DYES AND PIGMENTS
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2024
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IF 4.1
Xiaona Zhai,Song Yang,Shuai Wang,Pengcheng Wang,Debin Ji
DNAzymes have gained various applications in scientific fields, but their practical uses in everyday life are still being investigated. Hair dyeing, a popular cosmetic practice, still poses potential health risks, owing to the toxicity of commercial hair dyes. In this study, we assessed the feasibility of DNAzyme-catalyzed hair dyeing using natural pigments. We found that DNAzymes of peroxidase activity can expedite dopamine polymerization, resulting in the black coloration of human hair in around half an hour. This process can be achieved by using the milder Tris buffer to substitute the NH 4 OH with 0.1 mM H 2 O 2 . In addition, these DNAzymes can catalyze the polymerization of other natural pigments. Thus, our mild and rapid hair dyeing approach has the potential to substitute traditional harsh hair dyeing conditions and also provides new insights into the field of DNAzyme applications.
研究领域:
生物化学
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化妆品科学
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分子生物学
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高影响因子
MICROCHIMICA ACTA
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2026
·Vol. 193
·期 8
·574
·
IF 5.4
Lu Jiahui,Zhang Shijie,Li Runze,Deng Shuyuan,Wang Yiting,Tu Kang,Pan Leiqing,Yang Chuanyu
A novel “signal-on” fluorescence sensing strategy was carried out based on GR5 DNAzyme and covalent organic framework (COF) to detect lead ions (Pb 2+ ). The COF was synthesized at room temperature, with excellent structural stability and high fluorescence quenching efficiency, enabling low background signal and reliable detection. The GR5 DNAzyme that responds to Pb 2+ contains a large single-stranded loop structure, which can be adsorbed onto the COF, resulting in fluorescence quenching of the attached FAM fluorophore. In the presence of Pb 2+ , the GR5 DNAzmye is specifically activated and cleaved, releasing a short FAM-labeled fragment of 5 bases. Because this short fragment has weak affinity for the COF, it is released from the COF surface, leading to fluorescent recovery. The sensor based on COF/DNA enzyme shows high selectivity and sensitivity, with a detection limit of 0.067 nM. In addition, the method has been successfully applied to the detection of Pb 2+ in real water samples, with recoveries ranging from 89.58% to 112.70%. This work provides a simple and sensitive platform for Pb 2+ detection and further demonstrates the potential of COFs as high-efficient fluorescence quenchers in functional nucleic acid-based biosensing. Graphical
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高影响因子
SENSORS
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2013
·Vol. 13
·期 1
·1064-1075
·
IF 1.953
Zhenzhu Zheng,Jing Han,Wensheng Pang,Juan Hu
With an internal transcribed spacer of 18 S, 5.8 S and 26 S nuclear ribosomal DNA (nrDNA ITS) as DNA marker, we report a colorimetric approach for authentication ofPseudostellaria heterophylla(PH) and its counterfeit species based on the differentiation of the nrDNA ITS sequence. The assay possesses an unlabelled G-quadruplex DNAzyme molecular beacon (MB) probe, employing complementary sequence as biorecognition element and 1:1:1:1 split G-quadruplex halves as reporter. In the absence of target DNA (T-DNA), the probe can shape intermolecular G-quadruplex structures capable of binding hemin to form G-quadruplex-hemin DNAzyme and catalyze the oxidation of ABTS2−to blue-green ABTS•−by H2O2. In the presence of T-DNA, T-DNA can hybridize with the complementary sequence to form a duplex structure, hindering the formation of the G-quadruplex structure and resulting in the loss of the catalytic activity. Consequently, a UV-Vis absorption signal decrease is observed in the ABTS2−-H2O2system. The “turn-off” assay allows the detection of T-DNA from 1.0 × 10−9to 3.0 × 10−7mol·L−1(R2= 0.9906), with a low detection limit of 3.1 × 10−10mol·L−1. The present study provides a sensitive and selective method and may serve as a foundation of utilizing the DNAzyme MB sensor for identifying traditional Chinese medicines.Keywords:G-quadruplex DNAzyme;molecular beacon;colorimetric;Pseudostellaria heterophylla
研究领域:
生物化学
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分子生物学
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分析化学
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中药学
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2016
·
IF 4.758
Gonge Tang,Cailan Zhao,Jie Gao,Hongliang Tan
It is of great importance to simply and sensitively detect hydrogen sulfide (H 2 S) because of its role in various physiological processes as well as its inherent toxicity. In this work, a colorimetric method for H 2 S detection was developed by employing terbium-G-quadruplex-hemin (Tb/G4-hemin) DNAzyme as a peroxidase mimic, which can catalyze H 2 O 2 -mediated oxidation of 2,2′-azinobis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) to produce radical cation (ABTS + ). Compared with the G4-hemin DNAzyme promoted by K + and Na + , Tb/G4-hemin DNAzyme exhibits a higher catalytic activity. In the presence of Ag + , the peroxidase-like activity of Tb/G4-hemin DNAzyme can be inhibited significantly owing to the disruption of G-quadruplex structure. However, the addition of H 2 S can effectively suppress such negative behavior by competitive binding with Ag + , leading to the recovery of the peroxidase-like activity of Tb/G4-hemin DNAzyme, which can be reflected by an increase in absorbance signal of ABTS + . The absorbance of ABTS + was enhanced linearly with increasing H 2 S concentration from 20 nM to 2 μM. The detection limit for H 2 S is 13 nM, which is much lower than most of previous methods. Moreover, the proposed method possesses the features of simple preparation, easy reproducibility and good biocompatibility. Given the fine performances and striking properties, we believe that the Tb/G4-hemin DNAzyme would have a great promise for analytical applications.
