LUMINESCENCE
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2017
·Vol. 33
·期 2
·399-409
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IF 1.509
Zheng Chang,Yinghao Mi,Xingwang Zheng
In this paper, polyethylenimine (PEI) and Chitosan were simultaneously one-step doped into silicon dioxide (SiO 2 ) nanoparticles to synthesize PEI/Chitosan/SiO 2 composite nanoparticles. The polymer PEI contained a large amount of amino groups, which can realize the amino functionalized SiO 2 nanoparticles. And, the good pore forming effect of Chitosan was introduced into SiO 2 nanoparticles, and the resulting composite nanoparticles also had a porous structure. In pH 7.4 phosphate buffer solution (PBS), the amino groups of PEI had positive charges, and therefore the fluorescein sodium dye molecule can be loaded into the channels of PEI/Chitosan/SiO 2 composite nanoparticles by electrostatic adsorption. Furthermore, utilizing the diversity of DNA molecular conformation, we designed a high sensitive controllable assembly of DNA gated fluorescent sensor based on PEI/Chitosan/SiO 2 composite nanoparticles as loading materials. The factors affecting the sensing performance of the sensor were investigated, and the sensing mechanism was also further studied.
研究领域:
纳米技术
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材料科学
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分析化学
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生物传感器
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DOI
PubMed
高影响因子
BIOELECTROCHEMISTRY
·
2023
·
IF 5
Qin Xia,Zhaode Mu,Min Qing,Jing Zhou,Lijuan Bai
In recent decades, the incidence of breast cancer has increased year by year, posing a serious threat to human health and quality of life, and about 30% of breast cancer patients have human epidermal growth factor receptor 2 (HER2) overexpression. Therefore, HER2 has become an important biomarker and indicator for the clinical evaluation of breast cancer in diagnosis, prognosis and recurrence. In this work, polyethyleneimine functionalized MoS 2 nanoflowers (PEI-MoS 2 NFs) with good electrical conductivity and abundant active binding sites were designed and employed as a sensing platform for immobilizing the primary antibody of HER2 (Ab 1 ). In addition, a La-MOF-PbO 2 composite with a large specific surface area and good conductivity was used to load lots of electroactive toluidine blue (TB) and the secondary antibody of HER2 (Ab 2 ) via gold nanoparticles (AuNPs) as the linker. Thus, the constructed sandwich-type electrochemical immunosensor was applied for sensitive detection of HER2, which showed a wide linear range from 100 fg mL −1 to 10 μg mL −1 with a lower limit of detection of 15.64 fg mL −1 . Therefore, the resulting immunosensor in this study would have a potential application in clinical bioanalysis.
研究领域:
生物医学工程
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分析化学
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癌症研究
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电化学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 12
·1-9
·
IF 6.232
Su Zixian,Zhao Guangying,Dou Wenchao
Aflatoxin M1 (AFM1) residues in milk pose a major threat to human health, so there is an urgent need for a simple, rapid, and sensitive method for the determination of trace AFM1 in milk. In this study, a competitive immunochromatographic assay (ICA), using visual (PEI/PSS) 4 red silica nanoparticles (SiNPs) as signal amplification probes, was used for the highly sensitive detection of AFM1. The (PEI/PSS) 4 red SiNPs were used to label AFM1 monoclonal antibody (mAb) to prepare ICA for the detection of AFM1. After exploring the optimal conditions of mAb and immunoprobe dosage conditions, the lowest visual detection limit (VDL) of AFM1 in phosphate-buffered saline with Tween 20 (PBST, 10 mM, pH 7.4, containing 1% BSA, 3% sucrose, 1% trehalose, and 0.5% Tween 20) can reach 0.1 pg/mL. The intuitive visually visible value of AFM1 in both PBST and milk was 10 pg/mL. The results showed that the immunochromatographic system based on high chroma color (PEI/PSS) 4 red SiNPs has high sensitivity and broad application prospects for the detection of trace AFM1 residues in milk. The high chroma (PEI/PSS) 4 red SiNPs are expected to be a convenient biomarker for improving the sensitivity of immune chromatography bands.
