JOURNAL OF POWER SOURCES
·
2023
·
IF 9.2
Tian Yan,Hao Lin,Yang Xu,Kaijie Ma,Le Liu,Jingyu Xi
In aqueous redox flow batteries (ARFBs), the redox reaction of K 4 [Fe(CN) 6 ]/K 3 [Fe(CN) 6 ] is commonly applied as an active material for the positive electrode . Its performance is restrained by inhomogeneous electrochemical activity and oxygen evolution side reactions. The activity of both the redox reaction and the side reaction lacks two-dimensional detection method. In this study, ion concentration variations on the electrode surface are converted into refractive index changes by a total internal reflection (TIR) imaging system , which enables characterization of the two-dimensional distributions of surface activity and oxygen evolution reactions (OER) onset potential on the ARFBs positive electrode. This method can obtain the two-dimensional maps of parameters of cyclic voltammetry (CV) and the onset potential of the OER. We validate the feasibility of this method by examining three different electrodes and an electrochemical stability test. This work offers a characterization tool for developing homogeneous electrodes with enhanced activity, high reversibility, and minimized OER side reactions.
研究领域:
电化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
CARBON
·
2022
·
IF 11.307
Xin Wang,Lei Zhao,Jinshuang Hu,Hua Wei,Xiaoyang Liu,Enshou Li,Shenghong Yang
Heteroatom doping except oxygen is the main method to improve the performance of carbon quantum dots (CQDs), the application is also limited by unpredictable biological negative effects and environmental risks from heteroatoms (N/P/S, etc.). To maximize the role of oxygen while avoiding the negative effects of other heteroatoms, herein, we rationally designed and synthesized a new type of carbon-oxygen quantum dots (COQDs) with high fluorescent performance aim to realize pH sensing and reactive oxygen species (ROS) scavenging. The toxicity test results from four levels of bacterias, Hela cells , Daphnia Magna, and zebrafish indicate that the designed COQDs possess high biocompatibility and low environmental risk. Based on the opposing response of COQDs and Rhodamine 6G (Rh 6G) fluorescent signal to pH changes in the range of 6.0–9.0, a COQDs-Rh 6G complex was constructed by combining Rh 6G for ratiometric pH sensing, and a scanning colorimetric analysis model was further developed for the rapid pH judgment of commercially available waters. The proposed COQDs and pH sensing system were also successfully applied for bioimaging and intracellular pH analysis, respectively. More importantly, the designed COQDs also exhibit excellent ROS scavenging ability, which can be used as an effective antioxidant with good biocompatibility .
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2025
·
IF 6.3
Jialin Cui,Shikun Su,Ruimao Li,Hui Xu,Wenhua Yang,Lizhen Zhao,Wei Qin,Bing Teng,Chen Hu,Shijia Sun,Wei Wang,Haiyang Zhang,Degao Zhong
This study successfully synthesized a melilite-structured multifunctional phosphor, CaLaAl 3 O 7 : Eu 2+ , Eu 3+ , through solid-state reaction under reducing atmosphere. The phosphor demonstrates dual-band emission with contrasting temperature-dependent luminescence behaviors: the blue emission (Eu 2+ , 443 nm) exhibits a sharp intensity decline with rising temperature, whereas the red emission (Eu 3+ , 616 nm) shows only a slight decrease in intensity under the same conditions. This unique property enables high-sensitivity fluorescence thermometry, achieving a maximum relative thermometric sensitivity (S r ) of 2.59 % K −1 at 303 K. Moreover, by utilizing the significant luminescence color changes induced by temperature variations, we have achieved wide-gamut visualization of surface temperature distributions on objects. Under 365 nm UV excitation, the phosphor demonstrates persistent blue luminescence lasting 180 s due to defect energy levels. By integrating multi-wavelength selective excitation, long afterglow, wide color gamut emission, and high sensitivity temperature response, CaLaAl 3 O 7 : Eu 2+ , Eu 3+ demonstrates promising applications in visual temperature detection and information encryption/anti-counterfeiting.
