Journal of Analysis and Testing
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2022
·Vol. 6
·期 2
·111-119
·
IF 0
Guo Xin,Deng Xin-Chun,Zhang Ya-Qing,Luo Qin,Zhu Xiao-Kang,Song Yang,Song Er-Qun
Rapid and accurate detection of pathogenic bacteria of Staphylococcus aureus ( S. aureus ) is of importance in the field of clinical research and practical application. Herein, we report a magnetic relaxation switching (MRS)-based strategy for detecting S. aureus in complex samples via the assistance of signal amplification system of liposome. In this design, vancomycin (Van) modified 200 nm magnetic beads (MB 200 -Van) and biotinylated immunoglobulin (Biotin-IgG) were employed as capture probes for S. aureus which following labelled by biotinylated liposomes loading with glucose molecule (Biotin-liposome@Glu, BLGs) based on the affinity of biotin with streptavidin. The released glucose molecules combining with glucose oxidase generated Fe 3+ , causing transverse relaxation time ( T 2 ) signal change. The combination of dual recognition of S. aureus , liposome assisted signal amplification, and the MRS-based assay achieved direct and specific detection of S. aureus in blood and abscess samples. The as-proposed method for S. aureus assay in this study shows potential application for infectious diagnosis in clinical.
研究领域:
临床研究
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生物医学工程
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分子诊断
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传染病
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产品关联
DOI
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Xi Chen,Yunfeng Chen,Songrong Li,Cheng Xue,Dongfang Liu,Wenli Huang
Phthalic acid esters (PAEs) pose serious health risks to humans and are difficult to remove by conventional sewage treatment processes. Herein, Fe 2 Mo 3 O 8 /MoO 2 /Mo 2 N composite (FMON) derived from bimetallic organic framework was used to activate peroxymonosulfate (PMS) for dibutyl phthalate (DBP) degradation. FMON exhibited an excellent catalytic effect with DBP removal efficiency of 92.4 % within 60 min. Mo 2 N showed a superior ability in promoting Fe 3+ /Fe 2+ redox cycle. The reduction efficiencies of Fe 3+ by Mo 2 N/MoO 2 composite and MoO 2 were 67 %–94 % and 11 %–23 %, respectively, in the homogeneous comparative experiment. Moreover, the ratio of Fe 2+ /Fe 3+ in FMON increased significantly after the catalytic reaction. Fe element doping also enhanced the reuse stability and reduced the metal ion leaching of the catalyst. The degradation of DBP was mediated by a variety of reactive species, of which SO 4 − , OH and O 2 − were dominant. Fe element, Mo element and O vacancies were confirmed to exert significant contributions as active sites in the catalytic process. Three degradation pathways of DBP were obtained by DFT calculation and HPLC-MS analysis. And the toxicity of DBP degradation products was significantly reduced based on the assessment result of Toxicity Estimation Software Tool (T.E.S.T.). This study explored a novel Fe/Mo bimetallic catalyst with friendly Fe 3+ /Fe 2+ cycling for environmental catalysis and provided new ideas for the treatment of PAEs wastewater.
