ADSORPTION SCIENCE & TECHNOLOGY
·
2021
·
IF 4.232
Yuan Yang,Xin Liu,Lin Luo,Wenjing Wei,Qiang Wang,Jiachao Zhang
The increasing usage of zinc oxide nanoparticles (ZnONPs) inevitably leads to their release into the environment. To understand their fate and toxicity in water systems, a reliable method for the quantitative analysis of ZnONPs in environmental waters is urgently needed to be established. In this study, a quantitative analytical method of ZnONPs in environmental waters was developed by cloud point extraction (CPE) combined inductively coupled plasma mass spectrometry (ICP-MS). To obtain high recoveries of ZnONPs, the CPE parameters including pH, surfactant concentration, salt concentration, bath temperature, and time were optimized. The results demonstrated that the addition of β-mercaptoethylamine could significantly reduce the interference of Zn2+ on the extraction of ZnONPs, while the CPE approach was not affected significantly by the typical environmental inorganic ion and ENMs (such as Au, TiO2, and Al2O3). The extraction method of ZnONPs with different diameters was also assessed, and satisfactory extraction efficiency was obtained. The results of ZnONP concentration in collected environmental water were in the range of - μg/L. And the recoveries of ZnONPs in different environmental waters were - at low concentration spiked levels (12.57-54.68 μg/L), demonstrating that it is efficient to extract trace ZnONPs from real environmental waters. This established method offered a reliable method for the quantitative determination of ZnONPs in environmental waters, which could further promote the study of the environmental behavior, fate, and toxicity of ZnONPs in an aqueous environment.
研究领域:
环境科学
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分析化学
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纳米技术
·
水质
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DOI
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2024
·
IF 2.6
Xiaoting Li,Xin Li,Jiayi Chen,Zuomin Tan,Chuan Wang
Bacteremia , as a serious infectious disease, has an increasing incidence and a high mortality rate . Early diagnosis and early treatment are crucial for improving the cure rate. In this work, we proposed an inductively coupled plasma mass spectrometry (ICP-MS)-based detection method combined with gold nanoparticle (Au NP) and silver nanoparticle (Ag NP) labeling for the simultaneous detection of Salmonella and Escherichia coli ( E. coli O157:H7) in human blood samples. Salmonella and E. coli O157:H7 were captured by magnetic beads coupled with anti-8G3 and anti-7C2, and then specifically labeled by Au NP-anti-5H12 and Ag NP-anti-8B1 respectively, which were used as signal probes for ICP-MS detection. Under the optimal experimental conditions, the limits of detection of 164 CFU mL −1 for Salmonella , 220 CFU mL −1 for E. coli O157:H7 and the linear ranges of 400–80,000 CFU mL −1 Salmonella , 400–60,000 CFU mL −1 E . coli O157:H7 were obtained. The proposed method can realize the simultaneous detection of two types of pathogenic bacteria in human whole blood in 3.5 h, showing great potential for the rapid diagnosis of bacteremia in clinic.
研究领域:
医学诊断
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纳米技术
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微生物学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Jie Zhang,Yuheng Lu,Wei Gao,Peng Yang,Nansheng Cheng,Yanwen Jin,Junbo Chen
Ochratoxin A (OTA) is usually found in the contaminated cereal food that its trace concentration would cause severe carcinogenicity and neurotoxicity. Herein, a recognition-triggered structure switching-based element spectrometric assay was proposed for ultrasensitive and highly specific detection of OTA. OTA aptamer was first adapted into a primer for the formation of a locked hairpin (LHP), and followed by attachment on the surface of magnetic beads (MBs). In the presence of OTA, the binding event between OTA molecule and its aptamer induced the liberation of the primer for that is otherwise unable to spontaneously structure-switching. For the rolling circle amplification (RCA), thereby a dumbbell structure was designed as the template (DT). The hybridization of the dumbbell template with free primer initiates the rolling circle amplification along with the production of repeated sequences. To obtain the signal output, the DNA-AuNP tag was prepared which contained the complementary sequences with RCA products. Thus, each tag can hybridize with the repeated sequences derived from the RCA, enabling the formation of detectable signal by the following ICP-MS measurement. Such design can translate a single recognition event into numerous AuNPs, enabling amplified detection of OTA. Taking advantage of this principle, the OTA in real sample (AFP, PSA) can be quantified at femtomolar concentration and it can be expanded into application in food safety inspection.
