MACROMOLECULAR RAPID COMMUNICATIONS
·
2024
·
IF 4.2
Huan Zhang,Meng Song,Shiya Zhuang,Zining Wang,Hui Shi,Zhuolang Song,Chuanhe Song,Lian Cen
With the continuous development of preventive and therapeutic vaccines, traditional adjuvants cannot provide sufficient immune efficacy and it is of high necessity to develop safe and effective novel nanoparticle-based vaccine adjuvants. α -Tocopherol (TOC) is commonly used in oil-emulsion adjuvant systems as an immune enhancer, yet its bioavailability is limited by poor water solubility. This study aims to develop TOC-loaded poly(lactic- co -glycolic acid) (PLGA) nanoparticles (TOC-PLGA NPs) to explore the potential of TOC-PLGA NPs as a novel nanoparticle-immune adjuvant. TOC-PLGA NPs are prepared by a nanoprecipitation method and their physicochemical properties are characterized. It is shown that TOC-PLGA NPs are 110.8 nm, polydispersity index value of 0.042, and Zeta potential of −13.26 mV. The encapsulation efficiency and drug loading of NPs are 82.57% and 11.80%, respectively, and the cumulative release after 35 days of in vitro testing reaches 47%. Furthermore, TOC-PLGA NPs demonstrate a superior promotion effect on RAW 264.7 cell proliferation compared to PLGA NPs, being well phagocytosed and also promoting antigen uptake by macrophages. TOC-PLGA NPs can strongly upregulate the expression of co-stimulatory surface molecules and the secretion of cytokines. In conclusion, TOC-PLGA NPs can be a novel vaccine adjuvant with excellent biocompatibility and significant immune-enhancing activity.
研究领域:
药剂学
·
免疫学
·
纳米技术
·
疫苗学
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2024
·Vol. 12
·期 27
·10135-10144
·
IF 5.7
Bairui Qi,Wenfang Wu,Ziyi Wang,Gengyan Liu,Zhou Li,Zhu Xiao
Chemodynamic therapy is an emerging therapeutic modality that utilizes the Fenton reaction for the production of reactive oxygen species to selectively target and kill cancer cells. In recent years, there has been a notable increase in the demand for developing nanomedicines with enhanced Fenton catalytic efficiency, self-generating H2O2 capability, and superior biocompatibility. This study focuses on the synthesis of a new nanomedicine, PLGA@carbon quantum dots-copper peroxide nanoparticles (PLGA@CQDs–CuPox NPs). The CQD nanodots exhibited the highest fluorescence intensity at an excitation wavelength of 400 nm. CuPox nanodots exhibited superior Fenton catalytic performance at a pH value of 5.5. In vitro experiments demonstrated that PLGA@CQDs–CuPox NPs achieved a killing rate of over 85% for tumor cells while showing a lower killing rate of 10% for normal cells. Upon entering tumor cells, PLGA@CQDs–CuPox NPs underwent degradation, releasing CQDs and CuPox nanodots. CQD nanodots emitted fluorescence, whereas CuPox nanodots decomposed to initiate a Fenton-like reaction. Through the enhanced permeability and retention effect, these nanoparticles penetrated deeper into the tumor cells, enabling deep-seated chemodynamic therapy. Uptake experiments further demonstrated that PLGA@CQDs–CuPox NPs escaped from lysosomes and entered the cell nucleus, utilizing the fluorescence changes of CQD nanodots to determine the optimal treatment time. The design of PLGA@CQDs–CuPox NPs offers a novel approach for precise cancer diagnosis and effective treatment through fluorescence imaging-guided chemodynamic therapy based on tumor microenvironment responsiveness.
