FOOD CHEMISTRY
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2026
·
IF 10.4
Yike Han,Wenzhi Lei,Xingnan Wang,Hongcai Li,Yuanye Liu,Chunfeng Guo,Zhenpeng Gao
Aspergillus strain OA3 raised OTA/AFB 1 degradation to 95.56%/90.38%. • Inoculation elevated sweet/umami amino acids by 7.4%–42.1% vs. natural fermentation. • 93 volatiles with 40 odor activity values >0.1 dynamically rebuilt flavor profiles. • Inoculation enriched flavonoid biosynthesis (13vs.5)/degradation (7vs.3) vs. natural. • OA3-inoculated final product raised ABTS + · 25.1%, DPPH 5.7%, FRAP 6.8% vs. natural. This study compared Aspergillus strain OA3 inoculated fermentation and natural fermentation of Fu tea for safety, composition, flavor and metabolism. The OA3-inoculated group exhibited superior safety, with ochratoxin A and aflatoxin B 1 degradation rates reaching 95.56 ± 1.32% and 90.38 ± 3.48%, far higher than 68.93 ± 0.78% and 56.11 ± 3.48% in natural fermentation. Physicochemical components changed regularly during fermentation, with more flavor amino acids retained and stronger antioxidant activity in inoculated samples. Gradually accumulated ketones, esters and alcohols formed the characteristic flavor of Fu tea. Metabolomic analysis identified 158 metabolites throughout fermentation, with a more complex metabolic network at the late stage. Pathways of flavonoid degradation and flavone and flavonol biosynthesis were significantly enriched in OA3-fermented samples. Correlation analysis confirmed altered flavonoid levels closely correlated with improved antioxidant capacity. Aspergillus strain OA3 fermentation is an effective way to produce safer, high-quality Fu tea and facilitates targeted process optimisation. Download: Download high-res image (250KB) Download: Download full-size image
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高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Xu Wang,Xiaodan Li,Na Li,Ruidong Zi,Xin Hu,Yingying Qian,Yu Zhou
Matrix interference of dark tea was effectively eliminated by tea-specific clean-up. • Tea-specific pretreatment enabled rapid detections of AFB1 and OTA on tea sample. • A simple and cost-effective strategy was validated for rapid detection in dark tea. • Tea-specific pretreatment compatible with rapid test strips and instrumental analysis. Rapid test strips have been widely used for mycotoxins detection in foods; however, their application in tea is strictly limited by pronounced matrix interference. In this study, tea-specific cleaning methods were developed to enable rapid detection of aflatoxin B1 (AFB 1 ) and ochratoxin A (OTA) in dark tea and tea infusions. The proposed methods achieved recoveries ranging from 81.5% to 128.07% for AFB 1 and ranging from 80.05% to 131.48% for OTA. Method validation demonstrated robust accuracy and precision, with AFB 1 recoveries of 83.82%–110.00% (RSDs: 0.46%–12.74%) and OTA recoveries of 82.83%–118.47% (RSDs: 0.69%–11.74%). The limits of detection (LODs) were 1.14–2.52 μg/L for AFB 1 and were 1.13–3.04 μg/L for OTA in tea samples. The tea-specific clean-up effectively minimized matrix interference, yielding extracts compatible with rapid test strips for qualitative and quantitative testing. Overall, this study provides a simple, efficient and cost-effective strategy for improving the reliability of rapid mycotoxins detection in complex tea matrices.
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高影响因子
Analytical Methods
·
2025
·Vol. 17
·期 25
·5298-5307
·
IF 2.6
Yang Cao,Chun Huang,Kui Li,Xi Zhang,Mengfei Tong,Jiale Tu,Kun Yang,Shuai Yuan,Hong Zhang
To sensitively detect ochratoxin A (OTA), a CRISPR/Cas12a-assisted electrochemiluminescence (ECL) aptasensor based on COF@Ru coupled with a DNA tetrahedral scaffold (DTS) was successfully fabricated. The covalent organic framework (COF) acted as a confined micro-reactor for the tris(2,2′-bipyridyl)ruthenium(II)/tri-n-propylamine (Ru(bpy)32+/TPrA) system and hence, evidently enhanced ECL signals. Au nanoparticles (AuNPs) coupled with DTS caused more ferrocene (Fc)-modified quenching DNA probe molecules to be attached to the surface of the sensing electrode, which could reduce the detection background. Activated CRISPR/Cas12a could effectively amplify the ECL signal since it could cut off a considerable amount of quenching DNA probe molecules and make them move away from the electrode. Owing to these strategies, the fabricated ECL aptasensor could detect OTA as low as 3.5 fg mL−1 in a linear detection range of 10−5–100 ng mL−1. Furthermore, the CRISPR/Cas12a and COF@Ru coupled with DTS-based ECL biosensor possessed high stability and specificity. More importantly, the ECL biosensor could effectively detect OTA in food samples, indicating that it can play a role in monitoring OTA levels in the field of food safety.