研究领域:
食品安全
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分析化学
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毒理学
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY
·
2026
·
IF 3.5
Shuang Qin,Di Wu,Chu-Ting Yang,Feng-Cheng Wu,Ning Wang
Accurately identifying plutonium (Pu) valence states is critical to the safety and efficiency of nuclear fuel reprocessing. In this study, an analytical method for Pu valence state speciation was developed based on capillary electrophoresis hyphenated to inductively coupled plasma mass spectrometry (CE-ICP-MS), enabling effective separation and quantification of Pu(III), Pu(IV), and Pu(VI). Optimal separation conditions were established using surrogate elements with stable oxidation states and 2-hydroxyisobutyric acid (α-HIBA) as the background electrolyte (BGE). Under optimized conditions, the migration order of Pu valence states was determined as Pu(III) > Pu(VI) > Pu(IV), with a relative standard deviation (RSD) of migration time < 1.5%, indicating excellent analytical repeatability. The method was successfully applied to monitor the reduction of Pu(VI) by zero-valent iron (Fe 0 ), elucidating the valence state transformation kinetics (Pu(VI) → unstable Pu(IV) intermediate → Pu(III)). Notably, the method exhibited robust anti-interference capability in high uranium (U) matrices, maintaining baseline separation of Pu valence states even at a U/Pu ratio of 100. This study provides a novel, reliable approach for Pu speciation analysis in complex nuclear matrices, with significant implications for process control and Pu recovery in nuclear fuel reprocessing.
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产品关联
DOI
高影响因子
GEOSTANDARDS AND GEOANALYTICAL RESEARCH
·
2018
·Vol. 43
·期 1
·93-111
·
IF 3.929
Shenyang Y. Zhang,Hongluo L. Zhang,Zhenhui Hou,Dmitri A. Ionov,Fang Huang
A rapid sample preparation procedure is described to determine trace element compositions of peridotites using LA-ICP-MS. Peridotite powders were fused with albite in a molybdenum–graphite assembly to obtain homogeneous glasses. Best conditions for the fusion procedure (heating at 1500–1550 °C for 10–15 min with a sample-to-flux ratio of 1:2) were constrained with melting experiments on two USGS reference materials, PCC-1 and DTS-2B. Mass fractions of first series transition elements, Ba and Pb, in quenched glasses of PCC-1 and DTS-2B are consistent with published data within 10% RSD. Three spinel peridotite xenoliths from eastern China were analysed following both our method and conventional solution ICP-MS. Compared with solution ICP-MS, the relative deviations of our method for most elements were within 10%, while for the REE, Ta, Pb, Th and U, the relative deviations were within 20%. In particular, volatile elements (e.g., Pb and Zn) are retained in the glass. Compared with conventional wet chemistry digestion, our method is faster. Additional advantages are complete sample fusion, especially useful for samples with acid-resistant minerals (spinel and rutile), and long-term conservation of glasses allowing unlimited repeated measurements with microbeam techniques. The same approach can be used for analyses of other mantle rocks, such as eclogites and pyroxenites.
