COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2023
·
IF 5.2
Shi Zhoutong,Li Jun,Wei Gang,Cheng Biao
In organisms, the over ingesting of Cu 2+ and Zn 2+ will result in the poisoning of living cells, which severely damages the human body. Meanwhile, endogenous Histidine (His) can cause metabolic disorders like Histidinemia (His). Therefore, it is a pressing matter to design an effective method that accurately detects the amount of Cu 2+ , Zn 2+ , and His. In our study, through the nucleophilic addition and substitution reactions synthesis methods, a novel multifunctional probe, Rhodamine derivatives with vinyl functional groups at the end (RHDA) was designed and synthesized. The probe RHDA can recognize Cu 2+ and Zn 2+ separately, and the experiment further substantiates that the RHDA probe can demonstrate various turn-on colorimetric and fluorescent signals, i.e ., selectively detecting Cu 2+ in DMSO/H 2 O solution (v/v=4/6) with the “naked-eye” show evident and significant color change from colorless to pink and Zn 2+ with strong green fluorescence in DMF/H 2 O (v/v=1/1). Under optimal conditions, they could be identified under the low detection limit of 0.10 μmol·L −1 and 16.0 nmol·L −1 for Cu 2+ and Zn 2+ , respectively. Furthermore, Histidine (His) was recognized by the formed RHDA-Zn 2+ with a fluorescence “turn-off” range of 0.03–10.0 μM and a detection limit of 230.0 nmol L −1 . This article performed an extensive study of the sensing mechanisms of RHDA toward two metal ions and the ensemble RHDA-Zn 2+ toward His. The practical application experiments in DMF/H 2 O (v/v=1/1) further established that the chemosensor RHDA exhibits good cell permeability and could be employed to monitor free Zn 2+ . Finally, the complex RHDA-Zn 2+ could be applied to assay His in the living HeLa cells and zebrafishes, respectively.
研究领域:
生物化学
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分析化学
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医学
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产品关联
DOI
高影响因子
DYES AND PIGMENTS
·
2020
·
IF 4.613
Jing-Yang Ni,Xiao-Feng Weng,Ru Sun,Yu-Jie Xu,Jian-Feng Ge
1,8-Naphthalimide is widely applied in the construction of probes because it is easy to be modified. However, 1,8-naphthalimide has been reported rarely in the application of organelle labelling especially lipid droplets (LDs). Herein, three types of amine compounds, aliphatic amine ( a , b ), aromatic amine ( c , d ) and heterocyclic amine ( e , f ), were chosen to construct fluorescent dyes ( 1a - 1f ) with 4-bromo- N -butylnaphthalimide ( 1 ) by Ullmann reaction. The maximum emission wavelength ranged from 490 nm to 550 nm. What's more, 1a-1e showed high fluorescence quantum yields (0.54–0.93) in chloroform and 1e-1f had excellent photostability (>92%). In addition, the cell imaging experiment showed that they could label lipid droplets for HeLa cancer cells and L929 normal cells with extremely low toxicity. This work realizes the classical fluorophore 1,8-naphthalimide to synthesize a series of LDs markers without complex synthesis steps.
研究领域:
化学
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生物学
·
材料科学
✓ 已验证
DOI
高影响因子
Food Science & Nutrition
·
2024
·
IF 3.5
Peiyu Xue,Hongmei Zhao,Xinyong You,Weiming Yue
Lactoferrin and polyphenols are common natural functional compounds. Their interactions and the consequential alterations in functional activity have received widespread attention. The work aimed to investigate the interaction between lactoferrin and kaempferol, as well as evaluate the in vitro anticancer properties of the lactoferrin–kaempferol complex. The results of the spectra experiments revealed that lactoferrin and kaempferol are capable of forming complexes to quench the endogenous fluorescence of lactoferrin. Further insight into the binding mechanism was gained through molecular docking and molecular dynamics simulations. These analyses suggest that both hydrophobic interactions and hydrogen bonding are essential factors in the interaction between lactoferrin and kaempferol. Furthermore, the MTT assay and apoptosis by flow cytometry were conducted, revealing a synergistic effect of kaempferol and lactoferrin on the inhibition of HeLa cell proliferation. The findings from this investigation could improve our understanding of lactoferrin's interaction with polyphenols and the role of the lactoferrin–kaempferol complex in inhibiting cancer cell proliferation.
