Analytical Methods
·
2017
·Vol. 9
·期 47
·6689-6697
·
IF 1.9
Danyu He,Shehong Li,Pu Zhang,Hongxia Luo
A graphene platform electrode (GPE) was prepared directly by transferring chemical vapor deposited (CVD) graphene films from Cu foils to polyethylene terephthalate substrates with the aid of polymethyl-methacrylate, a distinct advantage over previously developed methods. L-Cysteine (L-Cys) was polymerized on the GPE by using a one-step electrodeposition technique to form a poly-L-Cys/GPE. As a negatively charged polymer, the poly-L-Cys could adsorb positively charged dopamine (DA) and repel negatively charged ascorbic acid (AA) under optimum conditions. As a result, a novel electrochemical sensor for the simultaneous determination of DA and AA was developed. The electrochemical behaviors of DA and AA were investigated by cyclic voltammetry. Differential pulse voltammetry was used for determining DA and AA in their mixture, where the separation between oxidation peak potentials of DA and AA was about 163 mV. The broad linear responses to DA and AA were determined by chronoamperometry, which were 0.2 to 423 μM and 1 to 580 μM with detection limits (S/N = 3) of 0.0531 μM and 0.2031 μM, respectively. In addition, the as-obtained sensor delivered good stability as well as high resistance to interference, and was successfully utilized in determining real samples. Consequently, the poly-L-Cys/GPE could be considered as a new promising DA and AA sensor, strongly indicating that CVD graphene could be used successfully as a material in electrochemistry.
研究领域:
材料科学
·
分析化学
·
生物医学工程
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DOI
高影响因子
CHINESE JOURNAL OF POLYMER SCIENCE
·
2024
·Vol. 42
·期 5
·570-578
·
IF 4.1
Sun Xiao-Fei,Zhang Xu,Song Shu-Ping,Yao Ya-Qun,Zhang Yan,Wang Cheng-Liang,Sun Jing-Jiang,Wang Qing-Fu
L -glutamic acid (LA) is a bio-based, non-toxic, environmentally friendly material derived from biomass. The present study reports the application of Passerini three-component polymerization (P-3CP) for the straightforward preparation of LA-based light-responsive polyesters (PLTDs) under mild conditions. PLTDs with molar masses up to 8500 g/mol and high yields exceeding 90% are obtained. The chemical structures and light-responsive self-immolative behavior of PLTDs are comprehensively characterized by employing ultraviolet-visible (UV-Vis) spectroscopy, size exclusion chromatography (SEC), nuclear magnetic resonance (NMR) spectroscopy, and liquid chromatography mass spectrometry (LC-MS). Meanwhile, monodisperse PLTD-based doxorubicin-loaded nanoparticles (PLTD-DOX-NP) (size=193 nm, PDI=0.018) are formulated by nanoprecipitation method. Upon light-induced depolymerization, the PLTD-DOX-NP undergoes rapid decomposition, resulting in a burst release of 80% cargo within 13 s. Furthermore, according to biological toxicity tests, the PLTD-NP possesses adequate biosafety, both before and after irradiation. Overall, the incorporation of P-3CP with biorenewable LA-based monomer adheres to the principles of green chemistry, significantly simplifying the synthetic pathway of light-responsive polymers.
研究领域:
材料科学
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生物医学工程
·
绿色化学
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2013
·
IF 5.437
Xiaoyu Mu,Li Qi,Ping Dong,Juan Qiao,Jian Hou,Zongxiu Nie,Huimin Ma
Gold nanoclusters (Au NCs) possess outstanding physical and chemical attributes that make them excellent scaffolds for the construction of novel chemical and biological sensors. In this study, a simple one-pot synthesis method, employing l -proline as the stabilizer, was presented for preparation of fluorescent Au NCs. This strategy allowed the generation of water-soluble Au NCs within a short time of 15 min. The as-prepared Au NCs exhibited a bluish fluorescence emission at 440 nm and a quantum yield of 2.94%. Based on the aggregation-induced fluorescence quenching mechanism, the Au NCs provided favorable biocompatibility, high sensitivity and good selectivity for the measurement of ferric ion (Fe 3+ ). Furthermore, serum samples were analyzed for the serum iron contents by using this proposed biocompatible fluorescent sensor, indicating the potential value of this Au NCs-based fluorescent sensor for application in biological and clinical analysis.