研究领域:
地球化学
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矿物学
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DOI
高影响因子
TALANTA
·
2019
·
IF 4.916
Xiaolan Liu,Man He,Beibei Chen,Bin Hu
Increasing use of TiO 2 nanoparticles (NPs) leads to an accumulation in environment and a risk to human. In this work, an on-line monolithic capillary microextraction (CME)-inductively coupled plasma mass spectrometry (ICP-MS) method was developed for sensitive determination of TiO 2 NPs in environmental water samples. With the presence of citric acid , TiO 2 NPs can be selectively adsorbed on the poly(acrylamide)-vinylpyridine-methylene bis-(acrylamide) (poly(AA-VP-Bis)) monolithic column at pH 1, whereas Ti ions cannot be adsorbed. Factors affecting the extraction efficiency such as sample pH, concentration of citric acid and elution conditions were carefully investigated. Under the optimum conditions, a detection limit of 0.63 μg L −1 for TiO 2 NPs and a sample throughput of 6 h −1 were achieved. The method can be applied to the determination of TiO 2 NPs in the size range of 5–100 nm. The developed method was then applied to the analysis of TiO 2 NPs in the East Lake water and Yangtze River water, and recoveries of 84–109% were obtained for spiked samples. Compared with other reported methods for the determination of metal oxide NPs, the developed on-line monolithic CME-ICP-MS has the merits of simple operation, high throughput and no need for digestion before ICP-MS detection.
研究领域:
分析化学
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Zewei Cui,Man He,Beibei Chen,Bin Hu
Due to the enormous interest in plants related to bioscience, environmental and toxicological research, analytical methods are expected with the ability of getting information on elemental transfer, distribution and contents in plants. In this work, a mixture of gelatin (GA) and hydroxypropyl methyl cellulose (HPMC) was prepared to simulate plant matrix, a method based on laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) with matrix-matching external calibration was proposed for direct quantification of multiple elements in plants. The composition of GA&HPMC substrate was optimized, such as the concentration of spiked nitric acid , the mass fraction of both GA and HPMC in the substrate and the mass ratio of GA: HPMC. After spiking elemental solution, coating the mixture onto a glass slide and drying overnight at room temperature , GA&HPMC substrate was obtained. The substrate obtained with GA: HPMC of 8: 2 was used to fabricate the standard series, which exhibited good elemental homogeneity and similar elemental signal intensities in LA-ICP-MS detection to that obtained for plant Certified Reference Material (CRM). CRMs of different plants including Citrus leaf (GBW10019), Tea (GBW07605), Beans (GBW10021) and Scallions (GBW10049) were further pressed into pellets and subjected to the proposed method, and the quantification accuracy was demonstrated. The limits of detections of this method were found to be 0.003 (Ce)-104 (Ca) μg g −1 , with a wide linear range (0.01–10000 μg g −1 ) for 17 target elements. The application potential of the method was further demonstrated by performing elemental imaging in Trigonotis peduncularis leaves. Rapid in-situ quantitative imaging of Zn, Cu, Sr and Mn was achieved, and the elemental quantitative distributions were discussed. The constructed substrate helped direct elemental quantification in plants. It provided a powerful and efficient tool for the investigation of the distribution and transfer of elements in plants, favoring further exploration of elemental bioavailability , transport and toxicity mechanisms.
研究领域:
植物学
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环境科学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2025
·
IF 6.1
Jiawei Geng,Lei Zhao,Qiang Wei,James C. Hower,Shifeng Dai
Coal fly ash is a potential source of critical metals, and accurate trace element analysis is essential for evaluating its resource value and environmental impact. Inductively Coupled Plasma - Mass Spectrometry (ICP-MS) is widely used due to its low detection limits and its capability of multi-element determination. In this study, a mixture of acids (5 mL HF + 2 mL HNO 3 for each 50 mg sample) was used for microwave-assisted digestion of fly ashes from eight Chinese coal-fired power plants and NIST Standard Reference Materials (SRMs) 1633b and 1633c.For low-alumina samples and SRM 1633c, ICP-MS results were consistent with those from X-ray fluorescence spectroscopy (XRF). However, for alumina-rich fly ashes, ICP-MS significantly underestimated most elements. In the worst case, the ICP-MS/XRF ratios for Sc, Ga, Rb, Sr, Y, Ba, La, and Ce in a single sample ranged from 0.37 to 0.68. XRD and EPMA analyses revealed that the formation of AlF 3 during digestion caused co-precipitation and trace element loss. To address this issue, a digestion method using ultrafine grinding (<23 μm) and lower test portion mass (<20 mg) was developed. This modification effectively suppressed AlF 3 formation by lowering the aluminum concentration in solution, resulting in improved ICP-MS/XRF ratios (>0.84) for alumina-rich samples. The method was further validated with additional high-alumina samples and SRMs 1633b and 1633c. This study proposes a simple yet effective pretreatment method to enhance the reliability of ICP-MS results for critical metal determination, which can serve as a valuable reference for elemental analysis of alumina-rich geological and environmental samples.
