Nano Today
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2022
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IF 18.962
Yuchun Guo,Jiangtao Ren,Erkang Wang
This work reports exquisite logic manipulation of nanometallic catalysis of DNA-templated silver nanoclusters (DNA-AgNCs) based on designative input-responsive DNA allostery and distinct inhibition effects of duplexes and triplexes on catalytic activity . A series of molecular logic gates (YES, OR, INHIBIT, XOR and MAJORITY) are presented, which are capable to control the catalytic activity of DNA-AgNCs in logic manners. Distinct from most of previous DNA-AgNC-based logic gates, its catalytic effect is adopted as the output signal instead of variable fluorescence , which offers a novel and robust approach for fabrication of molecular logic gate. One of the logic systems performs multiple bioanalysis of cardiac biomarkers (cardiac troponin I and myoglobin). Hence, it is worthwhile to anticipate intelligent detection of more significant disease biomarkers will be attained with the low-cost and universal platform. Moreover, the integration of programmable DNA reaction and aptamer recognition endows DNA-AgNCs catalytic specificity, which will give us hints how to devise smart DNA-functionalized nanomaterials .
研究领域:
生物化学
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纳米技术
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分子生物学
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催化科学
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DOI
高影响因子
Journal of Physical Chemistry C
·
2015
·
IF 4.772
Di Bao,Peng Gao,Yanbo Wang,Han Zhou,Yujin Chen,Guorui Chen,Piaoping Yang,Xitian Zhang
In this paper, Cd(OH)2 1D three-side nanobelt architectures have been fabricated through a facile two-phase reaction system. Considering their electropositive character proven by zeta potential measurement, the as-obtained Cd(OH)2 nanomaterials have been applied to capture negatively charged DNA. On the basis of the above results, the Cd(OH)2 nanomaterials have been employed for trapping negatively charged double-stranded DNA molecules (salmon sperm DNA). Assisted by the detailed observation of their DNA interaction results as well as through UV–vis spectroscopy, infrared imaging automatic target recognition (IRATR) and inductive coupled plasma atomic emission spectrometer (ICP-AES) measurements, it has been clearly shown that this 1D nanoarchitecture can effectively trap and release DNA molecules without use of any other organic reagents. In addition, a miniaturized electrochemical DNA-releasing device based on the Cd(OH)2 1D three-side nanobelt architectures has been fabricated. Using this device, it is possible to recycle the Cd(OH)2 nanostructures. In addition, the Cd(OH)2 nanomaterials maintain their nanostructures, and no Cd2+ ions were released in repeated DNA separation and transfer processes, which demonstrate their practicality for DNA trapping.
研究领域:
纳米材料科学
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生物物理学
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分子生物学
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DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2020
·
IF 6.182
Saijiao Yang,Meiying Liu,Fengjie Deng,Liucheng Mao,Yiyang Yuan,Hongye Huang,Junyu Chen,Liangji Liu,Xiaoyong Zhang,Yen Wei
Highly sensitive and selective detection of specific DNA or metal ions based on sensor platform of dye-labeled single-stranded DNA (named as ssDNA) and functional materials has attracted great research interest over the past few decades. Herein, we reported for the first time that polydopamine (PDA) modified SiO 2 nanoparticles (PDA- co -SiO 2 NPs) could be severed as effective fluorescent quenchers for fabrication of fluorescent DNA sensor systems for detection of functional nuclear acids and Ag + . To prepare PDA- co -SiO 2 NPs, we just need mix SiO 2 nanoparticles with dopamine in aqueous solution under rather mild experimental conditions. These PDA- co -SiO 2 NPs-based sensor systems possess excellent sensitivity and selectivity, fast response for detection of target ssDNA and Ag + based on different fluorescence quenching effect of PDA- co -SiO 2 NPs towards ssDNA and dsDNA. Furthermore, the high fluorescence quenching ability of PDA- co -SiO 2 NPs allows quantitative detection of target ssDNA with a low detection limit of 1 nM. More importantly, this method would also be easily extended for preparation of many other PDA-based materials for fabrication of fluorescent DNA sensors owing to the universal attachment of PDA with various materials and surfaces. Therefore, this work will open up a new direction for analytical detection of PDA-based functional materials.
