, and it revealed the importance of high reaction temperature to substrate conversion and enzyme deactivation. The activity profile and thermal stability of SrGI were better than PsGI, making it a potential biocatalyst in biomass sugar isomerization which was favorable at high temperatures. The conversion of xylose by the PsGI/DTE cascade was improved to 71.3 % by efforts of enzyme adaption and reaction condition adjusting. Additionally, the standstill of isomerization reaction of monosaccharide can be attributed to thermal dynamic equilibrium at low temperatures and enzyme deactivation at high temperatures based on the results of this study.
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