BIOCONJUGATE CHEMISTRY
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2025
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IF 3.9
Simin Chen,Guizhen Xu,Xiao Li,Yixian Peng,Yuanfang Feng,Jianqiong Pan,Qingao Meng,Shanni Hong,Xiahui Lin
The tumor microenvironment (TME) exhibits metabolic dysfunction characterized by lactate (LA) accumulation, which leads to tumor progression, angiogenesis, and therapy resistance. Targeting LA metabolism through lactate oxidase (LOX) converting LA to pyruvate and hydrogen peroxide (H2O2) under aerobic conditions is a promising therapeutic strategy. However, LOX activity is limited by TME hypoxia. To overcome this limitation, we developed an integrated nanotheranostic system based on hollow MnO2 nanoparticles loaded with LOX and cinnamaldehyde (CA) and modified with hyaluronic acid (HA) for tumor-targeted delivery (denoted as MCLH). In the acidic TME, MCLH decomposes to release LOX and CA, while MnO2 reacts with endogenous H2O2 to generate O2. The resulting O2 maintains LOX-mediated LA oxidation, thereby providing additional H2O2 to promote further O2 production, establishing a self-sustaining cycle that continuously consumes lactate. Meanwhile, CA depletes glutathione via Michael addition, disrupting redox homeostasis and enhancing H2O2 accumulation to increase oxidative stress. The released manganese ions (Mn2+) also enable magnetic resonance imaging (MRI) contrast for real-time monitoring. These cascading effects collectively achieve the synergistic regulation of LA metabolism and oxidative damage, providing an effective strategy for tumor treatment.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Hao Huang,Ping Ying,Ye Wang,Qinghong Wu,Lei Wang,Xizhe Fu
Melastoma dodecandrum Lour. (MDL) contains abundant anthocyanins. Choline chloride-oxalic acid (ChCl-OA, 1:1) was selected for the temperature-dependent convection induced incremental extraction of anthocyanins from MDL. The regulating effects of MDL anthocyanins on gut microbiota were studied by analyzing the diversity in C57BL/6 J mice. The results showed that under optimized conditions (ultrasonic power of 310.4 W, extraction temperature of 59.9 °C, cold-stimulation time of 9.0 min, extraction time of 29.8 min), the yield of anthocyanins (Y A ) achieved the highest level at 60.28 ± 1.05 mg CGE/g DW, outperforming non-incremental approach by 58 %. The ratio of Y A obtained from the fifth regeneration of ChCl-OA to initial Y A was over 90 %. Eight anthocyanins were identified in incremental extracts, over four anthocyanins in non-incremental extracts. The ChCl-OA system achieved equilibrium at 3.5 ps with energy of −1002.22 kcal/mol. More hydrogen bonds number (128) and longer lifetime (6.72 ps) resulted in a larger diffusion coefficient of anthocyanins at 0.10 m 2 /s. The 60 mg/mL of anthocyanins increased the α-Diversity (Sobs) and Gut Microbiome Health Index (GMHI) by 22 % and 56 %, respectively, harmonizing the gut microbiota. Therefore, this recyclable ChCl-OA based incremental extraction has unveiled horizons for the green and sustainable production of MDL anthocyanins.
