Scientific Reports
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2021
·Vol. 11
·期 1
·1-12
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IF 4.38
Yang Chenghu,Miao Shichao,Li Tiejun
The influences of water washing treatment on the properties of Ulva prolifera -derived biochar ( U.P- biochar) and its sorption characteristics of ofloxacin (OFL) were investigated. The results showed that the water washing treatment significantly changed the physiochemical structures of U.P- biochars, and improved the sorption capacity of OFL. The sorption capacity of OFL by U.P -biochar was closely dependent on pyrolysis temperature (200–600 °C) and equilibrium solution pH (3–11). Different sorption mechanisms (e.g. cation exchange, electrostatic attraction, H-bond and cationic–π and π–π interactions) were dominant for specific U.P- biochars under various pH regions (acidic, neutral and alkaline). Moreover, the unwashed and washed U.P- biochars prepared at 200 °C (BC200 and BCW200) showed a higher sorption capacity of OFL at pH = 7. The two-compartment first-order model provided an appropriate description of the sorption kinetics of OFL by BC200 and BCW200 ( R 2 > 0.98), which revealed that the contribution ratios between the fast and slow sorption compartments ( f fast /f slow , 1.55 for BC200 and 1.25 for BCW200) reduced after water washing treatment of U.P -biochar. The values of n for the Freundlich model were less than 1, which demonstrated that the sorption of OFL by BC200 and BCW200 was favourable and nonlinear. Also, the sorption of OFL by BC200 and BCW200 increased with an increase in solution temperature and the sorption process was spontaneous and endothermic. This study provides valuable information for being a primary consideration in the production and application of U.P- biochar.
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FOOD AND AGRICULTURAL IMMUNOLOGY
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2017
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IF 1.392
Xiujin Chen,Zhaozhou Li,Jinying Guo,Daomin Li,Hongli Gao,Yao Wang,Chuanlai Xu
A broad-specificity immunoassay was established to monitor marbofloxacin (MBF) and ofloxacin (OFX) residues in raw milk and porcine muscle samples. The main influential parameters in the assay buffer were investigated to improve the assay performance. The cross-reactivities (CRs) were lower than 0.25%, except for OFX (41.38%). The quantitative working ranges for MBF and OFX based on fortified matrices ranged from 0.11 to 30.85 ng mL−1, with a 50% inhibitory concentration of 0.76±0.12 ng mL−1 for MBF and 2.70±0.28 ng mL−1 for OFX. The detection limits of enzyme-linked immunosorbent assay (ELISA) ranged from 0.053 to 0.358 ng mL−1 in spiked samples. The mean recoveries were in the range of 80.52–95.46%, less than 12.06% of the coefficient of variation. A good correlation between the ELISA results and those from instrument methods demonstrated the practical application of the novel ELISA. Therefore, the immunoassay proposed is feasible for MBF and OFX analysis in animal-derived foods.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2024
·
IF 4
Can Li,Shuangshuang Cui,Jian Zhang,Yunfeng Zhao,Xiayu Peng,Fengxia Sun
In this paper, Prussian blue nanoparticles (PBNPs) and colloidal gold (AuNPs) were synthesized by solvothermal method and trisodium citrate reduction method respectively, and two lateral flow immunoassay (LFIA) methods for the detection of ofloxacin (OFL) in fish were established. The larger particle size and superior optical properties of PBNPs make the sensitivity of PBNPs-LFIA higher than that of AuNPs-LFIA. In the process of establishing the assay, we found that the amount of antibody required for PBNPs-LFIA is 1/3 of that for AuNPs-LFIA, and the amount of probe is 1/2 of that for AuNPs-LFIA, suggesting that PBNPs-LFIA is able to save the amount of antibody and greatly reduce the cost of the assay.The detection limit of PBNPs-LFIA for OFL was 0.064 ng mL -1 , which was 2.3 times lower than that of AuNPs-LFIA, and the visual detection limit and elimination value of PBNPs-LFIA were also lower than that of AuNPs-LFIA.In addition, the recoveries of two established LFIAs for the determination of OFL in fish were 84.8%-116.3%, and the coefficient of variation was less than 10%. Therefore, PBNPs are a potential LFIA probe marker.
