Chemosensors
·
2024
·Vol. 12
·期 10
·214
·
IF 3.7
Yun Hui,Weijun Kong,Weiliang Shu,Zhiting Peng,Fengshan Shen,Mingyang Jiang,Zhen Xu,Tianzhun Wu,Wenhua Zhou,Xue-Feng Yu
The measurement of cardiac troponin I (cTnI) is of vital importance for the early diagnosis of acute myocardial infarction. In this study, an enhanced electrochemiluminescent immunoassay for the highly sensitive and precise determination of cTnI was reported. A biomimetic chip with nepenthes peristome surface microstructures to achieve single-layer microbead arrays and integrated microelectrode arrays (MEAs) for ECL detection was microfabricated. Ru@SiO2nanoparticles were prepared as signal amplificators labeling immunomagnetic beads. Dendrimer-encapsulated platinum nanoparticles (Pt DENs) were electrochemically modified on ITO MEAs. The resulting Pt DEN-modified ITO MEAs preserved good optical transparency and exhibited an approximately 20-fold ECL signal amplification compared to that obtained from bare ITO. The method made full use of the biomimetic chip with Pt DENs to develop single-layer immunomagnetic bead arrays with increasingly catalyzed electrochemical oxidation of the [Ru(bpy)3]2+–TPA system. Consequently, a limit of detection calculated as 0.38 pg/mL (S/N = 3) was obtained with excellent selectivity, demonstrating significant potential for the detection of cTnI in clinical diagnostics.
研究领域:
生物医学工程
·
分析化学
·
纳米技术
·
心脏病学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Xin Xiong,Yao Zhang,YinFang Wang,HaiFeng Sha,Nengqin Jia
By virtue of the metal organic framework UiO-66-NH 2, an ingenious electrochemiluminescence immunosensor was successfully constructed for subtle exploration of the breast cancer biomarker CA 15-3. In this protocol, Ru(bpy) 3 Cl 2 ·6H 2 O (Ru(bpy) 3 2+ ), as a conventional luminescent reagent, provides a stable and strong luminescent signal. The UiO-66-NH 2 serves as a carrier to provide a large specific surface area for enhancing the electrochemical luminescence signal response of Ru(bpy) 3 2+ . UiO-66-NH 2 , Ru(bpy) 3 2+ are fixed together with Nafion membrane, and CA 15-3 antibody is covalently conjugated onto the membrane through the amide reaction. Ru(bpy) 3 2+ @UiO-66-NH 2 is used as the detection system. The experimental results reveal that the ECL signal decreased obviously after the CA 15-3 antigen molecule is captured on the immunosensor, with the capture of manifesting the principle of electrochemical luminescence quenching detection. Under optimal conditions, the sensor is sensitive and selective for detecting CA 15-3 with the detection range from 5 × 10 −4 to 5 × 10 2 U mL -1 and the low LOD (1.7705 × 10 -5 U mL -1 ). Furthermore, multiple experiments elucidate that the as-prepared sensor could be used for susceptibly detect CA 15-3 in actual samples, which has underlying application prospects in biological analysis.
研究领域:
分析化学
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生物化学
·
癌症研究
·
材料科学
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产品关联
DOI
高影响因子
TALANTA
·
2018
·
IF 4.244
Qiao Liu,Xing-Li Xie,Chang-Jie Mao,Jing-Shuai Chen,He-Lin Niu,Ji-Ming Song
A highly selective DNA-based electrochemiluminescence (ECL) based biosensor is described for the detection of human IgG. It is exploiting the effect of steric hindrance that affects the strength of the ECL signal in the presence of IgG. Digoxin-linked signaling DNA was specifically bound to IgG, and this causes steric hindrance which limits the ability of DNA to hybridize with capturing DNA attached to a gold electrode. Europium (II) doped CdSe quantum dots were covalently linked to the DNA in order to generate the ECL signal. Using this steric hindrance hybridization method, the ECL signal of the biosensor were proportional to the concentration of IgG with a wide linear range and a 14 pM detection limit. Conceivably, the method can be expanded to the detection of a wide range of proteins for which homologous recognition elements are available.
