BIOSENSORS & BIOELECTRONICS
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2017
·
IF 7.78
Wei-Jia Zeng,Ni Liao,Yan-Mei Lei,Jing Zhao,Ya-Qin Chai,Ruo Yuan,Ying Zhuo
In this work, hemin was firstly used as electrochemically regenerable co-reaction accelerator for signal amplification to develop an ultrasensitive aptasensor for Aflatoxin M1 (AFM1) detection. Initially, the perylenetetracarboxylic acid (PTCA) was directly employed as luminophore to construct the ECL sensing nano-platform by combining Au nanoparticles (Au NPs) for immobilizing thiol-terminated hairpin probe (H1). Then with the help of hairpin H2, H3, the AFM1-catalyzed hairpin assembly (CHA) was executed to produce the H1-H3 duplex, which could further initiate the hybridization chain reaction (HCR) to generate dendritic DNA polymers consisting of G-rich sequence for capturing large quantities of hemin on the electrode surface . Herein, hemin as electrochemically regenerable co-reaction accelerator could interact with the co-reactant (S 2 O 8 2− ) to obviously improve the luminous efficiency of the PTCA. Therefore, a strong and stable ECL signal was achieved by the employment of hemin as electrochemically regenerable co-reaction accelerator. The proposed aptasensor determined AFM1 down to 0.09 pg mL −1 within a linear range of 0.4 pg mL −1 to 400 ng mL −1 . With excellent sensitivity and stability, the strategy provided an efficient and simple method for the trace detection of biomolecules in clinical analysis.
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高影响因子
BIOELECTROCHEMISTRY
·
2017
·
IF 3.346
Beibei Wang,Xueping Ji,Jujie Ren,Ruixing Ni,Lin Wang
A simple, ultrasensitive peroxynitrite anion (ONOO − ) electrochemical sensing platform was developed by immobilizing hemin on a density controllable electrochemically reduced graphene oxide-Au nanoparticles (ERGO-AuNPs) nanohybrids . The ERGO-AuNPs in situ nanohybrids were produced onto a glass carbon electrode (GCE) by one-step electrodeposition, the density of which could be easily controlled by electrodeposited time. The morphology of ERGO-AuNPs nanohybrids was characterized by a scanning electron microscope (SEM). The ERGO-AuNPs nanohybrids showed a high electrocatalytic activity for immobilized-hemin, because the nanostructures hybrids could effectively promote electron transfer rate between hemin and the electrode. Due to nanohybrids-enhanced catalytic effect for hemin, they were firstly selected for use as a highly sensitive electrochemical platform for ONOO − detection. The resulted sensor showed a high electrocatalytic activity toward ONOO − oxidation , being free from the electroactive interferents, including nitrite, nitrate, dopamine and uric acid at an applied potential of 0.7 V. The sensor exhibited a high sensitivity of 123.1 nA μM − 1 and a lower detection limit of 0.1 μM, and a wide linear range of 2.4 × 10 − 6 to 5.5 × 10 − 5 M, which could be attributed to the synergy between ERGO and AuNPs in hybrids. The nanohybrids in situ preparation and ONOO − detection methods would be beneficial to developing other sensing interface and have promising applications in biological molecules analysis and clinical diagnostic.
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FOOD CONTROL
·
2024
·
IF 5.6
Xiaowei Chen,Yujun Feng,Yuanyuan Wang,Xin Yang,Xingping Zhang,Hualin Yang,Yu Zhou
With the gradual increase of oxytetracycline (OTC) residue in the environment, it is particularly important to establish a sensitive and efficient method to detect OTC. In this work, a novel colorimetric and fluorescence dual-mode detection method based on hemin/G-quadruplex (hemin/G4) DNAzyme and zeolitic imidazolate framework-8 (ZIF-8) is developed. OTC can compete with G4 to bind hemin to reduce the amount of hemin/G4. Thus, the oxidation of 3, 3′, 5, 5′-tetramethylbenzidine (TMB) to blue color by hemin/G4 is inhibited. In addition, the OTC or OTC-hemin can be aggregated with the help of ZIF-8 to produce strong green fluorescence due to the aggregation-induced emission (AIE) effect. In colorimetric and fluorescence mode, the linear ranges of the new method are 0.375–18 μM and 0–52.5 μM, with a limit of detection (LOD) of 0.116 μM and 0.040 μM, respectively. Besides, this new method has a good selectivity and recovery rate in the detection of real samples. Therefore, the method can be used for the efficient and sensitive detection of OTC.
