Frontiers in Pharmacology
·
2020
·
IF 4.225
Huang Mei-Zhou,Zhang Zhen-Dong,Yang Ya-Jun,Liu Xi-Wang,Qin Zhe,Li Jian-Yong
Aspirin eugenol ester (AEE) is a new potential pharmaceutical compound possessing anti-inflammatory, anti-cardiovascular disease, and antioxidative stress activity. The pharmacological activities of AEE are partly dependent on its regulation of cell apoptosis. However, it is still unclear how AEE inhibits cell apoptosis on the basis of its antioxidative stress effect. This study aimed to reveal the vascular antioxidative mechanism of AEE in response to H2O2-induced oxidative stress in HUVECs and paraquat-induced oxidative stress in rats. In the different intervention groups of HUVECs and rats, the expression of ASK1, ERK1/2, SAPK/JNK, and p38 and the phosphorylation levels of ERK1/2, SAPK/JNK, and p38 were measured. The effects of ASK1 and ERK1/2 on the anti-apoptotic activity of AEE in the oxidative stress model were probed using the corresponding inhibitors ASK1 and ERK1/2. The results showed that in the HUVECs, 200 μM H2O2 treatment significantly increased the phosphorylation of SAPK/JNK and the level of ASK1 but decreased the phosphorylation of ERK1/2, while in the HUVECs pretreated with AEE, the H2O2-induced changes were significantly ameliorated. The findings were observed in vitro and in vivo. Moreover, inhibition of ASK1 and ERK1/2 showed that ASK1 plays a vital role in the protective effect of AEE on H2O2-induced apoptosis. All findings suggested that AEE protects the vascular endothelium from oxidative injury by mediating the ASK1 pathway.
研究领域:
药理学
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心血管疾病
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细胞生物学
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分子生物学
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 4
·2141
·
IF 4.9
Qi Tao,Ning Ma,Liping Fan,Wenbo Ge,Zhendong Zhang,Xiwang Liu,Jianyong Li,Yajun Yang
Aspirin eugenol ester (AEE) is a novel medicinal compound synthesized by esterifying aspirin with eugenol using the pro-drug principle. Pharmacological and pharmacodynamic experiments showed that AEE had excellent thromboprophylaxis and inhibition of platelet aggregation. This study aimed to investigate the effect of AEE on the liver of thrombosed rats to reveal its mechanism of thromboprophylaxis. Therefore, a multi-omics approach was used to analyze the liver. Transcriptome results showed 132 differentially expressed genes (DEGs) in the AEE group compared to the model group. Proteome results showed that 159 differentially expressed proteins (DEPs) were identified in the AEE group compared to the model group. Six proteins including fibrinogen alpha chain (Fga), fibrinogen gamma chain (Fgg), fibrinogen beta chain (Fgb), orosomucoid 1 (Orm1), hemopexin (Hpx), and kininogen-2 (Kng2) were selected for parallel reaction monitoring (PRM) analysis. The results showed that the expression of all six proteins was upregulated in the model group compared with the control group. In turn, AEE reversed the upregulation trend of these proteins to some degree. Metabolome results showed that 17 metabolites were upregulated and 38 were downregulated in the model group compared to the control group. AEE could reverse the expression of these metabolites to some degree and make them back to normal levels. The metabolites were mainly involved in metabolic pathways, including linoleic acid metabolism, arachidonic acid metabolism, and the tricarboxylic acid (TCA) cycle. Comprehensive analyses showed that AEE could prevent thrombosis by inhibiting platelet activation, decreasing inflammation, and regulating amino acid and energy metabolism. In conclusion, AEE can have a positive effect on thrombosis-related diseases.
