JBMR Plus
·
2022
·Vol. 7
·期 1
·e10706
·
IF 0
Wenhuan Chai,Weiwei Hao,Jintao Liu,Zhenglin Han,Shiyu Chang,Liben Cheng,Mingxin Sun,Guofang Yan,Zemin Liu,Yin Liu,Guodong Zhang,Li Xing,Hongqian Chen,Peng Liu
The Cre/lox system is a fundamental tool for functional genomic studies, and a number of Cre lines have been generated to target genes of interest spatially and temporally in defined cells or tissues; this approach has greatly expanded our knowledge of gene functions. However, the limitations of this system have recently been recognized, and we must address the challenge of so-called nonspecific/off-target effects when a Cre line is utilized to investigate a gene of interest. For example, cathepsin K (Ctsk) has been used as a specific osteoclast marker, and Cre driven by its promoter is widely utilized for osteoclast investigations. However, Ctsk-Cre expression has recently been identified in other cell types, such as osteocytes, periosteal stem cells, and tenocytes. To better understand Ctsk-Cre expression and ensure appropriate use of this Cre line, we performed a comprehensive analysis of Ctsk-Cre expression at the single-cell level in major organs and tissues using two green fluorescent protein (GFP) reporters (ROSA nT-nG and ROSA tdT) and a tissue clearing technique in young and aging mice. The expression profile was further verified by immunofluorescence staining and droplet digital RT-PCR. The results demonstrate that Ctsk-Cre is expressed not only in osteoclasts but also at various levels in osteoblast lineage cells and other major organs/tissues, particularly in the brain, kidney, pancreas, and blood vessels. Furthermore, Ctsk-Cre expression increases markedly in the bone marrow, skeletal muscle, and intervertebral discs in aging mice. These data will be valuable for accurately interpreting data obtained from in vivo studies using Ctsk-Cre mice to avoid potentially misleading conclusions. © 2022 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research. Graphical We performed a comprehensive analysis of Ctsk-Cre expression at the single-cell level in major organs and tissues using two GFP reporters. These results demonstrate that Ctsk-Cre is expressed not only in osteoclasts but also at various levels in osteoblast lineage cells and other major organs/tissues, particularly in the brain, kidney, pancreas, and blood vessels. These data will be valuable for accurately interpreting data to avoid potentially misleading conclusions.
研究领域:
遗传学
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分子生物学
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细胞生物学
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骨骼与矿物质研究
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产品关联
DOI
PubMed
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2022
·
IF 3.119
Xiao-Feng Guo,Shi-Hua Sang,Chao Ye
The underground brines in the Sichuan basin of China are rich in many valuable components, such as Li+, K+, Sr2+, Br–, and others. In view of comprehensive utilization of the underground brines, the stable solid–liquid equilibria of the quaternary systems LiBr–NaBr–KBr–H2O and LiBr–KBr–SrBr2–H2O at 308.15 K were measured by the isothermal dissolution equilibrium method. The results show that both phase diagrams of the quaternary systems listed above are hydrate-type. Both equilibrium phase diagrams are composed of two invariant points, five univariant curves and four crystallization fields. The equilibrium solid phases in the first quaternary system are LiBr·2H2O, NaBr·2H2O, NaBr, and KBr, respectively, and those in the second quaternary system are LiBr·2H2O, SrBr2·6H2O, SrBr2·2H2O, and KBr. In both phase diagrams, the crystallization field of KBr is the largest, while that of LiBr·2H2O is the smallest. The results indicate that KBr is the easiest to be separated from the saturated solution in the two quaternary systems. Furthermore, the comparison of phase equilibria of the quaternary systems and its ternary subsystems at different temperatures is presented and discussed in detail.
研究领域:
化学工程
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物理化学
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材料科学
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2023
·
IF 12.6
Longshua Qin,Ting Huang,Hanfeng Cui,Mengqing Cheng,Guobing Wei,Fusheng Liao,Wei Xiong,Hedong Jiang,Jing Zhang,Hao Fan
Herein, an fluorescence (FL)−electrochemiluminescence (ECL) dual-mode biosensor is constructed based on the dual-signal “turn-on” strategy of functionalized metal-organic frameworks nanosheets (RuMOFNSs)-tetraferrocene for K-ras gene detection, and the mechanism of bursting through front-line orbital theory is explained for the first time. Amino-functionalized tetraferrocene-labeled probe DNA molecules are linked to RuMOFNSs by covalent amide bonds, acting as FL and ECL intensity switches. The target DNA, complementary to the probe DNA, triggers cyclic amplification of the target by nucleic acid exonuclease III (Exo III), repelling tetraferrocene reporter groups away from RuMOFNSs and inhibiting the electron transfer process and photoinduced electron transfer (PET) effect. These phenomena induce a double turn-on of FL and ECL signals with a high signal-to-noise ratio. The developed FL-ECL dual-mode sensing platform provides sensitive detection of the K-ras gene with detection limits of 0.01 fM (the detection range is 1 fM to 1 nM) and 0.003 fM (the detection range is 0.01 fM to 10 pM), respectively. In addition, the proposed dual-mode sensor can be easily extended to detect other disease-related biomarkers by changing the specific target and probe base sequences , depicting potential applications in bioanalysis and early disease diagnosis.