研究领域:
材料科学
·
发光材料
·
光学测温
·
光子学
·
防伪技术
✓ 已验证
产品关联
DOI
高影响因子
Chemistry-An Asian Journal
·
2025
·
IF 3.3
Yifan Liu,Feiran Liu,Tong Wu,Fei Yuan,Zhiyang An,Mingjing Sun,Jing Jing,Xiaoling Zhang
Monitoring viscosity dynamics in lipid droplets is crucial for understanding the pathogenesis of non-alcoholic fatty liver disease (NAFLD), a condition affecting 25% of the global population. However, the application of fluorescence lifetime imaging microscopy (FLIM) for this purpose is hampered by the lack of probes with long fluorescence lifetimes and high signal-to-noise ratios. Herein, we developed two naphthalimide-based dyed fluorescent probes, NBC and NBA, which are viscosity-sensitive and feature donor-π-acceptor architectures. They operate via the restriction of intramolecular rotation in high-viscosity environments exhibiting 10.7/9.6-fold fluorescence enhancement with strong linear correlations. In studies with HepG2 cells, both probes demonstrated excellent biocompatibility, specific targeting of lipid droplets, and remarkable photostability during prolonged imaging. FLIM analysis revealed dose-dependent increases in lipid droplet viscosity following oleic acid treatment. The fluorescence lifetimes increased from 1.3 to 7.0 ns for NBA and from 2.5 to 8.2 ns for NBC. Time-course experiments using the NBC probe captured distinct lipid droplet maturation stages, showing a progressive viscosity increase from newly formed droplets with a lifetime of 2.2 ns to mature droplets at 7.9 ns. These findings establish NBC and NBA as powerful tools for quantitative lipid droplet viscosity mapping, providing new insights into NAFLD pathogenesis and potentially enabling early diagnostic approaches for lipid metabolism disorders.
✓ 已验证
DOI
PubMed
高影响因子
IEEE SENSORS JOURNAL
·
2025
·
IF 4.5
Xiaolong Zhao,Huinan Zhang,Jie Liang,Qingchao Zhang,Yuetang Li,Tong Song,Zhenhua Li,Bo Li,Junbin Zang,Zhidong Zhang,Chenyang Xue
Pulse is a critical sign in evaluating physical health for the traditional Chinese medicine. A wearable flexible pressure sensor is a good scheme for human pulse signals monitoring. The flexible pressure sensors are required own high sensitivity and fast response time. Adding ionic gel to the dielectric layer of capacitive pressure sensors to form a double electric layer is an effective way to enhance the sensitivity and the resolution of the sensors. However, the fluidity and instability of liquid seriously hinder the sensor’s application. In this work, a ternary composite gel dielectric layer, composed by H2O, polyvinyl alcohol (PVA) and ionic liquids (ILs) with high flexibility and chemical stability, was prepared. Excellent self-healing and mechanical properties ensure the service life of the ionic gels. Assembled with patterned adhesive tape and two Ag electrodes, the sensor has a semi-hollow sensing chamber. A maximum sensitivity of 5.55 kPa-1 and a fast response time of 53 ms were observed. The sensor also exhibits remarkable mechanical stability over 3000 load-release cycles. This outstanding performance endows the sensor with promising potential for application in pulse detection. Besides, a multi-channel sensors array is proposed to reveal the pressure distribution in the spatial dimension.
研究领域:
生物医学工程
·
材料科学
·
传统中医
✓ 已验证
产品关联
DOI
高影响因子
IMMUNITY
·
2024
·
IF 25.5
Yangning Lan,Xiaoxuan Zhang,Shaorui Liu,Chen Guo,Yuxiao Jin,Hui Li,Linyixiao Wang,Jinghong Zhao,Yilin Hao,Zhicheng Li,Zhaoyuan Liu,Florent Ginhoux,Qi Xie,Heping Xu,Jie-Min Jia,Danyang He
Microglial reactivity to injury and disease is emerging as a heterogeneous, dynamic, and crucial determinant in neurological disorders. However, the plasticity and fate of disease-associated microglia (DAM) remain largely unknown. We established a lineage tracing system, leveraging the expression dynamics of secreted phosphoprotein 1（Spp1） to label and track DAM-like microglia during brain injury and recovery. Fate mapping of Spp1 + microglia during stroke in juvenile mice revealed an irreversible state of DAM-like microglia that were ultimately eliminated from the injured brain. By contrast, DAM-like microglia in the neonatal stroke models exhibited high plasticity, regaining a homeostatic signature and integrating into the microglial network after recovery. Furthermore, neonatal injury had a lasting impact on microglia, rendering them intrinsically sensitized to subsequent immune challenges. Therefore, our findings highlight the plasticity and innate immune memory of neonatal microglia, shedding light on the fate of DAM-like microglia in various neuropathological conditions.