研究领域:
生物化学
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分析化学
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生物传感器
·
免疫学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 16
·13217-13223
·
IF 3.289
Xiaomin Fu,Zhengcong Gu,Qiyi Lu,Jiayao Liao,Shihong Chen
C60/graphite-like carbon nitride nanosheet (C60/g-C3N4 NS) hybrids were prepared by a simple π–π interaction, and used to construct a solid state electrochemiluminescence (ECL) sensor. Based on the fact that melamine could efficiently quench the ECL intensity of g-C3N4 NS, such a sensor achieved detection of melamine. The possible quenching mechanisms were discussed. Due to the combination of the excellent ECL behavior of g-C3N4 NS with the outstanding electronic conductivity of C60, the prepared sensor exhibited a response for melamine with a wide linear range of 5.0 × 10−13 to 2.7 × 10−11 M and 2.7 × 10−11 to 1.9 × 10−8 M. The detection limit was 1.3 × 10−13 M. In this work, the linear range of melamine was improved by six orders of magnitude and the detection limit as low as sub-picomolar. Such a construction strategy provided a simple, sensitive, low-cost and green detection method for melamine.
研究领域:
分析化学
·
材料科学
·
食品安全检测
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Jintao Cai,Tao Chen,Yuanhong Xu,Shuang Wei,Weiguo Huang,Rui Liu,Jingquan Liu
Molecularly imprinted technique (MIT) has proven to be a significant tool in the analyzing area in virtue of its obvious advantages such as specific recognition, favorable stability to high temperature and higher sensitivity. Electrochemiluminescence (ECL) technology has also been receiving enormous attention as a powerful tool in sensing fields. However, sensors based on the combination of MIT and ECL technologies have seldom been reported yet. Herein, we find that Ru(bpy) 3 2+ cannot only work as an efficient catalyst for photo-induced electron transfer–reversible addition–fragmentation chain transfer (PET-RAFT) polymerization, but also as a sensing probe for ECL sensor. Based on this, we successfully construct ECL sensors via the combination of MIT and ECL techniques. In details, poly(methacrylic acid) (PMAA) and cross-linked PMAA were synthesized first via a well-controlled PET-RAFT polymerization using Ru(bpy) 3 2+ as catalyst under illumination of visible light with a wavelength of 460 nm, as confirmed by 1 H NMR and gel permeation chromatography (GPC). Then, negatively-charged Au nanoparticles (AuNPs) with average sizes of 20 nm were prepared and modified with Ru(bpy) 3 2+ via electrostatic incorporation. MIPs were prepared on the surface of AuNPs using melamine (MEL) as the template via PET-RAFT controlled cross-linking polymerization. The MIPs modified AuNPs (AuNPs-MIPs) were then fixed on the surface of working electrode with Nafion to achieve a solid-state ECL sensing platform employing Ru(bpy) 3 2+ as the ECL probes. The as-prepared sensor showed a wide detection range of 5.0 × 10 –13 − 5.0 × 10 −6 mol/L and a low detection limit of 1.0 × 10 –13 mol/L (S/N ≥ 3) was reached in the detection of MEL. Moreover, further tests for analyzing MEL structural analogues proved that the constructed ECL sensing platform could be utilized to detect various substances via specific recognitions.
研究领域:
分析化学
·
传感技术
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 5
·1-13
·
IF 5.3
Zhang Jing,Yin Zhaojiang,Xiong Wei,Fan QiQi,Yu Fengyao,Liao Fusheng,Fan Hao,Qu Fei,Yu Qiangqiang
A novel, low-toxicity Ru-Ni-BPY metal–organic framework (MOF) nanomaterial was developed and a simple and sensitive dual-mode "on–off" ferric ions (Fe 3+ ) sensing platform based on this material constructed. The dual-mode detection method enabled cross-validation of the electrochemiluminescence (ECL) and fluorescent (FL) signals, enhancing accuracy and effectively minimizing errors. In linear ranges of 0.005–10 μM and 0.078–10 μM, Fe 3+ can dramatically quench the ECL and FL signals of the Ru-Ni-BPY MOF, with quenching rates of 91.42% and 91.31%, respectively, and detection limits of 0.1 nM and 9.3 nM, respectively. This ECL method not only achieved efficient detection of Fe 3+ in rat brain microdialysis fluid but also successfully enabled real-time FL detection of Fe 3+ in nude mice. Therefore, this platform has great potential for applications in environmental protection, life sciences, and early disease diagnosis. Graphical abstract
研究领域:
分析化学
·
材料科学
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生物化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Chemical Science
·
2024
·
IF 7.6
Yuheng Fu,Bingbing Xie,Miaoxia Liu,Shaojuan Hou,Qunyan Zhu,Alexander Kuhn,Lin Zhang,Wensheng Yang,Neso Sojic
Electrochemiluminescence (ECL) has emerged as a valuable tool for understanding multiphasic and compartmentalized systems, which have crucial wide-ranging applications across diverse fields. However, ECL reactions are limited to the vicinity of the electrode surface due to spatial constraints of electron transfer and the short lifetime of radical species, making ECL emission in bulk multiphasic solution challenging. To address this limitation, we propose a novel bipolar electrochemistry (BPE) approach for wireless dual-color ECL emission at the water/oil (w/o) interface. Firstly, amphiphilic glass carbon (GC) microbeads with distinct hydrophilic and hydrophobic regions are prepared by bipolar electrografting of hydrophobic trifluoromethyl diazonium salt, then the resulting Janus beads are positioned at the w/o interface. Subsequently, two model ECL systems containing luminol and H2O2 in the aqueous phase, and [Ru(bpy)3]²⁺ and benzoyl peroxide (BPO) in the organic phase, are selected based on their solubility to confine light-emitting reactions to their respective phases. Upon application of an electric field perpendicular to the interface, the Janus microbeads get polarized, triggering simultaneous oxidative blue ECL (425 nm) and reductive red ECL (620 nm) in the aqueous and organic phases, respectively. Taking advantage of ECL imaging, the potential gradient distribution on the GC microbead at the w/o interface is revealed, indicating a "pseudo-closed" bipolar system due to limited ion transfer between phases. We also investigate the effect of changing the electric field direction parallel to the interface, which alters the ECL emission area from a hemisphere to a quarter of the microbead's surface. This bipolar ECL approach at the w/o interface not only offers opportunities for imaging the aqueous phase and organic phase simultaneously, but also enables ECL imaging and light generation in the bulk solution, thus overcoming the usual spatial limitation requiring proximity to the electrode surface.