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产品关联
DOI
PubMed
高影响因子
Frontiers in Nutrition
·
2026
·
IF 5.5
Yao Sun,Wei Zheng,Xianling Yang,Jiayu Deng,Qipin Gao,Duoduo Xu,Yang Gao
IntroductionGrifola frondosa is an edible and medicinal mushroom rich in bioactive polysaccharides, but the relationships among molecular-weight distribution, compositional and linkage features, biological activity, and in vivo distribution remain incompletely characterized.MethodsPolysaccharides from G. frondosa were extracted, purified, and separated into three molecular-weight fractions: PP-1 (>10 kDa), PP-2 (1-10 kDa), and PP-3 (< 1 kDa). The fractions were characterized by total sugar, acidic sugar and protein contents, monosaccharide composition, apparent molecular-weight distribution, and methylation analysis. Their preliminary bioactivities were evaluated using S180, HepG2, and RAW 264.7 cell-based assays. PP-3 was subsequently labeled with fluorescein isothiocyanate followed by iodination, and iodine content was quantified using inductively coupled plasma mass spectrometry (ICP-MS) to evaluate its preliminary in vivo distribution.ResultsAmong the three fractions, PP-3 showed the highest sugar content and a relatively simple detected monosaccharide profile under the present Glc/Gal-standard-based analysis, with methylation analysis suggesting abundant terminal Glcp residues and limited internal glucose linkages. PP-3 exhibited the strongest preliminary in vitro antiproliferative activity against S180 sarcoma and HepG2 hepatocellular carcinoma cells under the present screening conditions. It also enhanced RAW 264.7 macrophage proliferation and increased nitric oxide, TNF-α, IL-1β, and IL-6 production. The ICP-MS method showed good linearity, precision, stability, reproducibility, and recovery. After oral administration in rats, iodine-labeled PP-3-derived material showed limited systemic absorption, with the highest blood absorption rate observed at 2 h, and iodine signal was mainly detected in the liver and kidney.DiscussionBecause ICP-MS detects iodine rather than the intact carbohydrate backbone, the biodistribution findings should be interpreted as preliminary tracer-based distribution data for iodine-labeled PP-3-derived material rather than a full pharmacokinetic profile of the native PP-3 fraction. Overall, this study identifies PP-3 as a low-molecular-weight carbohydrate/oligosaccharide-rich fraction with the strongest preliminary in vitro bioactivity among the tested G. frondosa fractions and provides an ICP-MS-based strategy for tracing iodine-labeled PP-3-derived material, supporting further investigation of mushroom-derived polysaccharide-derived fractions for functional-food and natural-product applications.