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JOURNAL OF HAZARDOUS MATERIALS
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2023
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IF 13.6
Mingjian Liu,Xiaowen Yu,Mengyao Yang,Wangxinze Shu,Furong Cao,Qian Liu,Jun Wang,Yong Jiang
Nanoplastic is increasing in environments and can address toxic effects on various organisms. Particle size, concentration, and surface functionalization most influence nanoplastic toxicity. Besides, nanoplastic can adsorb other contaminants (e.g., antibiotics) to aggravate its adverse effects. The combined effects of nanoplastics and antibiotics on planktonic/benthic microbial communities , however, are still largely unknown. In this study, the combined effects of polystyrene nanoplastic and ofloxacin on the structure, assembly, and metabolic activities of marine microbial communities were investigated based on amplicon sequencing data. The results mainly demonstrate that: (1) nanoplastic and ofloxacin have greater impacts on prokaryotic communities than eukaryotic ones; (2) niche breadths of planktonic prokaryotes and benthic eukaryotes were shrank with both high nanoplastic and ofloxacin concentrations; (3) increased ofloxacin mainly reduces nodes/edges of co-occurrence networks, while nanoplastic centralizes network modularity; (4) increased nanoplastic under high ofloxacin concentration induces more differential prokaryotic pathways in planktonic communities, while benthic communities are less influenced. The present work indicates that co-presence of nanoplastics and ofloxacin has synergistic combined effects on community structure shifts, niche breadth shrinking, network simplifying, and differential prokaryotic pathways inducing in marine microbial communities, suggesting nanoplastics and its combined impacts with other pollutions should be paid with more concerns.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2019
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IF 2.931
Zhiyu Yan,Haoyang Yi,Lumei Wang,Xiaotong Zhou,Rui Yan,Dongwei Zhang,Song Wang,Lantian Su,Shanshan Zhou
This paper proposes the idea of building a fluorescent biosensor for ofloxacin (OFL) determination in aqueous and milk samples by label–free OFL–specific aptamer, gold nanoparticles (AuNPs) and Rhodamine B (RB). In the absence of OFL, AuNPs are coated with OFL aptamer and maintain dispersed in the high concentration of NaCl. The dispersed AuNPs could reduce the strong fluorescence intensity of RB efficiently. By contrast, in the presence of OFL, OFL is combined with aptamer to form stable compounds, causing the aptamers separated from the surface of AuNPs, thus AuNPs would be exposed in the solution. And the aggregated AuNPs will not quench the fluorescence intensity of RB. Through the distinction of the fluorescence intensity, the concentration of OFL could be detected in aqueous and milk samples quantitatively. The convenient and specific fluorescent assay for OFL is established with a linear range ( R = 0.9907) from 20 to 300 nM and a detection limit of 1.66 nM in aqueous solution, and a linear range ( R = 0.9963) from 20 to 300 nM and a detection limit of 4.61 nM (1.66 μg/L) in milk samples. With the good sensitivity and selectivity , this biosensor has good application potential to detect OFL in food and environmental samples.
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CHEMICAL ENGINEERING JOURNAL
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2023
·
IF 15.1
Zhiyan Liu,Na Tian,Zhongyu Zhang,Nengneng Li,Yulun Nie,Xike Tian,Chu Dai
The nano zero-valent iron (nZVI)/peroxymonosulfate (PMS)-based advanced oxidation processes (AOPs) are promising for removal of antibiotic contaminants in groundwater. However, the inherent defects of rapid passivation and inactivation limit the efficient and steady application of nZVI. Herein, the lanthanum@nZVI/rGO (La@nZVI/rGO) was synthesized in which nZVI was coated with La and distributed evenly on reduced graphene oxide (rGO) matrix. The introduction of La coating protected the nZVI from direct contact with PMS molecules thus the La@nZVI/rGO appeared efficient and steady activization of PMS to degrade ofloxacin (OFX) without rapid inactivation. The removal rate was promoted to 96.5 % compared with the uncoated nZVI/rGO (only 85.2 %). The degradation reaction represented steady and continuous activation of PMS with pseudo-first-order kinetics (0.0451 min −1 ). While the two-stage kinetics of nZVI/rGO/PMS with initial 0.2581 min −1 and instant 0.0024 min −1 resulted from the rapid inactivation of nZVI. The reaction of La@nZVI/rGO/PMS exhibited minimal leaching of Fe ions (less than 1.8 µg/L) while it reached 6.4 mg/L in nZVI/rGO/PMS. XRD and XPS analyses further demonstrated the excellent durability of La@nZVI/rGO and the obvious passivation of nZVI/rGO. ESR and quenching tests confirmed that La coating regulated the PMS activation and ROS generation mechanism. The degradation was dominated by 1 O 2 -induced non-free radical process in La@nZVI/rGO/PMS system and different from radical oxidation in nZVI/rGO/PMS. Moreover, the dissolved oxygen and XPS analysis revealed the important role of La coating and oxygen vacancy in the catalytic degradation. This work could provide a novel understanding of the nZVI with protective coating in AOPs environmental remediation.