研究领域:
分析化学
·
生物医学
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DOI
高影响因子
FIBERS AND POLYMERS
·
2018
·Vol. 19
·期 11
·2324-2334
·
IF 1.353
Xu Qingbo,Duan Panpan,Zhang Yanyan,Fu Feiya,Liu Xiangdong
In this work, we developed a new method that can achieve immobilization and protection of the Cu NPs coating on the cotton fabrics by a simple two-step impregnation method. Firstly, L-cysteine (Cys) was grafted onto cotton fabric via esterification with the hydroxyl groups of cellulose, then Cu NPs were introduced on the fabric surface in the presence of a protective reagent, citric acid. Due to the doubled stabilization acts of Cys and citric acid, the Cu NPs immobilized on the fabric surface showed an excellent antibacterial effect and outstanding laundering durability. As a result, the mean size of the Cu NPs coating on the cotton fabric is about 62.4 nm, and the modified cotton fabrics showed satisfactory antibacterial ability against both S. aureus and E. coli , which the bacterial reduction rates are all higher than 98 % even withstand 50 washing cycles. Therefore, this method to prepare antibacterial cotton fabrics showed great potential applications in socks, cosmetic, and medical textiles.
研究领域:
材料科学
·
纺织化学
·
纳米医学
·
表面功能化
·
抗菌材料
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DOI
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 34
·27003-27008
·
IF 3.84
Lin Zhang,Chunli Xu,Guoxin Song,Baoxin Li
A simple protocol to distinguish enantiomers is extremely intriguing and useful. Herein, we report on a method for the visual recognition of 3,4-dihydroxyphenylalanine (Dopa) enantiomers. It is based on the chirality of L-cysteine-capped gold nanoparticles (L-Cys-capped AuNPs) that can be used as a chiral selector for L- and D- forms of Dopa. On addition of L-Dopa to a solution of the L-Cys-capped AuNPs, a color change from red to blue can be seen, while no color change is found on addition of D-Dopa. The chiral recognition can be achieved by eye and simple spectrophotometry. Notably, this method does not require complicated chiral modification. The method excels through its low-cost, good availability of materials, and its simplicity.
研究领域:
纳米生物学
·
分析化学
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DOI
高影响因子
FLUID PHASE EQUILIBRIA
·
2013
·
IF 2.379
Yumei Kai,Yonghong Hu,Zheng Cao,Xiang Liu,Yan Liu,Wenge Yang
The solubilities of l -malic acid in pure and mixture organic solvents were measured from 278.15 to 323.15 K using the laser monitoring observation system. The modified Apelblat equation, the semi-empirical Buchowski–Ksiazczak λh equation and the Combined Nearly Ideal Binary Solvent/Redlich (CNIBS/R-K) equation were proposed to correlate the experimental data. In pure and mixed solvents, the 100RD (relative deviation) <3.61 and 7.58, respectively, the 10 4 RMSD (root-mean-square deviations) <3.07 and 2.28, respectively, so the equations selected in this work fit the data very well. Van’t Hoff equation was used to predict the dissolution enthalpy and entropy of l -malic acid in the corresponding solvents.