研究领域:
药理学
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生物信息学
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PubMed
高影响因子
Food Bioscience
·
2026
·
IF 6.2
Xin Yang,Luzhou Cai,Yueling Luo,Lianqun Wu,Xinyue Wang,Yanping Wu,Kai Zhong,Hong Gao
Fusarium graminearum poses a major threat to global grain production due to its pathogenicity and mycotoxin contamination. This study explored the antifungal effects and characterized molecular alterations of eugenol, a plant-derived phenolic compound, against F. graminearum . An integrated multi-omics strategy combining transcriptomic and lipidomic analyses was employed to characterize stage-specific responses of conidia and mycelia to eugenol, complemented by phenotypic and biochemical assays. Eugenol exhibited pronounced dose-dependent antifungal activity, accompanied by compromised cellular integrity and disruption of lipid homeostasis. Notably, its mode of action displayed clear developmental specificity. In the conidial stage, eugenol may interfere with phosphatidylcholine transport and membrane lipid remodeling, potentially limiting the membrane supply required for polarized growth. In contrast, during the mycelial stage, eugenol exposure correlated with the induction of peroxisome-associated lipid catabolism and stress responses, likely resulting in metabolic imbalance and growth suppression. Collectively, these findings delineate a stage-specific landscape of F. graminearum responses to eugenol and propose a hypothetical model centered on lipid metabolic disruption, supporting the further development of eugenol-based antifungal strategies for controlling F. graminearum .
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高影响因子
MOLECULES
·
2026
·Vol. 31
·期 4
·669
·
IF 5.1
Jiarui Liu,Jiachang Feng,Zhefeng Xu,Jinsong Zhang,He Wang
Natural antibacterial food packaging materials endowed with environmental responsiveness are garnering substantial research interest in sustainable food preservation. This study reports the development of a pH-responsive antimicrobial composite film through encapsulation of eugenol—a natural phenolic compound—within zeolitic imidazolate framework-8 (ZIF-8). The engineered eugenol@ZIF-8 system demonstrated pH-dependent release characteristics, with cumulative release reaching 32.2% at pH 6 versus merely 0.61% at pH 7 over 4 h. Subsequent integration of this nanocarrier into a polyvinyl alcohol (PVA)/hydroxypropyltrimethyl ammonium chloride chitosan (HACC) matrix yielded a multifunctional composite film for active food packaging applications. The characterization of film revealed that while eugenol@ZIF-8 incorporation slightly compromised mechanical strength (tensile resistance decreased by 18.7%) and flexibility (elongation at break reduced to 54.3% of control), it significantly enhanced hydrophobicity (water contact angle increased to 92.5°) and thermal stability (decomposition temperature elevated by 34 °C). The composite film demonstrated synergistic antibacterial efficacy through the combined action of Zn2+ions, ZIF-8 nanostructures, and eugenol, achieving 88% inhibition againstE. coli.Practical validation through fresh noodle preservation trials confirmed the material’s effectiveness, with the optimized formulation (PVA-HACC-2% eugenol@ZIF-8, PHEZ2) extending shelf life by >5 days compared to conventional packaging. This work establishes a novel strategy for engineering intelligent ZIF-based packaging systems that respond to food spoilage microenvironments, offering significant potential for reducing food loss.
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PubMed
高影响因子
FOOD CONTROL
·
2024
·
IF 5.6
Xiaojia Wang,Jizhong Yuan,Nana Sun,Yaohuang Jiang,Yanchun Yu,Guoqiao Lai,Xiongfa Yang
Antibacterial and pH−sensitive eugenol functionalized chitosan−polyurethane hydrogels (UV−EBs) for shelf−life extension of chicken were prepared from thiol−modified chitosan (CS) and UV−curable polyurethanes grafted with eugenol and bromocresol green by UV−curable method. The antibacterial efficiency of UV−EBs is about 55% and 86% respectively after 8 h co−cultured against S. aureus and E. coli . Therefore, a contact−kill mechanism was proposed. Despite the toxicity of UV−EBs against L929 cells increased with the increment of the amount of eugenol groups, the UV−EBs prepared still have potential application in food packing and coatings. Shelf−life extension study for chicken breast demonstrated the UV−EBs can be utilized for packaging materials to extend the shelf−life of high−quality protein food such as chicken breast. The thermal stability of UV−EBs is better than eugenol because the initiate decomposition temperature ( T d5 ) of the former is in the range of 154–175 °C while that of the latter is about 134 °C. Therefore, UV−EBs have potential application in package some food that still has a high residual temperature after being cooked or heated. The UV−EBs demonstrate superior mechanical performance with tensile strength in the range of 0.77–4.90 MPa and the elongation at break in the range of 135–196%, respectively. The UV−EBs also show naked eyes visible color variation from yellow to green and finally to be Prussian blue when the pH values varied from 2 to 14. This work supplied a feasible method to develop antibacterial hydrogels for packaging materials to extend the shelf−life of high−quality protein food.