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ANALYTICAL CHEMISTRY
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2026
·
IF 6.7
Xue Yang,Yao Pan,NaiSheng Wu,Huiqing Ding,Fangling Wu,Li Ding,Chuan-Fan Ding
The precise analysis of chiral drugs is crucial for elucidating their stereoselective pharmacological effects. As a commonly used fluoroquinolone antibiotic, ofloxacin (OFLX) exhibits different pharmacodynamics between its R- and S-OFLX, highlighting the need for efficient chiral analysis methods. This study developed a strategy based on trapped ion mobility spectrometry-mass spectrometry (TIMS-MS) and noncovalent complex formation for the rapid separation and quantification of ofloxacin enantiomers. Vancomycin and gentamicin C1a were selected as chiral selectors to form binary noncovalent complexes with the target enantiomers. TIMS analysis demonstrated that the complexes formed between R-/S-OFLX and both chiral selectors achieved baseline separation, with peak-to-peak resolution values of 1.94 and 3.34, respectively. To clarify the structural basis of chiral recognition, density functional theory calculations and the independent gradient model were employed, revealing key stereospecific weak interactions within the complexes, thereby providing critical atomic-level insight into the differential migration behavior observed in TIMS experiments at the molecular level. Based on these findings, both relative and absolute quantification methods for the enantiomers were established, both demonstrating excellent linearity and high sensitivity. Finally, the method was successfully applied to determine ofloxacin in artificial urine samples, achieving recoveries of no less than 70.60%, confirming its applicability in complex real-world matrices. This derivatization-free approach provides a robust and efficient solution for the rapid analysis of chiral drugs.
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INORGANIC CHEMISTRY COMMUNICATIONS
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2026
·
IF 5.9
Yue Li,Ping Liu,Xiaoxue Han,Longyu Guo,Haiyan Jiang,Yongchao Ma
A heterojunction with g-C 3 N 4 @Zn/Eu-MOF covalent interface was synthesized. • g-CN@MOF can be used for photocatalytic degradation and fluorescence detection of ofloxacin. • Efficient removal of ofloxacin in milk by g-CN@MOF foam. • g-CN@MOF can detect ofloxacin residues in milk through smartphone-assisted color recognition. Ofloxacin (OFL) is widely used in medicine and animal husbandry, and its residues in milk raise substantial food-safety concerns. Herein, a dual-functional covalently coupled g-C 3 N 4 @Zn/Eu-MOF composite (g-CN@MOF) was constructed through a one-pot hydrothermal route. FTIR and XPS analyses indicated the formation of C N C O linkages between g-C 3 N 4 and the MOF, yielding an interfacially coupled type-II heterojunction that promoted charge separation. The optimized g-CN@MOF-30 achieved 69.21% removal of 10 mg L −1 OFL within 120 min under visible light, with •O 2 − and •OH identified as the dominant reactive species. Meanwhile, the fluorescence intensity ratio F 440 /F 595 showed a linear response to OFL over the range of 0–100 μM, affording a detection limit of 0.011 μM through an IFE-dominated ratiometric sensing mechanism. After immobilization in sodium alginate foam, the composite exhibited convenient recyclability and enabled OFL removal from pretreated milk samples, while smartphone-based RGB analysis provided rapid visual semi-quantification with recoveries ranging from 100.3% to 109.9%. Download: Download high-res image (269KB) Download: Download full-size image
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CHEMICAL ENGINEERING JOURNAL
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2023
·
IF 15.1
Bo Li,Huan-Yan Xu,Yu-Long Liu,Yue Liu,Yan Xu,Si-Qun Zhang
As a fluoroquinolone antimicrobial agent, ofloxacin (OFX) has been widely used, consequently causing serious damage to the ecological environment. Advanced oxidation processes based on persulfate activation (SR-AOPs) are widely applied to degrade stubborn organic contaminants in wastewater treatment. Co 3 O 4 is being extensively investigated as a promising persulfate catalyst in SR-AOPs. In this work, four Co 3 O 4 polycrystals with specific morphologies were synthesized and used to activate peroxymonosulfate (PMS) for OFX degradation. The obtained samples and corresponding precursors were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), and N 2 adsorption/desorption isotherms. Experimental results showed that 30 mg·L -1 OFX could be completely removed in the lamella-like Co 3 O 4 /PMS system within 30 min under ordinary conditions. The underlying degradation mechanism was elaborated by high-resolution TEM (HRTEM) combined with density functional theory (DFT) calculations. Based on quenching experiments and the electron paramagnetic resonance (EPR) technique, sulfate radicals were identified as the dominant reactive oxygen species (ROS) in the lamella-like Co 3 O 4 /PMS system, and superoxide radicals, hydroxyl radicals, and singlet oxygen made minor contributions to the degradation of OFX. Possible degradation pathways were proposed based on DFT calculations and Fukui theory and confirmed by ultrahigh-performance liquid chromatography-quadrupole-time of flight mass spectrometry (UHPLC-QTOF/MS). Furthermore, quantitative structure–activity relationship (QSAR) prediction was used to evaluate the developmental toxicity of the corresponding intermediates.