研究领域:
神经科学
·
免疫学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Keting Zhou,Yu Xia
Mapping disulfide linkages is crucial for characterizing pharmaceutical proteins during drug development and quality control. Traditional bottom-up protein analysis workflows often suffer from incomplete mapping for tryptic peptides consisting of multiple disulfide bonds. Although the employment of a partial reduction of disulfide bonds can improve disulfide mapping, it becomes a bottleneck of analysis because individual tuning is often needed. Herein, we have developed an online disulfide-ene reaction system in which the composition of the reaction solvent can be programmed to achieve optimal partial reduction of tryptic disulfide peptides after liquid chromatography separation. By coupling this system onto a bottom-up protein analysis workflow, high coverage for sequencing (71–83%) and disulfide mapping (84–100%) was achieved for standard proteins consisting of 4–19 disulfide bonds. The analytical capability was further demonstrated by mapping 13 scrambled disulfide bonds in lysozyme and achieving compositional analysis of IgG isotypes (κ and λ) and subclasses (IgG1–IgG4) from human plasma.
研究领域:
生物化学
·
制药科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Cell Reports Methods
·
2023
·
IF 3.8
Jingtan Zhu,Xiaomei Liu,Jianyi Xu,Yating Deng,Pingfu Wang,Zhang Liu,Qihang Yang,Dongyu Li,Tingting Yu,Dan Zhu
Efficient labeling of the vasculature is important for understanding the organization of vascular networks. Here, we propose VALID, a vessel-labeling method that enables visualization of vascular networks with tissue clearing and light-sheet microscopy. VALID transforms traditional lipophilic dye solution into hydrogel by introducing gelatin and restrains the dye aggregation, resulting in complete and uniform vessel-labeling patterns with high signal-to-background ratios. VALID also enhances the compatibility of lipophilic dyes with solvent-based tissue-clearing protocols, which was hard to achieve previously. Using VALID, we combined lipophilic dyes with solvent-based tissue-clearing techniques to perform 3D reconstructions of vasculature within mouse brain and spinal cord. We also employed VALID for 3D visualization and quantification of microvascular damage in a middle cerebral artery occlusion mouse model. VALID should provide a simple, cost-effective vessel-labeling protocol that would significantly widen the applications of lipophilic dyes in research on cerebrovascular complications.
研究领域:
神经科学
·
生物医学工程
·
显微技术
·
血管生物学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Shuying Mao,Wei Jin,Sisi Fu,Keanqi Liu,Feng Xu,Linlin Wu,Yexin Xu,Huizhu Yang,Huafang Liu,Guangji Wang,Yan Liang
Numerous works have been focused on the bioactivities of protein hydrolysates (PHs) and their application in food or drug formulations, but their composition and pharmacokinetics have never been addressed due to their complex constitutes, short half-life, extremely low concentrations and lack of authentic standards. The present study aims to develop systematic analytical strategy and technical platform with optimized sample preparation, separation and detection protocols for PHs. Lineal peptides (LPs), extraction of the spleen of healthy pigs or calves, were used as cases. First, solvents with polarity gradients were used to globally extract peptides of LP from biological matrix. Non-targeted proteomics based on a high-resolution MS system was used to establish a reliable qualitative analysis workflow for PHs. Based on the developed approach, 247 unique peptides were identified using NanoLC-Orbitrap-MS/MS, and then further verified on the MicroLC-Q-TOF/MS system. In the quantitative analysis workflow, Skyline software was used to predict and optimize the LC-MS/MS detection parameters of LPs followed by investigating the linearity and precision of the developed analytical assay. Note worthily, we innovatively prepared calibration curves by sequential dilution of LP solution to overcome the bottleneck of lacking authentic standards and complex PH composition. All the peptides exhibited good linearity and precision in biological matrix. The established qualitative and quantitative assays were successfully applied to study the distribution characteristics of LPs in mice, and would be conductive to systematically map the profile and pharmacokinetics of peptides in various PHs in vivo and in vitro .
研究领域:
生物化学
·
药物分析
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Chemosensors
·
2023
·Vol. 11
·期 5
·270
·
IF 4.2
Song Wang,Xiaohui Yi,Ye Zhang,Zhiyi Gao,Ziyin Xiang,Yuwei Wang,Yuanzhao Wu,Yiwei Liu,Jie Shang,Run-Wei Li
Electronic skin (e-skin) has the potential to detect large-scale strain, which is typically achieved by integrating multiple strain sensors into an array. However, the latency and limited resolution of sensing have hindered its large-scale sensing applications. Here, we have developed a high-resolution detection sensing system capable of detecting static and dynamic strain with a simple fabrication process by combining capacitive and mechanoluminescent (ML) sensor units. An elastic polydimethylsiloxane (PDMS) composite film doped with ZnS:Cu and BaTiO3(BT) particles are fabricated as the functional film of the capacitive sensor. In contrast, the transparent electrode was fabricated on the surface of the as-prepared film. By incorporating BT nanoparticles into the elastic substrate, the ML intensity of the ZnS:Cu was improved up to 2.89 times that without BT addition, and the sensitivity of the capacitive sensor was increased as well. The capacitive part of the sensor presented a GF of 0.9 and good stability, while the ML part exhibited excellent performance, making it suitable for both static and dynamic sensing. Furthermore, the strain sensor integrated by 10 × 10 sensing units is demonstrated to detect large-scale strain with high resolution. Moreover, finger joint strain distribution tracking is achieved by attaching the strain sensor unit to the finger joint. With these characteristics, the e-skin may have great potential for bio-motion monitoring and human-computer interaction applications.