研究领域:
生物化学
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分析化学
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生物技术
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL RESEARCH
·
2024
·
IF 7.7
Xuan Zhu,Yi-Fan Zhao,Hong-Jian Wen,Yu Lu,Shuai You,Richard Ansah Herman,Jun Wang
Silkworm pupae, by-product of sericulture industry, is massively discarded. The degradation rate of silkworm pupae protein is critical to further employment, which reduces the impact of waste on the environment. Herein, magnetic Janus mesoporous silica nanoparticles immobilized proteinase K mutant T206M and Mucor circinelloides aspartic protease were employed in the co-degradation. The thermostability of T206M improved by enhancing structural rigidity ( t 1/2 by 30 min and T 50 by 5 °C), prompting the degradation efficiency. At 65 °C and pH 7, degradation rate reached the highest of 61.7%, which improved by 26% compared with single free protease degradation. Besides, the immobilized protease is easy to separate and reuse, which maintains 50% activity after 10 recycles. Therefore, immobilized protease co-degradation was first applied to the development and utilization of silkworm pupae resulting in the release of promising antioxidant properties and reduces the environmental impact by utilizing a natural and renewable resource.
研究领域:
生物技术
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环境科学
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蛋白质工程
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产品关联
DOI
PubMed
高影响因子
BIOELECTROCHEMISTRY
·
2024
·
IF 4.8
Haotian Xie,Zhaojiang Yin,Guobin Wei,Binghui Li,Hanfeng Cui,Hao Fan,Jing Zhang
This study developed a dual-mode “on-off-on” sensor based on a bipyridine ruthenium metal–organic framework (Ru-MOF) and dual enzyme cleavage technology for the sensitive detection of the K-ras gene. The sensor combines electrogenerated chemiluminescence (ECL) and fluorescence (FL) detection modes, achieving high sensitivity and specificity in detecting the K-ras gene through catalytic hairpin assembly (CHA) and dual enzyme cleavage reactions. Experimental results showed that the detection limits for the K-ras gene were 0.044 fM (ECL) and 0.16 fM (FL), demonstrating excellent selectivity and stability during detection. Through testing actual samples, the sensor has shown potential for application in complex biological environments. This method offers an efficient and reliable new tool for cancer diagnosis and treatment.
研究领域:
生物传感
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分子诊断
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癌症研究
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产品关联
DOI
PubMed
高影响因子
CHEMICAL PAPERS
·
2024
·Vol. 78
·期 4
·2275-2285
·
IF 2.1
Shu Ronglu,Du Yifan,Cao Liping,Zhao Hui,Luo Xiaoying,Wang Wei
Cerium samarium oxides catalysts with K + doping on the wall surface of cordierite honeycomb ceramics were preparation by sol–gel and dipping processes. Scanning electron microscopy, energy dispersive X-ray spectroscopy, X-ray diffraction and Fourier transform-infrared spectroscopy were used to characterize the structural morphology and phase compositions. Through XPS analysis and H 2 -TPR measurement of monolithic catalyst, it is found that potassium ions improve the oxygen mobility in CeO 2 lattice and form surface active oxygen, increase the amount of oxygen adsorbed on the surface and improve the oxidation–reduction capacity. The catalytic combustion research demonstrates that T 10 , T 50 and T m in samples (Ce/Sm-0 K, Ce/Sm-0.3 K, Ce/Sm-0.5 K and Ce/Sm-0.7 K) decrease significantly compared with the blank reference (Cordierite). And the optimized sample Ce/Sm-0.5 K possess the lowest T 10 (389.6 °C), the lowest T 50 (425.5 °C), and the lowest T m (419 °C) in comparison with other samples. The cycle stability investigation reveals that sample Ce/Sm-0.5 K has excellent structural stability and stable catalytic performance, which has potential application in soot catalytic combustion field.