研究领域:
分析化学
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环境科学
·
生物技术
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高影响因子
JOURNAL OF MOLECULAR STRUCTURE
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2025
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IF 4.7
Lina Yang,Suimin Deng,Hailing Liu,Xiangfeng Wang,Pingye Deng,Yuan Liu,Mengxia Xie
The pollution of water environment resulted from antibiotic abuse and harmful cyanobacteria blooms poses a serious threat to the ecological environment and human health, thus development of effective approaches to degrade and remove these pollutants is crucial to guarantee environmental security. In this work, TiO 2 /Hemin@MAF-7 composites with photo-Fenton-like cascade catalytic degradation activity have been successfully prepared by a self-assembly method. The photocatalysts exhibit high degradation efficiency towards tetracycline antibiotics in water solution under UV light irradiation. Furthermore, the TiO 2 /Hemin@MAF-7 composites have the potential ability to degrade other organic pollutants under the irradiation of visible light. A possible photocatalytic degradation mechanism of the photocatalyst is proposed, which follows a Z-scheme heterojunction type. The introduction of the hemin component into the heterojunction composites significantly promotes the separation of the photo-generated charges on the catalyst, which drives the photo-Fenton-like cascade catalytic cycle degradation reaction. Additionally, the photocatalyst can effectively inhibit the growth of the Microcystis aeruginosa in water environment by damaging the structure of algal cells. This work provides a practical solution for the degradation of antibiotic residues and control of harmful cyanobacteria blooms in wastewaters, and offers a promising strategy for efficiently removing multiple emerging contaminants in water environments.
研究领域:
环境科学
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生物学
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2022
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IF 9.965
Qiao Teng,Haifeng Wu,Hao Sun,Yuanxi Liu,Hui Wang,Zhen-Gang Wang
Reconstruction of enzymatic active site in an artificial system is key to achieving high catalytic efficiency. Herein, we report the self-assembly of the lysine-containing peptides with guanine-rich DNA and hemin to form peroxidase-mimicking active sites and catalytic nanoparticles . The DNA strand self-folds into a G-quadruplex structure that provides a supramolecular scaffold and a potential axial ligand for hemin. The β-sheet forming capability of the lysine-containing peptides is found to affect the catalytic synergy between the G-quadruplex DNA and the peptide. It is hypothesized that the β-sheet formation of the peptides results in the enrichment of the lysine residues , which distribute on the distal side of hemin to promote the formation of Compound I, like distal arginine residue in natural heme pocket. Incorporation of the histidine residues into the lysine-containing peptides further enhanced the hemin activities, indicating the cooperation between the lysine and histidine. Furthermore, the peptide/DNA/hemin complexes can be switched between active and inactive state by reversible formation and deformation of the DNA G-quadruplex, which was attributed to the peptides-promoted conformational changes of the DNA components. This work opens an avenue to mimic the catalytic residues and their spatial distribution in the natural enzymes, and shed light on the design of the smart biocatalysts that can respond to the environmental stimuli.
研究领域:
生物化学
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分子生物学
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纳米生物学
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高影响因子
Applied Biological Chemistry
·
2026
·Vol. 69
·期 1
·42
·
IF 4.2
Gao Xinyue,You Long,Qi Luyao,Jin Lei,Zhang Jianmei,Liu Yanan,Niu Zhiqiang
As a disease of high mortality rate, acute lung injury (ALI) has been getting increased attention. Heme oxygenase-1 (HO-1), which plays an essential role in alleviating the pathological symptoms of ALI. There have few studies noted that the improvement effect of HO-1 on ALI may associated to NOD-like receptor protein 3 (NLRP3) inflammasome. However, the roles of HO-1 in the process of NLRP3 inflammasome activation is remains unknown. This study investigated the mechanism that HO-1 blocking NLRP3 inflammasome in ALI. The sepsis model induced by LPS was used in C57BL6 mice, LPS triggered obviously pulmonary damage and edema formation, increased the expression of NLRP3 inflammasome component proteins and IL-1β, but those negative effects were reversed by the pretreatment of hemin. NOD-like receptor signaling pathway was down-regulated after hemin treatment through RNA-Seq. Meanwhile, hemin pretreatment exerted significantly inhibit effect on the activation of NLRP3 inflammasome, and the component proteins of NLRP3 inflammasome were not influenced in immortalization of bone marrow-derived macrophages (iBMDMs). Moreover, the immunoprecipitation and immunofluorescence experiments were indicated that HO-1 could bind to NLRP3, silencing HO-1 prevented the block effect of hemin on NLRP3 inflammasome activation. Our study suggested that hemin-inhibited NLRP3 inflammasome activation involved HO-1 bind to NLRP3 and alleviated ALI mice induced by LPS.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Guannan Le,Xiaoyu Ma,Ling Cai,Wei Wei,Jinhuan Li,Jin Chen