研究领域:
环境催化
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废水处理
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材料科学
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产品关联
DOI
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2024
·
IF 4.2
Lei Diao,Jing Gao,Yuxun Zhou,Liping Wang,Xiaowei Yang,Peng Li,Jingying Zhai,Yang Ma,Huijie Chen
Fenpropathrin (FPT) is a synthetic pyrethroid insecticide, the persistence and accumulation in water of which could cause harmful effects on vulnerable groups like aquatic creatures, particularly posing significant risks to fish immune systems. This study aimed to investigate how environmentally relevant FPT concentrations (10–1000 μ/M) affect lipid peroxidation and Fe 2+ metabolism in Cyprinus carpio head kidney lymphocytes, and its relationship with oxidative stress and immunotoxicity. Firstly, CCK-8 results demonstrated that FPT caused a significant increase in lymphocyte death. Secondly, lymphocytes exposed to FPT could lead ferroptosis in lymphocytes, accompanied by evidence of the Fe 2+ transporter imbalance, lipid peroxidation, Fe 2+ accumulation and ferroptosis related protein increment. Thirdly, we found that FPT esposure leads to a decrease in ATP, mitochondrial DNA and NADPH/NADP + levels, and the mRNA associated with mitochondrial function-related genes (Fis1, Drp1, and OPA1) in lymphocytes. Additionally, FPT induced the increased the levels of inflammatory genes (TNF-α, IFN-γ, and IL-6) in head kidney lymphocytes. Importantly, exposure to FPT induced oxidative stress to produce intracellular ROS, disrupting the function of the CncC signaling pathway and expression disorder of xenobiotics detoxification (CYP 450 family) genes. Notably, Treatment with NAC (a ROS inhibitor, 5 μM) demonstrated that inhibiting ROS alleviated FPT-induced lymphocyte ferroptosis and inflammatory response via the ROS/CncC-xenobiotics signaling pathway. These findings not only introduces a novel approach to investigating the immunotoxicity of FPT but also offers critical insights into mitigating the adverse effects of FPT on aquatic animal health.
研究领域:
环境毒理学
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免疫学
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水生生物学
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分子生物学
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DOI
PubMed
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 15
·8963-8969
·
IF 3.361
Zhiying Li,Congshu Li,Huiyun Yan
Designing exceptional probes to detect minute quantities of chromium(VI) is of huge importance for the safety and health of the human race. In this study, a green fluorescence emission probe (polyethylene Schiff base-chelated Fe2+ complex) was synthesized by a one-pot synthesis method for the highly selective and sensitive detection of Cr(VI) in an aqueous solution. The complex of polyethylene Schiff-Fe2+ was fully characterized, and it displayed satisfactory stability in the aqueous solution. The fluorescence emission could be quenched specifically by the introduction of Cr(VI) via the oxidation of the Fe2+-centered polyethylene Schiff base complex. The fluorescence intensity decreases linearly with the concentration of Cr(VI), and the corresponding detection limit was calculated to be 0.18 μM. Thus, the obtained fluorescence detection system could be used for Cr(VI) detection in tap water. These features provide potential uses of the as-prepared polyethylene Schiff-Fe2+ complex as a sensor for environmental applications.
研究领域:
分析化学
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环境科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
Materials Today Communications
·
2023
·
IF 3.8
Siyuan Zhang,Xinjian Cheng
Developing water-soluble fluorescent probes has important scientific and practical significance. In this work, coumarin derivatives bearing -OH were obtained through the classical Knoevenagel reaction firstly. Secondly, -C C- on allyl bromide was introduced into coumarin by Williamson ether formation method. Thirdly, hydrazine hydrate was added to the coumarin derivatives, a polymerizable small molecular coumarin fluorophore containing probe was obtained successfully. And, the -COOH on di-thioester underwent an esterification reaction with –OH groups on β-cyclodextrin(β-CD) to prepare oligomer based reversible addition-fragmentation chain transfer(RAFT) reagents. Finally, three copolymer probes β-CD-PMAA-Coumarin, β-CD-PMAm-Coumarin, and β-CD-PHEMA-Coumarin were prepared via RAFT polymerization , in which C C-coumarin was used as the functional monomer and methacrylamide(MAm)/methacrylic acid (MAA)/hydroxyethyl methacrylate (HEMA) as the main monomers. The MALDI-TOF mass tests showed that the macromolecular level probes were successfully prepared. These polymer probes have molecular weights up to 5.4 kDa and have narrow molecular weight distributions (PDI≤1.31). With the assistance of water-soluble long-chained PMAm/PMAA/PHEMA and hydrophilic β-CD, the coumarin containing macromolecular fluorescent probes could be dissolved in water readily. These water soluble polymer fluorescent probes could recognize Fe 3+ /Fe 2+ and Cu 2+ ions with high sensitivity. The detection limit of Fe 3+ could be as low as 0.34 μM. This study successfully developed a promising technique for producing highly selective, fully water-soluble fluorescent macromolecular probes.