研究领域:
药理学
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癌症研究
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分子生物学
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生物化学
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2013
·Vol. 1
·期 20
·2582-2589
·
IF 0
Mei-Ling Chen,Li-Ming Shen,Shuai Chen,Hui Wang,Xu-Wei Chen,Jian-Hua Wang
β-FeOOH has shown promise as a new contrast agent in magnetic resonance imaging (MRI), however, sensitive and accurate MR imaging is largely limited by its low transverse relaxivity (r2). Herein, for the first time we report in situ growth of β-FeOOH nanorods onto PEGylated graphene oxide (GO) sheets to produce a nanocomposite, e.g., GO-PEG–β-FeOOH. This nanocomposite exhibits a record ultra-high transverse relaxivity (r2) value of 303.81 mM−1 s−1, that is, >60 times higher than those achieved by hitherto reported β-FeOOH based MRI contrast agents. This well facilitates its practical use as a contrast agent for in vivo MR imaging. PEG on the surface of the GO nanocomposite improved the colloidal stability in aqueous medium. In addition, in vitro cell viability tests demonstrated that GO-PEG–β-FeOOH has minimal cellular toxicity. GO-PEG–β-FeOOH has been used for loading doxorubicin hydrochloride (DOX) with a capacity of 1.35 mg mg−1, which exhibits high efficiency in Hela cell apoptosis. These results indicated that GO-PEG–β-FeOOH provides an effective alternative to the existing nanoparticle-based contrast agents for non-invasive in vivo MR imaging and cancer therapy.
研究领域:
纳米技术
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材料科学
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生物医学工程
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癌症研究
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医学影像
✓ 已验证
DOI
PubMed
ACS Omega
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2018
·
IF 0
Cunji Gao,Xiaohua Liang,Zhengxi Guo,Bang-Ping Jiang,Xiaoming Liu,Xing-Can Shen
In this study, we have evaluated a water-soluble, nontarget reagent and a carrier-free diiron hexacarbonyl complex, [Fe2{μ-SCH2CH(OH)CH2(OH)}2(CO)6] (TG-FeCORM), that can induce the site-specific release of carbon monoxide (CO) in cancer cells triggered by endogenous glutathione (GSH). The releasing rate of CO was dependent on the amount of endogenous GSH. Being the amount of endogenous GSH higher in cancer cells than in normal cells, the CO-releasing rate resulted faster in cancer cells. Moreover, the anti-inflammatory properties related to the intracellular CO release of TG-FeCORM were also confirmed in the living HeLa cells.
研究领域:
药理学
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肿瘤学
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化学
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细胞生物学
✓ 已验证
产品关联
DOI
PubMed
CHINESE JOURNAL OF CHEMISTRY
·
2016
·Vol. 34
·期 6
·609-616
·
IF 1.872
Wei Zhang,Wenjun Zhu,Ruiyu He,Shuo Fang,Yemin Zhang,Chen Yao,Muhammad Ismail,Xinsong Li
Chlorambucil is a classic nitrogen mustard drug that has been used in the treatment of cancers. It may induce neutropenia, thrombocytopenia and other side effects because of its short lifetime and off-target effect. In this report, chlorambucil-tetrapeptide (AAAK, A 3 K) conjugate vesicles were developed to improve the stability and bioactivity of chlorambucil. First of all, chlorambucil-A 3 K conjugate was synthesized by solid phase synthesis strategy. Secondly, the chlorambucil-A 3 K conjugate was assembled and characterized by critical aggregation concentration, circular dichroism, dynamic light scattering and transmission electron microscopy. The results indicated that the chlorambucil-A 3 K conjugate can be assembled to form spherical vesicles with an average diameter of 390.5 nm, and high drug loading about 47.1% is reached. Surprisingly, the preliminary biological evaluation of the chlorambucil-A 3 K conjugate vesicles revealed the best in vitro anticancer activity against HeLa, HepG-2 and MCF-7 cell lines compared with chlorambucil and chlorambucil-A 3 K conjugate free drugs. Furthermore, conjugate vesicles showed excellent in vivo antitumoral activity. It can be partly attributed to their vesicular structure which isolates chlorambucil active moiety from aqueous solution to retard degradation before killing cancer cells. Therefore, chlorambucil-peptide (A 3 K) conjugate vesicles may be an alternative delivery system of chlorambucil.
