PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2022
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IF 4.966
Shiran Huang,Ming Huang,Sinuo Tian,Zhiyuan Meng,Sen Yan,Miaomiao Teng,Zhiqiang Zhou,Jinling Diao,Wentao Zhu
Imazalil (IMZ) is a highly effective fungicide employed in crop production. It has been consistently detected in aquatic environments . The main environmental metabolite of IMZ is imazalil-M (IMZ-M). Limited studies have focused on the toxicity of IMZ and IMZ-M in aquatic organisms . This study systematically evaluated the developmental toxicity of IMZ and IMZ-M on zebrafish ( Danio rerio ) embryos and explored the potential mechanisms involved. The results showed that IMZ and IMZ-M caused developmental toxicity, characterized by decreased heart rate, hatching inhibition, and pericardial cyst in zebrafish embryos. Subsequently, acridine orange (AO) staining revealed cell apoptosis in the area around the heart regions of zebrafish larvae. Besides, the expression levels of apoptosis-related genes also varied significantly. Furthermore, 1 H NMR-based metabolomics analysis showed that IMZ and IMZ-M exposure could induce metabolic profiles disorder in zebrafish larvae. Importantly, zebrafish exposure to IMZ and IMZ-M significantly affected the metabolism of branched - chain amino acids, energy, and ketone bodies , which are related to cell apoptosis. Overall, the toxicity of IMZ and IMZ-M in zebrafish embryos and larvae was characterized, suggesting a theoretical basis for the potential environmental risks of IMZ and its metabolite IMZ-M on non-target organisms.
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ENVIRONMENTAL POLLUTION
·
2019
·
IF 5.714
Yan Zhang,Jianfeng Feng,Yongfei Gao,Xinyong Liu,Liang Qu,Lin Zhu
Accurately predicting the accumulation and toxicity of metals in organisms is a challenging work in ecotoxicology . Here, we developed and validated a physiologically based toxicokinetic and toxicodynamic (PBTK-TD) model for adult zebrafish exposed to Cd and Pb. The model included the gill, liver, intestine, gonad, carcass, and brain, which were linked by blood circulation in the PBTK process and by dynamic relationships between the target organ concentrations and mortality in the TD process. Results showed that the PBTK sub-model can accurately describe and predict the uptake, distribution and disposition kinetics of Cd and Pb in zebrafish. The exchange rates and the accumulation of the metals in the organs were significantly different. For Cd, the highest exchange rate was between blood and liver, and the greatest accumulation of Cd occurred in the liver. For Pb, the greatest accumulation occurred in the gill. The TD sub-model further indicated that metal concentrations in the gill may effectively act as more suitable indicator of Cd and Pb toxic effect than whole body or other organs. The proposed PBTK-TD model is helpful to understanding the fundamental processes by which zebrafish regulate the uptake and disposition of metal and to quantitatively predicting metal toxicity.
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COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
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2025
·
IF 4.3
Qiuyu Liu,Chuxiao Hu,Xinqin Li,Qiulan Liu,Wenjie Xia,Yuan Ren
The frequent detection of psychoactive drugs in aquatic environments has caused various toxic effects on aquatic organisms, highlighting the urgent need to explore remediation methods and mechanisms. Against the backdrop of toxicity induced by the typical benzodiazepine (BZD) flunitrazepam (FLZ) in zebrafish, this study evaluates the mitigating effects of vitamin C (VC) on FLZ-induced embryonic developmental toxicity, larval behavioral anomalies, apoptosis, oxidative stress, and mitochondrial dysfunction at environmentally relevant concentrations through reactive oxygen species (ROS)-mediated pathways. Furthermore, molecular dynamics simulations were utilized to decipher the mechanism underlying ROS inhibition. Results demonstrated that co-exposure to 0.5 μg/L VC with FLZ (0.05 μg/L and 0.2 μg/L) significantly elevated the hatching rate of zebrafish embryos at 72 hpf and decreased the larval malformation rate at 96 hpf. In terms of physiological and biochemical indicators, VC significantly inhibited the FLZ-induced increase in ROS and 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels. VC also upregulated the activity of mitochondrial uncoupling protein 2 (UCP2), a key regulator of ROS production. Molecular docking and dynamics simulations revealed that VC competitively binds to the LYS 38 and LYS 240 sites of UCP2, destabilizing FLZ-UCP2 interactions via steric hindrance and hydrogen bond competition. With the restoration of UCP2 activity, its proton leak function was enhanced, suppressing excessive ROS generation. Consequently, uqcr2b , cox4i1l , and atp5g3b were normalized, restoring ATP synthesis capacity and significantly alleviating FLZ-induced mitochondrial dysfunction. This study elucidates the mechanism by which VC counteracts ROS-mediated FLZ toxicity, providing critical insights for assessing environmental risks and formulating protective strategies against pollutants.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2025