研究领域:
化学
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材料科学
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分析化学
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产品关联
DOI
高影响因子
Catalysts
·
2025
·Vol. 15
·期 9
·888
·
IF 4
Qiongqiong He,Zhaoyang Song,Shaomeng Huang,Ruize Gao,Chao Han,Zhenyong Miao
Heterocyclic compounds in high-salinity wastewater are highly resistant to degradation, posing significant treatment challenges. A hybrid micro-nano bubble Fenton system (FT-MNBs) was developed to enhance Fe2+activation via interfacial effects. The FT-MNBs achieved a significantly higher indole degradation rate (0.0380 min−1) compared with micro and nano bubbles (MNBs) alone (0.0046 min−1) and conventional Fenton (0.01008 min−1). In real coking wastewater with a total dissolved solid (TDS) content of 3.266 g/L, FT-MNBs achieved COD removal efficiencies of 93.42% (initial COD 200 mg/L) and 72.54% (initial COD 10,000 mg/L), demonstrating excellent adaptability and efficiency in treating refractory high-salt organic wastewater. Electron spin resonance confirmed •OH as the main reactive species. Molecular simulations revealed that the MNB interface enhances the adsorption energy of Fe and H2O2, alters the Fe 3d orbital to better overlap with the O–O 2p orbital, and increases electron density—thus promoting O–O bond cleavage and free radical generation. The FT-MNBs not only enhances reaction kinetics but also offer scalability and energy efficiency, showing great potential for advanced industrial wastewater treatment.
研究领域:
环境科学
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化学工程
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高级氧化技术
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水处理技术
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活性氧物种
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产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2022
·
IF 9.136
Yu-qiong Gao,Yan-yan Rao,Han Ning,Ju-xiang Chen,Qian Zeng,Fu-xiang Tian,Nai-yun Gao
The degradation of diclofenac (DCF), a commonly used nonsteroidal anti-inflammatory drug, by Fe 2+ /chlorine and Fe 2+ /PMS processes was comparatively investigated. The influencing factors of pH, Fe 2+ and oxidant dosage on DCF degradation efficiency were evaluated and optimized. Typically, with an initial solution pH of 5, an Fe 2+ dosage of 70 μM, and a chlorine or PMS dosage of 70 μM, the degradation efficiency of DCF using the Fe 2+ /chlorine and Fe 2+ /PMS processes reached 94.2% and 79.7%, respectively, within 10 min. Quenching tests indicated the coexistence of •OH and RCS in the Fe 2+ /chlorine system, and both SO 4 ●– and •OH were identified in the Fe 2+ /PMS system. In addition, Fe(IV) was also identified to participate in DCF degradation in two systems based on the oxidation of PMSO to PMSO 2 . The degradation products and pathways of DCF were elucidated by LC/TOF/MS analysis and DFT calculations. It was assumed that the degradation pathway of DCF involves hydroxylation, dechlorination, decarboxylation, cleavage of the C-N bond, and chlorine substitution. In addition, trichloromethane (TCM), dichloroacetaldehyde (DCAL), dichloroacetonitrile (DCAN) and trichloronitromethane (TCNM) were also detected in the Fe 2+ /chlorine process, and these chlorinated DBPs initially increased and then decreased with prolonged reaction time, indicating that Fe 2+ /chlorine is capable of controlling DBPs formation. Finally, the acute and chronic toxicity of DCF and its degradation products were evaluated by the ECOSAR program.