研究领域:
药学
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癌症研究
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纳米技术
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药物递送系统
✓ 已验证
产品关联
DOI
高影响因子
DRUG METABOLISM AND DISPOSITION
·
2016
·Vol. 44
·期 4
·485-488
·
IF 3.21
Shuai Wang,Feng Li,Enxi Quan,Dong Dong,Baojian Wu
Resveratrol undergoes extensive metabolism to form biologically active glucuronides in humans. However, the transport mechanisms for resveratrol glucuronides are not fully established. Here, we aimed to characterize the efflux transport of resveratrol glucuronides using UGT1A1-overexpressing HeLa cells (HeLa1A1 cells), and to determine the contribution of multidrug resistance–associated protein (MRP) 4 to cellular excretion of the glucuronides. Two glucuronide isomers [i.e., resveratrol 3-O-glucuronide (R3G) and resveratrol 4′-O-glucuronide (R4′G)] were excreted into the extracellular compartment after incubation of resveratrol (1–100 μM) with HeLa1A1 cells. The excretion rate was linearly related to the level of intracellular glucuronide, indicating that glucuronide efflux was a nonsaturable process. MK-571 (a dual inhibitor of UGT1A1 and MRPs) significantly decreased the excretion rates of R3G and R4′G while increasing their intracellular levels. Likewise, short-hairpin RNA (shRNA)–mediated silencing of MRP4 caused a significant reduction in glucuronide excretion but an elevation in glucuronide accumulation. Furthermore, β-glucuronidase expressed in the cells catalyzed the hydrolysis of the glucuronides back to the parent compound. A cellular pharmacokinetic model integrating resveratrol transport/metabolism with glucuronide hydrolysis/excretion was well fitted to the experimental data, allowing derivation of the efflux rate constant values in the absence or presence of shRNA targeting MRP4. It was found that a large percentage of glucuronide excretion (43%–46%) was attributed to MRP4. In conclusion, MRP4 participated in cellular excretion of R3G and R4′G. Integration of mechanistic pharmacokinetic modeling with transporter knockdown was a useful method to derive the contribution percentage of an exporter to overall glucuronide excretion.
研究领域:
药理学
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细胞生物学
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药物代谢
✓ 已验证
DOI
PubMed
高影响因子
CURRENT PHARMACEUTICAL DESIGN
·
2025
·Vol. 31
·期 42
·3416-3426
·
IF 2.8
Chenguang Yao,Ting Tan,Jiangning Yan,Zijia Zhao,Romina Onintsoa Diarimalala,Junjie Wang,William Wang,Hanluo Li,Jingbiao Liu,Yanhong Wei,Kanghong Hu
ObjectiveTo develop new Anderson-type polyoxometalates (POMs) with high efficiency and low cytotoxicity, and investigate the effects and mechanisms against lung (A549), cervical (Hela), and breast cancer (MCF7) cell lines.MethodsCytotoxicity assessments on Hela, A549, and MCF-7 tumor cells were tested by MTT assay. Antitumor activities of B1 (vanadium-centered, methyl-modified) and B7 (vanadium-centered, hydroxyl-modified) were detected by apoptosis, scratch, and colony formation assay. The antitumor molecular mechanisms were explored by Western blotting.ResultsThis study synthesized and evaluated twelve Anderson-type compounds which were centered with vanadium, chromium, iron, cobalt, nickel, and copper heteroatoms, modified with methyl and hydroxyl at the side chains. Cytotoxicity assessments revealed that compounds B1 and B7 exhibited superior efficacy, with IC50 values of approximately 7 μmol/L of three cell lines. B1 and B7 inhibited proliferation and migration in these cell lines and induced apoptosis in MCF7 and A549 cells. Mechanistic investigations indicated that B1 induces apoptosis in MCF7 cells by inhibiting the AKT signaling pathway and downregulating the expression of apoptosis-related proteins Bcl-2 and Caspase-9.ConclusionNovel Anderson-type POMs B1 (vanadium-centered, methyl-modified) and B7 (vanadium-centered, hydroxyl-modified) exhibited superior efficacy against tumor cells and induced apoptosis via PI3K/AKT pathway, which provides new theoretical avenues for developing POM-mediated antitumor chemotherapeutic medications.