The schematic diagram shows the detection principle. In this work, in the competitive experiment, (PEI/PSS) 4 red SiNPs were selected as visual labeling materials, and the specific antibody combined with the labeled material was selected as an immune probe. The AFM1-BSA antigen coupled with the macromolecular BSA was fixed on the T line of the nitrocellulose (NC) membrane. The AFM1 in sample solution competes with AFM1-BSA for the specific binding site of immune probe. The detection sensitivity of this method for AFM1 is obtained by judging the change of the red signal intensity produced by the positive sample, compared with the color at the T line of the negative sample.
研究领域:
食品安全
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分析化学
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纳米技术
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生物技术
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Qingdi Kong,Xiaojie Zhang,Kangrui Ma,Yuefa Gong,Hong Peng,Wei Qi
It is a challenging task to remove heavy metal ions efficiently from the wastewater containing high concentrations of complexants. In this work, a novel PEI-modified chitosan/activated carbon composite adsorbent (PCA) was prepared and applied to the removal of Cu(II) from pyrophosphate plating rinsing wastewater. The main species of Cu(II) in the pyrophosphate wastewater was [Cu(HP 2 O 7 ) 2 ] 4− or [Cu(P 2 O 7 ) 2 ] 6− , which were denoted as [Cu(II)-PP] anions. The maximum adsorption capacity of PCA for Cu(II) reached 1.41 mmol g −1 under the condition of pH = 8 and molar ratio of pyrophosphate to Cu(II) = 4:1. The adsorption kinetic behavior of Cu(II) on PCA followed the Elovich model best and PCA attained adsorption equilibrium within 36 h. The thermodynamic studies showed that the adsorption process of Cu(II) by PCA was endothermic and spontaneous. The PCA fixed bed column was used to remove Cu(II) from simulated pyrophosphate plating rinsing wastewater. After three consecutive adsorption-desorption cycles, the adsorption performance, hydraulic conductivity , and mechanical stability of PCA column did not decrease. The FTIR and XPS analysis results indicated that [Cu(II)-PP] anions can be adsorbed on PCA by electrostatic attraction with protonated amine groups or coordination with the amine groups of PCA via ligand substitution.
研究领域:
环境科学
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水处理
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材料科学
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化学工程
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DOI
PubMed
高影响因子
DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY
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2016
·
IF 2.429
Hailong Zhang,Xiaoyan Huang,Yongjing Zhang,Yang Gao
Context: Oral bioavailability of some hydrophilic therapeutic macromolecules was very poor, thus leading to their limited application in clinic.Objective: To investigate the efficacy, safety and mechanism of HP-β-CD-PEI polymers on the intestinal absorption of some poorly absorbable drugs in rats.Methods: Effects of HP-β-CD-PEI polymers on the intestinal absorptions of drugs were investigated by an in situ closed loop method in rats. The safety of HP-β-CD-PEI polymer was evaluated by measurement of lactate dehydrogenase (LDH) activity and amount of protein released from rat intestinal perfusate. The absorption enhancing mechanisms were explored by the measurement of zeta potential, transepithelial electrical resistance (TEER) and in vitro transport of FD4 (a paracellular marker) across rat intestinal membranes, respectively.Results: HP-β-CD-PEI polymers, especially HP-β-CD-PEI1800, demonstrated excellent absorption enhancing effects on drug absorption in a concentration-dependent manner and the enhancing effect was more efficient in the small intestine than that in the large intestine. Five percent (w/v) HP-β-CD-PEI1800 obviously decreased the TEER, accompanied with increase in the intestinal transport of FD4, indicating that absorption enhancing actions of HP-β-CD-PEI polymers were possibly performed by loosening tight junctions of intestinal epithelium cells, thereby increasing drug permeation via a paracellular pathway. A good liner relationship between absorption enhancing effects of HP-β-CD-PEI polymers and their zeta potentials suggested the contribution of positive charge on the surface of these polymers to their absorption enhancing effects.Conclusion: HP-β-CD-PEI polymers might be potential and safe absorption enhancers for improving oral delivery of poorly absorbable macromolecules including peptides and proteins.