·
IF 6.1
Yilong Zou,Yu Zhou,Xianglian Wang,Guiqing Gao,Gang Chen,Zhanmeng Liu,Mi Deng,Xueping Huang,Yongming Wu
Concerns regarding the possible health and environmental hazards of sodium p -perfluorous nonenoxybenzene sulfonate (OBS), a new substitute for perfluorooctane sulfonate, have been raised by its detection in a range of environmental matrices, animals, and people. Despite its widespread presence, research on the toxic effects and toxicokinetics of OBS remains limited. Therefore, in this study, we aimed to provide the first detailed investigation into how tissue distribution, bioaccumulation, and OBS exposure cause oxidative damage in adult zebrafish. Zebrafish were exposed to OBS at doses of 0, 10, and 100 μg/L for 28 days, following a 28-day depuration phase. Our findings indicate that OBS accumulated rapidly in zebrafish, with log kinetic bioconcentration factor (logBCF k ) values ranging from 2.48 to 2.70 and slow elimination rates ( k e = 0.0490–0.0643 d −1 ). The blood, liver, and muscle were responsible for over 50 % of the total body burden of OBS, with tissue concentrations decreasing in the following order: blood > liver > intestine > gills > gonad > brain > muscle, suggesting a tissue-specific accumulation pattern. Molecular docking analysis showed that OBS exhibited binding energies of −2.71 and −3.82 kcal/mol with organic anion transporter 1 and human serum albumin, respectively. Additionally, chronic exposure to OBS resulted in significant liver damage in zebrafish, including ballooning, cytoplasmic vacuolisation, and oxidative stress. This study provides valuable experimental data on the toxicokinetics and in vivo toxicology of OBS in zebrafish, offering important insights for environmental risk assessments.
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FISH & SHELLFISH IMMUNOLOGY
·
2020
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IF 0
Shaolin Xie,Aiguo Zhou,Nan Xu,Yongyong Feng,Zhengkun Pan,Muhammad Junaid,Jun Wang,Jixing Zou
Benzo[a]pyrene (BaP) is one of the most well studied carcinogenic polycyclic aromatic hydrocarbons (PAHs) that has been associated with a wide range of toxic effects in aquatic organisms . In the present study, the mosquitofish and zebrafish were exposed to BaP (100 μg/L) for 15 days. We analyzed the intestinal microbial community of mosquitofish and zebrafish using 16S rRNA gene amplicon sequencing and also performed transcriptional profiling of the inflammation pathway related genes in the intestinal tissues. Our results showed that BaP exposure induced similar changes to the composition of microbial community in mosquitofish and zebrafish. At the phylum level, the abundance of Proteobacteria decreased while the abundance of Firmicutes increased following BaP exposure. At the genus level, a common pathogenic genus staphylococcus significantly increased in the BaP treatment groups, compared to the control (DMSO, ~0.001% v/v). In addition, it was observed that BaP significantly increased the mRNA level of il1β in both mosquitofish and zebrafish. The transcript levels of il6 , il8 , il10 and ifnphi1 were significantly increased in zebrafish, however not in mosquitofish, following Bap exposure. Our findings suggest that BaP could induce microbiota dysbiosis and inflammation in the intestine of mosquitofish and zebrafish.
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Journal of Hazardous Materials Advances
·
2026
·
IF 9.1
Xin-Ru Zhang,Yue-Hua Chen,Gui-Zhong Xin,Cheng Qian,Mu Zhang,Xiao-Wei Zhang,Zi-Xuan Ding,Fang-Tong Liu,Hui Zhang,Rui-Bo Sun,Tian-Qi Zhang,Hong-Juan Hou,Kang Ting-Guo,Hui-Peng Song
The aquatic toxicity mechanism of methyl eugenol (ME), an emerging insect sex pheromone-based pesticide, remains unclear. To address this issue, we established a strategy termed "1N (network toxicology) + 2T (toxicity assessment, transcriptomics) + 3M (metabolomics, molecular docking, mechanism verification)" to systematically investigate its toxicity, focusing on ferroptosis as a potential core mechanism. Toxicity assessment determined the 96-hour LC₅₀ of ME in zebrafish to be 2.536 mg/L and it caused marked histopathological damage in liver and gill tissues. Furthermore, integrated transcriptomic and metabolomic analysis revealed the co-enrichment of dysregulated genes and altered metabolites in ferroptosis-related pathways. Subsequently, network toxicology identified CDK1, TP53, CHEK2, and CTNNB1 as key ferroptosis-related targets of ME, and molecular docking confirmed their stable binding via hydrogen bonds with lysine residues. For mechanism verification, the ferroptosis inhibitor ferrostatin-1 (Fer-1) significantly reduced ME-induced mortality, alleviated tissue damage, and mitigated oxidative stress and lipid peroxidation. These findings demonstrated that ferroptosis was the primary toxicity mechanism of ME in zebrafish. Our study provides scientific evidence for the ecological risk assessment of ME and presents the "1N+2T+3M" strategy as an applicable framework for evaluating other pesticides.