研究领域:
肿瘤学
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药理学
·
分子生物学
·
无机化学
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转化医学
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细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED CLAY SCIENCE
·
2020
·
IF 4.605
Xiaoyun Mo,Fuling Wu,Binglong Yu,Wenlong Wang,Xiulan Cai
Halloysite nanotubes have been gradually applied in the field of biomedicine, due to their attractive properties including biocompatibility, nontoxicity, hydrophilic and low-cost. Here, polyglycerol (PG) is used to wrap the surface of Hal to yield Hal-PG, so that there are a large number of hydroxyl groups on the surface of Hal-PG. Then, Hal-PG-FA nanocomplexes were prepared by combining folate (FA) with Hal-PG via esterification reaction. Thereafter, doxorubicin (DOX) was loaded on novel targeted anticancer delivery system Hal-PG-FA that can bind specifically to tumor cells that overexpressed the folate receptor. The obtained nanomaterials were fully characterized using FT-IR, TEM , XPS , TGA and DLS techniques. Moreover, the anticancer activity of Hal-PG-FA/DOX was detected by Cell Counting Kit-8 (CCK8) and flow cytometry (FCM). In vitro cytotoxicity assay preliminarily proved that the Hal-PG-FA/DOX had a more significant inhibitory effect than the free DOX. The results of flow cytometry showed that apoptosis rate on HeLa cells was enhanced by Hal-PG-FA/DOX. All these results showed that folate-PG modified halloysite is an excellent nano anticancer drug carrier in cancer therapy.
研究领域:
生物医学工程
·
纳米技术
·
药学
·
肿瘤学
✓ 已验证
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Ning Wang,Yiting Wang,Tingting Guo,Ting Yang,Mingli Chen,Jianhua Wang
A simple one-step hydrothermal green approach was reported for the preparation of carbon dots (CDs) without any further decoration or modification with papaya powder as natural carbon source. In this economical and eco-friendly system, deionized water or 90% ethanol was used as solvent to produce water-soluble or ethanol-soluble CDs, respectively, termed as W-CDs and E-CDs. The quantum yield (QY) for W-CDs was 18.98%, while that for E-CDs was 18.39%. The potentials of the prepared carbon dots toward diverse applications were thoroughly investigated. W-CDs and E-CDs provide promising probes for fluorescence detection of Fe 3+ , offering limits of detection of 0.48 μmol L −1 and 0.29 μmol L −1 , respectively. W-CDs was further demonstrated to be a promising probe for fluorescence sensing of Escherichia coli O157: H7, along with a limit of detection of 9.5×10 4 cfu mL −1 . Meanwhile, both W-CDs and E-CDs exhibit favorable biocompatibility, and demonstrated to be efficient for Hela cell imaging.
研究领域:
材料科学
·
分析化学
·
生物技术
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2015
·
IF 4.152
Hui Wang,Haifeng Wei,Quan Huang,Hongmei Liu,Jingjing Hu,Yiyun Cheng,Jianru Xiao
Cationic dendrimers are associated with relatively low transfection efficacy and high toxicity in gene delivery. Surface modification of these dendrimers with functional ligands may resolve these issues. Here, we proposed a novel strategy to prepare surface-engineered dendrimers with high transfection efficacy and low toxicity on transfected cells. Several nucleobase analogues were modified on cationic dendrimers by a facile method. These nucleobase-modified dendrimers show improved transfection efficacy and reduced cytotoxicity compared to unmodified dendrimers on HEK293 and HeLa cells. Efficacy of the most efficient polymer is comparable to that of commercial transfection reagents such as SuperFect, PolyFect, and Lipofectamine 2000. The improved transfection efficacy of dendrimers after nucleobase modification is probably attributed to easier intracellular DNA unpacking and lower cytotoxicity. The results provide a valuable insight to guide the design of efficient and low cytotoxic gene vectors.
研究领域:
分子生物学
·
基因治疗
·
药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Minghui Zan,Lang Rao,Huiming Huang,Wei Xie,Daoming Zhu,Li Li,Xingwang Qie,Shi-Shang Guo,Xing-Zhong Zhao,Wei Liu,Wen-Fei Dong
Numerous methods including chromatography, chemiluminescence and spectrophotometry have been reported to detect the nitrite ions. Recently, fluorescent carbon quantum dots have drawn more and more attention as effective sensors to small ions or biomolecules. Here, we will present a solvothermal method to prepare phosphorus and nitrogen codoped carbon quantum dots (PNCQDs) with green-emitting (quantum yield 18.9%). The as-prepared carbon quantum dots exhibit good photostability, low-toxicity and favourable biocompatibility. In addition, the PNCQDs show a high selectivity detection of NO 2 − with a linear relationship between 10 nM and 90 nM and a detection limit as low as 3.3 nM. Meanwhile, the PNCQDs as fluorescent nanoprobes for detection of nitrite were efficient applied in HeLa cells imaging and potential applications in biologically related study.
研究领域:
分析化学
·
生物成像
·
纳米技术
·
环境科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）