The effectiveness of antibiotic treatment for bacterial-infected wounds has been greatly hampered by the increasing antimicrobial resistance (AMR) caused by improper antibiotic use. It is thereby demanding for the deployment of antibiotic-free and on-demand therapeutic strategies, such as chemodynamic therapy (CDT) and photothermal therapy (PTT). Here, via a desolvation method, L ysozyme- H emin nanocomposite was firstly prepared and further decorated by S ilver nanoparticles to form LHS NPs, which was then incorporated into a thermosensitive hydrogel. The LHS NPs demonstrated remarkable peroxidase-like activity and photothermal effect, with a specific activity of 2.59 U/mg and a photothermal conversion efficiency of 53.89 %. Consequently, it was found that the formed LHS NPs exhibited superior antibacterial activity against both Gram-negative and Gram-positive bacteria without the using of antibiotics. Moreover, through the optimization of nanozymes and hydrogels, the obtained nanozyme-hydrogel composite could adhere to wound sites of various shapes within 2 min upon NIR irradiation, enabling the on-demand wound treatment. Notably, in a methicillin-resistant Staphylococcus aureus ( MRSA )-infected wound model, the nanocomposite demonstrated excellent antibacterial activity and greatly sped up wound healing. Therefore, the LHS nanoparticles-loaded thermosensitive hydrogel as an integrated CDT/PTT therapeutic nanoplatform holds enormous potential in combating drug-resistant bacteria towards the complex infected wounds management.
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BIOSENSORS & BIOELECTRONICS
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2020
·
IF 10.257
Guojuan Zhang,Zhiguang Liu,Lifang Fan,Yujie Han,Yujing Guo
A dual signal and label-free electrochemical aptasensor for mucin 1 was constructed based on hemin/ [email protected] nanocomposite ( [email protected] ). Hemin attached on the graphene surface not only improves the solubility of graphene and acts as an in-situ electrochemical probe but also exhibits excellent peroxidase-like properties to electrocatalyze the reduction of H 2 O 2 . PdPtNPs also show outstanding catalytic capacity to the reduction of H 2 O 2 and provide numerous binding sites for loading dDNA (mucin 1 aptamer and cDNA) to form the sensing interface. In the presence of mucin 1, due to the specific affinity between aptamer and mucin 1, double helix would be induced dissociation and the aptamer would be pulled off from the electrode. As a result, the electrochemical signals of hemin and H 2 O 2 were recovered. Based on these properties, the label-free and sensitive dual signal electrochemical biosensor for mucin 1 detection has been developed. The one is differential pulse voltammetry (DPV) signal of hemin and the other is chronoamperometry signal arisen from the catalytic reduction of H 2 O 2 . The linear ranges for mucin 1 were 8.0 pg mL −1 to 80 ng mL −1 and 0.8 pg mL −1 to 80 ng mL −1 with the limit of detection 2.5 pg mL −1 and 0.25 pg mL −1 by DPV and chronoamperometry, respectively. The recovery of mucin 1 in human blood serum samples was from 95.0% to 104.2%. The detection platform does not need signal labeling which greatly reduced the sophisticated and expensive procedures. The aptasensor provide a promising strategy for the determination of mucin 1 in clinical diagnostics.
研究领域:
生物传感器
·
分析化学
·
癌症诊断
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电化学
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2022
·
IF 9.136
Peide An,Defeng Hu,Yide Han,Hao Meng,Xia Zhang
On accounting of the structure advantages of MOFs in the enzyme mimics and enzyme immobilization, in this work, the Hemin@PCN-222 (Mn) is constructed through an in-situ hydrothermal route by covalently bonded the –COOH of Hemin with the Zr(IV) of PCN-222 (Mn). With the help of the synergistic effects, the resulting Hemin@PCN-222 (Mn) represents excellent peroxidase-like activities in comparison with the single Hemin and PCN-222 (Mn) with a special enzyme activity of 6.71 U mg −1 . The Hemin@PCN-222 (Mn) was then employed as a carrier to immobilize the glucose oxidase (GOx), the immobilization conditions were optimized. Based on that, the optimized GOx@hemin@PCN-222 (Mn) was applied in the following cascade reaction: the glucose oxidation in the presence of oxygen catalyzed by the immobilized GOx firstly, and then the produced H 2 O 2 worked as an oxidant to oxidize ABTS. The quantity of produced ABTS + was monitored to assess the catalytic efficiency, and the contrast experiments were conducted over the mixture of the natural GOx and HRP. The above cascade process could also be used to detect the glucose, and the detection limit is determined. The high efficiency and the stability of the resulting GOx@hemin@PCN-222 (Mn) highlights the prospect applications of the MOFs-based enzyme mimics in the bio-catalysis and biosensing.