研究领域:
纳米技术
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分析化学
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材料科学
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生物技术
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DOI
高影响因子
FOOD CHEMISTRY
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2019
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IF 5.399
Bin Wang,Jiushang Zheng,Ailing Ding,Liqun Xu,Jiuncun Chen,Chang Ming Li
A novel aptasensor, based on a perylene probe (PAPDI; N,N′-bis(propylenetrimethylammonium)-3,4,9,10-perylenediimide), was developed for the detection of aflatoxin B1 (AFB1) in maize samples. AuNPs/DNA composites were synthesized and integrated with aptamers-modified magnetic nanoparticles (MNPs) via DNA hybridization. For AFB1 determination, AuNPs/DNA composites were released from MNPs through specific binding of AFB1 with the aptamer and used for assembly of the PAPDI probe. To enhance the method sensitivity, terminal deoxynucleotidyl transferase (TdT)-catalyzed DNA polymerization was performed to elongate DNA on AuNPs/DNA composites. As a result, more PAPDI probes were assembled on the AuNPs/DNA composites. Through a multiple signal amplification strategy, the proposed method exhibited high sensitivity towards AFB1, with a detection limit of 0.01 nM (3.1 pg/mL). In summary, the proposed method has great potential to be a universal strategy for monitoring AFB1 in food samples.
研究领域:
食品科学
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分子生物学
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2013
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IF 5.437
Yunlei Zhou,Zhenning Xu,Mo Wang,Bing Sun,Huanshun Yin,Shiyun Ai
DNA methylation has important roles in gene regulation and relates to some diseases, especially cancers. Because DNA methylation is catalyzed by DNA methyltransferases (MTase), it is important to detect the activity of DNA MTase . In this work, we developed a novel visible light-activated photoelectrochemical (PEC) biosensor for DNA MTase activity assay, whereby bismuth oxyiodide (BiOI) nanoflake was synthesized as photoactive electrode material , M. SssI MTase as methylation reagent and methyl binding domain protein (MBD1 protein) as methylation recognition element. After cytosine methylation event occurred at the site of 5′-CG-3′, it could be probed by MBD1 protein and this protein could be combined tightly with methylated cytosine, which would lead to a decreased photocurrent due to the hindrance towards electron donor transferring to electrode surface by huge-volume protein. The decreased photocurrent was proportional to M. SssI MTase concentration from 0.1 to 50 unit/mL with the detection limit of 0.035 unit/mL (S/N=3). This detection limit was lower than that in some previous reports. This PEC biosensor showed high selectivity and good reproducibility for M. SssI MTase assay. Moreover, this method was successfully applied also to screen DNA MTase inhibitors, indicating that this PEC biosensor could be an alternative platform in anti-cancer pharmaceuticals discovery.
研究领域:
生物化学
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分子生物学
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癌症研究
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分析化学
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DOI
PubMed
高影响因子
ACS Applied Nano Materials
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2024
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IF 5.3
Mohamed Bahri,Zina Fredj,Peiwu Qin,Mohamad Sawan
Detecting preinvasive tumors before the onset of advanced clinical symptoms represents a critical advancement in improving the efficacy of early medical interventions and reducing cancer-related mortality rates. Immunosensors have emerged as the preferred choice for analyzing oncomarkers and meeting the ever-increasing demands of medical diagnostics. Therefore, the quest for the development of highly specific and sensitive immunosensors remains most sought-after to open new avenues for personalized healthcare and medical monitoring. Here, we report amplification-free and nonlabeled DNA framework-coupled AuNPs@CuMOF for ultrasensitive and highly specific electrochemical detection of carcinoembryonic antigen (CEA). Upon the CEA concentration variation from 0.0001 to 200 ng mL–1, the proposed biosensor led to a limit of detection (LOD) of 0.25 pg mL–1, surpassing most previously published CEA biosensors that rely on amplification-free methods and aligns with those based on amplification techniques. Furthermore, the suggested CEA immunosensor demonstrated auspiciously high specificity and sensitivity in detecting CEA in human serum comparable to the commercial ELISA within a relative error falling between 6.25% and 2.26%. The proposed analytical approach, hinging on the synergy effect of the CuMOF’s large surface area and the mechanical rigidity of the three-dimensional tetrahedron DNA structured probes (3D TDNA), obviously broadens the potential of immunosensors, offering higher sensitivity and lower LOD. Therefore, this work is not only an expansion of CuMOF exploitation in electrochemical biosensing but also a major milestone in detecting diverse biomarkers in clinical settings for tumor diagnosis.