研究领域:
分析化学
·
物理化学
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材料科学
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2012
·
IF 5.602
Yuqi Yu,Qiao Cao,Ming Zhou,Hua Cui
We report a novel homogeneous label-free aptasensor for 2,4,6-trinitrotoluene (TNT) detection based on an assembly strategy of electrochemiluminescent graphene oxide (GO) with gold nanoparticles (AuNPs) and aptamer . In this sensing strategy, the anti-TNT aptamer was first assembled with AuNPs to form aptamer–AuNPs. Then ruthenium(II) complex functionalized GO (denoted as Ru–GO) was assembled with aptamer–AuNPs by electrostatic interaction . AuNPs could directly quench the electrochemiluminescence (ECL) emission of ruthenium(II) complex on the surface of Ru–GO due to the energy transfer from luminophore to the AuNPs. Weak ECL signal of the assembly was obtained. In the presence of target molecule TNT, the aptamer–AuNPs would aggregate partly due to the aptamer–target interaction and reduce quenching effect, leading to ECL signal restoration and strong ECL signal was obtained. TNT in a range of 0.01–100 ng mL −1 could be detected by use of the ECL intensity discrepancy with a low detection limit of 3.6 pg mL −1 . The aptasensor also showed high selectivity towards TNT against 2,4-dinitrotoluene, p-nitrotoluene and nitrobenzene . The present aptasensor has been successfully applied to the detection of TNT in real water samples. Compared with previous reported sensors, this homogenous aptasensor avoided complicated labeling and purification procedure and showed magnificent sensitivity and high selectivity, which made it not only convenient but also time-saving and applicable. Furthermore, this sensing strategy also provides a promising way to develop new ECL aptasensor for other analytes by virtue of other aptamers.
研究领域:
分析化学
·
纳米技术
·
环境科学
·
传感器
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2016
·Vol. 408
·期 25
·7067-7075
·
IF 3.125
Dang Qian,Gao Hongfang,Li Zhejian,Qi Honglan,Gao Qiang,Zhang Chengxiao
A simple and sensitive electrogenerated chemiluminescence biosensor was developed to monitor matrix metalloproteinase 2 (MMP-2) by employing a specific peptide (CGPLGVRGK) as a molecular recognition substrate. Bis(2,2′-bipyridine)-4′-methyl-4-carboxybipyridine-ruthenium N -succinimidyl ester-bis(hexafluorophosphate) (Ru(bpy) 2 (mcbpy-O-Su-ester)(PF 6 ) 2 (Ru1) was used as ECL-emitting species and covalently labeled onto the peptide through NH 2 -containing lysine on the peptide via acylation reaction to form Ru1-peptide as an ECL probe. An ECL peptide-based biosensor was fabricated by self-assembling the ECL probe onto the surface of gold electrode. MMP-2 can specifically cleave the Ru1-peptide on the electrode surface, which led the partly Ru1-peptide to leave the electrode surface and resulted in the decrease of the ECL intensity obtained from the resulted electrode in 0.1 M phosphate-buffered saline (pH 7.4) containing tri- n -propylamine. The decreased ECL intensity was piecewise linear to the concentration of MMP-2 in the range from 1 to 500 ng/mL. Moreover, the ECL biosensor is successfully applied to detection of MMP-2 secreted by living cell, such as HeLa cells. Additionally, the biosensor was also applied to the evaluation of matrix metalloproteinase inhibitors. The strategy presented here is promising for other disease-related matrix metalloproteinase assay and matrix metalloproteinase inhibitor profiling with sensitivity and simplicity.