研究领域:
材料科学
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食品科学
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生物技术
·
化学
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
·
IF 6.2
Li Zhang,Xiaolin Lei,Zhaoying Wang,Zebo Liu,Jiguang Chen,Yuanyuan Zhang,Bing Cao,Yuxin Fu,Zhongping Yin,Dayong Peng,Fanxin Zeng
To discover novel preservatives for treating wood-decaying fungi, 48 novel eugenol quaternary ammonium salt derivatives were designed and synthesized. Among them, compounds 3j, 3k, 4j, 4k, 5j, 5k, and 6j showed remarkable antifungal activity against Gloeophyllum trabeum (G. trabeum), affording EC50 values ranging from 2.11–7.50 μg/mL. Notably, 3j demonstrated the most potent activity against G. trabeum (EC50 = 0.14 μg/mL), surpassing the positive control dodecyldimethylbenzylammonium chloride (DDBAC, EC50 = 3.33 μg/mL). Additionally, 3j significantly reduced wood mass loss and decay caused by G. trabeum. Mechanistic studies revealed that 3j disrupted the fungal ultrastructure, increased membrane permeability, and induced cellular leakage. Furthermore, 3j disrupted the antioxidant system and energy metabolism, resulting in the accumulation of reactive oxygen species. The multiomics data revealed the molecular mechanisms by which 3j regulated G. trabeum, including key genes, proteins, metabolites, and pathways. This study elucidates key mechanistic insights into developing a natural-product-derived wood preservative.
研究领域:
天然产物化学
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林业微生物学
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抗真菌药理学
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分子真菌学
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木材保护学
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代谢组学
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化学生物学
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PubMed
高影响因子
Journal of Agriculture and Food Research
·
2026
·
IF 6.2
Jiaxin Lin,Tao Wang,Ren Wang,Wei Feng,Xiaohu Luo,Kai Huang,Jian He
Vitamin K 2 (VK 2 ) and vitamin D 3 (VD 3 ) can synergistically promote calcium absorption and reduce the prevalence of osteoporosis. Given their hydrophobicity and environmental sensitivity, stabilizing both these vitamins is pivotal for their application. In this study, we designed shellac-gliadin composites (SGCs) with favorable amphiphilicity, yielding a self-emulsifying delivery system (SEDS) upon mild mixing with eugenol. By increasing eugenol/SGCs mass ratio, SEDS was transformed from spherical to a network-like structure, forming tunable topological nanostructures with tailorable delivery performance and bioavailability. Based on the dual-step process, VK 2 was encapsulated during shellac-gliadin assembly, while VD 3 was co-encapsulated via self-emulsification. The generated dual-vitamin self-emulsifying system (DSEDS) improved the stability under UV-light and long-term storage for both vitamins with high encapsulation capacities of 48.05 and 9.36 mg/g for VK 2 and VD 3 , respectively. The dual-step strategy introduced a novel approach for loading various fat-soluble nutrients, with potential applications in food and nutrition industry.