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高影响因子
ANALYST
·
2022
·Vol. 147
·期 10
·2215-2222
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IF 5.227
Min Li,Hao Wang,Xiaodi Yu,Xindi Jia,Chao Zhu,Jiahui Liu,Fan Zhang,Zilei Chen,Mengmeng Yan,Qinzheng Yang
The enzyme-linked apta-sorbent assay (ELASA) is widely used for the detection of small-molecule compounds as a result of low cost and reagent stability of aptamers. However, enzyme labels used in ELASA still suffer from some drawbacks, such as high production cost and limited stability. To overcome the drawbacks, we reported a nanozyme-linked apta-sorbent assay (NLASA) coupled with surface-enhanced Raman scattering (SERS)-colorimetric dual-mode detection. For nanozyme labels, Pd–Pt bimetallic nanocrystals (Pd–Pt NRs) could catalyze 3,3′,5,5′-tetramethylbenzidine (TMB) to blue TMB2+, whose color variation could not only be distinguished by naked eyes but also had a strong SERS signal. The NLASA method was employed to detect ochratoxin A (OTA) with a limit of detection values of 0.097 nM (0.039 ppb) and 0.042 nM (0.017 ppb) via the colorimetric and SERS methods, respectively. This method was applied for the determination of OTA in wine and grape samples, and the detection results were in a satisfied agreement with those determined by the high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) method. The proposed NLASA method provided a rapid and sensitive detection for OTA and could also be broadened for other small-molecules.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Laicui Song,Jianlin Li,Hao Li,Yunwei Chang,Shijie Dai,Ruimin Xu,Menghua Dou,Qianjin Li,Guangping lv,Tiesong Zheng
A highly sensitive surface-enhanced Raman spectroscopy (SERS) aptasensor for multiplex mycotoxins detection was developed based on aptamer-functionalized photonic microsphere array. Gold nanoparticles (AuNPs) were grafted covalently onto the silica photonic crystal microsphere (SPCM) to fabricate the SERS active substrate, on which the near-field enhancement can reach to 20 times analyzed by the simulation electric field distribution. Two dyes of 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB) and nile blue A (NBA) were employed to make the SERS nanotags on AuNPs that were modified with corresponding anti-aptamer sequences. A SERS assay system was established based on the competitive reaction between the anti-aptamer on SERS nanotags and the target mycotoxin towards the aptamer on SPCM. The developed method showed a linear relationship in the range of 0.01–100 ng/mL for aflatoxin B 1 (AFB 1 ) and 0.001–10 ng/mL for ochratoxin A (OTA), and a low limit of detection of 0.36 pg/mL for AFB 1 and 0.034 pg/mL for OTA. The accuracy of such method was confirmed by its good recovery ratio and high repeatability, and further verified by the classic enzyme-linked immunosorbent assay using naturally mycotoxins contaminated edible samples. The established method has a great application potential for screening mycotoxins rapidly with high sensitivity, low-cost and high throughput in the fields of food and traditional Chinese herb.
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BIOSENSORS & BIOELECTRONICS
·
2022
·
IF 12.545
Hao Wang,Bianbian Zhao,Yufei Ye,Xiaoyu Qi,Yuting Zhang,Xiaole Xia,Xiaoli Wang,Nandi Zhou
A fluorescence and surface-enhanced Raman scattering (FL-SERS) dual-mode aptasensor was established for sensitive and rapid detection of Ochratoxin A (OTA). This aptasensor was assembled by the hybridization of OTA aptamer (OTA Apt) modified gold nanostars (Apt-AuNSs) and Cy3 modified complementary DNA functionalized gold nanospheres (cDNA-AuNPs). The aptasensor displays a low FL signal due to the proximity of Cy3 to AuNSs, and a high SERS signal due to the “hot spot” effect generated by the nano-gap between AuNSs and AuNPs. In the presence of OTA, the preferential combination of OTA Apt and OTA results in a low SERS signal. Meanwhile, cDNA-AuNPs are released from the hybrid complex, leading to the recovery of the FL signal. The aptasensor has a low detection limit of 0.17 ng/mL (the detection range is 1–100 ng/mL) in FL mode and 1.03 pg/mL (the detection range is 5–250 pg/mL) in SERS mode. Furthermore, this aptasensor exhibits high specificity and good interferent resistance, which can discriminate OTA from other mycotoxins . Moreover, this aptasensor can be used for OTA detection in coffee and wine samples with good recovery rates. Unlike single-signal aptasensors, this dual-mode aptasensor holds exquisite properties such as high reliability, great detection flexibility and good anti-interference ability.