研究领域:
分析化学
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生物化学
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分子生物学
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高影响因子
Science China-Chemistry
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2016
·Vol. 60
·期 2
·293-298
·
IF 2.429
Huang Yishun,Wu Xuemeng,Tian Tian,Zhu Zhi,Lin Hui,Yang Chaoyong
Lanthanide elements (Ln) play an important role in industry and agriculture. As a result of the increasing consumption of lanthanides, environmental emission of Ln has become detrimental to the health of flora and fauna. Current methods for trace lanthanides detection mainly rely on sophisticated instruments. In this article, a Ln 3+ dependent DNAzyme was incorporated into a hydrogel to generate Ln 3+ sensitive DNAzyme hydrogel for portable colorimetric detection. The enzyme strand and its substrate strand act as crosslinker and functional unit of the hydrogel with polyacrylamide chains as the scaffold and gold nanoparticles (AuNPs) as the indicator of hydrogel stability. Any ions in the Ln 3+ series can trigger the cleavage of substrate strand by activating the enzyme strand, thereby decreasing the crosslink ratio and leading to collapse of the hydrogel. The release of the encapsulated AuNPs turns the supernatant wine red. Using this colorimetric method, Ln 3+ can be detected with high sensitivity, with a limit of detection (LOD) of 20 nM for Ce 3+ . The hydrogel responds specifically to any Ln 3+ ion and works well with the spiked lake sample without the need of instruments and skilled operators. Our results suggest that the lanthanide responsive hydrogel can be used for portable and sensitive detection of Ln 3+ contamination in the field.
研究领域:
生物化学
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环境科学
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2015
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IF 4.097
Yanli Zhang,Shixiu Xiao,Haizhen Li,Hongjun Liu,Pengfei Pang,Hongbin Wang,Zhan Wu,Wenrong Yang
A novel strategy for selective and sensitive electrochemical lead ion (Pb 2+ ) biosensor was developed based on the single-stranded DNAzyme catalytic beacon. A DNAzyme that requires Pb 2+ for activation was selected and labeled with redox-active ferrocene (Fc) for signal transducer. The Fc-labeled single-stranded DNAzyme (Fc-ssDNAzyme) was self-assembled through S Au bonding on a gold electrode surface. In the presence of Pb 2+ , the ssDNAzyme was activated and catalyzed the hydrolytic cleavage of the substrate strand, resulting in the removal of the substrate strand along with the Fc from the Au electrode surface. The dissociation of Fc caused a decrease of electrochemical signal (“signal-off”). Under the optimal conditions, the electrochemical signal of Fc decreased directly with the increasing Pb 2+ concentration, exhibiting a linear response in the range of 0.5 nM to 5 μM with a detection limit of 0.25 nM. This strategy is simple, sensitive and selective with the minimal reagents and working steps, thereby holds great potential for Pb 2+ detection in real environmental sample analysis.