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STAR Protocols
·
2025
·
IF 1.3
Yu Ran,Qiqing Yang,Lianqi Zhou,Heyu Li,Bing Yang,Long Zhang
Summary L-lactate has been recognized as an essential molecule for signaling and metabolic balance. Lactylation, a post-translational modification (PTM) derived from L-lactate, is commonly observed on various proteins and plays essential roles in cellular processes. Here, we present a protocol to globally profile the lactylation proteome and perform label-free quantification. We provide steps for cell preparation, protein extraction, digestion, peptide desalting, and lactylpeptide enrichment. Additionally, we outline the parameters for liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. For complete details on the use and execution of this protocol, please refer to Li et al. 1
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ACS Sustainable Chemistry & Engineering
·
2025
·
IF 7.3
Ling Ding,Di Pan,Ke Du,He Li,Jiayu Yu,Wanyi Li,Wei Chen,Yahong Zhang,Yi Tang
Chemical recycling of poly(lactic acid) (PLA) to lactide (LD) monomers offers a dual benefit in terms of production and waste management. However, the efficiency of this recycling process is often hindered by harsh reaction conditions, high catalyst loadings, and product separation issues. In this study, ZSM-5 zeolite subcrystal (SC) with size smaller than 10 nm and ultrahigh external surface area was employed as a support to disperse zinc oxide (ZnOx) species. Different from nanosized ZSM-5 (nano-Z5) zeolite, various characterization results demonstrated that ZnOx nanoparticles on SC exhibited exceptionally high dispersion and small size, even at ultrahigh loadings. As a result, rapid depolymerization of PLA (∼97 molPLA molZn–1 h–1) and high recovery of LD (65%) can be achieved at a low Zn(II)/PLA mass ratio of 0.008 under optimum condition. However, it was found that it is preferable to minimize the use of the carrier (SC) on the basis of achieving a high dispersion of ZnOx. This can be assigned to the fact that excessive use of the carrier can hinder the accessibility of PLA to active sites due to diffusion limitations. This work contributes to advancing heterogeneous catalysis for the closed loop recycling of biodegradable plastic, thus promoting a sustainable circular economy.
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MOLECULAR AND CELLULAR ENDOCRINOLOGY
·
2025
·
IF 3.6
Dan-Dan Jin,Yu-Ying He,Feng Ran,Hong-Yuan Yang,Gui-Jun Yan,Zeng-Ming Yang
Decidualization of endometrial stromal cells is important for pregnancy in rodents and humans. Dysfunction of decidualization can lead to implantation failure and recurrent miscarriage. Although the amount of PGF2α in uterine fluid increases during embryo implantation, what stimulates the production of PGF2α in the uterus and whether PGF2α is involved in embryo implantation and decidualization are still unknown. In our study, human chorionic gonadotropin (hCG), an embryo-derived protein, stimulates human endometrial epithelial cells to increase the levels of PGF2α synthesis-related enzymes (cPLA2, COX-1, COX-2, and AKR1C3) and to secrete PGF2α. Epithelial PGF2α induces EREG and HB-EGF secretion by activating ADAM17. The level of IGFBP1, a marker of human in vitro decidualization, is significantly increased by EREG or HB-EGF. PGF2α is also able to increase the IGFBP1 level in humans during in vitro decidualization. Our results indicate that hCG stimulates uterine epithelial PGF2α production to induce in vitro decidualization through EREG and HB-EGF.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Jiatao Zhang,Huanlong Liu,Yifeng Wu,Jia Guo,Yulu Li,Xiaojing Wang,Shaobo Li,Xueyan Yun,Tungalag Dong
The limited response sensitivity of chemically reduced graphene oxide (RGO) to ammonia (NH 3 ), along with its low limit of detection (LOD) and poor selectivity, significantly restricts its application in the detection of meat freshness. Here, we fabricated homogeneous monolayer RGO self-stacking membrane that exhibited both strong sensitivity and an ultra-low LOD as an NH 3 sensor. The outstanding performance is mainly attributed to the synergistic effects of a high surface area—offering numerous adsorption sites—and residual oxygen-containing functional groups, which promote strong interactions with NH 3 through hydrogen bonding, protonation, and chemical reactions involving -N-H bond dissociation. The comprehensive ex-situ spectroscopic characterizations also clearly demonstrated the adsorption-desorption kinetics of NH 3 . Furthermore, the RGO sensor demonstrated strong potential for monitoring the freshness of lamb, with its responses showing high correlation with total viable count (TVC) and total volatile basic nitrogen (TVB-N) levels. These results underscore the promise of non-hybridized, undoped RGO-based sensors for practical applications in meat freshness assessment.