研究领域:
纳米技术
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生物医学工程
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癌症研究
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材料科学
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产品关联
DOI
高影响因子
JOURNAL OF BIOMATERIALS APPLICATIONS
·
2019
·
IF 2.442
Juan Cai,Keyang Qian,Xueliang Zuo,Wuheng Yue,Yinzhu Bian,Ju Yang,Jia Wei,Wenying Zhao,Hanqing Qian,Baorui Liu
Docetaxel (TXT) is acknowledged as one of the most important chemotherapy agents for gastric cancer (GC). PI3K/AKT signaling is frequently activated in GC, and its inhibitor LY294002 exerts potent antitumor effects. However, the hydrophobicity of TXT and the poor solubility and low bioavailability of LY294002 limit their clinical application. To overcome these shortcomings, we developed poly(lactic acid/glycolic) (PLGA) nanoparticles loaded with TXT and LY294002. PLGA facilitated the accumulation of TXT and LY294002 at the tumor sites. The in vitro functional results showed that PLGA(TXT+LY294002) exhibited controlled-release and resulted in a markedly reduced proliferative capacity and an elevated apoptosis rate. An in vivo orthotopic GC mouse model and xenograft mouse model confirmed the anticancer superiority and tumor-targeting feature of PLGA(TXT+LY294002). Histological analysis indicated that PLGA(TXT+LY294002) was biocompatible and had no toxicity to major organs. Characterized by the combined slow release of TXT and LY294002, this novel PLGA-based TXT/LY294002 drug delivery system provides controlled release and tumor targeting and is safe, shedding light on the future of targeted therapy against GC.
研究领域:
药学
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肿瘤学
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纳米技术
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药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Nanomedicine
·
2025
·
IF 6.5
Qingyue Da,Min Xu,Yiting Tian,Huiping Ma,Haibo Wang,Linlin Jing
Background: Oxidative stress is considered an important mechanism of acute high-altitude brain injury. Imidazole nitronyl nitroxide radicals are a class of stable organic radical scavengers that contain single electrons in their molecules. Therefore, in order to search for compounds with low toxicity and better effect against high-altitude brain injury, the preparation methods of PLGA nanoparticles (TPP-C6-HPN@PLGA-NPs) loaded with a synthesized mitochondria targeting imidazole nitronyl nitroxide were emphasized and investigated. Furthermore, its protective effect on brain injury caused by low-pressure hypoxia (HH) in mice was evaluated.Methods: Nanoparticles were prepared by emulsion solvent evaporation method, and the preparation method was optimized by Box Behnken design based on particle size, encapsulation efficiency (EE) and drug loading (DL). Physical characterization and release studies of the optimized NPs were conducted. The high altitude brain injury mice model was selected to evaluate the therapeutic effect of TPP-C6-HPN@PLGA-NPs in vivo. The histological and biochemical tests were conducted in serum and brain of mice exposed to HH condition.Results: The nanoparticle size was 120.63 nm, the EE was 89.30%, the DL was 6.82%, the polydispersity index (PDI) was 0.172, and the zeta potential was − 22.67 mV under optimal preparation process. In addition, TPP-C6-HPN@PLGA-NPs owned good stabilities and sustained drug releases. TPP-C6-HPN@PLGA-NP exhibited lower toxicity than TPP-C6-HPN and was well uptaken by PC12 cells. Histological and biochemical analysis demonstrated that TPP-C6-HPN@PLGA-NPs significantly reduced HH induced pathological lesions, oxidative stress, energy dysfunction and inflammation response of brain tissue. Furthermore, nanoparticles did not show significant toxicity to major organs such as the liver and kidneys, as well as hematology in mice.Conclusion: TPP-C6-HPN@PLGA-NPs exhibits good stability, low hemolysis rate, sustained release, low toxicity, and long residence time in brain tissue and can be used as a promising formulation for the proper treatment of HH-induced brain damage.