研究领域:
分析化学
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环境科学
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生物化学
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高影响因子
Biosensors-Basel
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2022
·Vol. 12
·期 5
·331
·
IF 5.743
Zhixue Yu,Hui Wang,Yiguang Zhao,Fan Zhang,Xiangfang Tang,Benhai Xiong
An electrochemical biosensor for detecting Ca2+concentration was proposed using glass carbon electrodes (GCEs) modified with nitrogen-doped graphene (NGR), gold nanoparticles (AuNPs) and DNAzyme. The resistance signal was amplified through two methods: electrochemical reduction of AuNPs on the NGR surface to increase the specific surface area of the electrode and strengthen the adsorption of DNAzyme; and increasement of the DNAzyme base sequence. The process of electrode modification was characterized by scanning electron microscopy, Raman spectroscopy, cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). Experimental parameters’ influence, such as the deposition time of gold nanoparticles and the detection time, were assessed by electrochemical methods. The linear ranges of the electrochemical biosensor were in the range from 5 × 10−6to 5 × 10−5and 5 × 10−5to 4 × 10−4M, with a detection limit of 3.8 × 10−6M. The concentration of Ca2+in the serum of dairy cows was determined by the biosensor with satisfactory results, which could be potentially used to diagnose subclinical hypocalcemia.Keywords:electrochemical biosensor;nitrogen-doped graphene;gold nanoparticles;DNAzyme;dairy cow;hypocalcemia
研究领域:
生物化学
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分析化学
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兽医学
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PubMed
高影响因子
ACS Nano
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2022
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IF 18.027
Yun Zhao,Ruomeng Li,Junlin Sun,Zhiqiao Zou,Fuan Wang,Xiaoqing Liu
High mortality and rapid development of metastasis requires the development of more effective antimetastasis strategies. However, conventional therapeutic methods, including surgery, radiation therapy, and chemotherapy, show less effectiveness in curbing the metastatic spread of cancer cells and the formation of metastases. A therapeutic platform, targeting the early stage of metastasis cascade, could effectively prevent metastasis dissemination. Herein, Fe/Mn-based metal–organic frameworks (FMM) were constructed for the delivery of a specific DNAzyme with high catalytic cleavage activity on the metastasis-involved Twist mRNA, thus efficiently inhibiting the invasion of cancer cells through DNAzyme-catalyzed gene silencing. Highly potent combined gene/chemodynamic therapy is achieved from the self-supplied DNAzyme cofactors and efficient glutathione depletion. Importantly, by virtue of the intrinsic photo-to-thermal conversion of the FMM nanocarriers, our combined therapeutic strategy could be further promoted under photothermal stimuli to speed up the Fenton reaction and to accelerate the release of the Twist DNAzyme with efficient gene therapy. Consequently, the effective elimination of tumors and the blockage of metastasis are simultaneously achieved under photothermal/magnetic resonance imaging guidance. This work aims at developing versatile theranostic agents to combat metastatic tumors.
研究领域:
癌症研究
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纳米医学
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基因治疗
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材料科学
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生物医学工程
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PubMed
高影响因子
ANALYTICAL LETTERS
·
2022
·
IF 2.267
Hongjing Sun,Zheng Liu,Zhihong Li,Xinyue Ma,Zixuan Duan,Chunyan Sun
A simple and label-free fluorescent sensor based upon a specific DNAzyme (8–17 DNAzyme) and thiazole orange (TO) for monitoring Pb2+ in water samples was described. The 8–17 DNAzyme was formed by an enzyme strand (17E) and a partially complementary substrate strand (17DS). Thiazole orange, the DNA intercalator, was nonfluorescent in aqueous solution while it intercalates into the 8–17 DNAzyme with a duplex structure resulting in strong fluorescence. In the presence of Pb2+, the 17E was activated and cleaved the 17DS into two at the ribonucleoside adenosine (rA) position, leading to the disruption of the duplex structure. This Pb2+-induced conformational change led to the quenching of the fluorescence of thiazole orange, thus forming the basis for the label-free biosensor. Under the optimized conditions, the developed protocol exhibited sensitive determination of Pb2+ with a detection limit of 0.73 nM and a linear range from 10 to 150 nM. The label-free fluorescent approach exhibited excellent selectivity, strong selectivity, and satisfactory analysis of a drinking water standard reference material and spiked river and tap water samples. Taking the advantage of the structure-switching 8–17 DNAzyme and the fluorescent intercalator of nucleic acids, this label-free sensor provides a new approach for routine screening of heavy metals based upon the DNAzyme.