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SOLID STATE SCIENCES
·
2025
·
IF 3.3
Kashif Ali Kalhoro,Chi Zhang,Atiq Ur Rehman,Di Wu,Muhammad Anwar,Adil Khan,Dokyoon Kim,Mohammadreza Shokouhimehr,Zhengchun Liu
Reliable, high-quality glucose monitoring remains a significant challenge in the food industry, underscoring the need to develop cost-effective, high-performance glucose sensors. Herein, the CuCo(CN) 6 nanocomposite was successfully synthesized at room temperature via a facile co-precipitation method and utilized as a non-enzymatic electrode for electrochemical glucose detection. Benefiting from its well-defined cyanide-bridged framework, CuCo(CN) 6 exhibited exceptional electrocatalytic performance for glucose oxidation, achieving a high sensitivity of 1513.60 μA mM −1 cm −2 , a broad linear range of 0.1–11 mM, and an ultralow detection limit of 0.45 μM. Moreover, it demonstrated a rapid response, excellent anti-interference capability, and stable operation over 16 days. Practical applicability was validated by determining glucose in commercial beverages (Black Tea, Pepsi, and Sprite; recoveries 94.44–103.70 %, RSD below 1.36 %) and human serum (recoveries 98.95–99.33 %, RSD not exceeding 1.04 %), demonstrating that CuCo(CN) 6 is a robust, high-performance platform for real-time glucose monitoring in both food and biological samples.
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RENEWABLE ENERGY
·
2025
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IF 9.1
Wei Liu,Wenruo Li,Jie Wang,Xinzhao Zhao,Tianyi Wang,Haoyu Zhan,Xiannian Sun,Naibao Huang
Proton exchange membrane fuel cells (PEMFC/PEMWE) are important devices for the conversion and utilization of renewable hydrogen energy. By electrodeposition in deep eutectic solvent (DES) mixed with critic acid (CA), a Nb-doped amorphous carbon coating (ACC) with enhanced conductivity was successfully grown on the surface of 316L stainless steel bipolar plates. The addition of Nb significantly lowered contact resistance of ACC to 13.8 mΩ cm 2 , while the Nb-doped ACC still exhibits excellent corrosion resistance and high electrochemical stability up to 300 h. Theoretical calculation and characterization indicate that the solvent shell structure formed between CA and Nb 5+ ions in the electrolyte facilitates the migration of Nb 5+ to the cathode, thereby promoting more Nb deposition. With the help of CA, the nucleation process of the coating shifts to instantaneous nucleation, favoring the formation of a dense and smooth coating. Moreover, the chemical adsorption of CA on the stainless steel surface enhances the adhesion between the ACC and the substrate. The excellent electrochemical property, strong interfacial adhesion, and high stability in service of the Nb-doped ACC indicates that the proposed electrodeposition method is a promising approach for fabricating ACC-modified stainless steel serving as bipolar plates.
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BIOSENSORS & BIOELECTRONICS
·
2025
·
IF 10.5
Jiacheng Li,Ming Zhang,Cailing Zhang,Yin Zhang,Wenbin Chen,Hao Qu,Jian Liu,Lu Wang
As obesity rates continue to rise, there is an increasing focus on reducing obesity through exercise. People are becoming more aware of the importance of weight loss through physical activity. However, the effectiveness of exercise can vary significantly among individuals, making it challenging to evaluate its impact. Therefore, establishing a reliable method for assessing exercise effectiveness is crucial for enhancing exercise quality and reducing obesity risk. It is noteworthy that the relationship between N-lactoyl-phenylalanine (N-Lac-Phe) and energy metabolism has garnered considerable attention. In this study, we developed a N-Lac-Phe biosensor by detecting L-lactic acid (L-Lac) and L-phenylalanine (L-Phe) based on Solution-Gated Graphene Field-Effect Transistors (SGGT). Our findings showed that the L-Lac and L-Phe biosensors exhibited excellent linearity within concentration ranges of 300 pM to 300 nM for L-Lac and 3 nM–1000 nM for L-Phe, with R 2 values of 0.9934 and 0.9897, respectively. The detection accuracies for these two types of SGGT biosensors were 91.63 ± 6.97% and 99.39 ± 8.53%, respectively. Using the established N-Lac-Phe, L-Lac, and L-Phe relationship model (NLL model), we could calculate the concentration of N-Lac-Phe in the RAW264.7 culture medium based on the concentrations of L-Lac and L-Phe. The biosensors demonstrated excellent accuracy and selectivity, indicating their potential for rapidly evaluating the effectiveness of exercise.
