LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Xiuying Liu,Binbin Guan,Min Yang,Xinwen Bai,Wei Zhang,Pingping Wang,Zaixi Shu,Yiwei Tang,Lijie Zhu
In this study, a novel intelligent water-absorbent cellulose pad with a specified area was developed for the visual and non-destructive monitoring of fish freshness. The pad's substrate was composed of wood pulp cellulose (WPC), and the specified area contained a ratiometric mixture of brilliant blue (BRI) and alizarin (Alz)., fixed with Polyvinyl alcohol (PVA). During the freshness monitoring process, the Alz/BRI-PVA WPC pad exhibited a color transition from green to brown, followed by a shift to purple-red, corresponding to the increasing levels of total volatile base nitrogen (TVB-N) and total viable count (TVC). After testing, a BRI/Alz ratio of 1:8 was selected as the optimal ratio. Compared to the volatile gas-based sensing tags, the pad developed in this study demonstrated a high sensitivity of 53.4% by interacting with the sample exudate to produce signal changes. This study demonstrates the feasibility of using the developed dual-functional pad for water absorption and freshness indications.
研究领域:
食品科学
·
材料科学
·
传感器
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产品关联
DOI
高影响因子
COMPUTERS AND ELECTRONICS IN AGRICULTURE
·
2025
·
IF 8.9
Xianbao Xu,Bingxiong Wang,Zhuangzhuang Du,Zhuangzhuang Bai,Shuaixing Wang,Cong Wang,Daoliang Li
Dissolved oxygen is crucial in aquaculture, as it directly affects the health and survival of aquatic organisms. With increasing demand for detailed environmental data to enable three-dimensional monitoring, the use of lightweight, compact sensors in underwater mobile devices has become essential. In this study, a flexible dissolved oxygen sensor was developed, and the sensor features a compact, two-electrode array, encapsulated in PDMS to provide flexibility and secure attachment. A multi-electrolytic cell array structure amplifies the current signal from the sensing electrodes. Efficient encapsulation of the electrodes and electrolyte prevents displacement by water flow, ensuring stable and reliable detection performance. The results demonstrate the sensor’s stability, with a detection error of less than 0.2 compared to commercial sensors. The mean absolute error across four different aqueous environments was 0.083. Moreover, the sensor maintained excellent performance over a 14-day testing period. We also successfully integrated the sensor into a bionic robotic fish, enabling three-dimensional detection of dissolved oxygen in the environment. This research advances the development of compact, high-performance underwater sensors, particularly for applications in aquaculture.
研究领域:
环境科学
·
生物工程
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产品关联
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Qin Hu,Qiuyan Mao,Yikun Cui,Shiyun Gong,Lixia Xiao,Xiaojuan Gong,Tianzhu Guan,Zhen-quan Yang
This work made the first attempt to construct a florescence assay based on CDs@FMs (carbon dots-based fluorescence microspheres) for ultrasensitively probing malachite green (MG). Through static quenching and inner filter effect (IFE), the 581-nm emission peak of CDs@FMs could be significantly suppressed by MG. Our results demonstrated two good linear fluorescence responses for MG in the concentration scopes of 0.01–0.1 μM and 0.1–10.0 μM, respectively. Strikingly, the detection limits (LODs) achieved were down to picometer (56.7 pM) and nanometer levels (0.57 nM). Furthermore, this method exhibited good consistency with the HPLC results when applied for quantitatively screening MG from fish samples. Our current research highlights a promising application of CDs@FMs for enhanced MG probing in aquatic products with the merits of rapidity, simplicity, high accuracy and stable signal readout.
研究领域:
分析化学
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食品安全
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环境科学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Shuo Shi,Xiaodan Wu,Ying Wang,Wenxin Li,Hao Zhang,Xinjiang Lou,Xiufang Xia,Weiwei Liang
In this study, hydrophobic sodium alginate/anthocyanin/cellulose nanocrystal indicator films were fabricated by incorporating nanosilica (NS) as a waterproofing layer. The concentrations and formation methods (spraying (S), coating (C), and impregnation (I)) of the NS layer (denoted as NSS, NSC, NSI, respectively) were optimized. The results indicated that the optimum concentration of the NS layer was 5 % at a water contact angle (WCA) 110.5°. Further, Fourier transform infrared spectra showed the presence of Si O Si and Si CH 3 groups in the NSS, NSC, and NSI films, and X-ray diffraction spectra indicated that original structures of these films were disordered. Moreover, the surface morphology, mechanical properties, and light transmission were affected by the NS layer, and the optimal layer was found to be NSI. After 10 days of storage at 100 % humidity, the NSI film exhibited low water vapor adsorption (37.22 g) and permeability (0.1484 g/m·s·Pa·10 −11 ) and a high WCA (110.2°). In addition, the NSI film exhibited a visible color shift with an increasing pH of the buffer solution. A monitoring test of fish freshness showed that the NSI film displayed a distinctive color change corresponding to fish spoilage during 14 days of storage. This indicates that NSI has high potential in indicator film applications.