研究领域:
分析化学
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环境科学
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生物化学
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高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Yujie Sun,Qi Dong,Huan Yang,Weiling Song,Hong Zhou
Telomerase activity detection has attracted much attention concerning its importance for early cancer diagnosis. Here, we established a ratiometric electrochemical biosensor for telomerase detection based on CuS quantum dots (CuS QDs) dependent DNAzyme-regulated dual signals. The telomerase substrate probe was used as the linker to combine the DNA fabricated magnetic beads and CuS QDs. In this way, telomerase extended the substrate probe with repeated sequence to from hairpin structure, releasing CuS QDs as an input to DNAzyme modified electrode . DNAzyme was cleaved with high current of ferrocene (Fc) and low current of methylene blue (MB). On the basis of the obtained ratiometric signals, telomerase activity detection was achieved in the range of 1.0 × 10 −12 -1.0 × 10 −6 IU/L, with the limit of detection down to 2.75 × 10 −14 IU/L. Moreover, telomerase activity from HeLa extracts was also tested to verify the clinical application.
研究领域:
生物化学
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分析化学
·
癌症研究
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PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Jiabin Yang,Min Wang
Background Abnormal expression of microRNA (miRNA) is closely associated with the occurrence and development of cancer and various other diseases. Therefore, obtaining quantitative information on miRNA expression, especially detecting miRNA expression levels in living cells, is of great significance for studying related physiological and pathological processes as well as diagnosing diseases. RNA-cleaving DNAzyme (RCD)-based DNA walkers have shown great potential in the detection of miRNA. However, their application is hindered by low intracellular delivery efficiency, limited catalytic efficiency, and the requirement for additional supply of cofactors. Results In this study, we developed a novel self-feedback dual-walker (SFDW) nanosystem, loaded onto urchin-like nano-MnO 2 (UNM), for intracellular trace-level miR-21 imaging. The SFDW nanosystem incorporates a hairpin-structured capture probe and two mutually activating DNA walkers. During the detection process, the capture probe recognizes and binds to miR-21 in the matrix, opens the hairpin structure, and subsequently activates the DNA walkers. The cleavage product of each DNA walker in the nanosystem serves as the activator for the other, thereby establishing a biocircuit (self-feedback circuit) with cyclic amplification effect between the two. This mechanism significantly enhances the signal amplification capability. The UNM carrier not only promotes cellular uptake for the SFDW nanosystem, but supplies endogenous Mn 2+ cofactors via intracellular glutathione-triggered degradation as well. The SFDW nanosystem achieved a low detection limit of 2.8 pM, and successfully enabled miRNA visualization in living cells. Significance The self-feedback circuit improved detection sensitivity and reduced the detection limit, while the introduction of UNM enhanced delivery efficiency and provided the necessary cofactors. This proposed strategy can address the limitations of RCD-based DNA walkers in intracellular miRNA detection and holds great potential for clinical diagnosis and early-stage disease screening.
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高影响因子
BIOSENSORS & BIOELECTRONICS
·
2025
·
IF 10.5
Shixin Cai,Rongfeng Cai,Sanxia Wang,Xin Chen,Lele Zhang,Yunpeng Zhao,Xiaoli Wang,Yuting Zhang,Nandi Zhou
Circulating tumor cells (CTCs) are closely associated with cancer progression and metastasis, making the development of precise CTCs enumeration technology crucial for real-time cancer monitoring and post-operative treatment. Researches indicated that miRNA-21 exhibits abnormally high expression in CTCs and can thus serve as a biomarker for CTCs profiling. Herein, an innovative dual-responsive detection platform was developed by integrating miRNA-21/pH-activated ZnO@Au NPs-hairpin DNA (HP) nanoprobes with microfluidic single-cell droplet technology for CTCs identification. In this platform, ZnO@Au NPs-HP nanoprobes and CTCs are co-encapsulated into single-cell droplets, after which the nanoprobes enter CTCs. Leveraging the characteristic intracellular high miRNA-21 level and acidic microenvironment of CTCs, Zn-DNAzyme contained in HP can be activated to cleave its substrate chain and the fluorescence is thus restored. This strategy effectively avoids the interference from free miRNA-21 in blood and enhances detection specificity. Subsequent droplet fluorescence detection enables the accurate CTCs quantification. The platform enables robust detection and precise isolation of CTCs in blood samples from three distinct cancer cell types HCT116, HepG2 and HeLa, and achieves remarkably high sensitivity with successful recovery of 5 CTCs/mL in spiked human blood samples. Furthermore, the platform has been successfully applied for the accurate counting of CTCs in whole blood samples from patients with three types of cancers, i.e. colorectal cancer, hepatocellular carcinoma, and cervical cancer. Therefore, the established system exhibits superior detection performance and significant clinical potential, providing a robust tool for liquid biopsy and personalized cancer management.
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高影响因子
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