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高影响因子
Insects
·
2022
·Vol. 13
·期 11
·1004
·
IF 3.139
Yinjun Fan,Changzhen Zhang,Yu Qin,Xinhui Yin,Xinyi Dong,Nicolas Desneux,Hongxu Zhou
Simple SummaryBactrocera dorsalisis a highly invasive polyphagous pest of fruit and vegetable, causing severe economic loss and trade restrictions by ovipositing inside hosts. Cryptic feeding habits of larval stages, strong flight ability of adults and prevalence of insecticide resistance reduce the efficiency of chemical control. Currently, the most cost-effective tool is olfaction-based trapping. Methyl eugenol (ME), a naturally occurring compound in some plants, alone or combination with insecticides has been widely used as a male attractant to monitor and controlB. dorsalispopulations for seven decades. Intense ME selection under laboratory conditions has resulted in the evolution of non- responsiveness inB. dorsalisand field management strategies based on ME has failed to eradicate recolonization ofB. dorsalisin some islands. However, the levels of ME responsiveness inB. dorsalisfield populations in China is unknown. In this study, we found that field populations had lower ME sensitivity compared to the susceptible strain. Furthermore, the results of olfactory organs, gene expressions and the bioassays showed that odorant binding protein (BdorOBP2, BdorOBP83b) and P450 may be involved in the lower sensitivity. The findings will guide the use of lures combined with insecticides and help to exploit molecular targets for the development of new attractants.AbstractBactrocera dorsalisis a notorious polyphagous pest in China, and its management strategies largely depend on methyl eugenol (ME), which has been widely used as an attractant to monitor and eradicateB. dorsalispopulations for seven decades. However, the non-responsiveness levels in fieldB. dorsalispopulations to ME is unknown. In this study, we monitored the response to ME in field populations from the four most heavily infested provinces in China, and the results showed that the populations had lower sensitivity to ME relative to GZS susceptible strain. The percent responsiveness of the lowest sensitivity population was 5.88-, 3.47-, and 1.47-fold lower relative to the susceptible strain at doses of 1, 10, and 100 µL of ME, respectively. Gene expression analysis and inhibitor assays further revealed that odorant binding protein (BdorOBP2, BdorOBP83b) and the P450 enzyme system may be associated with the lower response to ME. To our knowledge, this work is the first to report that the P450 enzyme system confers a lower responsiveness to lure insects. These findings provided valuable insights for exploiting ME non-responsiveness to protect sterile males from ME-based control strategies and the use of lures combined with insecticides.Keywords:methyl eugenol;non-responsiveness;odorant binding protein;P450;trapping
研究领域:
昆虫学
·
分子生物学
·
农业科学
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PubMed
高影响因子
Microorganisms
·
2021
·Vol. 9
·期 5
·969
·
IF 4.128
Yi Li,Runan Zhao,Yan Li,Zhiqin Zhou
Penicillium italicum, the cause of citrus blue mold, is a pathogenic fungus that seriously affects the postharvest quality of citrus fruit and causes serious economic loss. In this study, a eugenol nanoemulsion containing limonin, an antimicrobial component from citrus seeds, was prepared using a high-pressure microfluidizer and the antifungal activity of the nanoemulsions againstP. italicumwas evaluated based on the conidial germination rate, mycelial growth, and scanning electron microscopy analysis. The results showed that the minimum inhibitory concentration and the inhibition rate of limonin-loaded eugenol nanoemulsion was 160 μg/mL and 59.21%, respectively, which was more potent than that of the limonin-free eugenol emulsion. After treatment with the nanoemulsions, the integrity of theP. italicumcell membrane was disrupted, the cell morphology was abnormal, and the leakage of nucleic acid and protein was observed. In addition, the challenge test on citrus fruits revealed that the limonin-loaded eugenol emulsion inhibited citrus infection for longer periods, with an infection rate of 29.2% after 5 days. The current research shows that nanoemulsions containing limonin and eugenol have effective antifungal activity againstP. italicum, and may be used as a substitute for inhibiting blue mold in citrus fruits.Keywords:eugenol nanoemulsion;citrus blue mold;limonin;Penicillium Italicum;antifungal activity
研究领域:
植物病理学
·
食品科学
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DOI
PubMed
高影响因子
Journal of Inorganic and Organometallic Polymers and Materials
·
2021
·Vol. 31
·期 8
·3290-3300
·
IF 3.543
Aziz Tariq,Zheng Jieyuan,Jamil Muhammad Imran,Fan Hong,Ullah Roh,Iqbal Mudassir,Ali Amjad,Khan Farman Ullah,Ullah Asmat
Bio-based epoxy resins are being used due to their green chemistry. They have better properties than petroleum-based epoxy resins. Recently, environment friendly nanomaterials have been used for different industrial applications. Cellulose nanocrystals (CNCs) are among the best naturally occurring materials. Therefore, the surface of cellulose nanocrystals are modified by eugenol-based silane coupling agent (EBSCA). Chemical composition and surface morphologies of CNCs were analyzed and characterized by FTIR, AFM, SEM, TEM and 1 H-NMR. The SEM and AFM results confirmed eugenol-based silane coupling agent was successfully grafted on cellulose nanocrystals. Modified CNCs demonstrated an excellent tensile strength (2190 MPa) and modulus (16.00 MPa), as well as storage modulus (1622 MPa) exhibited by 1wt% modified cellulose nanocrystals composites. Additionally, modified CNCs displayed hydrophobic behavior (CA = 102 ± 2°). The corresponding modified CNCs have significant applications in combination of high stiffness and strength to the epoxy resins. This study lays a foundation towards full bio-based, environment friendly polymers fabrication and consumptions most desirable in adhesive and mechanical industrial fields.
