ANALYST
·
2018
·Vol. 143
·期 15
·3702-3707
·
IF 3.864
Jun-Tao Cao,Fu-Rao Liu,Fang Hou,Juan Peng,Shu-Wei Ren,Yan-Ming Liu
The cathodic electrochemiluminescence (ECL) behaviour of nontoxic MoS2 quantum dots (QDs) was studied for the first time using potassium peroxydisulfate as the co-reactant. Ag-PAMAM NCs, serving as difunctional tags for quenching and enhancing ECL of MoS2-reduced graphene oxide composites, were introduced into the ECL detection system for signal amplification. By modulating the interparticle distance between MoS2 QDs and Ag-PAMAM NCs, the ECL quenching from resonance energy transfer and the ECL enhancement from surface plasma resonance were realized. Coupling the good ECL performance of MoS2 QDs with the excellent ECL quenching and enhancement effects of Ag-PAMAM NCs, a novel MoS2 QDs-based ECL biosensing platform for sensitive detection of microRNA-21 was achieved with a detection limit of 0.20 fmol L−1 (S/N = 3). This method was successfully applied to the determination of microRNA-21 in human serum samples with recoveries of 90.0–110.0%, suggesting great potential for its applications in biological and chemical analysis.
研究领域:
分析化学
·
生物化学
·
纳米技术
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
Nanomedicine-Nanotechnology Biology and Medicine
·
2017
·
IF 5.72
Zhichun Shen,Bowen Li,Yajun Liu,Guirong Zheng,Yan Guo,Ruirui Zhao,Kai Jiang,Lulu Fan,Jingwei Shao
Ursolic acid (UA), a natural triterpene acid, is a promising anti-cancer drug due to its inhibitory effect on tumor growth and metastasis. However, clinical translation of UA is limited by its poor water solubility and low bioavailability. To overcome these problems, herein an amphiphilic self-assembly nanodrug composed of UA, lactobionic acid (LA) and low-polyamidoamine (low-PAMAM) dendrimers is developed. This near-spherical nanodrug with a uniform size (~180 nm) demonstrated to have an enhanced cytotoxicity against liver cancer SMMC7721 cells, and could attenuate the migration and adhesion of SMMC7721 cells at non-toxic concentrations by suppressing metastasis-related protein MMP-9 expression. Furthermore, in vivo study indicates that the nanodrug exhibited prolonged circulation time in blood as well as increased AUC, MRT and C max , and could effectively inhibit the tumor growth in H22 mice model. Overall, the UA-based nanodrug delivery system reported in the present work represents a novel strategy for targeted tumor therapy.
研究领域:
药学
·
纳米技术
·
肿瘤学
·
药物递送系统
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产品关联
DOI
PubMed
高影响因子
Sensor Letters
·
2016
·Vol. 14
·期 6
·623-629
·
IF 0
Li Liping,Liu Xiaojia,Xiao Qian,Zheng Yizhi,Wang Suiping,Feng Bo
A novel antibody immobilization method was developed for enzyme-linked immunosorbent assay (ELISA). The proposed method utilizes amine-bearing polymer macromolecules, namely, polyamidoamine (PAMAM) or polyethylenimine (PEI) to enhance antibody binding on polystyrene (PS) microwells,
as well as staphylococcal protein A (SPA) to realize orientation immobilization. After carboxylating the PS microwells with the solution of potassium permanganate (KMnO4) in diluted sulfuric acid (H2SO4), the PS surface was activated by 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide
hydrochloride and N-hydroxysuccinimide and then grafted with PAMAM or PEI to introduce free amino groups. Glutaraldehyde was subsequently added to conjugate the amino groups and SPA prior to antibody adsorption. X-ray photoelectron spectroscopy and atomic force microscopy were performed to
investigate the morphology of the PS surfaces before and after different treatments. The immunoglobulin binding sites were confirmed through anti-Fab and anti-Fc ELISA kits. Results from the alpha fetoprotein ELISA kit showed that the use of both PAMAM and SPA is the optimal method for antibody
immobilization. In this treatment, absorbance exhibited an evident gradient with antigen concentrations and reached higher values over 200 ng/mL antigen compared with the ones of other methods.