研究领域:
药学
·
神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Artificial Cells Nanomedicine and Biotechnology
·
2019
·
IF 4.462
Chenghua Liang,Nan Li,Zikai Cai,Rongpu Liang,Xiaoming Zheng,Li Deng,Longbao Feng,Rui Guo,Bo Wei
At present, cancer is the first cause of death for humans, but early detection and treatment can help improve prognoses and reduce mortality. However, further development of carrier-assistant drug delivery systems (DDSs) is retarded by the aspects such as the low drug-carrying capacity, carrier-induced toxicity and immunogenicity, complex synthesis manipulation. The development of nanoscale drug delivery systems (NDDS) have been rapidly developed to address these issues. In this article, we used PLGA-PEG with good biocompatibility to encapsulate Fe3O4 nanoparticles (a magnetic resonance imaging contrast agent) and DOX (an antitumour drug) via the emulsion-solvent evaporation method, aimed at achieving a dual function of the early detection and the treatment of mammary cancer. The results showed that the Fe3O4/DOX/PLGA-PEG nanoparticles had a relatively uniform size, a high carrier rate of Fe3O4 and high encapsulation efficiency of DOX, and a relatively high activity of released DOX within 120 h. In addition, in vitro studies showed that the Fe3O4/DOX/PLGA-PEG nanoparticles were cytocompatibility in NIH 3T3 fibroblast cells culture study while had a special effect on destroying human breast cancer MCF-7 cells compared with pure DOX solution. In vitro studies revealed that the Fe3O4/DOX/PLGA-PEG enabled enhanced T2 contrast magnetic resonance. Overall, our multifunctional Fe3O4/DOX/PLGA-PEG nanoparticles, composed of biocompatible substances and therapeutic/imaging materials, have great potential for the early detection of cancer and accurate drug delivery via the dynamic monitoring using MRI.
研究领域:
生物医学工程
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纳米医学
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肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMEDICINE & PHARMACOTHERAPY
·
2025
·
IF 7.5
Fatemeh Ajalloueian,Atena Malakpour-Permlid,Marzieh Yeganegi,Somayeh Azizi,Aleka Vasos,Gohar Soufi,Anja Boisen
In this study, we addressed the challenge of enhancing the efficacy of curcumin (Cur) delivery for cancer treatment by developing Cur-loaded electrospun sheets (ES) fabricated from PLGA (poly(lactic-co-glycolic acid)) with a focus on tailoring both the dosage and the physical dimensions of the delivery device to suit specific therapeutic applications. Two formulations containing 10 % and 50 % Cur by weight relative to PLGA were designed and produced. We demonstrated that both formulations maintained the amorphous structure of PLGA, where increasing the Cur concentration enhanced the sheets' wettability, tensile extensibility, and mucoadhesive properties. Comparing the drug release trends and the cytotoxicity of the Cur-loaded ES against HeLa cells (human cervical cancer) revealed that both the Cur dosage and the sheet dimensions influenced drug release kinetics and anticancer efficacy. Hence, we highlight the importance of considering specific size-dose relevance measurements when applying polymer-drug systems in solid form. For instance, when maintaining the same dose, a smaller segment of the high dose sheet is suitable for oral delivery, thanks to the higher drug content and better mucoadhesion properties. The low dosage sheet enjoying a larger surface area, could fit local applications requiring uniform static delivery, such as post-surgery recurrence inhibition dressing.
✓ 已验证
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2018
·Vol. 6
·期 22
·3655-3664
·
IF 4.776
Yijia Tang,Siming Huang,Jianqiao Xu,Gangfeng Ouyang,Yuan Liu
Nanomaterials have shown great potential for application in microextraction due to their distinguishing nanoscale architectures and superior physicochemical properties. Herein, novel poly(lactic-co-glycolic acid) (PLGA) solid-phase microextraction (SPME) fibers, which were incorporated with a self-assembled graphene oxide (GO)-coated γ-Al2O3 composite (Al2O3@GO), were fabricated on stainless steel wires via an electrospinning method. The as-spun nanofibers were further sheathed through the self-polymerization of noradrenaline (NA), an agonist found in oysters, to provide a compatible biointerface and antifouling capacity. Acting as the coating substrate of the as-prepared fibers, PLGA is known for its prominent biocompatibility and biodegradability, while the adsorptive Al2O3@GO particles helped to increase their loading capacity. The modified PLGA-based electrospun nanofibers exhibited much higher extraction efficiency compared with the thicker polydimethylsiloxane (PDMS) coatings (165 μm) and polyacrylate (PA) coatings (85 μm). Due to the fine biointerface of the PLGA-based nanofibers, a rapid extraction equilibrium was observed and sampling with the custom-made Al2O3@GO–PLGA@PNA fibers could be accomplished within 15 min. The fibers were then successfully employed for simultaneous in vivo sampling of tetrodotoxin (TTX) and sulfonamides (SAs) in the dorsal–epaxial muscle of living pufferfish (Takifugu obscurus), and satisfactory sensitivities with limits of detection (LODs) in the range of 0.52–2.30 ng g−1 and comparable accuracies to the conventional liquid extraction (LE) method were achieved. In vivo sampling of target pharmaceuticals with the modified nanofibers showed their feasibility for further metabolomics and pharmacokinetics studies in biotissues.