研究领域:
食品科学
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材料科学
·
化学
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产品关联
DOI
PubMed
高影响因子
Food Packaging and Shelf Life
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2024
·
IF 8.5
Yahong Han,Mingrui Zhou,Jiajie Deng,Cheng Cheng,Zihan Xu,Wenfu Hou,Yang Yi,David Julian McClements,Shuai Chen
Recently, intelligent packaging integrated with natural colorants has garnered interest for its capability to assess the freshness of stored food and delay its spoilage. However, the poor dispersibility and stability of natural pigments limit their application. This study addresses these issues by encapsulating natural pigments in core-shell protein-polysaccharide nanoparticles to enhance their stability and dispersibility. Using curcumin and anthocyanin as examples, pigment-loaded nanoparticles were synthesized using antisolvent precipitation to create zein particles, which were then coated with chondroitin sulfate through electrostatic layer-by-layer deposition. This method produced spherical anionic nanoparticles with high encapsulation efficiency (Curcumin: 91.93 %, Anthocyanin: 85.85 %). These nanoparticles were incorporated into carrageenan-based composite films, which acted as freshness indicators and preservatives. The films' color shifted from green-brown ( L =33.47, a =9.46, b =11.09) to red-brown ( L =22.80, a =13.83, b =2.11) with increasing nanoparticle concentration (from 20 % to 40 % w/v), and their uniform microstructure demonstrated good compatibility with the biopolymer matrix. The films were tested as freshness indicators and preservatives for packaged fish, showing improved freshness detection and preservation effectiveness with higher nanoparticle content. These biodegradable materials are expected to be intelligent packaging solutions for the food sector.
研究领域:
食品科学
·
材料科学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Haotian Zhang,Xiaoying Chen,Hua Rong,Baojia Chen,Junfeng Guang,Qing Pan,Xiaobo Wen,Lijun Ning
Unlike predictability in mammals, the responses of PPARα activation treatment in fish varies between different fish species. Existing results indicated that the varied effects may result from fish feeding habits difference within their varied dietary protein content, while the exact mechanism remains obscure. In this study, we conducted an in vivo 8-week trial of low protein 30 % (LP) or high protein 38 % (HP) diet fed tilapia (8.83 ± 0.02 g) supplemented with or without fenofibrate and in vitro 24-h of BCAA (leucine, valine and isoleucine) treated primary cell trial, aiming to reveal the unknown mechanism. The in vivo results showed that, in LP group, fenofibrate significantly increased serum levels of TG, glucose, AST, ALT, MDA and liver TG ( P < 0.05), while decreased liver, muscle and serum BCAA, liver mitochondria quantity, glucose tolerance ( P < 0.05). Moreover, fenofibrate notably lifted the hepatic bckdha , bckdhb , mff , fis , fas and pparγ expression ( P < 0.05), while decreased genes expression of pgc1α , tfam , parkin , pink , foxo3a, sod2, cat , ir and irs ( P < 0.05). No great impact was found in aco , cyp4a3 and cytb ( P > 0.05). In HP group, fenofibrate improved lipid and glucose metabolism along with unaltered BCAA, increased expression of aco , cytb , cpt1a , pparβ , foxo3a , sod2 , nrf1 , mfn1 and fis ( P < 0.05). In vitro findings revealed that the PGC1α and PPARα could be activated by BCAA, particular for leucine ( P < 0.05). Taken together, we conclude that: 1) increased lipid deposition, lipid peroxidation, liver damage and glucose intolerance in fenofibrate-LP group could be ascribed to decreased BCAA and done through PGC1α/FOXO3, PGC1α/MQC regulation; 2) improved lipid-lowering and glucose tolerance in fenofibrate-HP group would be attributed to the enhanced PGC1α/MQC, organelles proliferation and fatty acid β-oxidation.