研究领域:
材料科学
·
生物医学工程
·
传感器技术
✓ 已验证
产品关联
DOI
高影响因子
Horticulturae
·
2025
·Vol. 11
·期 12
·1478
·
IF 3
Ling Su,Xinqi Li,Lixing Ning,Peng Shu,Qingyi Zhang,Zugen Liu,Xiong Peng,Huili Liu,Yuan Yuan,Dingbo Yuan,Guicheng Liu,Guangyong You,Junfang Chen,Xiaoman Liu,Yi Tao,Yanhong Feng,Jing Yang
Potato is globally recognized as the fourth most crucial staple food crop, trailing behind wheat, rice, and maize. Cadmium (Cd), a predominant heavy-metal pollutant in agricultural soils, demonstrates high biological toxicity and mobility. Therefore, exploring the genetic and molecular mechanisms underpinning cadmium tolerance in potato is of substantial theoretical and practical significance. In this research, an F2population composed of 170 families was established through the cross-breeding of homozygous diploid potato lines HD-5 (highly cadmium-tolerant) and M9 (cadmium-sensitive). Employing hydroponic cultivation, six traits, namely plant height (PH), root length (RL), shoot fresh weight (SFW), root fresh weight (RFW), chlorophyll content (SPAD), and nitrogen content (LNC), were measured in potato seedlings following a 9-day treatment with 40 mg·L−1CdCl2. By utilizing the high-density genetic map of this population for QTL mapping, a total of 35 genetic loci associated with cadmium tolerance in potato seedlings were identified. Notably, loci21 and loci22 on chromosome 9, loci29 on chromosome 10, and loci31 and loci33 on chromosome 12 were consistently detected across multiple environmental conditions. This reproducibility across environments suggests the phenotypic stability of these five loci, which are thus considered reliable and robust genetic determinants. In addition, transcriptome sequencing analysis of roots from parental lines HD-5 and M9 after cadmium treatment revealed that significantly differentially expressed genes between the two parents were associated with glutathione metabolism and photosynthesis. By integrating QTL mapping, transcriptome analysis, and gene annotation, we screened four candidate genes involved in cadmium tolerance regulation:DM8C09G01000(GST),DM8C09G01060(GST),DM8C09G02130(OXP1), andDM8C06G22960(PsaH). These findings provide molecular targets and a genetic basis for molecular breeding of cadmium-tolerant potato varieties.
研究领域:
植物学
·
遗传学
·
植物育种
·
分子生物学
·
环境胁迫生理学
✓ 已验证
产品关联
DOI
高影响因子
POLYMER
·
2026
·
IF 5.1
Fang Wang,Tingting Chen,Hanling Gu,Hang Heng,Xiao Hu
Elucidating crystallization in biodegradable polymer composite fibers requires both bulk thermal/structural analysis and spatially resolved characterization of local crystalline domains and phase distribution. Herein, one-dimensional poly(lactic acid)/silk fibroin (PLA/SF) composite nanofibers with different compositions were fabricated by electrospinning, and their isothermal crystallization behavior was systematically investigated using differential scanning calorimetry (DSC), X-ray diffraction (XRD), and Raman mapping spectroscopy. DSC analysis combined with Avrami kinetics demonstrated that silk fibroin acted as an effective bio-based nucleating agent, shortening the crystallization onset time and half-time while increasing the crystallization rate and crystallinity of PLA. Fibers crystallized at 105 °C exhibited faster crystallization and higher crystallinity than those treated at 125 °C, whereas treatment at 125 °C favored the formation of more ordered PLA α-crystals, as supported by temperature-dependent XRD results. Beyond the bulk information obtained from DSC and XRD, Raman mapping provided direct spatial visualization of PLA/SF phase distribution, local PLA crystalline regions, and silk fibroin β-sheet structures before and after isothermal treatment. The mapping results revealed good component dispersion without obvious phase separation and showed that isothermal crystallization generated finer and more dispersed crystalline domains within the composite fibers. This work demonstrates that integrating Raman mapping with thermal and diffraction analyses provides a powerful approach for revealing phase heterogeneity, localized crystallinity, and crystallization kinetics of electrospun biodegradable polymer-protein composite fibers.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）