研究领域:
药剂学
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药物递送
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药理学
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2024
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IF 4.9
Ziyan Hu,Zhongbing Huang,Juan Wang,Jirui Wen,Ximing Pu,Qianlei Lang,Peng Yang,Zhongxiang Shao,Jia Hu,Jiang Wu,Guangfu Yin
Typical symptoms of acute myocardial infarction (AMI) and acute aortic dissection (AAD) are similar chest pain, leading to higher clinical misdiagnosis rate, evenly the mortality rate of patients. In this work, ZrO 2 @PEI-QDs nanospheres with higher fluorescence were prepared by sequential electrostatic adsorption of branched PEI chains and CdSe/ZnS-COOH QDs on hollow ZrO 2 nanospheres. Three multi-channel LFIA strips (on sST2 and cTnI, cTnI and h-FABP, HMGB1 and sST2, respectively) based on ZrO 2 @PEI-QDs for rapid and correct distinguishing of AMI and ADD were constructed. Their visual limit of detection (vLOD for sST2 and cTnI, cTnI and h-FABP, HMGB1 and sST2) within 10–15 min reached 0.075 and 0.1 ng/mL, 0.1 and 1 ng/mL, 1 and 0.1 ng/mL, respectively, which were all lower than the normal level of healthy people. Compared with previous studies, ZrO 2 surface adsorbs more QDs, the fluorescence intensity was increased by about 2.84 times, and vLODs of three strips based on the quantitative fluorescence analysis were also increased by up to one orders of magnitude. The test results of five clinical serum samples indicated that three multi-channel LFIA strips could quantitatively detect four biomarkers of chest pain, and successfully distinguish AMI patients from AAD patients. These results indicated that this set of multi-channel LFIA strips could become an effective tool for the early and accurate detection or distinguish of ADD and AMI.
研究领域:
纳米技术
·
生物传感器
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医学诊断
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心脏病学
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产品关联
DOI
高影响因子
ANALYTICAL LETTERS
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2021
·
IF 2.329
Zheng Chang,Yimeng Xu,Yuting Shen
A novel sandwich-type electrochemical immunosensor was fabricated for the ultrasensitive electrochemical determination of prostate specific antigen (PSA) using silver-functionalized polyethyleneimine (PEI)-silica nanoparticles (NPs) (Ag+/PEI/SiO2 NPs) as electrochemical labels. First, the immunosensor platform was prepared by covalently immobilizing capture antibodies on a poly diallyldimethyl ammonium chloride/carboxylated-gold nanoparticles (PDDA/AuNPs) modified glassy carbon electrode (GCE). After sandwich-type immunoreactions, the detection antibody was labeled by the amino-containing Ag+/PEI/SiO2 NPs by strongly binding to carboxyl groups of protein. Next, many Ag+ with good complexing performance from each PEI/SiO2 nanoparticles released after hydrofluoric acid (HF) in situ etching. Here, the AuNPs acted as a catalyst to reduce Ag+ into metallic Ag using PEI as the reducing agent. Accordingly, an extremely sharp voltammetric peak of electro-oxidized metallic Ag can be determined directly through differential pulse voltammetry (DPV) without metal pre-concentration, and had a close relationship with the concentrations of PSA antigen. Under the optimized conditions, the immunoassay exhibited good sensitivity and selectivity for the determination of PSA with a linear range from 0.01 to 10 pg·mL−1 and a detection limit of 3.5 fg·mL−1. The new strategy provides a reliable, highly sensitive determination of the prostate cancer biomarker in biological samples with great potential for the fabrication of devices for clinical diagnostics.