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高影响因子
NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 46
·期 36
·17479-17486
·
IF 3.925
Zhenzhen Cui,Liang Guo,Zhenyu Jin,Lele Ma,Huaixia Yang,Mingsan Miao
Ochratoxin A (OTA) seriously affects the quality of herbal medicines and threatens the health of patients. Therefore, accurate detection of OTA in herbs is of great significance. Here, we fabricated a signal-off fluorescent biosensor for the highly sensitive detection of OTA by the activator regenerated by electron transfer atom transfer radical polymerization (ARGET ATRP) signal amplification technique. First, the specific aptamer sequence was immobilized on carboxylic MBs by an amide reaction. At the same time, the initiator was attached to the cDNA. Then the aptamer and cDNA formed a double helix structure through complementary base pairing. Afterwards, numerous fluorescent monomers were grafted onto the cDNA by ARGET ATRP, which emitted strong fluorescence. Upon addition of OTA, it could be specifically recognized by the aptamer and displace the cDNA, generating fluorescence quenching. Under optimal conditions, the detection limit of the as-prepared sensor was 7.6 fg mL−1 with a detection range of 2 × 10−3 ng mL−1 to 2 × 103 ng mL−1. Furthermore, this sensor exhibited high selectivity and reproducibility, and was successfully applied to the detection of OTA in samples of forsythia, astragalus, licorice, xanthium and malt. The outstanding performance of this biosensor suggests its possible application in the detection of mycotoxin in herbal medicine samples.
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高影响因子
ACS Omega
·
2021
·
IF 3.512
Linzhi Li,Xing Liu,Saijun He,Hongmei Cao,Benchao Su,Tianzeng Huang,Qi Chen,Minghuan Liu,Da-Peng Yang
A novel ultrasensitive electrochemiluminescence (ECL) immunoassay based on Au/CaCO3 was proposed for detecting ochratoxin A (OTA) in coffee. Au/CaCO3 nanocomposites synthesized using waste eggshells as the template with a large surface area and excellent electrochemical properties were applied for immobilizing a large amount of Ru(bpy)32+ and conjugating a high-affinity nanobody (prepared by the phage display technique). Coupling of the Au/CaCO3 nanocomposites and nanobody technologies provided an ultrasensitive and highly selective ECL immunosensor for OTA detection in the range of 10 pg/mL–100 ng/mL with a low detection limit of 5.7 pg/mL. Moreover, the as-prepared ECL immunosensor showed excellent performance and high stability. Finally, the proposed ECL sensor was applied to analyze OTA in coffee samples, confirming the desirable accuracy and practical applicability potential. Overall, this work presents a new nanomaterial for fabricating the sensing interface of immunosensors by harnessing natural waste as the source and a method for detecting toxic OTA in foods.
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高影响因子
BIOSENSORS & BIOELECTRONICS
·
2021
·
IF 10.618
Xiaolei Song,Qin Ding,Yuepu Pu,Juan Zhang,Rongli Sun,Lihong Yin,Wei Wei,Songqin Liu
Ochratoxin A (OTA) is one of the most toxic mycotoxins that exists in various agro-products and foods. Here, a non-label and enzyme-free fluorescence biosensor for highly specific detection of OTA has been developed by the combination of toehold-mediated strand displacement reaction (TMSD) and G-quadruplex dimer/ThT (G-dimer/ThT). The DNA duplex (aptamer-IP) is composed of the anti-OTA aptamer and a single stranded initiation probe (IP). In the presence of OTA, the attachment of target to aptamer leads to the liberation of the IP, which activates the cycle TMSD amplifications of two hairpin probes (H1 and H2) accompanied by the production of numerous H1–H2 assemblies. This double-stranded H1–H2 structure results in the proximity between the 5′-end overhang tail of H1 and the 3′-end stem of H2 to liberate the pre-blocked G-dimer sequence for lighting up ThT . In addition, the method displayed a stable fluorescence emission in the high-salt media. It was successfully applied to analyze OTA in real food samples. Hence, the constructed fluorescence biosensing platform might provide a new way for OTA and other toxin analysis detection.