研究领域:
分子生物学
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营养学
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代谢组学
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产品关联
DOI
PubMed
高影响因子
Water
·
2024
·Vol. 16
·期 17
·2531
·
IF 3
Zhenjiang Yang,Daoquan Zhao,Jinxing Gu,Ran Wu,Bianzhi Liu,Guangqing Yu,Pengsheng Dong,Xiaocheng Huang,Ming Li,Guoxi Li
To investigate the toxic and environmental effects of prometryn, a laboratory experiment was performed on coexistent fish and algae. The body weight and length ofCarassius carassius,Microcystis aeruginosa(M. aeruginosa) densities and water quality were measured continuously for 92 days. It was observed that fish growth was significantly inhibited by prometryn. This can be partly attributed to the adverse effects of prometryn on the antioxidant system of fish; the activities of superoxide dismutase (SOD) and catalase (CAT) in crucian carp were significantly inhibited by prometryn. The growth ofM. aeruginosawas greatly inhibited by prometryn (p< 0.05), and the adverse effects of prometryn onM. aeruginosaindirectly impacted water qualities. The body weight and length ofCarassius carassiusfirst increased and then tended to be stable with increasing electrical conductivity (EC) values; their quantified relationship was established based on the Gompertz and Logistic equations (R2= 0.920–0.989). Based on the above results, we concluded that the toxic effects of prometryn can impact the antioxidant system of fish and algae which in turn affects their growth performance, and have an indirect impact on water quality. The application of equations made it realizable to obtain a more detailed interpretation of the processes involved in these biological–abiotic interactions.
研究领域:
环境科学
·
毒理学
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产品关联
DOI
高影响因子
Inorganic Chemistry Frontiers
·
2022
·Vol. 9
·期 10
·2182-2193
·
IF 7.779
Peng Liu,Tian Xia,Suofu Nie,Qiaoxia Guo,Chunming Xu,Baojian Shen
Y zeolite is presently used as an important active component in oil refining catalysts. The efficient synthesis of NaY zeolite still faces many huge challenges. Herein, a novel method for the rapid synthesis of nano-plate-like small crystal-sized NaY zeolite through an artificial-fish-reef-breeding negative crystal strategy is reported. It was discovered that cationic polyacrylamide (CPAM) could act as an artificial fish-reef to breed negative crystals, which promoted NaY zeolite nucleation and accelerated the crystallization of NaY zeolite. The crystallization time of NaY zeolite was shortened by 9 h. The relevant crystallization mechanism was investigated. Nano-plate-like (10–50 nm) small-sized (100–200 nm) NaY zeolite crystals with exceptional thermal stability and a large total volume (0.447 cm3 g−1) were synthesized. After steam treatment, the nano-plate-like small-sized USY-CPAM zeolites possessed reasonable acid sites distribution, endowing them with superior cracking activity for heavy reactant molecules.
研究领域:
材料科学
·
催化化学
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DOI
高影响因子
AQUACULTURE
·
2018
·
IF 2.71
Shengli Fu,Mingmei Ding,Yanjian Yang,Jingrong Kong,Yuan Li,Zheng Guo,Anli Wang,Jianmin Ye
Caspases are a family of proteases involved in many important biological processes, including inflammation, cell differentiation and apoptosis. Among them, caspase-6 plays an important role in the execution phase of the apoptotic death cascade. In this study, the full-length of puffer fish caspase - 6 ( Pfcasp - 6 ) was cloned and characterized, containing an open reading frame of 912 bp, a 5′-UTR of 168 bp and a 3′-UTR of 632 bp with a poly (A) tail. The Pfcasp - 6 encoded a polypeptide of 303 amino acids with an estimated molecular mass of 34.09 kDa and a predicted isoelectric point of 5.35. Phylogenetic analysis indicated that Pfcasp-6 was highly homology to that of other teleostean species. Spatial mRNA expression analysis revealed that the ubiquitous expression of Pfcasp - 6 in all examined tissues, with high expression in skin, followed by gill, intestine, kidney , blood, heart and spleen. After the challenges of high temperatures and Aeromonas hydrophila , the transcript expressions of Pfcasp - 6 were significantly up-regulated in blood and spleen. In addition, cellular localization analysis indicated that the distribution of Pfcasp-6 was in the cytoplasm and nucleus. Overexpression of Pfcasp-6 was able to trigger a significant level of cell death in HeLa cells, suggesting its putative role in cell apoptosis. Taken together, the results of this study suggested that Pfcasp - 6 might be an important component in the caspases cascade, and was likely to get involved in the response to different stress in puffer fish.