研究领域:
生物医学工程
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分析化学
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医学诊断
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癌症研究
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生物传感器
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DOI
高影响因子
Analytical Methods
·
2020
·Vol. 12
·期 27
·3462-3469
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IF 2.596
Xiaoyan He,Huimin Han,Wenyu Shi,Jiandi Dong,Xiong Lu,Wu Yang,Xiaoquan Lu
It is urgent to develop a more simple and sensitive method to detect antibiotic residues considering the harm of antibiotic residues in food to the human body. Herein we designed a label-free electrochemical DNA biosensor for the sensitive detection of kanamycin (KAN) based on diblock DNA with a 15-mer of poly-cytosine (poly-C). The diblock DNA can be immobilized on graphene oxide (GO) due to strong physical adsorption between the 15-mer of poly-C and GO. The aptamer of KAN acted as the other block for rapidly binding the target. It can specifically capture the target, which leads to the change of electrochemical signal. Consequently, the DNA biosensor exhibited high sensitivity and specificity towards KAN, the linear range was from 0.05 pM to 100 nM with a detection limit of 0.0476 pM. The developed DNA biosensor was constructed easily and showed promising applications for the detection of antibiotic residues for food safety.
研究领域:
分析化学
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生物传感器
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食品科学
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电化学
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DOI
PubMed
高影响因子
Journal of the American Chemical Society
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2009
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IF 8.091
Yinghua Peng,Xiaohui Wang,Yi Xiao,Lingyan Feng,Chao Zhao,Jinsong Ren,Xiaogang Qu
The increasing worldwide demand for carbon nanotubes (CNTs) and increasing concern regarding how to safely develop and use CNTs are requiring a low-cost, simple, and highly sensitive CNT detection assay for toxicological evaluation and environmental monitoring. However, this goal is still far from being achieved. All the current CNT detection techniques are not applicable for automation and field analysis because they are dependent on highly expensive special instruments and complicated sample preparation. On the basis of the capability of single-walled carbon nanotubes (SWNTs) to specifically induce human telomeric i-motif formation, we design an electrochemical DNA (E-DNA) sensor that can distinguish single- and multiwalled carbon nanotubes both in buffer and in cell extracts. The E-DNA sensor can selectively detect SWNTs with a direct detection limit of 0.2 ppm and has been demonstrated in cancer cell extracts. To the best of our knowledge, this is the first demonstration of a biosensing technique that can distinguish different types of nanotubes. Our work will provide new insights into how to design a biosensor for detection of carbon nanotubes.
研究领域:
生物传感
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环境监测
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毒理学
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DOI
PubMed
高影响因子
Bioactive Materials
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2022
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IF 16.874
Yi Xiao,Haomin Yi,Jingzhi Zhu,Suhua Chen,Guofang Wang,Yilong Liao,Yuanyuan Lei,Liyin Chen,Xingcai Zhang,Fangfu Ye
Toxicity assessment is a major problem in pharmaceutical candidates and industry chemicals development. However, due to the lack of practical analytical methods for DNA adduct analysis, the safety evaluation of drug and industry chemicals was severely limited. Here, we develop a DNAzyme-based method to detect DNA adduct damage for toxicity assessment of drugs and chemicals. Among 18 structural variants of G4 DNAzyme, EA2 DNAzyme exhibits an obvious DNA damaging effect of styrene oxide (SO) due to its unstable structure. The covalent binding of SO to DNAzyme disrupts the Hoogsteen hydrogen bonding sites of G-plane guanines and affects the formation of the G4 quadruplex. DNA damage chemicals reduce the peroxidase activity of the G4 DNAzyme to monitor the DNA adduct damage by disrupting the structural integrity of the G4 DNAzyme. Our method for genotoxic assessment of pharmaceutical candidates and industrial chemicals can elucidate the complex chemical pathways leading to toxicity, predict toxic effects of chemicals, and evaluate possible risks to human health.