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2019
·
IF 3.206
Lanyun Fang,Yumei Zhang,Beibei Lu,Li Wang,Xunping Yao,Ting Ge
The present paper proposed an analytical method for speciation analysis of inorganic antimony in inhalable particulate matter, PM2.5, using high performance liquid chromatography coupling with inductively coupled plasma mass spectrometry (HPLC-ICP-MS). A new extraction method consisted of reducing extraction and oxidizing extraction using EDTA as the key component in the extractant has obtained better extraction yield and preserves stability of both species during the extraction process. Stability of Sb species in EDTA-saturated nitric or tartric acid solution and in aqueous solution were carefully investigated. Using the optimized conditions, the method allows for the determination of Sb(III) and Sb(V) in PM2.5 samples with limits of 0.10 and 0.06 ng m −3 , respectively. The precision, expressed as relative standard deviation (RSD), was always lower than 8.5% and 6.6% for Sb(III) and Sb(V), respectively. The spiked recovery rates of Sb species at three different concentration levels were in the range of 90.0–110.0%. The extraction yield of the sequential extraction process confirmed by analysis of a certified reference material of urban particulate matter, NIST1648a, furnished by National Institute of Standards and Technology was 60%. This method was applied to determine Sb(III) and Sb(V) in PM2.5 samples collected in urban area in Ningbo City, China. The results showed that both Sb(V) and Sb(III) were found in most of the samples analyzed, Sb(V) being the main one, which represents 69–87% of the total Sb extracted in the considered samples, whereas Sb(III) represents the remaining 13–31%.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
·
2017
·Vol. 32
·期 6
·1186-1195
·
IF 3.379
Shan Li,Beibei Chen,Man He,Bin Hu
Protein phosphorylation analysis is important for understanding cell regulations. Mass spectrometry (MS) is a powerful technique for peptide phosphorylation analysis. However, quantification of low abundance phosphoproteins in a complex mixture of proteins is still a challenge. Here we report the development of hollow fiber (HF) supported TiO2 monolithic microextraction combined with capillary high performance liquid chromatography (capHPLC)-inductively coupled plasma-collision reaction cell (ICP-CRC)-MS for the absolute quantification of phosphopeptides. The membrane pores (∼200 nm) of the HF effectively carry more TiO2 on its surface to enhance the adherence of the TiO2 monolith with the HF. By using β-casein as a model phosphorylated protein, we optimized the HF-supported TiO2 monolithic microextraction towards phosphopeptides. Under the optimal conditions, a 100-fold enrichment factor was obtained and the monoliths can be reused 40 times. By monitoring 31P16O with O2 as a reaction gas in the CRC, polyatomic mass interference at m/z 31 is avoided and a low detection limit (2.9 nM) of phosphorus is achieved. The method of HF-supported TiO2 monolithic microextraction-capHPLC-ICP-MS provided the detection limit for phosphopeptides at the nM level. The proposed method was applied to the quantification of phosphopeptides in milk and milk powder samples.
研究领域:
蛋白质组学
·
分析化学
·
生物化学
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产品关联
DOI
高影响因子
TALANTA
·
2022
·
IF 6.556
Wenxin Cui,Zhaoqing Cai,Qing Li,Haiyun Qu,Jiaqian Zheng,Dengguang Yu,Junfeng Chen,Zheng Wang
In this study, a laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) method for in-situ determination of yttrium and trace elements in yttrium-doped barium fluoride (BaF 2 : Y) crystals was proposed. A facile, micro-damage procedure for quantifying the segregation coefficient of doping elements was investigated, and it was found that the actual yttrium doping concentration increases from the seed end to the tail end in BaF 2 : Y crystals. In micro-area analysis, this method has higher mass sensitivity which was applied to quantify the impurity content and distribution during the growth of BaF 2 : Y crystals. Regression coefficient of calibration curve for each element ranged from 0.9918 to 0.9995. Detection limits (DLs) were 0.05, 0.03, 0.01 and 0.01 μg g −1 for Mg, Zn, Sr and Pb, respectively. The accuracy of the proposed method was verified by inductively coupled plasma mass spectrometry/atomic emission spectroscopy (ICP-MS/AES) with wet-chemical pretreatment. The objective of the presented work was to provide a less damaging and more novelty approach for crystal sample analysis.
研究领域:
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）