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SCIENCE OF THE TOTAL ENVIRONMENT
·
2018
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IF 4.61
Jianfeng Feng,Yongfei Gao,Min Chen,Xin Xu,Mengdi Huang,Tong Yang,Na Chen,Lin Zhu
Protons and cations may affect metal accumulation in aquatic organisms and further influence metal toxicity . The effects of K + , Na + , Ca 2+ , Mg 2+ , and H + on the accumulation and toxicity of Cd and Pb in zebrafish larvae after 24 h exposure were examined. We found that Na + , Ca 2+ , Mg 2+ , and H + exerted significant effects on both the accumulation and toxicity of Cd, and Ca 2+ , Mg 2+ , and H + also affected both the accumulation and toxicity of Pb significantly. Subsequently, stability constants for the binding of Pb 2+ , Cd 2+ , K + , Ca 2+ , Mg 2+ , Na + , and H + to biotic ligand were estimated with the Langmuir model and biotic ligand model (BLM). Using the BLM-estimated binding constants calculated with toxicity data, a refined toxicokinetic-toxicodynamic (TK-TD) model considering cation competition effects was used to predict Cd and Pb accumulation and survival rates in zebrafish larvae with varying cation concentrations. Results showed that the developed TK-TD model could successfully predict Cd and Pb toxicity to zebrafish larvae as a function of major competitive cations. The TK-TD model incorporated cation competition effects is a promising tool to quantify and assess the metal risk in natural water.
研究领域:
生态毒理学
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环境化学
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水生毒理学
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模型与风险评价
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CHEMOSPHERE
·
2023
·
IF 8.8
Fang Jiao,Yang Zhao,Samwel Mchele Limbu,Lingfu Kong,Daitao Zhang,Xianghe Liu,Sha Yang,Wenjun Gui,Hua Rong
Cyhexatin (CYT), an organotin acaricide , is extensively utilized in developing countries to mitigate plant diseases caused by mites and minimize agricultural crop losses. However, the comprehensive mechanisms underlying the developmental stage of non-target organisms remain largely unexplored. In this study, zebrafish embryos were firstly exposed to CYT (0.06, 0.12, and 0.20 ng/mL, referred to as CYTL, CYTM, and CYTH, respectively) from 2 hpf (hours post fertilization) to 30 dpf (days post fertilization). No developmental toxicity was observed in the CYTL and CYTM groups, except for induced deformed phenotypes in the CYTM group at 120 hpf. However, exposure to CYTH resulted in significant reductions in spontaneous movement (24 hpf), heart rate (48 hpf), hatching rate (48 and 72 hpf), body weight (30 dpf), whole body length (30 dpf), and locomotion (30 dpf). Additionally, CYTH exposure induced morphological malformations, including spinal curvature, pericardial edema, and tail curvature in zebrafish larvae. Moreover, CYTH treatment induced apoptosis, increased reactive oxygen species (ROS) production, and resulted in significant reductions in free T3, cholesterol, estradiol, and testosterone levels in zebrafish larvae, while free T4 levels were increased. RNA-Seq analysis indicated that CYTH exposure led to significant alterations in the genome-wide gene expression profiles of zebrafish, particularly in the thyroid hormone and steroid biosynthesis signaling pathways, indicating endocrine disruption. Furthermore, CYTH exposure induced global DNA hypomethylation, reduced S-adenosylmethionine (SAM) levels and the SAM/S-adenosylhomocysteine (SAH) ratio, elevated SAH levels, and suppressed the mRNA expression of DNA methyltransferases (DNMTs) while also downregulating DNMT1 at both the gene and protein levels in zebrafish larvae. Overall, this study partially elucidated the developmental toxicity and endocrine disruption caused by CYT in zebrafish, providing evidence of the environmental hazards associated with this acaricide.
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ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
·
2019
·Vol. 38
·期 10
·2169-2177
·
IF 3.421
An He,Xinyong Liu,Liang Qu,Yongfei Gao,Jianfeng Feng,Lin Zhu
We exposed zebrafish ( Danio rerio ) to different concentrations of lead and cadmium, and monitored them for survival at 24, 48, 72, and 96 h. Metal toxicity was predicted and compared using the dose–response and general threshold survival models in terms of required data sets, fit performance, and applicability. Environ Toxicol Chem 2019;38:2169–2177. © 2019 SETAC.