研究领域:
分析化学
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生物化学
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纳米技术
·
生物医学工程
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临床诊断
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yumei Chen,Jialin Zhang,RuiRui Liu,Ying Zhang,Jingming Zhou,Hongliang Liu,Yankai Liu,Ke Yan,Yanhua Qi,Enping Liu,Xifang Zhu,Aiping Wang
A label-free electrochemical immunosensor was developed to rapidly detect tilmicosin (TMC) residues in pork and milk. The immunosensor was constructed by immobilizing a high-affinity monoclonal antibody against TMC on an rGO-PEI-Ag nanocomposite-modified electrode. The rGO-PEI-Ag nanocomposites were prepared by mixing polyethyleneimine (PEI) modified reduced graphene oxide (rGO) with AgNO 3 solution. The prepared rGO-PEI-Ag nanocomposites showed good redox activity and conductivity, as characterized by ultraviolet-visible spectroscopy (UV–Vis), transmission electron microscopy (TEM), and X-ray diffraction (XRD). During the preparation process, staphylococcal protein A (SPA) was added to targetedly bind the Fc segment of the monoclonal antibody. The immunosensor showed a low detection limit (LOD) of 0.0013 ng/mL, a linear range of 0.01–100 ng/mL, and recoveries ranging from 92.77 to 100.02% in pork and 92.26–101.23% in milk. Furthermore, the immunosensor exhibited good stability, reproducibility, and specificity in detecting TMC in pork and milk real samples.
研究领域:
分析化学
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食品科学
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纳米技术
·
生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Qian Liu,Xiaoying Yang,Longhui Luo,Wei Tian,Chao Kang,Tao Sun,Yuegang Chen,Juan Tao,Dongmei Chen
Benzimidazole fungicides are widely used in agriculture, and residues in food will threaten public health and safety. In this study, MXene@PEI@AgNPs composites were successfully constructed by electrostatic self-assembly method using polyethyleneimine (PEI) as a linker and used as a surface-enhanced Raman scattering (SERS) active substrate. The material combines the electromagnetic enhancement of silver nanoparticles and the chemical enhancement mechanism of MXene, showing excellent SERS performance. The experimental results show that the SERS substrate has excellent enhancement performance and successfully obtains the characteristic spectra of six target fungicides. After that, it was combined with chemometrics methods, through principal component analysis (PCA), it was found that the SERS spectra of different fungicides showed obvious clustering distribution. The constructed support vector machine (SVM) classification model shows excellent prediction performance, with an accuracy rate of 100 %. In terms of quantitative analysis, the method showed good sensitivity, and the detection recoveries of benzimidazole fungicides in actual samples such as kiwifruit, tomato and citrus were 81.62 % −113.87 %. Finally, in order to verify the reliability of the method, the high performance liquid chromatography (HPLC) method was used for comparative verification. The results showed that the two methods had good consistency.