研究领域:
生物化学
·
分子生物学
·
分析化学
✓ 已验证
DOI
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2013
·
IF 2.108
Pei Zhang,Hui Wang,Xia Liu,Xuefeng Shi,Jian Zhang,Guang Yang,Keji Sun,Jinben Wang
The dynamic surface tension and dilational viscoelasticity properties of amphiphilic polyamidoamine (PAMAM) dendrimers were investigated to probe the hydrophobic chain effect on the interfacial properties and the demulsification behaviors. The values of dynamic parameters n and t * representing diffusion speed of the molecules obtained according to the Rosen equation decreased with the increasing of bulk concentration. Taking the hydrophobic effect into consideration, the t * values decreased and n values increased with the increase of alkyl chain length, suggesting easier adsorption and faster diffusion for longer hydrophobic chain which was opposite to the tendency of traditional surfactants. Further dilational viscoelasticity studies demonstrated that the properties referring to adsorption and exchange-diffusion were probably affected by the aggregates conformation at the interface. Subsequently, from the demulsification experiments a conclusion can be drawn that the amphiphilic dendrimers with branched dendritic structure possesses superior properties of breaking W/O crude oil emulsions, showing a certain correlation to the dilational viscoelasticity properties.
研究领域:
胶体与界面化学
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石油化学
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高分子科学
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流变学
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表面活性剂化学
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Xin Ding,Ya Zhang,Qian Zhou,Guoyin Zheng,Yan Qiao,Yang Zhang,Junli Liang,Hongrui Ma,Xiaofei Chen,Guangguo Tan
Radix Aconiti Lateralis Preparata (Fuzi in Chinese) is an efficient Chinese herbal medicine (CHM) against doxorubicin (DOX)-induced cardiomyopathy (DIC), but the cardioprotective compounds and targets of Fuzi remain unclear. In this work, a novel two-dimensional (2D) polyamidoamine (PAMAM)-modified cardiac mitochondrial membrane chromatography (CMMC) system, with the characteristic of high-density mitochondrial membrane coating and longer column lifespan, was developed for the identification of bioactive components from Fuzi to counteract DIC. Three compounds with strong retention were screened out to have cardioprotective effects against DOX-induced cardiotoxicity in different cardiomyocytes, and one promising compound songorine with the highest affinity was selected for further in vitro and in vivo pharmacodynamic validation and target identification. Hadha, involved in fatty acid oxidation (FAO) and apoptosis, was identified as the functional target of songorine by cardiac mitochondria proteomics, drug affinity responsive target stability (DARTS), cellular thermal shift assay (CETSA), surface plasmon resonance (SPR) and molecular docking assays. siRNA experiments have also demonstrated that songorine can affect the function of Hadha. It was concluded that songorine could directly bind to the Hadha protein to alleviate the functional impairment of the mitochondrial FAO and oxidative phosphorylation (OXPHOS) and regulate the Bcl-2 family proteins in mice to treat DIC.
研究领域:
药理学
·
天然产物化学
·
线粒体生物学
·
心血管研究
·
中医药学
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药物发现
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蛋白质组学
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2015
·Vol. 7
·期 4
·1471-1477
·
IF 1.821
Siyuan Wang,Wen Zhang,Xia Zhong,Yaqin Chai,Ruo Yuan
A nanohybrid based on reduced graphene oxide functionalized by poly(amido-amine), multi-walled carbon nanotubes and Au nanoparticles (RGO–PAMAM–MWCNT–AuNPs) for simultaneous electrochemical determination of ascorbic acid (AA), dopamine (DA) and uric acid (UA) was reported in this paper. The RGO–PAMAM–MWCNT–AuNP-modified electrode showed a high selectivity towards the oxidation of AA, DA, and UA, and resolved their overlapped oxidation peaks into three well-defined peaks. The RGO–PAMAM–MWCNT–AuNP nanohybrids were characterized by scanning electron microscopy (SEM). Several important parameters that control the performance of the electrochemical sensor were investigated and optimized. Under optimal conditions by a differential pulse voltammetry (DPV) method, the linear response ranges for the determination of AA, DA, and UA are 20 μM to 1.8 mM, 10 μM to 0.32 mM, and 1 μM to 0.114 mM in the co-existence systems, respectively. The corresponding detection limits are 6.7 μM, 3.3 μM and 0.33 μM (S/N = 3), respectively.
研究领域:
分析化学
·
纳米材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Nano Materials
·
2023
·
IF 5.9
Fangdi Wei,Yuan Yao,Qiutong Chen,Jiamei Song,Zhen Xie,Li Ai,Guanhong Xu,Yao Cen,Yuhui Lin,Jing Yang,Qin Hu,Rui Li
Inspired by the distinctive merits of carbon nanoparticles (CNPs) and poly(amidoamine) (PAMAM) dendrimers, we synthesized PAMAM modified CNPs (CNPs-PAMAM). First, CNPs were prepared using citrate acid and 1, 2, 4-triaminobenzene. Then, PAMAM was anchored on the surface of CNPs. The structure of PAMAM would remain unchanged under mild conditions of the postmodification method, making PAMAM retain its excellent drug carrying ability. Particularly, CNPs-PAMAM had exceptional optical properties of excitation-independent long wavelength emission. Excitation-independent emission can ensure the sensitivity of bioimaging. Long wavelength emission can avoid the autofluorescence interference of biological matrixes and have good tissue penetration. Further studies demonstrated that CNPs-PAMAM were able to electrostatically bind and condense small interfering RNA (siRNA) into stabilized nanocomplexes. The siRNA complexed with CNPs-PAMAM was endowed with an increased resistance against degradation and an enhanced cellular uptake. Moreover, CNPs-PAMAM-complexed siRNA targeting histone deacetylase 2 (HDAC2) could be effectively delivered into the neuron-like PC-12 cells, and exert its gene-silencing effect. Precisely inhibiting the so-called undruggable target HDAC2 opens a novel strategy for ischemic stroke treatment. Thus, CNPs-PAMAM offer a promising delivery carrier to unleash the siRNA’s therapeutic potential.