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JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Mingyu Sang,Shuyu Liu,Haohao Yan,Bing Zhang,Siyuan Chen,Bowen Wu,Tian Ma,Hanyi Jiang,Peichao Zhao,Guanjun Sun,Xinyan Gao,Hailian Zang,Yi Cheng,Chunyan Li
Triclocarban (TCC), an emerging organic contaminant, poses a potential threat to human health with long-term exposure. Here, Rhodococcus rhodochrous BX2 and Pseudomonas sp. LY-1 were utilized to degrade TCC at environmental related concentrations for enhancing TCC biodegradation and investigating whether the toxicity of intermediate metabolites is lower than that of the parent compound. The results demonstrated that the bacterial consortium could degrade TCC by 82.0% within 7 days. The calculated 96 h LC 50 for TCC, as well as its main degradation product 3,4-Dichloroaniline (DCA) were 0.134 mg/L and 1.318 mg/L respectively. Biodegradation also alleviated histopathological lesions induced by TCC in zebrafish liver and gut tissues. Liver transcriptome analysis revealed that biodegradation weakened differential expression of genes involved in disrupted immune regulation and lipid metabolism caused by TCC, verified through RT-qPCR analysis and measurement of related enzyme activities and protein contents. 16 S rRNA sequencing indicated that exposure to TCC led to gut microbial dysbiosis , which was efficiently improved through TCC biodegradation, resulting in decreased relative abundances of major pathogens. Overall, this study evaluated potential environmental risks associated with biodegradation of TCC and explored possible biodetoxification mechanisms, providing a theoretical foundation for efficient and harmless bioremediation of environmental pollutants.
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PLoS One
·
2026
·Vol. 21
·期 5
·e0347661
·
IF 2.8
Lan-Chen Zhang,Yan Li,Ming-Fei Wu,Jin-Qiu Jia,Pan-Pan Jia
Zebrafish ( Danio rerio ), Marine medaka ( Oryzias melastigma ), and freshwater medaka ( Oryzias latipes ) are three major animal species in evaluation of environmental pollutant toxicity and building of human disease models with conserved physiological and molecular pathways. Bacterial strains from gut microbiota should firstly be isolated for their functions’ exploration. In this study, we optimized a culture-based workflow under aerobic and anaerobic conditions to recover intestinal bacteria from adult zebrafish (ZF), marine medaka (MM), and freshwater medaka (OL) using both dissection-based and in vivo sampling. The isolates were then identified by Gram staining, 16S rRNA gene sequencing, phylogenetic analysis, and API 20E-based biochemical characterization. The culturable bacteria isolated from gut microbiota were identified belonged to 14 genera in ZF, 12 genera in MM, and 18 genera in OL. Comparative analysis showed clear differences in the composition of cultured gut bacteria among the three fish species. Overall, the library and the differences of gut bacteria in three key fish models provided the potential applications of bacterial strains as the probiotics, to against the fish pathogens and to increase the pollutant toxicity-resistant. Moreover, the bacterial library will support the deep researches combined with germ-free (GF) animals to clarify the relationships of intestinal microbiota to host health.
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Toxics
·
2026
·Vol. 14
·期 5
·388
·
IF 4.9
Xiaoli Wu,Xinyue Zhang,Zengliang Ruan
Trans , trans -2,4-decadienal ( tt -DDE), the primary aldehyde component found in cooking oil fumes, is a prevalent environmental pollutant. However, its potential adverse effects on ocular development remain largely unexplored. This study evaluated its toxicity on ocular development and angiogenesis in zebrafish larvae, as well as on human retinal vascular endothelial cells (HRECs). Zebrafish ( Danio rerio ) larvae at 48 h post-fertilization were microinjected intraocularly with various doses of tt -DDE (65.87–521.3 mM) for 24 h. We observed dose-dependent impairments in ocular development following tt -DDE exposure. It significantly reduced eye size and inhibited the intraocular vascular area at concentrations of 128.9 mM and above. Histopathological analysis revealed retinal structural disorganization, eye shrinkage, and a clear dose-dependent increase in acridine orange (AO) fluorescence intensity. Apoptosis assays confirmed a significant escalation in ocular cell death at higher exposure doses. Additionally, our results demonstrated that tt -DDE (5–100 μM) significantly reduced the viability of HRECs in vitro. These findings suggest that early-life exposure to tt -DDE impairs ocular development in zebrafish by inducing histopathological damage, inhibiting angiogenesis, and promoting apoptosis, and also exerts direct cytotoxicity to human retinal cells. This study underscores the potential risk of tt -DDE exposure as an environmental factor contributing to ocular developmental toxicity.
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