研究领域:
环境科学
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化学工程
·
毒理学
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DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2022
·
IF 14.224
Suzhou Xu,Can Liu,Xuping Jiang,Xinyu Wang,Shijin Zhang,Yanting Zhang,Qingguo Wang,Weiling Xiong,Jing Zhang
The performance and mechanisms of a titanium carbide (Ti 3 C 2 ) MXene modified Fe 3+ /hydrogen peroxide (H 2 O 2 ) system were compared in detail under dark and visible light conditions, with a new mechanism proposed for the reaction and reduction of MXene by Fe 3+ . Using Bisphenol A (BPA) as the target pollutant, the degradation of BPA by the Fe 3+ /H 2 O 2 system was improved after adding MXene in the dark, and the degradation rate of BPA was ≥ 95 % within 12.5 min under visible light, six times higher than that in the dark. Fe 2+ was ascertained to be the effective component responsible for H 2 O 2 activation to produce ·OH. SEM , XPS, ICP, XRD, and FTIR spectroscopy , analyses show that MXene and Fe 3+ form a complex, and then MXene reacts with Fe 3+ by breaking the Ti–C bonding to accelerate the Fe 3+ /Fe 2+ cycle. MXene uses photogenerated electrons to promote this reaction under visible light. In addition, quenching experiments and electron spin resonance spectroscopy results show that ·OH and O 2 •− are the main reactive oxygen species under visible light, while ·OH is the main active species in the dark. MXene thus effectively uses O 2 to form O 2 •− under visible light and promotes the Fe 3+ /Fe 2+ cycle. This study provides a theoretical basis for the combination of visible light catalysis and the advanced oxidation process of a Ti 3 C 2 MXene.
研究领域:
环境科学
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化学工程
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材料科学
·
高级氧化过程
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Zhichao Liu,Shengnan Wang,Wanying Li,Yang Tian
Iron ions, as a main component of intracellular labile iron, not only play an important function in oxygen transport, enzymatic reactions, and electron transport but also are vitally important in oxidative stress. In this work, we developed a ratiometric fluorescent biosensor for ferrous ion (Fe2+), in which gold nanoclusters (AuNCs) were synthesized as a stable fluorescent probe and a ligand (FeL) was designed for specific recognition of Fe2+ and conjugated onto AuNCs (AuNC@FeL). Meanwhile, water-soluble sulfocyanine 7 N-hydroxysuccinimide ester (Cy7 NHS ester) was immobilized onto AuNC@FeL as a reference element. The developed ratiometric fluorescent nansosensor displayed good linearity with the concentration of Fe2+ in the range of 1–105 μM, and detection limit was achieved down to 210 nM. In addition, this nanosensor responded to Fe2+ in less than 1.23 s and showed high selectivity against other metal ions, amino acids, and reactive oxygen species. With the advantages of high selectivity and accuracy, as well as quick response and long-term stability, this organic–inorganic ratiometric fluorescent probe was successfully applied in real-time biosensing and bioimaging of Fe2+ in neurons and HepG2 cells. By use of this tool, it was found that the increasing concentration of Fe2+ in live cells was closely related to oxidative stress.
研究领域:
生物成像
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细胞生物学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2025
·
IF 9.6
Weibo Zhang,Chong Chen,Ran Wang,Yixuan Li,Yinhua Zhu,Jiazeng Sun,Yongting Luo,Junjie Luo,Liang Zhao,Yanan Sun,Huiyuan Guo,Hao Zhang,Bing Fang,Yao Hu,Rong Liu,Xiaoyu Wang,Jiayue Guo,Ping Liu,Peng An,Pengjie Wang,Juan Chen,Fazheng Ren
Human serum albumin (HSA) plays an indispensable role as a carrier of endogenous and exogenous substances in vivo. The efficiency of endocytosis determines the transport capacity. However, the potential for enhancing the efficiency of HSA endocytosis remains largely unknown. This research represented a substantial advancement, revealing that Fe 2+ markedly increased HSA endocytosis. Moreover, Fe 2+ facilitated the endocytosis and transport of HSA in vivo. The enhancement in HSA uptake facilitated by Fe 2+ is markedly decreased following iron chelation, indicating a specific interaction between Fe 2 ⁺ and HSA that promoted endocytosis. Moreover, the study elucidated that FcRn and caveolin regulated HSA endocytosis under normal conditions. Yet, in the presence of Fe 2 ⁺, endocytosis shifted toward clathrin- and caveolin-enriched membrane domains, requiring both divalent metal transporter 1 (DMT1) and FcRn for efficient process completion. Thereby a new pathway is discovered for Fe 2+ -dependent HSA endocytosis involving DMT1 and clathrin. This findings highlighted the crucial role of iron in enhancing HSA endocytosis and transport, establishing Fe 2+ as a vital endocytic enhancer that required binding to HSA to substantially improve its uptake. This insight into the mechanism of HSA endocytosis not only expands the understanding of protein transport but also opens new avenues for therapeutic intervention.