研究领域:
材料科学
·
绿色化学
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DOI
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
·
2013
·
IF 3.01
Jianyong Li,Xiaojun Kong,Xiwang Li,Yajun Yang,Jiyu Zhang
Aspirin eugenol ester (AEE) is a promising drug candidate for treatment of inflammation, pain and fever and prevention of cardiovascular diseases with less side effects and it is important to characterize its genotoxicity. In this study, the genotoxicity of AEE was assessed with two standard genotoxicity assays of the Salmonella typhimurium mutagenicity assay (Ames test) and the mouse bone marrow micronucleus assay. In the Ames test , Salmonella strains TA97, TA98, TA100, TA102 and TA1535 were treated with or without the metabolic activation with a S9 fraction from Acroclor-induced rat liver. The doses of AEE were 5 mg/plate, 2.5 mg/plate, 1.25 mg/plate, 0.625 mg/plate and 0.3125 mg/plate, respectively. In the above tested strains, mutagenicity with or without the S-9 mixture was not detected. In the mammalian erythrocyte micronucleus assay, fifty mice were divided into five groups evenly and the AEE dose at 5000 mg/kg, 2500 mg/kg and 1250 mg/kg and the cyclophosphamide dose at 40 mg/kg as a positive control, the 0.5% of CMC-Na as negative control were administered. The results showed that AEE did not induce any significant increase in micronucleated erythrocytes after 24 h ( p < 0.01). Our results suggested that AEE was non-genotoxic in vivo or in vitro .
研究领域:
药理学
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毒理学
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遗传学
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PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Qi Wang,Zhiyang Gan,Xinxin Wang,Xinying Li,Lei Zhao,Derong Li,Zhilang Xu,Changdao Mu,Liming Ge,Defu Li
A microneedle transdermal drug delivery system simultaneously avoids systemic toxicity of oral administration and low efficiency of traditional transdermal administration, which is of great significance for acne vulgaris therapy. Herein, eugenol-loaded hyaluronic acid-based dissolving microneedles (E@P-EO-HA MNs) with antibacterial and anti-inflammatory activities are developed for acne vulgaris therapy via eugenol transdermal delivery integrated with photothermal therapy. E@P-EO-HA MNs are pyramid-shaped with a sharp tip and a hollow cavity structure, which possess sufficient mechanical strength to penetrate the stratum corneum of the skin and achieve transdermal delivery, in addition to excellent in vivo biocompatibility. Significantly, E@P-EO-HA MNs show effective photothermal therapy to destroy sebaceous glands and achieve antibacterial activity against deep-seated Propionibacterium acnes (P. acnes) under near-infrared-light irradiation. Moreover, cavity-loaded eugenol is released from rapidly dissolved microneedle bodies to play a sustained antibacterial and anti-inflammatory therapy on the P. acnes infectious wound. E@P-EO-HA MNs based on a synergistic therapeutic strategy combining photothermal therapy and eugenol transdermal administration can significantly alleviate inflammatory response and ultimately facilitate the repair of acne vulgaris. Overall, E@P-EO-HA MNs are expected to be clinically applied as a functional minimally invasive transdermal delivery strategy for superficial skin diseases therapy in skin tissue engineering.
研究领域:
药学
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皮肤科
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纳米技术
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治疗工程
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生物医学工程
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）