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Journal of Functional Biomaterials
·
2022
·Vol. 14
·期 1
·15
·
IF 4.901
Lixin Long,Yang Song,Xiaoyi Tian,Lanyue Cui,Chengbao Liu,Shuoqi Li,Yu Wang,Rongchang Zeng
Photoactivated sterilization has received more attention in dealing with implant-associated infections due to its advantages of rapid and effective bacteriostasis and broad-spectrum antibacterial activity. Herein, a micro-arc oxidation (MAO)/polymethyltrimethoxysilane (PMTMS)@hemin-induced calcium-bearing phosphate microsphere (Hemin-CaP) coating was prepared on pure magnesium (Mg) via MAO processing and dipping treatments. The morphology and composition of the coating were characterized via scanning electron microscopy, Fourier transform infrared spectrometer, X-ray diffractometer and X-ray photoelectron spectrometer. Corrosion behavior was evaluated through electrochemical and hydrogen evolution tests. The release of Fe3+ions at different immersion times was measured with an atomic absorption spectrophotometer. Antibacterial performance and cytotoxicity were assessed using the spread plate method, MTT assay and live/dead staining experiment. The results showed that the corrosion current density of the MAO/PMTMS@(Hemin-CaP) coating (4.41 × 10−8A·cm−2) was decreased by two orders of magnitude compared to that of pure Mg (3.12 × 10−6A·cm−2). Photoactivated antibacterial efficiencies of the Hemin-CaP microspheres and MAO/PMTMS@(Hemin-CaP) coating reached about 99% and 92%, respectively, which we attributed to the photothermal and photodynamic properties of hemin with a porphyrin ring. Moreover, based on the release of Fe3+ions, the MC3T3-E1 pre-osteoblasts’ viability reached up to 125% after a 72 h culture, indicating a positive effect of the coating in promoting cell growth. Thus, this novel composite coating holds a promising application as bone implants.Keywords:magnesium;degradable biomaterial;antibacterial coating;biocompatibility;hemin
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ANALYTICAL CHEMISTRY
·
2014
·
IF 5.825
Chenchen Ge,Quan Luo,Dou Wang,Shiming Zhao,Xiaoling Liang,Luxin Yu,Xuerong Xing,Lingwen Zeng
G-quadruplex-forming sequence can be formed through a copper(I) ion (Cu+)-catalyzed click chemistry between azide- and alkyne-modified short G-rich sequences in aqueous solution, eliminating immobilization and washing steps of conventional assays. The source for Cu+ was generated from the reduction of Cu2+ with the reductant of sodium ascorbate. In the presence of hemin and K+, the self-assembly of hemin/G-quadruplex structure has the activity of horseradish peroxidase (HRP), which can catalyze its colorless substrate tetrazmethyl benzidine (TMB) into a colored product. Hence, the concentration of Cu2+ can be evaluated visually for qualitative analysis according to the color change of the solution, and the optical density (OD) value of the resulting solution at 450 nm was also recorded using a microplate reader for quantitative analysis.
研究领域:
生物化学
·
分析化学
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Materials Research Express
·
2014
·Vol. 1
·期 4
·045601
·
IF 0
Wenjing Huang,Qingli Hao,Wu Lei,Lihua Wu,Xifeng Xia
An efficient and eco-friendly microwave-assistant method is developed to synthesize a ternary composite of polypyrrole-hemin-reduced graphene oxide (PPY-He-RGO). The polymerization of the pyrrole monomer and the reduction of graphene oxide are performed simply by microwave heating without using a strong reducing or oxidizing agent in an isopropanol/H2O mixed medium. Hemin molecules are immobilized on reduced graphene oxide (RGO) sheets and can still retain high electrocatalytic activity toward the reduction of H2O2 in the final composite. The conducting RGO and polypyrrole with a well-controlled nanostructure provide a highly conductive network to the ternary composite, which can promote the electron transfer between hemin, analytes and electrodes, leading to an improved electrocatalytic activity. The PPY-He-RGO can act as a third-generation mediator and mimic enzyme for the fabrication of a hydrogen peroxide biosensor. The as-prepared PPY-He-RGO electrode exhibits a high sensitivity to H2O2 with a low detection limit of 0.13 μm. The efficient microwave heating provides an opportunity for large-scale production of PPY-He-RGO ternary nanocomposites as a kind of mimic enzyme for biosensors.
研究领域:
材料科学
·
生物传感器
·
电化学
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危险品化学品经营许可证（带存储）