Detection of MMP-2 released from living cells by ECL peptide-based biosensor
研究领域:
生物化学
·
分析化学
·
生物技术
·
癌症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2016
·Vol. 408
·期 14
·3823-3831
·
IF 3.125
Guo Xiaofei,Wu Shijia,Duan Nuo,Wang Zhouping
A novel aptasensor labeled with Mn 2+ -doped NaYF 4 :Yb/Er upconversion nanoparticles (NaYF 4 :Yb,Er/Mn UCNPs) was employed in electrogenerated chemiluminescence (ECL) for the sensitive detection of bisphenol A (BPA). The ECL aptasensor was assembled by immobilizing the thiolated aptamers of BPA covalently on a gold nanoparticle (AuNPs)-modified electrode and pairing with complementary DNA labeled with NaYF 4 :Yb,Er/Mn UCNPs. The ECL aptasensor can not only rapidly and accurately detect BPA concentrations from 0.05 to 100 ng/mL with a detection limit of 0.037 ng/mL but also provides a new platform for ECL applications based on the use of upconversion nanoparticles as a promising alternative material.
The NaYF4:Yb,Er/Mn UCNPs combining with the BPA aptamer serving as recognition elements create a ECL platform for the sensitive detection of bisphenol A. The change in ECL signals induced by aptamer-target interactions was measured and a significant decrease in intensity was found on interaction with BPA in the concentration range of 0.05 to 100 ng/mL.
研究领域:
分析化学
·
纳米技术
·
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 3
·1-8
·
IF 5.3
Yang Shuning,Tian Li,Song Guanying,Li Huiling,Li Chao,Wu Qian,Shan Xiangyu,Zhao Lun
An electrochemiluminescence (ECL) sensor for determining bisphenol A (BPA) was prepared based on titanium dioxide (TiO 2 ) and Co-MOF. TiO 2 is a co-reaction promoter that amplifies the ECL signal in the Ru(bpy) 3 2+ -trinpropylamine (TPrA) system. When the electrode is modified with Co-MOF the ECL signal is significantly enhanced. This is because Co-MOF can not only be used as a co-reaction accelerator but also as a carrier to adsorb more luminescent substances. Possible mechanisms for amplifying the original signal through the synergistic action of the two substances are investigated. The ECL strength decreases with increasing concentrations of BPA, and the amount of BPA can be determined by the change in ECL signal strength (Δ I ). Under optimal experimental conditions, the linear range of BPA was 2.0 × 10 −10 to 2.0 × 10 −5 M, with a determination limit of 6.7 × 10 −11 M (3 σ / m ). The relative standard deviation (RSD) of the signal for ten consecutive measurements was 1.5%. The sensor can be used to detect BPA in bottled samples with recoveries of 96 to 105%. Graphical
研究领域:
分析化学
·
环境科学
·
材料科学
·
电化学
·
传感器技术
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 6
·1-11
·
IF 5.3
Zhang Hui,Ji Yahui,You Nana,Hu Xiaoping,Chen Feifei,Wang Fei,Liu Gen
Ratiometric electrochemiluminescence (ECL) assays have attracted widespread attention in biosensing owing to their precise measurements by eliminating environmental interferences. In this work, g-C 3 N 4 , gold nanoparticles, CuO and luminol were integrated onto hollow carbon spheres (HCS) in sequence to fabricate potential-resolved ECL nanoprobes. The system consists of g-C 3 N 4 as cathode ECL emitters and luminol as anode ECL emitters. The ECL of g-C 3 N 4 is quenched by CuO due to the resonance energy transfer (RET). However, after adding dopamine (DA), the ECL signal is restored due to the redox reaction between CuO and DA. Meanwhile, there is a quenching effect between DA and luminol because DA interferes with the radical reaction process of luminol. Therefore, DA causes the reciprocal changes in cathodic ECL and anodic ECL. This phenomenon can be leveraged to create a ratiometric ECL signal, enabling the quantitative detection of DA. The developed ECL sensor exhibited a sensitive detection toward DA, performing a wide linearity in the range 5.0 × 10 –4 ~ 1.0 × 10 –9 M with a low detection limit of 2.3 × 10 –11 M (S/N = 3). Furthermore, this strategy exhibited a good practicality to detect DA in human urine, providing a promising strategy in ECL bioanalysis. Graphical abstract
研究领域:
分析化学
·
生物分析
·
生物传感器
·
纳米技术
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）