研究领域:
药物化学
·
物理化学
·
溶液热力学
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药物结晶
·
分子药剂学
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DOI
高影响因子
Inorganic Chemistry Frontiers
·
2025
·
IF 6.4
Le Hong,Lina Ding,Zhenyu Ma,Lin Wang,Kun Wang,Weinan Dong,Manlin Qi,Yujia Shi,Chenyu Liu,Xiaolin Sun,Xue Bai,Chunyan Li,Zhennan Wu
Fluorescent nanosensors based on ligand-engineered gold nanoclusters (Au NCs), guiding antibiotic therapy, offer a promising approach for rapid bacterial detection. Here, we developed dual-ligand-modified Au NCs with 6-azido-2-thiothymidine (ATT) and L-arginine (Arg), in which Arg modification enhanced the photoluminescence (PL) intensity compared to ATT-Au NCs. This enables a linear response (R2 > 0.97) to bacterial concentrations (105–109 CFU mL−1) within 30 minutes. Targeted metabolomics revealed that bacterial Arg deiminase metabolized Arg to citrulline, triggering PL quenching. Furthermore, it demonstrates the quantitative relationship between bacterial metabolic status and alterations in PL signals. The signal changes were visible under UV light and can also be quantified using a smartphone app (Light Meter). The detection limit reached 105 CFU mL−1. In vivo experiments confirmed that the probe can monitor wounds infected with Staphylococcus aureus (S. aureus), with minimal interference from biological matrices. This work highlights a portable, cost-effective strategy for point-of-care bacterial detection, addressing critical needs in clinical diagnostics.
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Qi Qi,Yaqian Deng,Sichen Gu,Min Gao,Jun-ya Hasegawa,Guangmin Zhou,Xiaohui Lv,Wei Lv,Quan-Hong Yang
A binder plays an important role in stabilizing the electrode structure and improving the cyclic stability of batteries. However, the traditional binders are no longer satisfactory in lithium–sulfur (Li–S) batteries because of their failure in accommodating the large volume changes of sulfur and trapping soluble intermediate polysulfides, thus causing severe capacity decay. In this work, we prepared a multifunctional binder for Li–S batteries by merely modifying the acacia gum (AG), a low-cost biomass polymer, with l-cysteine under mild conditions. Owing to the introduced amino and carboxyl branches by the l-cysteine, the modified AG shows enhanced polysulfide trapping ability and can effectively restrain the shuttling of polysulfides. In addition, the introduction of branches can help form a cross-linked 3D network with better mechanical strength and flexibility for adhering sulfur and accommodating the volume changes of cathode materials. As a result, compared with the normally used polyvinylidene fluoride binder and the unmodified AG binder, the l-cysteine-modified AG binder effectively enhanced the rate capability and cycling stability of the Li–S batteries directly using sulfur as the cathode, showing a promising way to prompt the practical use of Li–S batteries.
研究领域:
材料科学
·
电化学
·
能源存储
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DOI
高影响因子
CHEMISTRY & BIODIVERSITY
·
2023
·Vol. 20
·期 7
·e202300539
·
IF 2.9
Yi-Gui Qiu,Zi-Hui Yang,Xue-Bao Sun,Dao-Jun Jin,Yi-Ming Zheng,Jia Li,Wen Gu
To discover novel laccase inhibitors as potential fungicides, twenty-six novel L-menthol hydrazide derivatives were designed and synthesized. In the in vitro antifungal assay, most of the target compounds displayed pronounced antifungal activity against Sclerotinia sclerotiorum , Fusarium graminearum , and Botryosphaeria dothidea . Especially, the EC 50 of compounds 3 b and 3 q against B. dothidea was 0.465 and 0.622 mg/L, which was close to the positive compound fluxapyroxad (EC 50 =0.322 mg/L). Scanning electron microscopy (SEM) analysis showed that compound 3 b could significantly damage the mycelial morphology of B. dothidea. In vivo antifungal experiments on apple fruits showed that 3 b exhibited excellent protective and curative effects. Furthermore, in the in vitro laccase inhibition assay, 3 b showed outstanding inhibitory activity with the IC 50 value of 2.08 μM, which is much stronger than positive control cysteine and PMDD-5Y. These results indicated that this class of L-menthol derivatives could be promising leads for the discovery of laccase-targeting fungicides. Graphical
研究领域:
植物保护学
·
药理学
·
分子生物学
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DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2024
·
IF 7.1
Xuerui Jin,Chaoqun Huang,Can Cui,Huayi Liu,Zhenyu Liu,Dawen Niu,Yunzi Luo
Fluorinated amino acids play a crucial role as fundamental components in drug synthesis owing to their favorable attributes that enhance the pharmacokinetic properties of drugs. Nevertheless, the conventional chemical synthesis of fluorinated amino acids faces challenges in achieving stereoselectivity control. Additionally, the high cost of substrates and the intricate cofactor recycling process in enzymatic synthesis have limited their industrial production and applications. In this study, we developed a three-step chemoenzymatic cascade for the asymmetric synthesis of chiral fluorinated amino acids. The methodology involves the utilization of a variety of cost-effective aldehydes as starting materials, enabling economical aldol reactions catalyzed by aldolase. Subsequent chemical decarboxylation produces substrates for reductive amination through phenylalanine ammonia lyase, resulting in a diverse library of fluorinated aromatic l-α-amino acid products with a relative high yield and high enantiomeric purity (up to 99%). Furthermore, we synthesized three pharmaceutically relevant fluorinated l-α-amino acids on a preparative scale using whole-cell biocatalysts, demonstrating the versatility of the approach. This study has introduced a simplified, efficient, and cost-effective route for synthesizing fluorinated l-α-amino acids under mild conditions, offering insights into the production of valuable fluorine-containing pharmaceuticals and other biologically active compounds.