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Energy & Environmental Science
·
2024
·
IF 32.4
Yang Ding,Erming Feng,Siyuan Lu,Jianhui Chang,Caoyu Long,Sichao Tong,Hengyue Li,Junliang Yang
Residual stresses generated within perovskite films during the high-temperature annealing and cooling process are the key contributors to the reduction of device performance and lifespan deterioration. Herein, a strategy of surface micro-etching and reconstruction is developed to regulate the stresses in a triple-cation (formamidine, methylamine, and cesium) perovskite film. A precise stoichiometric mixture of L-lactic acid (LA) and isopropanol (IPA) is used to controllably dissolve the surface of the perovskite film, followed by octylammonium iodide (OAI) post-treatment, enabling a sinking reconstruction of 2D perovskite from the surface to bulk phase and achieving a benign transition from surface tensile stress to compressive stress, as well as a more matchable interface energy level. As a result, the target perovskite solar cells (PSCs) yield an obviously enhanced power conversion efficiency (PCE) of 25.54%, which is the highest reported PCE for triple-cation PSCs. Meanwhile, PSC modules with 10.4 cm2 achieve a PCE of 21.02%. Furthermore, the surface micro-etched and reconstructed PSCs exhibit superior stability, and the PSC devices without encapsulation can maintain 83% of original efficiency after 500 hour illumination at maximum power point tracking (MPPT) in a N2 atmosphere. This study provides a valuable avenue to improve PSC stability and efficiency by regulating residual stresses through surface micro-etching and reconstruction.
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GREEN CHEMISTRY
·
2024
·
IF 9.3
Xinyue Sun,Junjie Ni,Yuhan Lou,Peng Zhao,Yanyan Yu,Yilin Li,Qi Tang,Haipeng Yu,Yongzhuang Liu
Solvents with integrated functions enable directional lignin valorization for producing value-added chemicals or lignin polyols. In this study, a solvent-triggered directional lignin valorization process was developed based on the design of a biphasic deep eutectic solvent/dichloromethane (DES/DCM) system. The designed solvents exhibit a combined feature of chemical stabilization from ethylene glycol-based-DES and physical stabilization from a biphasic system. By establishing the DES/DCM, lignin was effectively functionalized into lignin polyols with high yield. Furthermore, by implementing a simple recycling procedure of the DCM phase, the reaction pathway was switched, leading to depolymerization and stabilization of the stabilized lignin polyols. This process resulted in an almost threefold increase in monomeric acetal yields. The effect of biphasic DES on functionalization or switched depolymerization of lignin was confirmed through model compound studies. Our findings demonstrate a directional lignin valorization route using the designed biphasic DES system, which offers potential applications in the production of value-added aromatic chemicals or potential intermediates for polymers.
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Nanomaterials
·
2024
·Vol. 14
·期 8
·719
·
IF 4.4
Xueyan Li,Zeyi Wu,Xiangyu Song,Denghua Li,Jiajia Liu,Jiatao Zhang
Nowadays, metal oxide semiconductor gas sensors have diverse applications ranging from human health to smart agriculture with the development of Internet of Things (IoT) technologies. However, high operating temperatures and an unsatisfactory detection capability (high sensitivity, fast response/recovery speed, etc.) hinder their integration into the IoT. Herein, a ternary heterostructure was prepared by decorating WO3nanoplates with Au and SnO2nanoparticles through a facial photochemical deposition method. This was employed as a sensing material for 3-hydroxy-2-butanone (3H-2B), a biomarker ofListeria monocytogenes. These Au/SnO2–WO3nanoplate-based sensors exhibited an excellent response (Ra/Rg= 662) to 25 ppm 3H-2B, which was 24 times higher than that of pure WO3nanoplates at 140 °C. Moreover, the 3H-2B sensor showed an ultrafast response and recovery speed to 25 ppm 3H-2B as well as high selectivity. These excellent sensing performances could be attributed to the rich Au/SnO2–WO3active interfaces and the excellent transport of carriers in nanoplates. Furthermore, a wireless portable gas sensor equipped with the Au/SnO2–WO3nanoplates was assembled, which was tested using 3H-2B with known concentrations to study the possibilities of real-time gas monitoring in food quality and safety.
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