研究领域:
生物医学工程
·
分析化学
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产品关联
DOI
PubMed
高影响因子
Nanoscale Research Letters
·
2018
·Vol. 13
·期 1
·1-10
·
IF 3.125
Liang Chunyong,Luo Yongchao,Yang Guodong,Xia Dan,Liu Lei,Zhang Xiaomin,Wang Hongshui
Biodegradable porous biomaterial scaffolds play a critical role in bone regeneration. In this study, the porous nano-hydroxyapatite/collagen/poly(lactic-co-glycolic acid)/graphene oxide (nHAC/PLGA/GO) composite scaffolds containing different amount of GO were fabricated by freeze-drying method. The results show that the synthesized scaffolds possess a three-dimensional porous structure. GO slightly improves the hydrophilicity of the scaffolds and reinforces their mechanical strength. Young’s modulus of the 1.5 wt% GO incorporated scaffold is greatly increased compared to the control sample. The in vitro experiments show that the nHAC/PLGA/GO (1.5 wt%) scaffolds significantly cell adhesion and proliferation of osteoblast cells (MC3T3-E1). This present study indicates that the nHAC/PLGA/GO scaffolds have excellent cytocompatibility and bone regeneration ability, thus it has high potential to be used as scaffolds in the field of bone tissue engineering.
研究领域:
材料科学
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生物材料
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组织工程
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Regenerative Therapy
·
2024
·
IF 3.4
Min Ge,Li Sun,Defeng Wang,Chunchao Hei,Tingjuan Huang,Zhongxin Xu,Qizhi Shuai
The use of stem cell-based treatment systems is prevalent in regenerative medicine. To enhance the regenerative capabilities of stem cells, growth factors are typically incorporated into the treatment system. Nonetheless, traditional hydrogel-encapsulated or heparinized scaffolds that bind factors have limitations. In this study, we prepared a biomaterial strategy using uniform poly(lactic-co-glycolic) acid (PLGA) microspheres (uPLGA-Ms) fabricated by microfluidic to sustain delivery of insulin-like growth factor 1 (IGF-1), a critical protein for hMSCs biological functions. The uPLGA-Ms loaded IGF-1 were highly monodispersed through precise manipulation of the flow rate of the two-phase of the flow-focusing microchannle. The results showed that the uPLGA-Ms stabilize IGF-1 and provide a more efficient sustained delivery and cost-effective of growth factor. Gene expression analysis demonstrated the uPLGA delivery of IGF-1 results in a (enhanced) supported hMSCs expansion, survival, stemness, and secretion abilities comparable with the conventional soluble IGF-1 group. In summary, this material-based strategy to stabilize and sustain delivery of growth factor has broad potential to regeneration of various tissues and organs.