研究领域:
催化化学
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材料科学
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环境工程
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化学工程
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表面科学
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DOI
高影响因子
Soft Matter
·
2023
·Vol. 19
·期 26
·4880-4890
·
IF 3.4
Yan-Lin Wang,Xi Wang,Hai-Rong Yu,Ting Liang,Xing-Bin Lv,Chang-Jing Cheng
Potassium ions (K+) play crucial roles in many biological processes. Abnormal K+ levels in the body are usually associated with physiological disorders or diseases, and thus, developing K+-sensitive sensors/devices is of great importance for disease diagnosis and health monitoring. Herein, we report a K+-sensitive photonic crystal hydrogel (PCH) sensor with bright structural colors for efficient monitoring of serum potassium. This PCH sensor consists of a poly(acrylamide-co-N-isopropylacrylamide-co-benzo-15-crown-5-acrylamide) (PANBC) smart hydrogel with embedded Fe3O4 colloidal photonic crystals (CPCs), which could strongly diffract visible light and endow the hydrogel with brilliant structural colors. The rich 15-crown-5 (15C5) units appended on the polymer backbone could selectively bind K+ ions to form stable 2 : 1 [15C5]2/K+ supramolecular complexes. These bis-bidentate complexes served as physical crosslinkers to crosslink the hydrogel and contracted its volume, and thus reduced the lattice spacing of Fe3O4 CPCs and blue-shifted the light diffraction, and finally reported on the K+ concentrations by a color change of the PCH. Our fabricated PCH sensor possessed high K+ selectivity and pH- and thermo-sensitive response performances to K+. Most interestingly, the K+-responding PANBC PCH sensor could be conveniently regenerated via simple alternate flushing with hot/cold water due to the excellent thermosensitivity of the introduced PNIPAM moieties into the hydrogel. Such a PCH sensor provides a simple, low-cost and efficient strategy for visualized monitoring of hyperkalemia/hypokalemia, which will significantly promote the development of biosensors.
研究领域:
生物传感器
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材料科学
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分析化学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
GREEN CHEMISTRY
·
2014
·Vol. 16
·期 5
·2762-2770
·
IF 6.852
Zehui Zhang,Bing Liu,Kangle Lv,Jie Sun,Kejan Deng
In this study, we have successfully prepared a heterogeneous catalyst (K-10 clay-Mo) by the immobilization of bis(acetylacetonato) dioxo-molybdenum(VI) [MoO2(acac)2] on montmorillonite K-10 clay. The structure of the resultant catalyst was characterized by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), transmission electronic microscopy (TEM), and Fourier transform infrared (FTIR) spectroscopy. The catalytic activity of K-10 clay-Mo was tested in the aerobic oxidation of 5-hydroxymethylfurfural (HMF). Although a molecule of HMF contains one hydroxyl group and one aldehyde group, to our surprise, the catalyst showed high catalytic activity for the oxidation of the aldehyde group of HMF into 5-hydroxymethyl-2-furancarboxylic acid (HMFCA). A variety of important reaction parameters such as the reaction temperature, catalyst amount, and solvent were explored. HMFCA could be obtained in a high yield of 86.9% with a HMF conversion of 100% after a short reaction time of 3 h in toluene. More importantly, the catalyst K-10 clay-Mo could be reused several times without a significant loss of its catalytic activity.
研究领域:
化学工程
·
催化材料
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DOI
高影响因子
Small
·
2025
·
IF 12.1
Mingyang Zhong,Tevin Li,Shouyi Yuan,Junwei Lucas Bao,Yonggang Wang
Small Quinone-based Organic Electrodes have received extensive attention. However, the rapid dissolution of small quinone electrode into electrolyte impede their practical applications. For example, Anthraquinone (AQ) and Phenanthrenequinone (PQ) molecules have been rarely reported for aqueous batteries due to the inferior electrochemical performance. Herein, a high-concentration hybrid electrolyte containing 1m Zn(OTF) 2 and 15m KOTF salts is introduced for small quinone electrode and investigate the redox behavior of PQ and AQ electrode is investigated in Zn 2+ /K + hybrid electrolyte. These findings reveal that AQ with carbonyl groups at para-position selectively stores proton rather than K + , while PQ with carbonyl group at ortho-position simultaneously stores proton and K + without selectivity. Moreover, the Zn(OTF) 2 salt serves as both proton buffer and binder to improve the cycle stability. Consequently, when PQ is paired with Zn metal anode for Zn || PQ batteries, the addition of potassium salt can improve the energy density of the Zn batteries. Moreover, when PQ is paired with Prussian blue cathode to assemble aqueous K-ion batteries, the addition of zinc salt in the electrolyte can improve the cycle stability via binding discharged PQ molecules with divalent-ions. This research provides an electrolyte engineering strategy to address the challenges facing PQ electrode for aqueous batteries.