研究领域:
有机化学
·
生物化学
·
药学
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DOI
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 51
·45829-45834
·
IF 3.289
Xia Lin,Qinghong Wang,Shu Zhu,Juanjuan Xu,Qiao Xia,Yingzi Fu
In this article, a sensitive and stereo-selective biosensor for L-glutamic acid (L-Glu) based on the electrochemiluminescence (ECL) of Ru(bpy)32+ has been designed by applying L-glutamic dehydrogenase (GLDH) for enzymatic generation of NADH in situ. The novel ECL Glu biosensor was prepared by immobilizing GLDH on the modified electrodes with Ru(bpy)32+–platinum nanoparticles (Ru–PtNPs) and reduced graphene oxide–multiwall carbon nanotubes–Nafion (rGO–MWCNT–Nf). Fourier transform infrared spectroscopy (FT-IR), electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM) and cyclic voltammetry (CV) were employed to investigate the characteristics of different materials. In addition, the ECL technology was adopted to explore the interactions between the prepared Glu biosensor and L- or D-Glu. A remarkable difference of ECL intensity was observed when the proposed biosensor interacted with L- and D-Glu, respectively, and good responses were obtained from L-Glu in a range from 1.0 × 10−7 M to 5.0 × 10−3 M with a low detection limit of 3.3 × 10−8 M (S/N = 3). The developed ECL biosensor showed satisfying sensitivity, selectivity, reproducibility and stability, therefore, it might provide a new perspective for the recognition and detection of L-Glu.
研究领域:
生物化学
·
分析化学
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DOI
高影响因子
CHEMOSPHERE
·
2022
·
IF 8.943
Zhi Li,Qingxiang Zhou,Shuangying Li,Menghua Liu,Yanhui Li,Chunmao Chen
In this study, a label-free “turn off” fluorescent sensor has been resoundingly fabricated using carbon dots (CDs) for ultrasensitive detection of copper ions (Cu 2+ ). CDs are prepared by solid phase carbonization method using p-toluidine and l -cysteine as the precursors. The synthesized CDs exhibited the highest fluorescence intensity with excitation and emission wavelengths set at 300 nm and 400 nm, respectively. The CDs were selective and sensitive to Cu 2+ due to the static quenching mechanism. The concentration of CDs, and solution pH and incubation time were important parameters for the developed sensor. The experimental results showed that 20 mgL −1 was enough for the analysis. As the solution pH was concerned, it was apparent that the sensor was endowed with an excellent response signal to Cu 2+ and provided high sensitivity at pH 12. The interaction occurred very quickly, and the incubation time could be set at 1 min. The sensor provided a two-stage calibration curve to Cu 2+ in the range of 0.05–0.7 and 0.7–4 μM with a limit of detection of 47 nM. The obtained results clearly demonstrated that this facile method was fast, reliable and selective for detecting Cu 2+ , which would explore a prospective strategy for developing effective and low-cost sensors for monitoring metal ions in aqueous environments.
研究领域:
分析化学
·
环境科学
·
材料科学
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DOI
高影响因子
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