研究领域:
分子生物学
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毒理学
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药学
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化学工程
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产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2024
·
IF 5.6
Jiong Liang,Yunzhe Zhang,Zikun Li,Xin Lu,Congyan Qi,Qian Yang,Wei Zhang
Aflatoxin B1 is a potent carcinogen widely found in agricultural products and feed. Therefore, investigating the development of convenient, sensitive, and precise techniques for detecting AFB1 is critical. This work proposes a ratiometric electrochemical aptasensor based on DNAzyme-driven tripedal DNA walker for enzyme-free AFB1 detection. The design of multiple legs reduces the moving time on the electrode surface and enhances the value of signal response. In this approach, the tagging of ferrocene and methylene blue as signal switch molecules diminishes the impact of interferences and significantly enhances the reproducibility (RSD: 2.825%). Meanwhile, the tripedal DNA walker and DNAzyme dual signal amplification enabled the sensor to detect AFB1 traces in cereals. We achieved trace detection of AFB1 with a detection limit of 61 fg/mL and a wide linear range from 0.0001 to 10 ng/mL. Furthermore, the aptasensor success rate was comparable to that of HPLC-PMP. The results demonstrate enormous potential for detection. Furthermore, the method can detect various targets by altering the target aptamer , displaying attractive scalability. This suggests its tremendous prospective in detecting mycotoxins in food in the future.
研究领域:
食品安全检测
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生物传感技术
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分子诊断
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2018
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IF 8.097
Caixia Zeng,Na Lu,Yanli Wen,Gang Liu,Rui Zhang,Jiaxing Zhang,Fei Wang,Xiaoguo Liu,Qian Li,Zisheng Tang,Min Zhang
DNA treatment of metal nanoparticles provides a potent tool for tuning their native properties and constructing advanced materials. However, there have been limited studies on interactions between DNA and nanomaterial-based artificial enzymes (nanozymes) to influence their intrinsic peroxidase-like properties. Here, we present the utilization of DNA as a capping ligand to engineer various bio-nanointerfaces for high-precise and adjustable regulation of catalytic behaviors of nanozymes toward the oxidation of substrates. The treatment of stiff double-stranded DNA only induced a negligible enhancement of the catalytic activity of nanozymes, and both coil-like single-stranded DNA and hairpin DNA-capped nanoparticles produced a medium signal increase. Interestingly, hybridization chain reaction (HCR) product-treated nanoparticles showed the highest peroxidase-like activities among four DNA structures. Furthermore, significant parameters that influence HCR process and the modulation of catalysis, such as the concentration of the hairpin DNA, the ionic strength, and the amount of nanozyme, were also systematically investigated. On the basis of HCR amplification and iron oxide (Fe3O4) nanoparticles, we develop a simple, fast, label-free, and sensitive colorimetric strategy for sensing of a Yersinia pestis-relevant DNA sequence with a detection limit as low as 100 pM as well as single nucleotide polymorphism discrimination. These results highlight DNA engineering as a facile strategy to regulate the catalytic activities of nanozymes and understand the interactions between metallic nanoparticles and nucleic acids for biosensing applications.
研究领域:
生物化学
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纳米医学
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分析化学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2016
·Vol. 183
·期 11
·2965-2971
·
IF 4.831
Meng Yan,Hun Xu,Zhang Yue,Luo Xiliang
A toehold-aided DNA recycling amplification technology was developed based on the combination of toehold-aided DNA recycling and the hemin/G-quadruplex label. The dsDNA formed between aptamer and DNA1 was first immobilized on magnetic beads. On addition of target analyte (exemplified here for riboflavin), the aptamer-riboflavin complex is formed and DNA1 is released by the beads. After magnetic separation, the supernatant containing the released DNA1 is added to a solution containing the hairpin capture DNA on magnetic beads. DNA1 will hybridize with the hairpin capture DNA via toehold binding and branch migration. This process will open the hairpin structure, and an external toehold is formed in the newly formed dsDNA. On addition of reporter DNA containing the G-quadruplex, it will interact with the formed dsDNA via toehold binding and branch migration, resulting in the releasing of DNA1 and capturing of reporter DNA on the magnetic beads. The released DNA1 will bind to another hairpin capture DNA which can start another round of DNA1 recycling. Chemiluminescence (CL) is generated by the G-quadruplex-hemin-luminol CL reaction system. Under optimal conditions, the calibration plot is linear in the 0.1 to 700 nM riboflavin concentration range, with a 30 pM detection limit (at a signal-to-noise ratio of 3). The method was successfully applied to the quantitation of riboflavin in spiked urine samples.
Toehold-aided DNA recycling coupled with hemin G-quadruplex for target detection.
研究领域:
生物化学
·
分析化学
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分子生物学
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DOI
高影响因子
危险品化学品经营许可证（带存储）