研究领域:
纳米技术
·
生物医学工程
·
分子生物学
·
药学
✓ 已验证
产品关联
DOI
高影响因子
BioNanoScience
·
2025
·Vol. 15
·期 3
·1-17
·
IF 3.2
Lu Tianwen,Dai Yixuan,Shi Yue,Wang Xiaojun,Hong Yinan,Pan Hong,Wu Zhansheng
Laccase is a kind of green biocatalyst which is widely distributed in nature. However, the application of free laccase was greatly limited due to the poor stability and low utilization. In order to prepare a carrier with abundant immobilized sites, This study designed a novel magnetic dendrimers (MPG-x) as an excellent carrier for immobilizing laccase. Fe 3 O 4 was used as the core, and the desired MPG-x was obtained by modifying the surface of Fe 3 O 4 with hyperbranched polyaminoamide (PAMAM) dendrimers. Due to its controllable generation number, this carrier can provide a relatively abundant number of surface binding sites for laccase immobilization. Then the immobilized laccase MPG-2@GA-Lac and MPG-2@Cu 2+ -Lac were prepared by glutaraldehyde crosslinking and copper chelating adsorption, respectively. The results showed that the loading of MPG-2@GA-Lac and MPG-2@Cu 2+ -Lac reached 48.9 mg/g and 50.6 mg/g respectively, and MPG-2@GA-Lac and MPG-2@Cu 2+ -Lac retained 74.4% and 108.1% of the initial free laccase activity, respectively. In addition, the two immobilized laccase showed better pH stability, temperature stability, thermal stability and storage stability than free laccase. The activation of Cu 2+ in the active center of laccase made MPG-2@Cu 2+ -Lac exhibit better stability. Methyl orange (MO) removal experiments showed that MPG-2@Cu 2+ -Lac exhibited higher MO removal efficiency (82.56%) over a wider pH and temperature range than MPG-2@GA-Lac (72.19%), and MPG-2@Cu 2+ -Lac maintained a MO removal efficiency of 66.18% after 6 cycles of operation. This study is expected to further expand the application of laccase in the degradation of dye wastewater.
研究领域:
环境科学
·
生物技术
·
纳米技术
·
酶工程
·
废水处理
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Yan Xu,Zhenbo Wang,Caifeng Ding,Xiliang Luo
It is a great challenge to detect the target selectively and accurately in complex biological samples. A ratiometric signal amplification strategy based on antifouling peptide is proposed to detect thrombin (TB) in this work. The electrode is modified with conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) and [email protected] Then the bi-functional peptide anchors to the modified electrode and captures the low toxic electrochemiluminescence (ECL) probe. The bi-functional peptide includes a new antifouling sequence (DRDRDRDR) and a TB recognizable sequence (SGRPVLG), while the former can greatly reduce the non-specific adsorption in complex samples and the latter can be cleaved by TB. The new self-enhanced luminescence probe labeled as PAMAM-QDs is composed of low toxic and water-soluble CuInZnS/ZnS quantum dots (CIZS/ZnS QDs) and polyamidoamine (PAMAM) via amide bonds. Concentration of TB can be obtained via the ratio of the signals of ECL QDs /ECL [email protected] accurately and sensitively. The ECL biosensor with the detection line 1.82 fM can work from 10 fM to 1 nM. Thanks to the antifouling property of the designed peptide and the amplification of the new ECL probe, the biosensor can be used for quantifying TB in practical complex samples with high selectivity and sensitivity.