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高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2020
·Vol. 101
·期 4
·1659-1665
·
IF 2.614
Yuhao Mao,Mingna Sun,Xia Hong,Sagnik Chakraborty,Jinsheng Duan,Ming Li,Daolin Du
BACKGROUND The toxicity and health risks of mycotoxins have encouraged increased awareness and strict monitoring of these contaminants in agricultural by-products. In this paper, we developed and tested a sensitive, selective, and self-assembling immunochromatographic (IC) strip for on-site detection of ochratoxin A (OTA). We were able to demonstrate semi-quantitative and quantitative detection of OTA in agricultural by-product samples. RESULTS The optimized IC strip had a limit of detection (LOD) of 0.5 ng mL −1 OTA using the naked eye for semi-quantitative detection. When a digitized strip reader was used to achieve quantitative results, the LOD for OTA was reduced to 0.1 ng mL −1 with a linear detection range of 0.1–10 ng mL −1 . We also evaluated the specificity, stability, accuracy, and precision of the IC strip and demonstrated high performance in all of these areas. We then confirmed the ability of the IC strip to visually detect OTA contamination in authentic agricultural by-product samples from the markets in China. Furthermore, quantitative detection of OTA using the IC strip showed that the concentration of OTA ranged from 1.7 to 101.3 ng g −1 in the positive agricultural by-product samples, correlating well with the measurements obtained via liquid chromatography with tandem mass spectrometry. CONCLUSION The results indicated that this proposed IC strip was capable of sensitive, semi-quantitative, quantitative, and on-site detection of OTA contamination in agricultural by-product samples. © 2020 Society of Chemical Industry
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ACS Applied Bio Materials
·
2020
·
IF 0
Xiaohui Jing,Lin Chang,Lixia Shi,Xiaohong Liu,Yuan Zhao,Wei Zhang
As a powerful toxin that could cause fatal death, the detection of ochratoxin A (OTA) has gained much attention in the fields of environmental and food sciences. In this study, an internal standard (IS) aptasensor was synthesized through a facile and scalable method to enhance the sensitivity and quantativity of OTA detection. The substrates were formed through hybridization of modified aptamers on Au@Ag core–shell nanoparticles (NPs) and Au films at a silicon surface. Incorporated with 4-ATP and 4-NTP as an internal standard, OTA recognition of such aptamers could cause NP release and signal losses. Utilizing the strong peaks at 1078 and 1335 cm–1, which represent 4-ATP and 4-NTP, respectively, the intensity ratio of I1078/I1335 could delegate the OTA concentration in a ratiometric manner. Therefore, the highest ratio of I1078/I1335 represents the lowest concentration of OTA, and a lower ratio means a higher OTA concentration. Quantitatively, the high consistency for OTA detection was achieved through correction of signal losses by IS references with an R2 of 0.993 and RSD of 0.94%, and the OTA detection limit of 5 pM was achieved. Herein, such an IS aptasensor provides a reliable and scalable detection platform for various molecules in a continuous and high-throughput manner and holds great promise in future quantitative SERS measurements.
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FOOD AND AGRICULTURAL IMMUNOLOGY
·
2020
·
IF 0
Yuan Cheng,Liqiang Liu,Haiying Liu,Liguang Xu,Hua Kuang
In this study, a high sensitivity and specificity monoclonal antibody (mAb) was developed based on the hydroxy and carboxy-termini of ochratoxin A (OTA). A fluorescent microsphere immunochromatographic test strip (FM-ICTS) was assembled for the rapid detection the OTA in rice flour. Some relevant parameters were optimized including the best concentration of coating antigen and optimal diluent buffer composition. Under optimal conditions, the strip assay had a visual limit of detection (LOD) of 2 ng/mL and a cut-off value of 16 ng/mL for OTA in rice flour samples. By means of a strip scan reader, we obtained a quantitative measurement for OTA with an LOD of 1.27 ng/mL. No significant difference in the strip test results was seen when compared to an ic-ELISA assay or LC–MS/MS. This also shows, therefore, that the FM-ICTS has potential application for rapid and accurate quantitative detection in the market place.
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