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ENVIRONMENTAL TECHNOLOGY
·
2022
·
IF 3.475
Ruobing Yu,Zhicong Wu
Although metal–organic frameworks (MOFs) are advantageous to the removal of organic pollutants, the general MOFs in powder form is disadvantageous to their practical applications. In-situ MOF synthesis in alginate gel is a good way to fabricate an MOF composite for many applications, which is different from blending MOF particles with polymers. In-situ synthesis of Zeolitic Imidazolate Framework-8 (ZIF-8) in alginate gel is in the form of beads with rough wrinkles and has many pores inside. When used as an absorbent, in-situ synthesis of ZIF-8 in alginate gel could remove 97.7 ± 0.9% of ofloxacin from ofloxacin solution and the equilibrium adsorption capacity is up to 160.6 ± 1.3 mg/g. During the adsorption, ofloxacin is first brought into the gel by the solvent exchange and gel microchannel adsorption, and it can then be absorbed by in-situ ZIF-8. Moreover, the adsorption efficiency can reach 85.5% even after four cycles of adsorption. We believe that in-situ synthesis of ZIF-8 in alginate gel will be an appropriate material for the removal of ofloxacin in the wastewater.
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Nano Research
·
2020
·Vol. 13
·期 9
·2340-2350
·
IF 8.183
Huang Yue,Gao Qiang,Li Xu,Gao Yifan,Han Haijie,Jin Qiao,Yao Ke,Ji Jian
Antibiotic resistance is an increasingly serious threat to global public health, which can lead to the decrease of the effectiveness of antibiotics. The combination therapy of antibiotic and mild temperature photothermal therapy (PTT) is adopted to address this issue in this work. An antibiotic-loaded nanoplatform is fabricated based on two-dimensional (2D) molybdenum disulfide (MoS 2 ) nanoflakes as effective near-infrared (NIR) photothermal agent. The MoS 2 nanoflakes is modified with positively charged quaternized chitosan (QCS) to improve the dispersion stability and enhance the interaction between MoS 2 nanoflakes and bacterial membrane. The QCS modified MoS 2 nanoflakes (QCS-MoS 2 ) is expected to adhere onto the membrane of methicillin-resistant Staphylococcus aureus (MRSA) and depolarize the bacterial membrane by local hyperthermia under NIR irradiation. A first-line antibiotic, ofloxacin (OFLX), can be loaded onto QCS-MoS 2 by π-π stacking and hydrophobic interaction. Due to the combined antibiotic-photothermal therapy, superior bactericidal ability was achieved at mild temperature (45 °C) and low antibiotic concentration. Such synergistic mild-temperature photothermal/antibiotic therapy can not only avoid the damage to neighboring tissue by PTT, but also reduce the development of drug resistance, providing an innovative way for the treatment of bacterial infections.
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Materials Science & Engineering C-Materials for Biological Applications
·
2019
·
IF 4.959
Ping Zhang,Tong Lu,Xiaojie Xia,Lingqiong Wu,Long Shao,Jiahua Zhou,Jing Li
The purpose of this study was to facilely develop biomimetic amino modified mesoporous silica xerogel (AMSX) and study how AMSX regulated loading and in vitro sustained delivery of carboxyl-containing drug levorotary ofloxacin (LOFL). Characteristics of AMSX, including morphology, porous structure, elements and crystalline state were investigated and pharmaceutical performance of AMSX for the delivery of LOFL was studied. The result showed that AMSX was accumulational spherical nanoparticles with mesoporous structure. Hydrogen bonding force was formed between carboxylic groups of LOFL and amino groups grafted on the surface of AMSX. Furthermore, a three-level three-factorial Box–Behnken experimental design was applied to optimize the amount of major agent for synthesizing AMSX with expected drug loading capacity and also to figure out how AMSX regulated in vitro delivery of LOFL. It is believed that the present work will provide novel insights for designing mesoporous silica as drug carrier and favored the development of sustained release system.
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