研究领域:
储能
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电化学
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材料科学
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有机电极
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电池技术
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产品关联
DOI
PubMed
高影响因子
International Journal of Nanomedicine
·
2018
·
IF 4.37
Xin Jin,Qing Yang,Ning Cai
Introduction Ginsenoside compound K (CK) has effects on cell-cycle regulation, tumor growth inhibition, and apoptosis induction. However, it has limited applications in clinical settings because of its low solubility and poor absorption. Methods To overcome these limitations, we aimed to develop a mixed micellar system composed of phosphatidylcholine (PC) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine polyethylene glycol 2000 (DSPE PEG 2000; DP). CK encapsulated in PC/DP mixed micelles had enhanced solubility, permeability, and retention effects. Results Compared to free CK, the CK PC/DP micellar system exhibited improved anticancer effects in vitro, including cell-cycle arrest, apoptosis, and anti-invasion in human lung carcinoma A549 cells. The significant proapoptotic effect was reflected by increased chromosomal condensation, annexin V/propidium iodide staining, and related protein expression. In vitro cellular uptake and optical mouse imaging in vivo suggested that the improved antitumor effect was caused primarily by enhanced uptake and tumor targeting. Furthermore, an in vivo antitumor efficacy study indicated that the CK mixed micelles significantly inhibited tumor growth, thereby decreasing tumor volume at the end of the experiment as compared with that in the control mice. Histological analysis confirmed the antitumor effect with low toxicity. Conclusion The PC/DP micellar system was an effective drug delivery system for CK in tumor therapy.
研究领域:
药学
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肿瘤学
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药物递送系统
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药理学
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产品关联
DOI
PubMed
高影响因子
Solar RRL
·
2021
·Vol. 5
·期 9
·2100292
·
IF 8.582
Junzhuo Li,Zhaohui Ruan,Lei Zhu,Yudong Li,Xianzhu Xu,Yulin Yang,Yuan Yuan,Kaifeng Lin
Heteroatoms doping and morphology design have been considered as effective approaches to enhancing the photocatalytic activity of graphitic carbon nitride (g-C 3 N 4 ), whereas few of them have focused on the synergistic effect of codoping and the optical property of the porous structure during the catalytic process. Herein, a porous g-C 3 N 4 sphere codoped with K and I is designed and prepared to investigate the different roles played during the reaction. Compared with the pristine carbon nitride, the activity of the prepared catalyst increased by 7.9 times to 75.2 μmol h −1 . Experimental results with density function theory calculations indicate the different roles of two elements played during the photocatalysis process and optical simulations are given to prove the enhancement of the porous structure in light absorption ability. The pore structure improves the light absorption and mass transfer capabilities of the catalyst, while the codoping of metal and nonmetal develop the separation capabilities of the carriers and optimize the energy band structure respectively, which synthetically lead to an enhanced performance of the photocatalysis.
研究领域:
材料科学
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催化
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纳米技术
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理论化学
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DOI
高影响因子
Nanoscale
·
2021
·Vol. 13
·期 9
·4911-4920
·
IF 7.79
Wenrui Wei,Yulong Zheng,Minghua Huang,Jing Shi,Lei Li,Zhicheng Shi,Shuai Liu,Huanlei Wang
Designing carbon anodes with rich heteroatoms and dilated graphitic interlayer spacing via a one-step synthesis process plays a vital role in accelerating the practical application of potassium ion batteries, but it is still a big challenge. Herein, P-doped S-rich mesoporous carbon (PSMC) is prepared by direct phosphate-assisted carbonization of carrageenan, and it exhibits excellent potassium storage capacity (449 mA h g−1 at 0.1 A g−1), superior rate performance (233 mA h g−1 at 2 A g−1) and long-term stability (97.3% capacity retention after 1000 cycles), due to the high sulfur doping (16.48 wt%) and the coexistence of ordered and disordered regions in the structure. Ex situ characterization, GITT and theoretical calculations reveal that the promotion of covalent sulfur can effectively increase the adsorption of K+ and enhance the K+ reaction kinetics. The proposed one-step synthesis strategy demonstrates the precise use of the composition in biomass, enabling large-scale production of high-performance anodes for K+ storage.
研究领域:
材料科学
·
能量存储
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）