研究领域:
生物化学
·
细胞生物学
·
医学
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产品关联
DOI
PubMed
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
·
2017
·
IF 9.446
Tianyuan Xu,Runliang Zhu,Gangqiang Zhu,Jianxi Zhu,Xiaoliang Liang,Yanping Zhu,Hongping He
Heterogeneous photo-Fenton catalysts generally show very weak photo-Fenton activity at near-neutral pH because of the relatively low rate of Fe 2+ regeneration. The aim of this work is to develop novel heterogeneous photo-Fenton catalysts, which can accelerate the regeneration of Fe 2+ and thus have superior photo-Fenton activity at near-neutral pH. In this study, ferrihydrite (Fh) was modified with BiVO 4 to synthesize BiVO 4 /Fh composites, and we expected that the photogenerated electrons from BiVO 4 would accelerate the regeneration of Fe 2+ on Fh. Mechanistic investigation of H 2 O 2 consumption showed that the introduction of BiVO 4 promoted the decomposition of H 2 O 2 into reactive oxygen species (ROS). In addition, the presence of BiVO 4 deterred O 2 − production and accelerated the formation of OH, according to the results of ROS formation, as shown with EPR spectroscopy and fluorescent probes tools. Furthermore, the Fe 2+ concentration on the surface of BiVO 4 /Fh was higher than that on Fh based on the results of XPS characterization and quantitative measurement with a 1,10-phenanthroline spectrophotometric method. These results demonstrated that the introduction of BiVO 4 can accelerate the reduction of Fe 3+ to Fe 2+ by transferring photogenerated electrons from BiVO 4 to Fe 3+ on the surface of Fh. Meanwhile, a higher decolorization efficiency of acid red 18 by BiVO 4 /Fh than by Fh and pure BiVO 4 verified that the introduction of BiVO 4 can significantly enhance the photo-Fenton catalytic activity of Fh even at near-neutral pH. Overall, our work has important implications for understanding the photo-Fenton mechanisms on semiconductor modified heterogeneous photo-Fenton catalysts and confirms that the introduction of a semiconductor can enhance the photo-Fenton catalytic activity of heterogeneous photo-Fenton catalysts in acidic and neutral pH.
研究领域:
环境科学
·
材料科学
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DOI
高影响因子
Journal of Nanomaterials
·
2019
·
IF 2.233
Jun Li,Shiyao Ma,Xincai Xiao,Dan Zhao
This paper reports the preparation of green-emissioned CDs by a one-step hydrothermal route with DL-malic acid and ethylenediamine as raw materials and further explored the impacts of the amount of reactants, the reaction time, and temperature upon the optical properties of prepared CDs, to select the optimal preparation parameters. The optical properties, surface groups, and element components of the prepared CDs have been systematically studied by UV-Vis absorption spectra, fluorescence spectra, FTIR, EDS, and XPS. The prepared CDs have been applied to realize the fingerprint development, fluorescence ink, and, specifically, the detection of Fe2+ through an inner filter effect with its detection range at .
研究领域:
材料科学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）