研究领域:
分子生物学
·
免疫学
·
鱼类生理学
·
基因表达
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DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2023
·
IF 10.7
Hulin Geng,Wanyi Sun,Shengnan Zhan,Ru Jia,Qiaoming Lou,Tao Huang
In this study, the influence of glycosylation with κ-Carrageenan (κC), high methoxyl pectin (HMP), d -Galactose (Gal) on the gelling, rheological properties and mechanisms of fish gelatin (FG) were comparatively investigated. Results showed that κC was easiest to glycosylated with FG in comparison to HMP and Gal, also had the most significant improvement on the gelling properties and viscosity of FG. HMP was most difficult to glycosylated FG, but still had an excellent enhancement on viscosity. The results of intrinsic fluorescence, FTIR, X-Ray and SEM indicated that proper glycosylation changed the conformation structure and improved the contents of hydrogen bonds and α-helix, stabilizing the gels network. LC-MS/MS results showed that the main glycosylation sites were lysine and arginine amino groups, which was regarded as N-terminal glycosylation. In conclusion, proper N- glycosylation could significantly improve the gelling and rheological properties of FG. This study may provide a new guideline for modification FG to expand its application.
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DOI
高影响因子
ACS Food Science & Technology
·
2023
·
IF 2.3
Xiaxi Pan,Guixin Chen,Ting Xu,Lingyue Kong,Xinying Li,Defu Li,Changdao Mu,Zhilang Xu,Liming Ge
The development of packaging films with food preservation and monitoring functions is of great significance and applications. Herein, gelatin was combined with rosmarinic acid (RosA) and blueberry anthocyanin extract (BAE) to fabricate RosA-gelatin/BAE (RGA) films with pH-responsiveness and long-term antioxidant and antibacterial activities. The films have better surface hydrophobicity and antiswelling performance than that of pure gelatin film. The films show good ductility and the elongation at break of the films can be easily adjusted in the range of 1.01–292.19% by adjusting the content of BAE. The films can block ultraviolet light, scavenge the ABTS+ radical, and kill bacteria almost 100%. Significantly, the films showed good pH/NH3 responsiveness as an obvious color change. Comparing with polyethylene film, there was a reduction of around 60.8% in the release of volatile base nitrogen compounds from crucian in storage using RGA5. Overall, these films may be useful for food preservation and real-time quality monitoring.
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产品关联
DOI
高影响因子
Analytical Methods
·
2023
·Vol. 16
·期 1
·114-121
·
IF 3.1
Liufeng Zhang,Juan Sun,Yinmeng Ding,Li Li,Jing Liu
Methyltestosterone is one of the banned drugs in aquaculture, and it should be monitored in food-producing animals. 17α-Methyl-5β-androstane-3α,17β-diol, as the main metabolite of methyltestosterone in vertebrates, could be used as another marker for controlling the administration of methyltestosterone, due to its high residual concentration and slow elimination rate. In this study, an analytical method based on gas chromatography-mass spectrometry (GC-MS) was developed and validated for the simultaneous determination of methyltestosterone and its main metabolite in fish. After pretreatment by liquid–liquid extraction with n-hexane and solid phase extraction with C18 and NH2 columns, the target analytes in the muscle tissues were extracted and concentrated, and the influence of the sample matrix was eliminated. Then, the prepared samples were separated and detected with GC-MS in the selected ion monitoring (SIM) mode. Methyltestosterone-D3 was chosen as the internal standard for quantitation. After optimization, the limits of detection for methyltestosterone and 17α-methyl-5β-androstane-3α,17β-diol were 20 μg kg−1 and 15 μg kg−1, respectively. The limits of quantitation were both 50 μg kg−1. The calibration curves showed good linearity in the concentration range from 50.0 ng mL−1 to 500.0 ng mL−1. The correlation coefficients of methyltestosterone and 17α-methyl-5β-androstane-3α,17β-diol were more than 0.9990. The recoveries of the analytes in real samples were in the range of 99.7–116.6% with the relative standard deviation of 5.2–8.3%. The established method could meet the demand for simultaneous detection of methyltestosterone and its major metabolite, and it could be used to provide more information on the abuse of methyltestosterone in food-producing animals.
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）