研究领域:
分析化学
·
生物传感器
·
纳米技术
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
BIOMACROMOLECULES
·
2022
·
IF 6.978
Zhiyu Yang,Lin Lin,Zhaopei Guo,Xiaoya Guo,Zhaohui Tang,Huayu Tian,Xuesi Chen
The relatively low transfection efficiency limits further application of polymeric gene carriers. It is imperative to exploit a universal and simple strategy to enhance the gene transfection efficiency of polymeric gene carriers. Herein, we prepared a cationic polypeptide poly(γ-aminoethylthiopropyl-l-glutamate) (PALG-MEA, termed PM) with a stable α-helical conformation, which can significantly improve the gene transfection efficiency of cationic polymers. PM can be integrated into polymeric gene delivery systems noncovalently through electrostatic interactions. With the assistance of PM, polymeric gene delivery systems exhibited excellent cellular uptake and endosomal escape, thereby enhancing transfection efficiency. The transfection enhancement effect of PM was applicable to a variety of cationic polymers such as polyethylenimine (PEI), poly-l-lysine (PLL), and polyamidoamine (PAMAM). The ternary gene delivery system PM/pshVEGF/PEI exhibited an excellent antitumor effect against the B16F10 tumor model. Moreover, we demonstrated that PM could also enhance the delivery of gene editing systems (sgRNA-Cas9 plasmids). This work provides a facile and effective strategy for constructing polymeric gene delivery systems with a high transfection efficiency.
研究领域:
遗传学
·
生物医学工程
·
分子生物学
·
基因治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Zhan Qu,Muhammad Noman Bashir,Miaomiao Wang,Yating Chen,Beenish Noureen,Zhiyao Wang,Yage Liu,Liping Du,Chunsheng Wu
Adenosine triphosphate (ATP) is crucial for cellular activity. The need for ATP detection in the field of biomedicine is on the rapid rise. Several biosensor-based approaches have been developed as a result of the growing demand for ATP detection. An electrolyte-insulator-semiconductor (EIS) sensor is a type of field-effect device that has the ability to detect surface-potential changes with a specific level of sensitivity. In this study, a label-free ATP detection biosensor that is based on poly(amidoamine)-modified EIS sensors was developed, in which ATP-sensitive aptamer (Apt) was utilized as sensitive element and EIS sensor was used as transducer. It is possible to monitor the binding of charged molecules, such as aptamers using EIS sensors in a label-free manner with a straightforward setup. To improve the coupling efficiency of Apt with EIS sensor, positively charged polyelectrolyte, i.e. poly (amidoamine) (PAMAM) dendrimers, was utilized to modify the surface of the EIS sensors to attach the negatively charged Apt based on electrostatic attraction. The adsorptive binding of Apt and ATP results in a change in capacitance of EIS sensor. During the process of surface modification, the electrochemical measurements. of capacitance-voltage (C-V) curves and constant-capacitance (ConCap) characteristics were utilized as indicators for the corresponding processes of EIS sensor surface modification. The measurements results indicated this biosensor was able to detect ATP with high sensitivity and good specificity. The detection range of ATP was 0.1. nM to 100. nM and the detection limit was as low as 0.12 nM. This biosensor has the potential to be utilized in the detection of ATP in the surrounding microenvironment of cells and tissues, which have promising prospects to be applied in the field of biomedicine such as energy and metabolism monitoring of cells and tissues.
研究领域:
生物医学工程
·
分析化学
·
生物传感器
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOELECTROCHEMISTRY
·
2022
·
IF 5.76
Shuting Cheng,Rui Xu,Fengzhen Yang,Jingcheng Huang,Xia Sun,Xue Huang,He Li,Falan Li,Yemin Guo,Mohammad Hasanzadeh,Yelong Zhu
A novel sandwich electrochemiluminescence (ECL) aptasensor was developed for highly sensitive detection of kanamycin using luminol-functionalized aptamer as a signal probe. The aptasensor used polyethyleneimine (PAMAM), molybdenum disulfide, and multi-walled carbon nanotubes as the substrate, which provided enough binding sites for aptamer1 (the aptamer which modified NH 2 ) coupling. We found that kanamycin could be detected using the aptamer1 containing the same base sequence as aptamer2 (the aptamer which modified SH) on the electrode self-assembly. In addition, PAMAM nanocomposites can be used to effectively improve the ECL intensity by loading a high volume of luminol molecules and silver nanoparticles. In the presence of kanamycin, the sandwiched aptasensor was formed between aptamer1 and the probe of aptamer2 connecting silver nanoparticles, luminol, and PAMAM, resulting in a proportional increase of ECL intensity. Since the significantly enhanced loading of luminol by PAMAM accelerated the electron transfer, the sensitive aptasensor exhibited a wide linear range of detection from 1 × 10 -3 to 1 × 10 3 ng/mL and a low detection limit of 0.21 pg/mL (S/N) for kanamycin. The fabricated aptasensor was successfully applied in quantitative analysis of kanamycin in milk samples.
研究领域:
分析化学
·
电化学
·
生物传感器
·
食品安全
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）