研究领域:
分析化学
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食品安全
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纳米技术
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光谱学
·
农业科学
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2023
·
IF 12.6
Huanhuan Li,Wenhui Geng,Zhixiong Qi,Waqas Ahmad,Suleiman A. Haruna,Quansheng Chen
In this work, a stimuli-responsive SERS biosensor was fabricated for tetracycline (TTC) by “signal-on” strategy using (EDTA)-driven polyethyleneimine grafted calcium carbonate ( [email protected] 3 ) microcapsule and chitosan-Fe magnetic microbeads ( [email protected] ). Initially, aptamer conjugated magnetic-bead [email protected] @Apt with superparamagnetism and excellent biocompatibility was employed as capture probe, which facilitated the rapid and easy magnetic separation. Subsequently, the PEI cross-linked layer and aptamer network layer were constructed onto the outer layer of CaCO 3 @4-ATP microcapsule to form sensing probes ( [email protected] 3 @ [email protected] ) via the layer-by-layer assembly method. In the presence of TTC, a sandwich SERS-assay was exploited by aptamer recognition induced target-bridged strategy. When the solution of EDTA was added, the core layer of CaCO 3 would be dissolved quickly, destroying the microcapsule to release 4-ATP. The released 4-ATP could be quantitatively monitored by dripping the supernatant onto the [email protected] SERS platform, resulting in a strong Raman “signal-on”. Under the optimal conditions, a good linear relationship was established with a correlation coefficient ( R 2 ) of 0.9938 and a LOD of 0.03 ng/mL. Additionally, the application capacity of the biosensor to detect TTC was also affirmed in food matrixes, and the results were consistent with the standard ELISA method ( P > 0.05). Hence, this SERS biosensor affords extensive application prospects for TTC detection with multiple merits such as high sensitivity, environment friendliness, and high stability.
研究领域:
分析化学
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环境科学
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食品安全
·
生物传感器
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2022
·
IF 6.556
Haoyang He,Da-Wen Sun,Hongbin Pu,Zhihui Wu
Food aflatoxin B1 (AFB1) contamination greatly threatens human health and its sensitive determination is imperative. In this study, a surface-enhanced Raman scattering (SERS) and fluorescence dual-signal aptasensor was constructed for sensitive AFB1 detection in peanuts, walnuts, and almonds samples. Fluorescent dye cy5 was used as fluorophore and Raman reporter, while polyethyleneimine modified Ag coating magnetic nanoparticles (MNP@Ag-PEI) were utilized to absorb the cy5 modified aptamer (apt-cy5). Results indicated that linear ranges of 0.001–1000 ng/mL and 0.2–20,000 ng/mL with detection limits of 0.45 pg/mL and 0.135 ng/mL for the SERS and fluorescence methods were obtained, respectively, and AFB1 detection in the nut samples using the aptasensor achieved satisfactory recoveries of 95.2%–108.6% for SERS and 94.7%–109.7% for fluorescence. Compared with other mono signal detection, the established aptasensor facilely fused the merits of the two signals and improved the detection accuracy and flexibility.
研究领域:
分析化学
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食品科学
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纳米技术
·
毒理学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Xiaoqing Zhao,Jing Wang,Hou Chen,Hui Xu,Liangjiu Bai,Wenxiang Wang,Huawei Yang,Donglei Wei,Baiqing Yuan
A novel electrochemical immunoassay platform for multi-signal amplification has been constructed and applied to the simultaneous detection of three tumor markers (TMs). The platform was successfully developed by polyethyleneimine (PEI)-polydopamine (PDA)/reduced graphene oxide (rGO) nanocomposites. PDA/rGO as the substrates can simultaneously load with detection antibodies (Ab 2 ) and large amounts of PEI, which PEI were further used to immobilize different signal labels. Three TMs of carcinoembryonic antigen (CEA), prostate specific antigen (PSA) and alpha fetoprotein (AFP) were employed as potential factors. Ferrocenecarboxylic acid (Fc), anthraquinone-2-carboxylic acid (Aq) and acetylsalicylic acid (ASA) were employed as distinguishable signal labels. Electrochemical responses of designed sandwich-type immunosensor were successfully investigated by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The multiplexed immunoassay displayed excellent linear response in the range of 0.102 pg/mL–112 ng/mL for CEA and PSA, 0.117 pg/mL–117 ng/mL for AFP. The detection limit of three TMs were 91 fg/mL, 101 fg/mL and 83 fg/mL for CEA, PSA and AFP, respectively. Moreover, this electrochemical immunoassay platform exhibited excellent reproducibility, sensitivity and selectivity for the simultaneous detection of multiple biomarkers.
研究领域:
生物医学工程
·
分析化学
·
纳米技术
·
电化学
·
生物传感器
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）