研究领域:
再生医学
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生物材料
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干细胞治疗
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组织工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
·
2019
·Vol. 30
·期 2
·1-11
·
IF 2.467
Fan Wei,Li Yanyun,Liu Danfeng,Sun Qing,Duan Mengting,Fan Bing
Enterococcus faecalis ( E. faecalis ), a Gram-positive facultative anaerobe, is reported to take responsibility for a large portion of refractory root canal infections and root canal re-infections of human teeth. Chlorhexidine is a strong bactericide against E. faecalis but cannot infiltrate into dentinal tubules. On the other hand, a common negative effect of root canal medicaments is the decrease of dentin microhardness. In this study, poly(D,L-lactic- co -glycolide) (PLGA) submicron particles were applied as delivery carriers to load and release the chlorhexidine as well as calcium and phosphorus. The release profiles, antibacterial ability against E. faecalis , infiltration ability into dentinal tubules, biocompatibility and effects on dentin microhardness of these particles were investigated. Results revealed that encapsulated chemicals could be released in a sustained manner from the particles. The particles also exhibited excellent biocompatibility on MC3T3-E1 cells and significant antimicrobial property against E. faecalis . On dentin slices, the particles could be driven into dentinal tubules by ultrasonic activiation and inhibit E. faecalis colonization. Besides, dentin slices medicated with the particles displayed an increase in microhardness. In conclusion, PLGA submicron particles carrying chlorhexidine, calcium and phosphorus could be developed into a new intra-canal disinfectant for dental treatments.
研究领域:
牙科学
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药学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2018
·Vol. 8
·期 1
·1-18
·
IF 4.122
Du Jing,Li Xiao-Yu,Hu He,Xu Li,Yang Shi-Ping,Li Feng-Hua
Molecularly-targeted contrast enhanced ultrasound (US) imaging is a promising imaging strategy with large potential for improving diagnostic accuracy of conventional US imaging in breast cancer detection. Therefore, we constructed a novel dual-targeted nanosized US contrast agent (UCA) directed at both vascular endothelial growth factor receptor 2 (VEGFR2) and human epidermal growth factor receptor 2 (HER2) based on perfluoropropane (C 3 F 8 )-filled poly(lactic-co-glycolic acid) (PLGA) (NBs) for breast cancer detection. In vitro , single- or dual-targeted PLGA NBs showed high target specificities and better effects of target enhancement in VEGFR2 or HER2-positive cells. In vivo , US imaging signal in the murine breast cancer model was significantly higher ( P < 0.01) for dual-targeted NBs than single-targeted and non-targeted NBs. Small animal fluorescence imaging further confirmed the special affinity of the dual-targeted nanosized contrast agent to both VEGFR2 and HER2. Immunofluorescence and immunohistochemistry staining confirmed the expressions of VEGFR2 and HER2 on tumor neovasculature and tumor cells of breast cancer. In conclusions, the feasibility of using dual-targeted PLGA NBs to enhance ultrasonic images is demonstrated in vitro and in vivo . This may be a promising approach to target biomarkers of breast cancer for two site-specific US molecular imaging.
研究领域:
医学影像
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肿瘤学
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药剂学
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BioNanoScience
·
2025
·Vol. 15
·期 4
·1-11
·
IF 3.2
Heng Youqiang,Du Zhong,Chu Chenyang,Xiong Jiabao,Gao Yuxiang,Ma Rong,Wang Jing,Alifu Nuernisha,Ma Cailing
Cervical cancer remains one of the leading cause of cancer death in women. As the development of nanomedicine, multifunctional nanoplatforms which integrate imaging and therapy for effective diagnostics and therapeutics of cancer have attracted great attention in recent years. In this work, we have designed and developed a kind of multimodal imaging and treatment strategy which combined near-infrared (NIR) fluorescence imaging with magnetic resonance imaging (MRI) as well as photothermal therapy (PTT) via a multifunctional nanoplatform. The poly lactic-co-glycolic acid (PLGA) was utilized to encapsulate magnetic Fe 3 O 4 nanoparticles (NPs) and NIR fluorescent dye clinical indocyanine green (ICG), by which integrated the advantages of Fe 3 O 4 NPs and clinical ICG under 808-nm laser excitation, to achieve highly efficient dual-modal imaging-guided cervical tumor ablation. This work provides a promising approach for multimodal imaging diagnosis and PTT of cervical cancer.
研究领域:
肿瘤学
·
纳米医学
·
分子影像学
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光热治疗
·
转化医学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）